UV-Protection Effect of Nanoemulgel Formulation from Moringa oleifera Lam. Leaf Extracts in Rattus novergicus

Authors

Arie Tika Anditha , Linda Chiuman , Chrismis Novalinda Ginting

DOI:

10.29303/jppipa.v10i9.8497

Published:

2024-09-25

Issue:

Vol. 10 No. 9 (2024): September

Keywords:

Histology, Melanin, Moringa oleifera, Nanomaterials, Photoprotection

Research Articles

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Anditha, A. T., Chiuman, L., & Ginting, C. N. (2024). UV-Protection Effect of Nanoemulgel Formulation from Moringa oleifera Lam. Leaf Extracts in Rattus novergicus. Jurnal Penelitian Pendidikan IPA, 10(9), 6603–6611. https://doi.org/10.29303/jppipa.v10i9.8497
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Abstract

Exposure to sunlight (UV radiation) can harm the skin, leading to cancer and tissue damage. The body’s natural defense involves activating hormones that stimulate melanin synthesis to reduce cellular damage. Moringa oleifera Lam. leaves, rich in flavonoids and phenolic compounds, are promising UV-protection agents. This study assessed the efficacy of nanoemulgel formulations containing M. oleifera leaf extract through in vitro and in vivo assays. Twenty-five rats were divided into five groups, including formulations with 2%, 3%, and 4% M. oleifera extract, a negative control, and a positive control with Azarine Sunscreen Gel SPF 45. The nanoemulsions had appropriate size and quality, with the highest SPF observed at 4% concentration (7.95±0.06). According to FDA classification, these formulations fall within the 'extra' SPF category (6<SPF<8). However, melanin scores in the nanoemulgel groups were comparable to the untreated control, and the 4% concentration (44.4±16.3) did not match the protection provided by commercial sunscreen (10.0±1.58). These findings suggest M. oleifera has potential for photoprotection, but further optimization is needed.

References

Afriza, Z. N., Muhani, N., Sari, N., & Sari, F. E. (2023). Use of moringa leaves to meet toddler nutrition in the prevention of stunting. Jurnal Penelitian Pendidikan IPA, 9(12), 11333-11341. https://doi.org/10.29303/jppipa.v9i12.4325

Amelia, Girsang, E., Nasution, A. N., & Ginting, C. N. (2021). Anti-aging effectiveness of red spinach extract ointment (amaranthus tricolor l.) against collagen, elasticity, hydration, sebum, and pigment levels in wistar rats. 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce), 1–6. https://doi.org/10.1109/InHeNce52833.2021.9537221

Baldisserotto, A., Buso, P., Radice, M., Dissette, V., Lampronti, I., Gambari, R., Manfredini, S., & Vertuani, S. (2018). Moringa oleifera leaf extracts as multifunctional ingredients for “natural and organic” sunscreens and photoprotective preparations. Molecules, 23(3), 664. https://doi.org/10.3390/molecules23030664

Balkrishna, A., Singh, S., Srivastava, D., Mishra, S., Sharma, S., Mishra, R., & Arya, V. (2023). A systematic review on traditional, ayurvedic, and herbal approaches to treat solar erythema. International Journal of Dermatology, 62(3), 322–336. https://doi.org/10.1111/ijd.16231

Binder, L., Mazál, J., Petz, R., Klang, V., & Valenta, C. (2019). The role of viscosity on skin penetration from cellulose ether-based hydrogels. Skin Research and Technology, 25(5), 725–734. https://doi.org/10.1111/srt.12709

Brenner, M., & Hearing, V. J. (2008). The protective role of melanin against uv damage in human skin. Photochemistry and Photobiology, 84(3), 539–549. https://doi.org/10.1111/j.1751-1097.2007.00226.x

Carvalho, P. G., Naveira, R. I., Granone, L. I., Mendive, C. B., Massa, A. E., & Churio, M. S. (2023). A comparative review of natural and synthetic UV filters: Gadusol and benzophenone-3 as representative examples. Environmental Advances, 13, 100404. https://doi.org/10.1016/j.envadv.2023.100404

Das, S., Murugavel, K. G., Karpagavel, L., & Manikandan, N. (2020). A study to formulate the synthesis and characterization of Tri- herbal leaf extracts nanoemulsion. Annals of Tropical Medicine and Public Health, 23(20). https://doi.org/10.36295/asro.2020.232230

