The Effect of Giving Forest Guava Fruit Yogurt (Bellucia pentamera Naudin) on the Growth of Esterichia coli Bacteria in Mice (Mus musculus)
DOI:
10.29303/jppipa.v11i2.9077Published:
2025-02-25Issue:
Vol. 11 No. 2 (2025): FebruaryKeywords:
E. coli, Jambu hutan (Bellucia pentamera Naudin), Mencit (Mus musculus), YoghurtResearch Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
This research investigates the effect of forest guava fruit yogurt (Bellucia pentamera Naudin) on the growth of Escherichia coli bacteria in mice (Mus musculus). The study utilized an experimental design with treatment and control groups. Mice were divided into four groups: three treatment groups received forest guava fruit yogurt at concentrations of 5, 10, and 15%, respectively, administered at 0.5 ml per dose daily for 14 days, while the control group was given plain yogurt. E. coli growth was assessed by analyzing fecal samples from each group. The findings revealed that the growth of E. coli bacteria in the mice decreased significantly after administration of forest guava fruit yogurt, with the most pronounced reduction observed in the group receiving the 15% concentration. In contrast, the control group, which received plain yogurt, exhibited a less marked decrease in E. coli growth. These results suggest that forest guava fruit yogurt has a stronger inhibitory effect on E. coli bacteria compared to plain yogurt. In conclusion, forest guava fruit yogurt appears to be an effective natural agent for reducing E. coli growth in mice, potentially offering added health benefits beyond those of conventional yogurt.
References
Agusanty, S. F., Mulyanita, M., & Suaebah, S. (2023). Analisis Kandidat Pangan Lokal Buah Jambu Hutan (Bellucia Pentamera Naudin) Sebagai Sumber Prebiotik. Pontianak Nutrition Journal (PNJ), 6(1). https://doi.org/10.30602/pnj.v6i1.1140
Anumudu, C. K., Miri, T., & Onyeaka, H. (2024). Multifunctional Applications of Lactic Acid Bacteria: Enhancing Safety, Quality, and Nutritional Value in Foods and Fermented Beverages. Foods, 13(23). https://doi.org/10.3390/foods13233714
Arabski, M., Węgierek-Ciuk, A., Czerwonka, G., Lankoff, A., & Kaca, W. (2012). Effects of saponins against clinical E. coli strains and eukaryotic cell line. Journal of Biomedicine & Biotechnology, 2012, 286216. https://doi.org/10.1155/2012/286216
Ayu Rochmiyah, F., Finatsiyatull Rosida, D., & Yusuf, A. (2022). Characteristics of Guava Probiotic Drink on Various Fruit Type and Fermentation Time. Food Science and Technology Journal (Foodscitech), 2004, 106–113. https://doi.org/10.25139/fst.vi.5245
Basavaraju, M., & Gunashree, B. S. (2022). Escherichia coli: An Overview of Main Characteristics. In Escherichia coli. IntechOpen. https://doi.org/10.5772/intechopen.105508
Cankaya, I. I. T., & Somuncuoglu, E. I. (2021). Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents against Respiratory Tract Infections. Evidence-Based Complementary and Alternative Medicine : ECAM, 2021, 6814215. https://doi.org/10.1155/2021/6814215
Crane, R. J., Jones, K. D. J., & Berkley, J. A. (2015). Environmental enteric dysfunction: an overview. Food and Nutrition Bulletin, 36(1), S76-87. https://doi.org/10.1177/15648265150361S113
de Onis, M., & Branca, F. (2016). Childhood stunting: a global perspective. Maternal & Child Nutrition, 12 Suppl 1(1), 12–26. https://doi.org/10.1111/mcn.12231
De Sanctis, V., Soliman, A., Alaaraj, N., Ahmed, S., Alyafei, F., & Hamed, N. (2021). Early and Long-term Consequences of Nutritional Stunting: From Childhood to Adulthood. Acta Bio-Medica : Atenei Parmensis, 92(1), e2021168. https://doi.org/10.23750/abm.v92i1.11346
Dempsey, E., & Corr, S. C. (2022). Lactobacillus spp. for Gastrointestinal Health: Current and Future Perspectives. Frontiers in Immunology, 13, 840245. https://doi.org/10.3389/fimmu.2022.840245
Dewi, A. K., Mahendra, G. D., & Ambarukmi, F. (2023). Studying the influence of guava fruitghurt on Escherichia coli coloniesin rats’ digestive tracts. ACTA Medical Health Sciences, 2(2), 73-80. https://doi.org/10.35990/amhs.v2n2.p73-80
Gabain, I. L., Ramsteijn, A. S., & Webster, J. P. (2023). Parasites and childhood stunting – a mechanistic interplay with nutrition, anaemia, gut health, microbiota, and epigenetics. Trends in Parasitology, 39(3), 167–180. https://doi.org/10.1016/j.pt.2022.12.004
