Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology

Authors

Nadiah Nurandi , Sri Andayani , Yuni Kilawati

DOI:

10.29303/jppipa.v10i12.9338

Published:

2024-12-22

Issue:

Vol. 10 No. 12 (2024): December

Keywords:

Antibacterial, Fractionation, Histopathology, Momordica charantia

Research Articles

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How to Cite

Nurandi, N., Andayani, S., & Kilawati, Y. (2024). Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology. Jurnal Penelitian Pendidikan IPA, 10(12), 10284–10292. https://doi.org/10.29303/jppipa.v10i12.9338

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Abstract

Koi fish (Cyprinus carpio) is a type of freshwater ornamental fish with significant economic value, attracting widespread public interest due to its vibrant colors and agile movements. Therefore, this study introduces a novel approach by evaluating the antibacterial activity of bitter melon extract fraction (Momordica charantia) against A. salmonicida infection in koi fish and its impact on gill histopathology. The novelty of this study lies in the exploration of bitter melon extract fraction as a natural, eco-friendly antibacterial alternative to synthetic antibiotics in aquaculture. This research is crucial as it addresses the growing global concern over antibiotic resistance and the urgent need for sustainable, natural solutions in fish health management. Antibacterial tests were conducted to assess the ability of the bitter melon extract to inhibit bacterial growth, while histopathological analysis aimed to observe the specific changes in gill tissues of infected koi fish. The results revealed that the bitter melon extract fraction significantly reduced bacterial count and improved gill tissue integrity. Histopathological improvements were observed, including reduced hyperplasia, lamellar fusion, edema, and necrosis, indicating tissue recovery. This finding highlights the potential of bitter melon extract fraction as a natural antibacterial agent for controlling bacterial infections in fish, offering an alternative to chemical antibiotics. Further research is needed to determine the optimal dosage and to explore its antibacterial mechanism, ensuring its safe and effective application in aquaculture.

References

Akhter, S., Asna, S. M. Z. H., Rahman, M. M., & Hafez, M. A. (2012). Study of bacterial infection and antimicrobial sensitivity pattern in diabetic foot lesion. Bangladesh Journal of Medical Microbiology, 6(2), 20–23. https://doi.org/10.3329/bjmm.v6i2.19372

Aniputri, F. D., & Hutabarat, J. (2014). Pengaruh Ekstrak Bawang Putih (Allium Sativum) Terhadap Tingkat Pencegahan Infeksi Bakteri Aeromonas Hydrophila Dan Kelulushidupan Ikan Nila (Oreochromis Niloticus. Journal of Aquaculture Management and Technology, 3(2). Retrieved from http://ejournals1.undip.ac.id/index.php/jfpik

Apriliyanti, P., Sarjito, & Prayitno, S. . (2013). Identifikasi Bakteri Agensia Penyebab Motile Aeromonas Pada Ikan Lele Dumbo (Clarias Gariepinus) Identification Of Agents Bacteria Causing Motile Aeromonas On Dumbo Catfish (Clarias Gariepinus) From Rowosari Subdistrict Kendal District. Journal Of Aquaculture Management and Technology, 2(2), 1–9. Retrieved from http://ejournal-s1.undip.ac.id/Index.Php/Jfpik

Asfi, D., Yuliastuti Farmasi, R. K., & Farmasi Yamasi Makassar, A. (2023). Uji Daya Hambat Ekstrak Etanol Daun Miana Merah (Coleus Benth) Terhadap Staphylococcus Aureus. Jurnal Kesehatan Yamasi Makassar, 7(1), 10–16. Retrieved from http://scholar.unand.ac.id/id/eprint/470437

Bortolotti, M., Mercatelli, D., & Polito, L. (2019). Momordica Charantia, A Nutraceutical Approach For Inflammatory Related Diseases. Frontiers In Pharmacology, 10(May). https://doi.org/10.3389/Fphar.2019.00486

Chung, J. Y., Choo, J. H., Lee, M. H., & Hwang, J. K. (2006). Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine, 13(4), 261–266. https://doi.org/10.1016/j.phymed.2004.04.007

