The Effect of Dietary Probiotic and Oxygen Supply on the Growth and Survival Rate of Eels (Anguilla bicollor)

Authors

Gagan Garnawansah , Dian Permana , Dasep Hasbullah , Zaenal Arifin Siregar , Dzikri Wahyudi , Boyun Handoyo , Indrian Rizka Amalia , Ratna Indria Sari

DOI:

10.29303/jppipa.v11i2.9998

Published:

2025-02-25

Issue:

Vol. 11 No. 2 (2025): February

Keywords:

Eel farming, Feed efficiency, Paddlewheel aeration, Probiotics, Sustainable aquaculture

Research Articles

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Garnawansah, G., Permana, D., Hasbullah, D., Siregar, Z. A., Wahyudi, D., Handoyo, B., … Sari, R. I. (2025). The Effect of Dietary Probiotic and Oxygen Supply on the Growth and Survival Rate of Eels (Anguilla bicollor). Jurnal Penelitian Pendidikan IPA, 11(2), 822–836. https://doi.org/10.29303/jppipa.v11i2.9998

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Abstract

The aquaculture of Anguilla bicollor has significant economic potential, but persistent challenges continue to hinder profitability for farmers. Two of the most pressing issues are low dissolved oxygen levels and poor feed conversion efficiency. These problems slow eel growth and drive-up operational costs, creating a bottleneck for sustainable production. This study examines a combined strategy to tackle these challenges by integrating probiotics (Lactobacillus sp.) and paddlewheel aeration. Research was conducted at BLUPPB Karawang, using 600 juvenile eels in ponds subjected to four distinct treatments: (1) probiotics and paddlewheels together, (2) paddlewheels-only, (3) probiotics-only, and (4) a control group with no intervention. Over a 45-day period, survival rates (SR), relative growth rates (RGR), and feed conversion ratios (FCR) were carefully monitored. The findings were promising. Ponds that used both probiotics and paddlewheels achieved the best results: an RGR of 1.19%, an SR of 100%, and an FCR of 1.09. Probiotics contributed to better gut health and digestion, while the paddlewheels boosted water oxygenation—creating an ideal growth environment. While no direct interaction between the two methods was observed, their combined application proved highly effective in improving productivity and sustainability. This integrated approach offers a practical solution to some of the most significant challenges in Anguilla bicollor aquaculture.

References

Ahn, H., Yamada, Y., Okamura, A., Horie, N., Mikawa, N., Tanaka, S., & Tsukamoto, K. (2012). Effect of water temperature on embryonic development and hatching time of the Japanese eel Anguilla japonica. Aquaculture, 330–333, 100–105. https://doi.org/10.1016/j.aquaculture.2011.12.020

Al-Shorbagy, W. M., Al-Shal, M. S., & Hasan, A. S. (2024). Improvement of the Aquatic Environment for the Nile Tilapia (Oreochromis niloticus) via a New Developed Automatic Feeding System. Egyptian Journal of Aquatic Biology & Fisheries, 28(6), 547–555. Retrieved from www.ejabf.journals.ekb.eg

Amrullah, Wahidah, Ardiansyah, Hartinah, Khatimah, K., Rosyida, E., & Novita, H. (2024). Feed supplementation with quorum quenching probiotics improved growth response, immune response, and resistance in the giant mottled eel, Anguilla marmorata. Egyptian Journal of Aquatic Research. https://doi.org/10.1016/j.ejar.2024.08.003

Anand, P. S. S., Kohli, M. P. S., Kumar, S., Sundaray, J. K., Roy, S. D., Venkateshwarlu, G., Sinha, A., & Pailan, G. H. (2014). Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon. Aquaculture, 418–419, 108–115. https://doi.org/10.1016/j.aquaculture.2013.09.051

Arai, T. (2016). Biology and Ecology of Anguillid Eels. CRC Press Taylor & Francis Group.

Bossier, P., & Ekasari, J. (2017). Biofloc technology application in aquaculture to support sustainable development goals. Microbial Biotechnology, 10(5), 1012–1016. https://doi.org/10.1111/1751-7915.12836

Brett, J. R., Hoar, W. S., & Randall, D. J. (1979). Fish Physiology: Bioenergetics and Growth. Academic Press.

