Display of Free-range Chicken Semen After Supplementation of Phylanthus Niruri Herbal Extract and Kaffir Lime Leaf Extract (Citrus Hystrix D. C)
DOI:
10.29303/jppipa.v11i2.9748Published:
2025-02-25Issue:
Vol. 11 No. 2 (2025): FebruaryKeywords:
Free-range chicken, Kaffir lime leaf extract, Phylanthus niruri herbal extract, Semen displayResearch Articles
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Abstract
This study was conducted to examine the supplementation of green meniran herb extract (Phylanthus niruri L) and kaffir lime leaf extract (Cytrus hystrix D. C.) on the appearance of free-range chicken semen. The study was conducted using a completely random design of unidirectional patterns, using 24 male free-range chickens aged about one year with an average weight of 1.8 kg with eight treatments, three replicates each. Each replicate consisted of 1 chicken. Basal feed uses BR-1 chicken feed produced by PT. Japfa Comfeed Indonesia Tbk. After 1 week of basal feeding, chicken sperm was taken and tested for control (H-0), then began to be treated with testosterone supplementation, as a positive control, meniran extract, kaffir lime leaf extract, or a mixture thereof. The following semen collection is carried out after 1 week of treatment. Semen collection is carried out every 3 days with five intakes. This study concludes that the supplementation of green meniran herb extract and kaffir lime leaf extract or a combination thereof, macroscopic, does not change the appearance of free-range chicken semen, both volume, color, consistent odor, and pH of semen, but microscopic significantly increase sperm concentration, motility, and Viability. There was no synergy between green meniran herb extract and kaffir lime leaf extract on the appearance of chicken semen.
References
Adeoye, G. O., Oleforuh-Okoleh, V. U., & Chukwuemeka, U. M. (2018). Influence of breed type and age on spermatological traits of Nigerian local chickens. Agro-Science, 16(1), 11. https://doi.org/10.4314/as.v16i1.3
Ahsan, F. (2020). Nutritional and Medicinal Properties of Stevia Rebaudiana. Current Research in Diabetes & Obesity Journal, 13(4). https://doi.org/10.19080/crdoj.2020.13.555867
Ansari, M., Zhandi, M., Kohram, H., Zaghari, M., Sadeghi, M., Gholami, M., H. Deldar, M., Fiore, M. Di, & Benson, A. P. (2018). D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology. Reproduction, Fertility and Development, 30(7), 1038-1048. https://doi.org/10.1071/RD17072
Arini, L. A. (2021). The study of sex steroid hormone compound in green algae (Chlorophyta) for female fertility: A literature review. IOP Conference Series: Earth and Environmental Science, 913(1), 012085. https://doi.org/10.1088/1755-1315/913/1/012085
Assersohn, K., Brekke, P., & Hemmings, N. (2021). Physiological factors influencing female fertility in birds. Royal Society Open Science, 8(7), 202274. https://doi.org/10.1098/rsos.202274
Awad, A. M., Shaikh, S. M. R., Jalab, R., Gulied, M. H., Nasser, M. S., Benamor, A., & Adham, S. (2019). Adsorption of organic pollutants by natural and modified clays: A comprehensive review. Separation and Purification Technology, 228, 115719. https://doi.org/10.1016/j.seppur.2019.115719
Aziman, M. A., Winarni, T. I., & Miranti, I. P. (2015). Pengaruh Paparan Insektisida Pada Testis Tikus Sprague Dawley: Kajian Risiko Keganasan Sel Germinal (Vol. 4, Issue 4). Retrieved from http://eprints.undip.ac.id/46190/9/Milzam_Auzan_Aziman_22010111140162_Lap.KTI_Bab8.pdf
Azizah, N., Komarudin, K., Pratiwi, N., Kostaman, T., & Sartika, T. (2023). Analisis Kualitas Semen Ayam Lokal Indonesia Berdasarkan Galur dan Umur Dewasa Kelamin yang Berbeda. Jurnal Agripet, 23(1), 40–45. https://doi.org/10.17969/agripet.v23i1.25747