El-Sayed, W. M. M., Elshaer, M. M., Ibrahim, H. A. H., & El-Metwaly, M. E. A. (2020). Antimicrobial agents from sea urchin (Diadema setosum) collected from the redsea, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 24(5), 33–51. https://doi.org/10.21608/ejabf.2020.103181

Emilia, I., Arif Setiawan, A., Novianti, D., Mutiara, D., & Rangga, R. (2023). Skrining Fitokimia Ekstrak Daun Sungkai (Peronema Canescens Jack Secara Infundasi Dan Maserasi. Jurnal Indobiosains, 5(2). https://doi.org/10.31851/indobiosains.v5i2.12597

Gruber, A., Drabenstedt, A., Tietz, C., Fleury, L., Wrachtrup, J., & Borczyskowski, C. V. (1997). Scanning confocal optical microscopy and magnetic resonance on single defect centers. Science, 276(5321), 2012–2014. https://doi.org/10.1126/science.276.5321.2012

Hardani, H., Sukmana, D. J., Atfal, B., & Pertiwi, A. D. (2023). Potential antimicrobial ethyl acetate extracts of ur burst shells against S. aureus bacteria from diabetic foot wounds. Jurnal Penelitian Pendidikan IPA, 9(3), 1045–1049. https://doi.org/10.29303/jppipa.v9i3.288

Irshath, A. A., Rajan, A. P., Vimal, S., Prabhakaran, V. S., & Ganesan, R. (2023). Bacterial Pathogenesis In Various Fish Diseases: Recent Advances And Specific Challenges In Vaccine Development. Vaccines, 11(2). https://doi.org/10.3390/Vaccines11020470

Juwitaningsih, T., Sari, S. A., Jahro, I. S., & Windayani, N. (2021). Phytochemical Analysis And Antibacterial Activity Of Acetone Extract Of Secang (Caesalpinia Sappan L. Jurnal Jamu Indonesia, 6(2), 68–74. https://doi.org/10.29244/Jji.V6i2.209

Kamar, I., Zahara, F., Yuniharni, D., & Umairah, R. U. (2021). Identifikasi parasetamol dalam jamu pegal linu menggunakan metode kromatografi lapis tipis (KLT. QUIMICA: Jurnal Kimia Sains Dan Terapan, 3(1), 24–29. Retrieved from https://ejurnalunsam.id/index.php/JQ

Kumar, S., Yadav, M., Yadav, A., & Yadav, J. P. (2017). Impact of spatial and climatic conditions on phytochemical diversity and in vitro antioxidant activity of Indian Aloe vera (L. Burm. f. South African Journal of Botany, 111, 50–59. https://doi.org/10.1016/j.sajb.2017.03.012

Laoli, D., Susanti, N. M., Tillah, R., Telaumbanua, B. V., Zebua, R. D., Dawolo, J., Zebua, O., & Zega, A. (2024). Efektivitas Bahan Alami Sebagai Agen Antimikroba Dalam Pengobatan Penyakit Ikan Air Tawar: Tinjauan Literatur (Vol. 2, Issue 2, pp. 84–97). https://doi.org/10.62951/Zoologi.V2i2.66

Lê, H. V., Pham, L. V. B., Vo, B. N. H., Le, N. T. H., Nguyen, Q. H., Luu, P. V., Huynh, T. D., Nguyen, K. D., & Nguyen, N. H. (2024). Novel application of orange marigold flower petal (Tagetes erecta L.) as a dietary supplement to improve skin redness of Koi carps (Cyprinus carpio L.). VNUHCM Journal of Engineering and Technology, 7(1), press--press. Retrieved from https://stdjet.scienceandtechnology.com.vn/index.php/stdjet/article/view/1338

Maftuch, M., Setyawan, F. H., & Suprastyani, H. (2018). Uji daya hambat ekstrak Chaetoceros calcitrans terhadap bakteri Aeromonas salmonicida. JFMR-Journal of Fisheries and Marine Research, 2(1), 39–46. Retrieved from https://shorturl.asia/6qHon