Djunaidy, A., & Damayanti, P. A. A. (2023). Bombyx mori as a potential sunscreen to prevent photoaging. Jurnal Penelitian Pendidikan IPA, 9(11), 1209-1220. https://doi.org/10.29303/jppipa.v9i11.5507

Gaikwad, M., & Kale, S. (2011). Formulation and in vitro evaluation for sun protection factor of Moringa oleifera Lam (Family-Moringaceae) oil sunscreen cream. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4), 371–375. Retrieved from https://www.innovareacademics.in/journal/ijpps/Vol3Issue4/2683.pdf

Garnacho Saucedo, G. M., Salido Vallejo, R., & Moreno Giménez, J. C. (2020). Effects of solar radiation and an update on photoprotection. Anales de Pediatría (English Edition), 92(6), 377.e1-377.e9. https://doi.org/10.1016/j.anpede.2020.04.003

Gimenis, J. M., Gomes, A. C., Santos, V. H. M. dos, Ferreira, P. C., Oliveira, C. A., Baby, A. R., & Silva, R. M. G. (2018). Antioxidant and photoprotective potential of Moringa oleifera Lam (Moringaceae). Bioscience Journal, 34(5), 1365-1378. https://doi.org/10.14393/BJ-v34n5a2018-39845

Girsang, E., Ginting, C. N., Lister, I. N. E., Gunawan, K. yashfa, & Widowati, W. (2021). Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell. PeerJ, 9, e11419. https://doi.org/10.7717/peerj.11419

Handayani, S. S., Gunawan, E. R., Suhendra, D., Murniati, M., Hermanto, D., & Karnila, L. (2022). Utilization of Moringa seed oil fatty acids for azelaic acid base stock production. Jurnal Penelitian Pendidikan IPA, 8(5), 2319-2323. https://doi.org/10.29303/jppipa.v8i5.1964

Harahap, E. S., Ginting, C. N., Lubis, Y. M., & Chiuman, L. (2022). The activity of strawberry extract as sunscreen on guinea pigs exposed to sunlight. IOP Conference Series: Earth and Environmental Science, 1083(1), 012007. https://doi.org/10.1088/1755-1315/1083/1/012007

Hassan, M. A., Xu, T., Tian, Y., Zhong, Y., Ali, F. A. Z., Yang, X., & Lu, B. (2021). Health benefits and phenolic compounds of Moringa oleifera leaves: A comprehensive review. Phytomedicine, 93, 153771. https://doi.org/10.1016/j.phymed.2021.153771

Irawan, D., Ramadhan, K., & Azhar, A. (2022). Design of PCF-SPR for early detection of skin cancer infected cells. Jurnal Penelitian Pendidikan IPA, 8(5), 2293-2298. https://doi.org/10.29303/jppipa.v8i5.2120

Irimpan, L. M. (2020). A comparative study on some natural and synthetic sunscreens and their skin aberration measurements. International Journal of Science and Research, 10(12), 772-775. Retrieved from https://www.ijsr.net/archive/v10i12/SR211214080921.pdf

Isnaini, N., Prajaputra, V., & Maryam, S. (2023). Formulation and evaluation of o/w body cream containing patchouli oil (Pogostemon cablin Benth.) and drumstick oil (Moringa oleifera) as potential moisturizing agent. Jurnal Penelitian Pendidikan IPA, 9(10), 8001-8007. https://doi.org/10.29303/jppipa.v9i10.4292

Jimtaisong, N. S. A. (2013). Photoprotection of natural flavonoids. Journal of Applied Pharmaceutical Science, 3(9), 129–141. https://doi.org/10.7324/JAPS.2013.3923

Jusnita, N., & Nasution, K. (2019). Formulasi nanoemulsi ekstrak daun kelor (Moringa oleifera Lamk). Industria: Jurnal Teknologi dan Manajemen Agroindustri, 8(3), 165-170. https://doi.org/10.21776/ub.industria.2019.008.03.1

Latcuba, S. P., Chiuman, L., Nasution, A. N., & Ginting, C. N. (2022). Analysis of wound healing activity from rose petal extract ointment in wistar rats. IOP Conference Series: Earth and Environmental Science, 1083(1), 012047. https://doi.org/10.1088/1755-1315/1083/1/012047