Bebas, W., & Laksmi, D. N. D. I. (2013). Konsentrasi Spermatozoa Dan Motilitas Spermatozoa Ayam Hutan Hijau (Gallus varius). Buletin Veteriner Udayana, 5(1), 57–62. Retrieved from https://scholar.archive.org/work/mdxxfhremvfvvg2so7fqiiuccm/access/wayback/https://ojs.unud.ac.id/index.php/buletinvet/article/download/5740/4346
Bindari, Y. R., Shrestha, S., Gaire, Shrestha, N., & Nath, T. (2013). Effects of nutrition on reproduction- A review. Pelagia Research Library, 4(1), 421–429. Retrieved from https://www.primescholars.com/articles/effects-of-nutrition-on-reproduction-a-review.pdf
Cheah, Y., & Yang, W. (2011). Functions of essential nutrition for high quality spermatogenesis. Advances in Bioscience and Biotechnology, 02(04), 182–197. https://doi.org/10.4236/abb.2011.24029
Cohen, K., & Weinstein, A. M. (2018). Synthetic and Non-synthetic Cannabinoid Drugs and Their Adverse Effects-A Review From Public Health Prospective. Frontiers in Public Health, 6. https://doi.org/10.3389/fpubh.2018.00162
Danang, D. R., Isnaini, N., & Trisunuwati, P. (2012). Pengaruh Lama Simpan Semen Terhadap Kualitas Spermatozoa Ayam Kampung Dalam Pengencer Ringer’S Pada Suhu 4 C. TERNAK TROPIKA Journal of Tropical Animal Production, 13(1), 47–57. Retrieved from https://ternaktropika.ub.ac.id/index.php/tropika/article/view/163
Dawood, M. A. O., Koshio, S., & Esteban, M. Á. (2018). Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Reviews in Aquaculture, 10(4), 950–974. https://doi.org/10.1111/raq.12209
Desta, T. T. (2021). Indigenous village chicken production: a tool for poverty alleviation, the empowerment of women, and rural development. Tropical Animal Health and Production, 53(1), 1. https://doi.org/10.1007/s11250-020-02433-0
Djermanovic, V., Mitrovic, S., & Djekic, V. (2013). Rooster body weight influence on the reproductive performance of the broiler parents. Biotechnology in Animal Husbandry, 29(1), 83–91. https://doi.org/10.2298/bah1301083d
Enema, O. J., Umoh, U. F., Umoh, R. A., Ekpo, E. G., Adesina, S. K., & Eseyin, O. A. (2018). Chemistry and Pharmacology of Aphrodisiac Plants: A Review. Available Online Www.Jocpr.Com Journal of Chemical and Pharmaceutical Research, 10(7), 70–98. Retrieved from https://shorturl.asia/MDpuI
Ferlinahayati, F. (2001). Isolasi Steroid dari Daun Puteri Malu (Mimosa Invisi) dengan Pelarut N-Heksana. Jurnal Penelitian Sains, 10, 50–57. https://doi.org/10.56064/jps.v0i10.351
Garner, D. L., & Hafez, E. S. E. (2000). Spermatozoa and Seminal Plasma. Reproduction in Farm Animals, 96–109. https://doi.org/10.1002/9781119265306.ch7
Getachew, T. (2016). A Review Article of Artificial Insemination in Poultry. World s Veterinary Journal, 6(1), 25. https://doi.org/10.5455/wvj.20160263
Hambu, E. K., Arifiantini, R. I., Purwantara, B., & Darwati, S. (2016). Raw Semen Characteristics of Three Different Indonesian Local Roosters. Animal Production, 18(3), 165. https://doi.org/10.20884/1.jap.2016.18.3.555
Harmusyanto, R. (2013). Studi mengenai efek daun katuk (Sauropus androgynus (L) Merr.) terhadap libido kelinci jantan (Oryctolagus cuniculus) sebagai afrodisiak. Calyptra. Retrieved from https://journal.ubaya.ac.id/index.php/jimus/article/download/170/147
Haryuni, N., Lestariningsih, L., & Khopsoh, B. (2022). Perbaikan Kualitas Semen Ayam Kampung Melalui Peningkatan Energi Metabolisme Pakan. Journal of Science Nusantara, 2(3), 123–129. https://doi.org/10.28926/jsnu.v2i3.548
Hendarwati, M. (2014). Efek Antibakteri Ekstrak Daun Jeruk Purut (Citrus hystrix) Terhadap Bakteri Streptococcus mutans (in vitro). In Skripsi. Retrieved from https://www.semanticscholar.org/paper/EFEK-ANTIBAKTERI-EKSTRAK-DAUN-JERUK-PURUT-(Citrus-Miftahendarwati/a2efd72b2c634e22b48d0b65fd69d78e1ee0c403
Herbie, T. (2015). Tumbuhan Obat Untuk Penyembuhan Penyakit dan Kebugaran Tubuh. Yogyakarta: CV. Solusi Distribusi.