Maleta, H. S., Indrawati, R., Limantara, L., & Brotosudarmo, T. H. P. (2018). Ragam metode ekstraksi karotenoid dari sumber tumbuhan dalam dekade terakhir (telaah literatur. Jurnal Rekayasa Kimia & Lingkungan, 13(1), 40–50. https://doi.org/10.23955/rkl.v13i1.10008

Muribeca, A. D. J. B., Gomes, P. W. P., Paes, S. S., Costa, A. P. A., Gomes, P. W. P., Viana, J. D. S., Reis, J. D. E., Pamplona, S. D. G. S. R., Silva, C., Bauermeister, A., Santos, L. D. S., & Silva, M. N. (2022). Antibacterial Activity From Momordica Charantia L. Leaves And Flavones Enriched Phase. Pharmaceutics, 14(9). https://doi.org/10.3390/Pharmaceutics14091796

Murwantoko, M., Rozi, R., Istiqomah, I., & Nitimulyo, K. H. (2013). Isolasi, Karakterisasi, dan Patogenitas Bakteri Penyebab Penyakit pada Gurami (Osphronemus goramy) di Kabupaten Bantul. Jurnal Perikanan Universitas Gadjah Mada, 15(2), 83–90. https://doi.org/10.22146/jfs.9102

Nurjannah, R. D. D., Prayitno, S. B., Sarjito, S., & Lusiastuti, A. M. (2013). Pengaruh ekstrak daun sirsak (Annona muricata) terhadap profil darah dan kelulushidupan ikan mas (Cyprinus carpio) yang diinfeksi bakteri Aeromonas hydrophila. Journal of Aquaculture Management and Technology, 2(4), 72–83. Retrieved from https://ejournal3.undip.ac.id/index.php/jamt/article/view/4808

Rini, C. S., Prakoso, Y. A., Amanatul Kholifah, D., & Ilmu, F. (2021). Uji Antibakteri Ekstrak Buah Pare (Momordica Charantia) Terhadap Bakteri Streptococcus Pneumoniae dan Klebsiella Pneumoniae. Prosiding Seminar Nasional Tahun 2021. Retrieved from https://jurnal.polbangtanyoma.ac.id/pros2021yoma/article/view/645

Singh, R., Iye, S., Prasad, S., Deshmukh, N., Gupta, U., Zanje, A., & Joshi, S. (2017). Phytochemical analysis of Muntingia calabura extracts possessing anti-microbial and anti-fouling activities. International Journal Pharmacogn Phytochem Res, 9(6), 826–832. https://doi.org/10.25258/phyto.v9i6.8186

Tr, T. A., Indriany, S., Sutriana, A., Rosmaidar, R., Asmilia, N., Panjaitan, B., & Hamdan, H. (2016). Uji Toksisitas Akut Ekstrak Etanol Daun Malaka (Phyllantus Emblica) Terhadap Mencit (Mus Musculus) (Acute Toxicity Test Of Ethanolic Extract Of Malaka (Phyllantus Emblica) Leaves On Mice (Mus Musculus). Jurnal Kedokteran Hewan-Indonesian Journal Of Veterinary Sciences, 10(2), 192–194. https://doi.org/10.21157/j.ked.hewan.v10i2.5137

Wardhani, A. K., & Kusdarwati, S. R. (2017). Gambaran histopatologi kulit dan insang benih ikan lele (Clarias sp.) yang terinfeksi saprolegnia sp. Dan yang telah diobati dengan ekstrak daun sirih (Piper betle L. Journal of Aquaculture and Fish Health, 7(1), 26–31. Retrieved from http://repository.unair.ac.id/id/eprint/123113

Zaini, W. S., & Shufiyani, S. (2017). Uji Daya Hambat Air Perasan Buah Pare (Momordica Charantia L.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Escherichia Coli Secara In Vitro. Jurnal Medikes, 4(2). Retrieved from https://jurnal.poltekkesbanten.ac.id/Medikes/article/download/81/64

Author Biographies

Nadiah Nurandi, University of Brawijaya

Sri Andayani, University of Brawijaya

Yuni Kilawati, University of Brawijaya

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Copyright (c) 2024 Nadiah Nurandi, Sri Andayani, Yuni Kilawati

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