Budiardi, T., Effendi, I., Rahman, M. A., & Vinasyiam, A. (2022). Production performance of nursery graded eel Anguilla bicolor bicolor in recirculating aquaculture system Kinerja produksi pendederan ikan sidat Anguilla bicolor bicolor hasil grading pada sistem resirkulasi. Jurnal Akuakultur Indonesia, 21(2), 109–117. https://doi.org/10.19027/jai.21.2.109-117

Capaldo, A., Gay, F., Caputo, I., Lionetti, L., Paolella, G., Di Gregorio, I., Martucciello, S., Di Lorenzo, M., Rosati, L., & Laforgia, V. (2021). Effects of environmental cocaine concentrations on COX and caspase-3 activity, GRP-78, ALT, CRP and blood glucose levels in the liver and kidney of the European eel (Anguilla anguilla). Ecotoxicology and Environmental Safety, 208. https://doi.org/10.1016/j.ecoenv.2020.111475

Chen, Y. H., Lee, W. C., Chen, C. C., Chen, Y. H., & Chiu Liao, I. (2006). Impact of externality on the optimal production of eel (Anguilla japonica) aquaculture in Taiwan. Aquaculture, 257(1–4), 18–29. https://doi.org/10.1016/j.aquaculture.2006.03.004

Chethurajupalli, L., & Tambireddy, N. (2022). Rearing of White Leg Shrimp Litopenaeus vannamei (Boone, 1931) in Biofloc and Substrate Systems: Microbial Community of Water, Growth and Immune Response of Shrimp. Turkish Journal of Fisheries and Aquatic Sciences, 22(3). https://doi.org/10.4194/TRJFAS20130

Cibuntu, S., Regency, B., Akbar, M. T., Chilmawati, D., & Wijayanto, D. (2023). The use of artificial feed, Tubifex sp. and their combination on growth and survival of eel (Anguilla bicolor bicolor) reared in (Vol. 16). Retrieved from http://www.bioflux.com.ro/aacl

Conforto, E., Vílchez-Gómez, L., Parrinello, D., Parisi, M. G., Esteban, M. Á., Cammarata, M., & Guardiola, F. A. (2021). Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae. Fish and Shellfish Immunology, 114, 330–339. https://doi.org/10.1016/j.fsi.2021.05.011

Cruz-Guerrero, A., Hernández-Sánchez, H., Rodríguez-Serrano, G., Gómez-Ruiz, L., García-Garibay, M., & Figueroa-González, I. (2014). Commercial probiotic bacteria and prebiotic carbohydrates: A fundamental study on prebiotics uptake, antimicrobials production and inhibition of pathogens. Journal of the Science of Food and Agriculture, 94(11), 2246–2252. https://doi.org/10.1002/jsfa.6549

De Schrijver, R., & Ollevier, F. (2000). Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus. Aquaculture, 186(1–2), 107–116. https://doi.org/10.1016/S0044-8486(99)00372-5

Degani, G., Horowitz, A., & Levanon, D. (1985). Effects Of Dietary Carbohydrate Source on Growth And Body Composition Of The European Eel (Anguilla anguilla). Aquaculture, 52(2), 97-104. https://doi.org/10.1016/0044-8486(86)90029-3

Dewanti, A. R., Putri, A. O., Istiqomah, I., & Isnansetyo, A. (2022). Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor). Jurnal Ilmiah Perikanan Dan Kelautan, 14(2), 203–213. https://doi.org/10.20473/jipk.v14i2.34315

Ellis, A. G., & Smith, D. G. (1983). Edema formation and impaired O2 transfer in ringer‐perfused gills of the eel, Anguilla australis. Journal of Experimental Zoology, 227(3), 371-380. https://doi.org/10.1002/jez.1402270306

Elmi, A., Casalini, A., Bertocchi, M., Emmanuele, P., Aniballi, C., Parmeggiani, A., Govoni, N., Ventrella, D., Mordenti, O., & Bacci, M. L. (2023). Comparative evaluation of the effects of different activating media and temperatures on European eel (Anguilla anguilla) sperm motility assessed by computer assisted sperm analysis. Research in Veterinary Science, 164. https://doi.org/10.1016/j.rvsc.2023.105045

