Safety Profile of Abelmoschus Manihot (L.) Medik Ethanol Leaf Extract: Acute Toxicity Study in Animal Models

Authors

Magfira B. Pantua , Viani Anggi , Indah Kurnia Utami , Yasinta Rakanita

DOI:

10.29303/jppipa.v10i9.9172

Published:

2024-09-30

Issue:

Vol. 10 No. 9 (2024): September

Keywords:

Abelmoschus manihot, Animal models, Safety profile, Toxicity

Research Articles

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How to Cite

Pantua, M. B., Anggi, V., Utami, I. K., & Rakanita, Y. (2024). Safety Profile of Abelmoschus Manihot (L.) Medik Ethanol Leaf Extract: Acute Toxicity Study in Animal Models. Jurnal Penelitian Pendidikan IPA, 10(9), 7122–7130. https://doi.org/10.29303/jppipa.v10i9.9172

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Abstract

Abelmoschus Manihot (L.) Medik, commonly known as Abelmoschus manihot, is a traditional medicinal plant from Palu, Central Sulawesi, Indonesia, widely utilized for its potential health benefits. This research aims to evaluate the acute toxicity of ethanol extract from Abelmoschus manihot leaves, focusing on determining the lethal dose (LD50) in rats. The study involved extracts being administered at a range of doses of 500 mg/kg, 1000 mg/ kg, and 2000 mg/Kg of body weight (BB) in a rat population. Rats were observed for 24 hours to look for signs of toxicity and death. The LD50 value, which determines the dose required to cause death in 50% of the test population, is calculated based on the results. This research provides insights into the safety profile of ethanol extract from Abelmoschus manihot leaves and contributes to understanding its potential risks when used in traditional medicine. No significant deaths were observed at lower doses, but higher doses resulted in observable toxic effects, indicating the need for careful dosing in therapeutic applications. The results show that although Abelmoschus Manihot did not exhibit acute toxic effects on rats at doses of 500 mg/kg, 1000 mg/kg, and 2000 mg/kg body weight. No significant deaths or severe toxic symptoms were observed during the 24-hour observation period up to the 14-day observation, and there were no differences in the body weight of the rats during the 14-day observation

References

Abdel-Razek, M. A. M., Abdelwahab, M. F., Abdelmohsen, U. R., & Hamed, A. N. E. (2023). A Review: Pharmacological Activity and Phytochemical Profile of Abelmoschus Esculentus (2010-2022. RSC Advances, 13(22), 15280–94. https://doi.org/10.1039/d3ra01367g.

Anggi, V. (2021). Total Flavonoid, Alkaloid and Tannin on Leaves and Stems of Abelmoschus Manihot L. Medik From Palu of Central Sulawesi. Journal of Bio Innovation, 10(1), 109–14. https://doi.org/10.46344/jbino.2021.v010i01.08.

Anggi, V., & Adikusuma, W. (2019). Total Antioxidant and In-Vitro Cytotoxic of Abelmoschus Manihot (L.) Medik from Palu of Central Sulawesi and Doxorubicin on 4t1 Cells Line and Vero Cells. Research Journal of Pharmacy and Technology, 12(11), 5472–76. https://doi.org/10.5958/0974-360X.2019.00949.1.

Anggi, V., & Masyita, A. A. (2022). Combination Effects of Abelmoschus manihot (L.) Medik of N-Hexane extracts and Doxorubicin in Breast cancer 4T1 Cells Line. Research Journal of Pharmacy and Technology, 15(2), 639–642. https://doi.org/10.52711/0974-360X.2022.00105

Arsyad, R., Amin, A., & Waris, R. (2023). Teknik Pembuatan Dan Nilai Rendamen Simplisia Dan Ekstrak Etanol Biji Bagore (Caesalpinia Crista L.) Asal Polewali Mandar. Makassar Natural Product Journal, 1(3), 2023–2138. Retrieved from https://journal.farmasi.umi.ac.id/index.php/mnpj.

BPOM. (2022). Panduan penyusunan protokol uji praklinik uji toksisitas akut. Direktural Registrasi Obat Tradisional, Suplemen Kesehatan Dan Kosmetik BPOM, 1–23. Retrieved from https://shorturl.asia/4NTFf

BPOM RI. (2022). Peraturan BPOM No 10 Tahun 2022 Pedoman Uji Toksisitas Praklinik Secara In Vivo. In Bpom Ri (Issue 490, pp. 1–16). Retrieved from https://shorturl.asia/jv4Me

Chang, C. C., Houng, J. Y., Peng, W. H., Yeh, T. W., Wang, Y. Y., Chen, Y. L., Chang, T. H., Hung, W. C., & Yu, T. H. (2022). Effects of Abelmoschus Manihot Flower Extract on Enhancing Sexual Arousal and Reproductive Performance in Zebrafish. Molecules, 27(7), 1–15. https://doi.org/10.3390/molecules27072218.

Chumbhale, D. S., & Khyade, M. S. (2022). Pharmacognostic Evaluation and Development of Quality Control Parameters for Root of Abelmoschus Manihot (L.) Medik. Pharmacognosy Research, 15(1), 101–11. https://doi.org/10.5530/097484900263.