Lister, I. N. E., Ginting, C. N., Girsang, E., Amansyah, A., Chiuman, L., Yanti, N. L. W. E., Widodo, W. S., Yusepany, D. T., Rizal, R., & Widowati, W. (2022). Piper crocatum ameliorates APAP-Induced hepatotoxicity through antioxidant and anti-inflammatory mechanisms. Journal of Mathematical and Fundamental Sciences, 54(1), 109-120. https://doi.org/10.5614/j.math.fund.sci.2022.54.1.6

Liu, R., Liu, J., Huang, Q., Liu, S., & Jiang, Y. (2022). Moringa oleifera: A systematic review of its botany, traditional uses, phytochemistry, pharmacology and toxicity. Journal of Pharmacy and Pharmacology, 74(3), 296–320. https://doi.org/10.1093/jpp/rgab131

Lourith, N., Kanlayavattanakul, M., & Ruktanonchai, U. (2016). Formulation and stability of Moringa oleifera oil microemulsion. Soft Materials, 14(2), 64–71. https://doi.org/10.1080/1539445X.2016.1141786

Lubis, A., Wardhani, F. M., Lubis, A. T., & Tandanu, E. (2023). Cerebral histopathology in acute toxicity test of Curcuma zedoria. Jurnal Penelitian Pendidikan IPA, 9(10), 8368-8375. https://doi.org/10.29303/jppipa.v9i10.5482

Lukić, M., Pantelić, I., & Savić, S. D. (2021). Towards optimal ph of the skin and topical formulations: From the current state of the art to tailored products. Cosmetics, 8(3), 69. https://doi.org/10.3390/cosmetics8030069

Maddodi, N., Jayanthy, A., & Setaluri, V. (2012). Shining light on skin pigmentation: the darker and the brighter side of effects of uv radiation. Photochemistry and Photobiology, 88(5), 1075–1082. https://doi.org/10.1111/j.1751-1097.2012.01138.x

Maldini, M., Maksoud, S. A., Natella, F., Montoro, P., Petretto, G. L., Foddai, M., De Nicola, G. R., Chessa, M., & Pintore, G. (2014). Moringa oleifera: Study of phenolics and glucosinolates by mass spectrometry. Journal of Mass Spectrometry, 49(9), 900–910. https://doi.org/10.1002/jms.3437

Mansur, J. de S., Breder, M. N. R., Mansur, M. C. d’Ascençäo, & Azulay, R. D. (1986). Determinaçäo do fator de proteçäo solar por espectrofotometria. Anais Brasileiros de Dermatologia, 61(3), 121–124. Retrieved from https://pesquisa.bvsalud.org/portal/resource/pt/lil-34224

Mattar, A. A., El-Desouky, S. Ma., Elrefaey, A. A. E.-A., Abd El-Raouf, W., & Soliman, T. N. (2024). Preparing and evaluating a caseinate-based M. oleifera seed oil nanoemulsion to be incorporated into functional ice cream. Egyptian Journal of Chemistry, 67(9), 173–184. https://doi.org/10.21608/ejchem.2024.239300.8679

Muharraran, F., Erawati, S., & Dalimunthe, D. A. L. (2023). Comparison of the antibacterial effectiveness of moringa leaf extract (Moringa oleifera) on the growth of Enterococcus faecalis and Streptococcus mutans bacteria in vitro. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 1372-1377. https://doi.org/10.29303/jppipa.v9iSpecialIssue.7715

Pakade, V., Cukrowska, E., & Chimuka, L. (2013). Comparison of antioxidant activity of Moringa oleifera and selected vegetables in South Africa. South African Journal of Science, 109(3), 1–5. https://doi.org/10.10520/EJC133141

Pelleg, J. (2021). What Are Nanomaterials and Why the Interest in It. In J. Pelleg (Ed.), Mechanical Properties of Nanomaterials (pp. 1–5). Springer International Publishing. https://doi.org/10.1007/978-3-030-74652-0_1

Prasad Rajbhar, Y., Rajbhar, G., Pl, R., Shukla, S., & Kumar, M. (2018). Grow Moringa (Moringa oleifera), the miracle tree on the earth. Horticulture International Journal, 2(4), 166-172. https://doi.org/10.15406/hij.2018.02.00047