Hidayat C, & Sopiyana S. (2010). Potensi ayam sentul sebagai plasma nutfah asli ciamis jawa barat. Wartazoa, 20(4), 190–205. Retrieved from https://shorturl.asia/3hys4
Hoesni, F. (2016). Pengaruh Penggunaan Tris Dalam Pengencer Susu Skim Terhadap Resistensi Spermatozoa Sapi Simmental Pasca Pembekuan. Jurnal Ilmu-Ilmu Peternakan, 19(2), 77–82. https://doi.org/10.22437/jiiip.v19i2.3850
Huda, A. N., Mashudi, M., Kuswati, K., Susilawati, T., Wahjuningsih, S., Isnaini, N., Yekti, A. P. A., & Satria, A. T. (2018). Evaluasi Kecukupan Nutrisi Induk Sapi Potong di Desa Leran Wetan dan Leran Kulon, Kecamatan Palang, Kabupaten Tuban. TERNAK TROPIKA Journal of Tropical Animal Production, 19(2), 111–119. https://doi.org/10.21776/ub.jtapro.2018.019.02.6
Ismaya. (2014). Bioteknologi Inseminasi Buatan pada Sapi dan Kerbau. Yogyakarta: Gadjah Mada University Press.
Isnaeni, M., Faidiban, O. R., & Tethool, A. N. (2019). Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam). Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 9(2), 44. https://doi.org/10.30862/jipvet.v9i2.65
Junaedi, J., Arifiantini, R. I., Sumantri, C., & Gunawan, A. (2016). Use of glycerol as cryoprotectants in freezing Sentul chicken semen. Chalaza Journal of Animal Husbandry, 1(2), 6-13. https://doi.org/10.31327/chalaza.v1i2.165
Kartasudjana. (2001). Teknik Inseminasi Buatan Pada Ternak. Jurnal Agroland, 16(2). Retrieved from https://shorturl.asia/6JciN
Komarraju, A. K., Batchu, V. V. S. C., & Mandala, V. (2024). The Role of Artificial Intelligence and Machine Learning in Financial Processes: Innovations and Impacts on Accounts Payable, Accounts Receivable, and General Ledger. Nanotechnology Perceptions, 20(S8). https://doi.org/10.62441/nano-ntp.v20iS8.72
Komarudin, K., Sartika, T., Kostaman, T., Sopiyana, S., & Zainal, H. (2020). Analisis Bobot Badan dan Karakteristik Semen Ayam Gaok Terseleksi Generasi ke-6. Jurnal Agripet, 20(2). https://doi.org/10.17969/agripet.v20i2.16650
Kurniawati, F., Zaenab, S., & Wahyuni, S. (2015). Analisis perbandingan bentuk jaringan pembuluh trakea pada preparat maserasi berbagai genus piper sebagai sumber belajar biologi. JPBI (Jurnal Pendidikan Biologi Indonesia), 1(2). https://doi.org/10.22219/jpbi.v1i2.3326
Kusumawati, E. D., Woli, S. L., Krisnaningsih, A. T. N., Susanto, W. E., & Rahadi, S. (2018). Motilitas dan Vabilitas Spermatozoa Ayam Kampung pada Suhu 5°C Menggunakan Pengencer dan Lama Simpan yang Berbeda. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 5(2), 102. https://doi.org/10.33772/jitro.v5i3.7242
Leeson, & Steven. (2009). Broiler Breeder Production. Broiler Breeder Production..pdf. Nottingham University Press.
Lubis, T. M. (2011). Motilitas Spermatozoa Ayam Kampung dalam Pengencer Air Kelapa, NaCl Fisiologis dan Air Kelapa-NaCl Fisiologis pada 25-29°C. Jurnal Agripet, 11(2), 45–50. https://doi.org/10.17969/agripet.v11i2.374
Mau, Y., Dethan, A. A., & Lisnahan, C. V. (2022). Kualitas Mikroskopis Semen Ayam Kampung yang Disumplementasikan L-Arginine dan L-Lysine HCl Dalam Pakan. Jas, 7(2), 23–26. https://doi.org/10.32938/ja.v7i2.1691
Nangoy, F. J., & Karisoh, L. C. H. (2018). Pemberdayaan Masyarakat Pedesaan pada Ayam Kampung Pasawungen di Desa Pahaleten Kecamatan Kakas Kabupaten Minahasa Provinsi Sulawesi Utara. Jurnal Lppm Bidang Sains Dan Teknologi, 5(2), 57–66. https://doi.org/10.35801/jlppmsains.5.2.2018.24091
Nasrudin. (2017). Isolasi Senyawa Steroid Dari Kukit Akar Senggugu (Clerodendrum Serratum L.Moon). PHARMACON :Journal Ilmiah Farmasi - UNSRAT, 6(3). https://doi.org/10.35799/pha.6.2017.17119
Nataamijaya, A. ., & Setioko, A. . (2002). Koleksi Ayam Lokal secara ex situ dengan Memanfaatkan Informasi Bioteknis Kondisi in situ. Retrieved from https://shorturl.asia/QkUS1
Owaba, A. D., Etim, E. I., Johnson, E. C., & Umoh, U. F. (2021). Aphrodisiac agents used in traditional medicine and their mechanism of action - A Review. Journal of Pharmacognosy and Phytochemistry, 10(3), 126–153. https://doi.org/10.22271/phyto.2021.v10.i3b.14085
Pliego, A. B., Tavakoli, M., Khusro, A., Seidavi, A., Elghandour, M. M. M. Y., Salem, A. Z. M., Márquez-Molina, O., & Rene Rivas-Caceres, R. (2022). Beneficial and adverse effects of medicinal plants as feed supplements in poultry nutrition: a review. Animal Biotechnology, 33(2), 369–391. https://doi.org/10.1080/10495398.2020.1798973
Prache, S., Lebret, B., Baéza, E., Martin, B., Gautron, J., Feidt, C., Médale, F., Corraze, G., Raulet, M., Lefèvre, F., Verrez-Bagnis, V., & Sans, P. (2022). Review: Quality and authentication of organic animal products in Europe. Animal, 16, 100405. https://doi.org/10.1016/j.animal.2021.100405
Putranto, H. D., Nurmeiliasari, N., & Harferry, K. T. (2020). Studi Kualitas Semen Ayam Burgo. Buletin Peternakan Tropis, 1(1), 10–15. https://doi.org/10.31186/bpt.1.1.10-15
Rivai, H., Septika, R., & Boestari, A. (2013). Karakterisasi Ekstrak Herba Meniran ( Phyllanthus niruri Linn ). Jurnal Farmasi Higea, 5(2), 15–23.