Erhirhie, E. O., Ihekwereme, C. P., & Ilodigwe, E. E. (2018). Advances in Acute Toxicity Testing: Strengths, Weaknesses and Regulatory Acceptance. Interdisciplinary Toxicology, 11(1), 5–12. https://doi.org/10.2478/intox-2018-0001

Gao, Y., Liang, Z., Lv, N., Shan, J., Zhou, H., Zhang, J., & Shi, L. (2022). Exploring the Total Flavones of Abelmoschus Manihot against IAV-Induced Lung Inflammation by Network Pharmacology. BMC Complementary Medicine and Therapies, 22(1), 1–15. https://doi.org/10.1186/s12906-022-03509-0

Gurav, A. V. M., & Vithoba, R. (2016). Phytochemical and Nutritional Studies in the Genus Abelmoschus Medik. Intech, 1, 1–13. Retrieved from https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics.

Hou, J., Qian, J., Li, Z., Gong, A., Zhong, S., Qiao, L., & Qian, S. (2020). Bioactive Compounds from Abelmoschus Manihot l. Alleviate the Progression of Multiple Myeloma in Mouse Model and Improve Bone Marrow Microenvironment. OncoTargets and Therapy, 13, 959–73. https://doi.org/10.2147/OTT.S235944

Hou, J., Zhou, Y., Ran, L., Chen, Y., Zhang, T., Sun, B., Yang, Y., Sang, Q., & Cao, L. (2023). Transcriptome and Metabolome Analysis Reveal the Flavonoid Biosynthesis Mechanism of Abelmoschus Manihot L. BMC Complementary Medicine and Therapies. https://doi.org/10.3390/metabo13020216

Indrawati, E., & Setijorini, L. E. (2024). Prospects of Gedi Plant (Abelmoschus Manihot L.) as a Functional Food and Herbal Medicine. E3S Web of Conferences 483. https://doi.org/10.1051/e3sconf/202448302004

Kharchoufa, L., Bouhrim, M., Bencheikh, N., Assri, S. El, Amirou, A., Yamani, A., Choukri, M., Mekhfi, H., & Elachouri, M. (2020). Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon Scoparium Pomel (Hammada Scoparia (Pomel)) by Oral Administration in Rodents. BioMed Research International. https://doi.org/10.1155/2020/4020647.

Kim, H., Dusabimana, T., Kim, S. R., Je, J., Jeong, K., Kang, M. C., Cho, K. M., Kim, H. J., & Park, S. W. (2018). Supplementation of Abelmoschus Manihot Ameliorates Diabetic Nephropathy and Hepatic Steatosis by Activating Autophagy in Mice. Nutrients, 10(11), 1–16. https://doi.org/10.3390/nu10111703.

Li, J., Zhang, J., & Wang, M. (2016). Extraction of Flavonoids from the Flowers of Abelmoschus Manihot (l.) Medic by Modified Supercritical Co2 Extraction and Determination of Antioxidant and Anti-Adipogenic Activity. Molecules, 21(810), 1–14. https://doi.org/10.3390/molecules21070810

Li, M., Han, R., Li, J., Wu, W., & Gu, J. (2024). Research Progress in Acute Oral Toxicity Testing Methods. International Journal of Biology and Life Sciences, 6(1), 19–22. https://doi.org/10.54097/nv9van65

Liao, J. C., Li, C. Y., Teng, F. M., Jian-Chen, J. Y. Y., Ju, W. Z., & Zou, J. D. (2022). Integrated Analysis of Comprehensive Metabolomics and Network Pharmacology to Reveal the Mechanisms of Abelmoschus Manihot (L.) Medik. https://doi.org/10.3389/fphar.2022.1064498

Luan, F., Wu, Q., Yang, Y., Lv, H., Liu, D., Gan, Z., & Zeng, N. (2020). Traditional Uses, Chemical Constituents, Biological Properties, Clinical Settings, and Toxicities of Abelmoschus Manihot L.: A Comprehensive Review. Frontiers in Pharmacology, 11. https://doi.org/10.3389/fphar.2020.01068

Park, M. H., Yeom, Y. J., Ganbat, D., Kim, M. K., Kim, S. B., Lee, Y. J., & Lee, S. J. (2023). Fermentation of Abelmoschus Manihot Extract with Halophilic Bacillus Licheniformis CP6 Results in Enhanced Anti-Inflammatory Activities. Nutrients, 15(2). https://doi.org/10.3390/nu15020309

Patala, R., & Anggi, V. (2022). Pharmacophore Modeling and Molecular Docking of Flavonoid Derivatives in Abelmoschus Manihot Against Human Estrogen Receptor Alpha of Breast Cancer. Sciences of Pharmacy, 1(2), 1–9. https://doi.org/10.58920/sciphar01020001

Prasetiyo, A. (2023). The Inhibition of α-Glucosidase Enzyme Activity from Standardised Ethanol Extract of Abelmoschus Manihot (L.) Medik Leaves (Penghambatan Aktivitas Enzim α-Glukosidase Oleh Ekstrak Etanol Terstandar Daun Abelmoschus Manihot (L.) Medik. Jurnal Ilmu Kefarmasian Indonesia, 21(2), 159–64. https://doi.org/10.35814/jifi.v21i2.1387