Harper, K. M., Mutasa, M., Prendergast, A. J., Humphrey, J., & Manges, A. R. (2018). Environmental enteric dysfunction pathways and child stunting: A systematic review. PLoS Neglected Tropical Diseases, 12(1), 1–23. https://doi.org/10.1371/journal.pntd.0006205
Hutasoit, D. P. (2020). Pengaruh Sanitasi Makanan dan Kontaminasi Bakteri Escherichia coli Terhadap Penyakit Diare. Jurnal Ilmiah Kesehatan Sandi Husada, 12(2), 779–786. https://doi.org/10.35816/jiskh.v12i2.399
Li, C., Song, J., Kwok, L., Wang, J., Dong, Y., Yu, H., Hou, Q., Zhang, H., & Chen, Y. (2017). Influence of Lactobacillus plantarum on yogurt fermentation properties and subsequent changes during postfermentation storage. Journal of Dairy Science, 100(4), 2512–2525. https://doi.org/10.3168/jds.2016-11864
Marisa, H. & Salni. (2023). Bellucia pentamera Naudin Potency as a Natural resource of Medicine; Change its Status From Invasive to Useful plant. International Journal of Ecophysiology, 5(1), 1–4. https://doi.org/10.32734/ijoep.v5i1.11652
Marissa, M., Adittio, O., Leviyan, R., & Zahrawan, A. F. (2024). Hospital Management on Stunting Intervention For Economic Growth Acceleration Towards The Golden Indonesia 2045. In Proceeding of International Conference on Economic Issues (Vol. 1, No. 1, pp. 269-296). Retrieved from https://proceeding.unram.ac.id/index.php/icones/article/view/3336
Mawan, A. R., Indriwati, S. E., & Suhadi, S. (2018). Aktivitas Antibakteri Ekstrak Metanol Buah Syzygium polyanthum terhadap Pertumbuhan Bakteri Escherichia coli. Bioeksperimen: Jurnal Penelitian Biologi, 4(1), 64–68. https://doi.org/10.23917/bioeksperimen.v4i1.5934
Popović, N., Brdarić, E., Đokić, J., Dinić, M., Veljović, K., Golić, N., & Terzić-Vidojević, A. (2020). Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. Microorganisms, 8(10). https://doi.org/10.3390/microorganisms8101586
Riadi, S., Situmeang, S. M. ., & Musthari, M. (2017). Isolasi Dan Uji Aktivitas Antimikroba Bakteri Asam Laktat (Bal) Dari Yoghurt Dalam Menghambat Pertumbuhan Bakteri Escherichia coli DAN Salmonella typhi. Jurnal Biosains, 3(3), 144. https://doi.org/10.24114/jbio.v3i3.8302
Rinanda, T., Riani, C., Artarini, A., & Sasongko, L. (2023). Correlation between gut microbiota composition, enteric infections and linear growth impairment: a case–control study in childhood stunting in Pidie, Aceh, Indonesia. Gut Pathogens, 15(1), 1–14. https://doi.org/10.1186/s13099-023-00581-w
Shokryazdan, P., Sieo, C. C., Kalavathy, R., Liang, J. B., Alitheen, N. B., Faseleh Jahromi, M., & Ho, Y. W. (2014). Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. BioMed Research International, 2014, 927268. https://doi.org/10.1155/2014/927268
Sorensen, K., Atamer, Z., & Waite-Cusic, J. (2025). Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelflife. Journal of Dairy Science. https://doi.org/10.3168/jds.2024-25667
Windiyanti, R., Khotimah, S., & Zakiah, Z. (2023). Potensi Ekstrak Buah Jambu Tangkalak (Bellucia pentamera Naudin) sebagai Penghambat Pertumbuhan Escherichia coli ATCC 25922 dan Staphylococcus aureus. Life Scince, 12(1), 86–96. https://doi.org/10.15294/lifesci.v12i1.66777
Wirottama, I. W. A. A., Hartawan, I. N. B., Wati, D. K., & Mahalini, D. S. (2023). Characteristics of stunting in children in the Kintamani III Community Health Center area, Bangli District. Intisari Sains Medis, 14(3), 1353–1357. https://doi.org/10.15562/ism.v14i3.1911
Wulandari, G., Rahman, A. A., & Rubiyanti, R. (2019). Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill) Terhadap Bakteri Staphylococcus aureus. Media Informasi, 15(1), 74–80. https://doi.org/10.37160/bmi.v15i1.229
Zhang, Y., Tan, P., Zhao, Y., & Ma, X. (2022). Enterotoxigenic Escherichia coli: intestinal pathogenesis mechanisms and colonization resistance by gut microbiota. Gut Microbes, 14(1), 2055943. https://doi.org/10.1080/19490976.2022.2055943
Author Biographies
Shelly Festilia Agusanty, Poltekkes Kemenkes Pontianak
Maulidiyah Salim, Poltekkes Kemenkes Pontianak
Fara Chitra, Poltekkes Kemenkes Pontianak
License
Copyright (c) 2025 Shelly Festilia Agusanty, Maulidiyah Salim, Fara Chitra

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).