Romero-Sanchez, H., Plumstead, P. W., Leksrisompong, N., Brannan, K. E., & Brake, J. (2008). Feeding broiler breeder males. 4. Deficient feed allocation reduces fertility and broiler progeny body weight. Poultry Science, 87(4), 805–811. https://doi.org/10.3382/ps.2007-00285
Sarkar, P. K. (2020). Motility, Viability and fertilizing ability of avian sperm stored under in vitro conditions. Reviews in Agricultural Science, 8, 15–27. https://doi.org/10.7831/ras.8.0_15
Sopiyana, S., Iskandar, S., Susanti, T., & Yogaswara, D. (2006). Pengaruh krioprotektan DMA, DMF dan Glycerol pada proses pembekuan semen ayam kampung. Seminar Nasional Teknologi Peternakan Dan Veteriner 2006, 702–708. Retrieved from https://shorturl.asia/vjVis
Suripta, H., & Astuti, P. (2021). Peningkatan Produksi Semen Ayam Kampung Melalui Suplementasi Daun Kelor (Moringa oleifera). AGRISAINTIFIKA: Jurnal Ilmu-Ilmu Pertanian, 5(2), 194. https://doi.org/10.32585/ags.v5i2.1986
Suripta, H., Astuti, P., & Nurtanti, I. (2023). Evaluation of Semen Quality of Free-Range Chickens After Addition of Putri Malu Leaf Herbal Extract (Mimosa pudica Linn). Jurnal Penelitian Pendidikan IPA, 9(9), 7383–7389. https://doi.org/10.29303/jppipa.v9i9.4930
Susilawati, T. (2013). Pedoman Inseminasi Buatan Pada Ternak. Universitas Brawijaya Press, 01, 1–7.
Taddesse, D., Wolde, D. T., & Tamir, B. (2016). Organic Livestock Farming and the Scenario in the Developing Countries: Opportunities and Challenges. Global Veterinaria, 16(4), 399–412. https://doi.org/10.5829/idosi.gv.2016.16.04.10368
Toyin, Y. M., Olakunle, A. T., & Adewunmi, A. M. (2014). Toxicity and Beneficial Effects of Some African Plants on the Reproductive System. In Toxicological Survey of African Medicinal Plants (pp. 445–492). Elsevier. https://doi.org/10.1016/B978-0-12-800018-2.00015-7
Yang, Y., Zhang, Y., Ding, J., Ai, S., Guo, R., Bai, X., & Yang, W. (2019). Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus. Avian Research, 10(1). https://doi.org/10.1186/s40657-019-0174-5
Yendraliza. (2013). Pengaruh nutrisi dalam pengelolaan reproduksi ternak (studi literatur). Kutubkhanah, 16(1), 20–26. Retrieved from http://ejournal.uin-suska.ac.id/index.php/Kutubkhanah/article/view/230
Yuwanta, T. (2016). Dasar Ternak Unggas. Journal of Chemical Information and Modeling, 53(9), 1689–1699. Retrieved from http://belajar.ditpsmk.net/wp-content/uploads/2014/09/Reproduksi-Hewan-1.pdf
Author Biographies
Heru Suripta, Universitas Muhammadiyah Karanganyar
Puji Astuti, Universitas Muhammadiyah Karanganyar
Ina Nurtanti, Universitas Muhammadiyah Karanganyar
Dimas Fajar Nugroho, Universitas Muhammadiyah Karanganyar
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