Priyanka, G., & Elumalai, K. (2019). Evaluation of Acute Oral Toxicity Induced by Aqueous Extract of Mahavallathy Leghiyam in Rats. Drug Invention Today, 11(10), 2497–2501. Retrieved from https://shorturl.asia/Hw02S

Selvaraj, D., Subramanian, A., & Samuel, T. (2020). GC-MS Analysis of Abelmoschus Manihot (L.) Medik (Malvaceae) Leaves. World Journal of Advanced Research and Reviews, 5(2), 67–79. https://doi.org/10.30574/wjarr

Shi, R., Tao, Y., Tang, H., Wu, C., Fei, J., Ge, H., Gu, H. F., & Wu, J. (2023). Abelmoschus Manihot Ameliorates the Levels of Circulating Metabolites in Diabetic Nephropathy by Modulating Gut Microbiota in Non-Obese Diabetes Mice. Microbial Biotechnology, 16(4), 813–26. https://doi.org/10.1111/1751-7915.14200

Tandi, J., Roem, M., & Yuliet, Y. (2017). Efek Nefroprotektif Kombinasi Ekstrak Daun Gedi Merah Dan Daun Kumis Kucing Pada Tikus Induksi Etilen Glikol. Journal Of Tropical Pharmacy And Chemistry, 4(1), 27–34. https://doi.org/10.25026/jtpc.v4i1.129

Tangka, J., Barung, E. N., Lyrawati, D., Soeatmadji, D., & Nurdiana, N. (2022). DPP-IV Inhibitory Activity of the Ethanolic Extract of Red Gedi Leaves Abelmoschus Manihot L. Medic. Open Access Macedonian Journal of Medical Sciences, 10, 207–13. https://doi.org/10.3889/oamjms.2022.8356

Wang, S. W., Chang, C. C., Hsuan, C. F., Chang, T. H., Chen, Y. L., Wang, Y. Y., Yu, T. H., Wu, C. C., & Houng, J. Y. (2022). Neuroprotective Effect of Abelmoschus Manihot Flower Extracts against the H2O2-Induced Cytotoxicity, Oxidative Stress and Inflammation in PC12 Cells. Bioengineering, 9(10), 1–17. https://doi.org/10.3390/bioengineering9100596.

Winata, G. M., Hardinsyah, H., Marliyati, S. A., Rimbawan, R., & Andrianto, D. (2024). Phytochemical Compounds and Antioxidant Capacities of Abelmoschus Manihot Leaf Extracts Using Different Solvents. Biodiversitas, 25(3), 942–49. https://doi.org/10.13057/biodiv/d250305.

Wu, X., & Wang, F. (2023). Spectrum-Effect Relationship and Component Knock-Out or Knock- In in Total Flavones of Abelmoschus manihot. 1–17.

Wulan, O. T., & Indradi, R. B. (2018). Profik Fitokimia Dan Aktivitas Farmakologi Gedi (Abelmoschus Manihot (L.) Medik. Farmaka, 16(2), 202–9. Retrieved from https://garuda.kemdikbud.go.id/documents/detail/1452350

Xu, Z., Qian, L., Niu, R., Wang, Y., Yang, Y., Liu, C., & Lin, X. (2022). Mechanism of Abelmoschus manihot L. in the Treatment of Contrast-Induced Nephropathy on the Basis of Network Pharmacology Analysis. Frontiers in Nephrology, 2(April), 1–14. https://doi.org/10.3389/fneph.2022.834513

Zakari, A. (2018). In vivo Acute Toxicity (LD50) Studies and Phytochemical Screening of Stem Bark Extracts of Detarium microcarpum Guilt and per (Caesalpinioideae) and Myosortisscorpioides L. (boraginaceae). Current Trends in Biomedical Engineering & Biosciences, 14(4). https://doi.org/10.19080/ctbeb.2018.14.555891

Zhang, M., Wu, D., Xu, J., Liu, L., Jiao, W., Yu, J., & Chen, G. (2022). Suppression of NLRP3 Inflammasome by Dihydroarteannuin via the HIF‐1α and JAK3/STAT3 Signaling Pathway Contributes to Attenuation of Collagen-Induced Arthritis in Mice. Frontiers in Pharmacology, 13(April), 1–8. https://doi.org/10.3389/fphar.2022.884881

Author Biographies

Magfira B. Pantua, College of Pharmaceutical Sciences Pelita Mas of Central Sulawesi

Viani Anggi, College of Pharmaceutical Sciences Pelita Mas of Central Sulawesi

Indah Kurnia Utami, College of Pharmaceutical Sciences Pelita Mas of Central Sulawesi

Yasinta Rakanita, College of Pharmaceutical Sciences Pelita Mas of Central Sulawesi

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Copyright (c) 2024 Magfira B. Pantua, Viani Anggi, Indah Kurnia Utami, Yasinta Rakanita

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