Proksch, E. (2018). pH in nature, humans and skin. The Journal of Dermatology, 45(9), 1044–1052. https://doi.org/10.1111/1346-8138.14489

Santos, V. M. R. D., Mota, G. C. F., Campos, K., Sousa, L., Amparo, T., Vieira, P., Okuma, A., & Melo, T. (2021). Evaluation of the, in vitro, photoprotetive capacity of Moringa oleifera oil for its use in sunscreen formulation. Revista de Biotecnologia & Ciência, 10(2), 14-20. https://doi.org/10.31668/rbc.v10i2.11909

Sayre, R. M., Desrochers, D. L., Marlowe, E., & Urbach, F. (1978). The correlation of indoor solar simulator and natural sunlight: Testing of a sunscreen preparation. Archives of Dermatology, 114(11), 1649–1651. https://doi.org/10.1001/archderm.1978.01640230023007

Setyowiranti, M. F., & Darmaputra, I. G. N. (2023). Snakehead fish extract in increasing skin collagen & fibroblasts. Jurnal Penelitian Pendidikan IPA, 9(11), 1189-1197. https://doi.org/10.29303/jppipa.v9i11.5496

Shah, P., Bhalodia, D., & Shelat, P. (2010). Nanoemulsion: A pharmaceutical review. Systematic Reviews in Pharmacy, 1(1), 24. https://doi.org/10.4103/0975-8453.59509

Siregar, L., Chiuman, L., & Wienaldi. (2024). Potential Dutch eggplant peel (Solanum betaceum) as H2O2 scavenging and antiaging activity. African Journal of Biological Sciences, 6(8), 1608–1615. Retrieved from https://www.afjbs.com/uploads/paper/1404938de5003a277e272ce7092d6729.pdf

Stohs, S. J., & Hartman, M. J. (2015). Review of the safety and efficacy of Moringa oleifera. Phytotherapy Research, 29(6), 796–804. https://doi.org/10.1002/ptr.5325

Suyono, T., Mucmaini, R., Ginting, C. N., & Pratama, I. H. (2023). Dermapen action on the skin of white rats wistar strain (Rattus norvegicus) to evaluate the efficacy of avocado fruit ethanol extract cream (Persea americana Mill.) in Healing Wounds. Jurnal Penelitian Pendidikan IPA, 9(12), 11697-11704. https://doi.org/10.29303/jppipa.v9i12.6181

Tobing, Y., Chiuman, L., Ginting, S., & Ginting, C. (2020). The Effect of black cumin extract cream (Nigella sativa) on melanin pigment level in rats (Rattus norvegicus) exposed with ultraviolet-B rays. International Journal of Research in Pharmaceutical Sciences, 11, 5221–5224. https://doi.org/10.26452/ijrps.v11i4.3133

Varsha, G., Pooja, B., & Pournima, S. (2018). Anti-solar study of ethanolic extract of leaves Moringa oleifera. International Journal of Advanced Community Medicine, 1(3), 08–10. https://doi.org/10.33545/comed.2018.v1.i3a.23

Wijoyo, S., & Wahyuniari, I. A. I. (2023). The potency of passion fruit (Passiflora edulis) prevents UV-induced skin aging. Jurnal Penelitian Pendidikan IPA, 9(11), 1142-1147. https://doi.org/10.29303/jppipa.v9i11.5483

Xie, M., Jiang, Z., Lin, X., & Wei, X. (2024). Application of plant extracts cosmetics in the field of anti-aging. Journal of Dermatologic Science and Cosmetic Technology, 1(2), 100014. https://doi.org/10.1016/j.jdsct.2024.100014

Yamaguchi, Y., & Hearing, V. J. (2006). Melanocyte Distribution and Function in Human Skin. In V. J. Hearing & S. P. L. Leong (Eds.), From Melanocytes to Melanoma: The Progression to Malignancy (pp. 101–115). Humana Press. https://doi.org/10.1007/978-1-59259-994-3_6

Author Biographies

Arie Tika Anditha, Universitas Prima Indonesia

Linda Chiuman, Universitas Prima Indonesia

Chrismis Novalinda Ginting, Universitas Prima Indonesia

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