Isolation and Identification of Swan (Cygnus olor) Digestive Tract Cellulothic Bacteria to Support Fiber Degradation

Authors

Muhammad Subhan Bahruddin Rosyidi , Muhammad Ali , Wayan Wariata , Djoko Kisworo , Khairunnisah , Qothrunnada Amira Salma , Muhammad Hipzul Mursyid

DOI:

10.29303/jppipa.v10iSpecialIssue.8516

Published:

2024-08-25

Issue:

Vol. 10 No. SpecialIssue (2024): Science Education, Ecotourism, Health Science

Keywords:

Cellulase enzyme, Cellulolytic, Fiber degradation, Swan

Research Articles

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Rosyidi, M. S. B., Ali, M., Wariata, W., Kisworo, D., Khairunnisah, Salma, Q. A., & Mursyid, M. H. (2024). Isolation and Identification of Swan (Cygnus olor) Digestive Tract Cellulothic Bacteria to Support Fiber Degradation. Jurnal Penelitian Pendidikan IPA, 10(SpecialIssue), 572–578. https://doi.org/10.29303/jppipa.v10iSpecialIssue.8516
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Abstract

Cellulolytic bacteria are bacteria that produce cellulase enzymes capable of degrading cellulose substrates. This study aimed to isolate and identify as well as measure the cellulase enzyme activity of cellulolytic bacteria isolated from the digestive tract of swans (Cygnus olor) in Mataram. The bacteria were isolated using de Man Rogosa Sharpe (MRS) selective media with the spread plate method. Then, the extracellular enzyme activity test was conducted by growing selected pure isolates on 1% Carboxy Methyl Cellulose (CMC 1%) media, followed by pouring 0.1% congo red solution and rinsing with 1M NaCl solution to determine cellulolytic activity (cellulolytic potential indicated by the appearance of a clear zone around the colony) and measuring the cellulolytic index. The bacterial isolation results obtained 4 isolates with the potential to support fiber degradation in animal feed. The highest cellulolytic index was produced by the isolate coded S1, reaching 34.5 mm, while the lowest cellulolytic index was produced by 2 isolates coded J21 and I2, reaching 18.8 mm.

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Author Biographies

Muhammad Subhan Bahruddin Rosyidi, Universitas Mataram

Muhammad Ali, Universitas Mataram

Wayan Wariata, Universitas Mataram

Djoko Kisworo, Universitas Mataram

Khairunnisah, Universitas Mataram

Qothrunnada Amira Salma, Universitas Mataram

Muhammad Hipzul Mursyid, University of Mataram

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Copyright (c) 2024 Muhammad Subhan Bahruddin Rosyidi, Muhammad Ali, Wayan Wariata, Djoko Kisworo, Khairunnisah, Qothrunnada Amira Salma, Muhammad Hipzul Mursyid

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