Engin, K., & Carter, C. G. (2001). Ammonia and urea excretion rates of juvenile ž Australian short-finned eel Anguilla australis/ australis as influenced by dietary protein level. In Aquaculture (Vol. 194). https://doi.org/10.1016/S0044-8486(00)00506-8

Espírito-Santo, C., Guardiola, F. A., Ozório, R. O. A., & Magnoni, L. J. (2024). Short-term induced swimming activity enhanced innate immune parameters and antioxidant status of European eel (Anguilla anguilla). Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, 296. https://doi.org/10.1016/j.cbpa.2024.111680

Esteve, C., Merchán, R., & Alcaide, E. (2017). An outbreak of Shewanella putrefaciens group in wild eels Anguilla anguilla L. favoured by hypoxic aquatic environments. Journal of Fish Diseases, 40(7), 929–939. https://doi.org/10.1111/jfd.12574

Fatmawati, Fauzana, N. A., & Aminah. (2023). Utilization of Kelakai Wetland Plants (Stennochlaena palutris) and Different Doses of Probiotics in Feed Formulations on the Growth of Climbing Perch (Anabas testudineus Bloch). Jurnal Penelitian Pendidikan IPA, 9(8), 6619–6625. https://doi.org/10.29303/jppipa.v9i8.3570

Fauzana, N. A., Redha, E., & Aminah. (2023). Hematological Response Studies of Climbing Perch (Anabas testudineus) Fed with Fermented Feed on the Fermentation of Kelakai Leaves (Stenochlaena palustris). Jurnal Penelitian Pendidikan IPA, 9(10), 7879–7887. https://doi.org/10.29303/jppipa.v9i10.3568

Fujii, T., Yoshikawa, M., Kondo, N., Yamakawa, S., Funasaka, K., Hirooka, Y., & Tochio, T. (2024). Synbiotic administration in Japanese eels with prebiotic 1-kestose and probiotic Lactiplantibacillus plantarum FM8 improved feed efficiency and significantly reduced the levels of Edwardsiella. Fisheries Science, 90(1), 115–122. https://doi.org/10.1007/s12562-023-01739-w

Fukuda, N., Kuroki, M., Shinoda, A., Yamada, Y., Okamura, A., Aoyama, J., & Tsukamoto, K. (2009). Influence of water temperature and feeding regime on otolith growth in Anguilla japonica glass eels and elvers: Does otolith growth cease at low temperatures? Journal of Fish Biology, 74(9), 1915–1933. https://doi.org/10.1111/j.1095-8649.2009.02287.x

Fuller, R., & Fuller, R. (1992). History and development of probiotics. Probiotics: The scientific basis, 1-8. https://doi.org/10.1007/978-94-011-2364-8_1

Furuita, H., Hori, K., Suzuki, Sugita, T., & Yamamoto, T. (2007). Effect of n-3 and n-6 fatty acids in broodstock diet on reproduction and fatty acid composition of broodstock and eggs in the Japanese eel Anguilla japonica. Aquaculture, 267(1–4), 55–61. https://doi.org/10.1016/j.aquaculture.2007.01.039

Garnawansah, G., Suryaningtyas, E. W., & Khumaidi, A. (2017). Peningkatan produktivitas ikan sidat (anguila sp.) Dengan sistem polikultur. Samakia: Jurnal Ilmu Perikanan, 8(1), 44-49. Retrieved from https://fd.ibrahimy.ac.id/files/journals/1/articles/108/submission/proof/108-4-132-1-10-20170902.pdf

Gómez-Limia, L., Cobas, N., & Martínez, S. (2021). Proximate composition, fatty acid profile and total amino acid contents in samples of the European eel (Anguilla anguilla) of different weights. International Journal of Gastronomy and Food Science, 25. https://doi.org/10.1016/j.ijgfs.2021.100364

Grandi, G., Colombo, G., & Chicca, M. (2003). Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation. General and Comparative Endocrinology, 131(1), 66–76. https://doi.org/10.1016/S0016-6480(02)00646-9

Guo, S., Wan, Q., Xu, M., Chen, M., & Chen, Z. (2024). Transcriptome analysis of host anti-Aeromonas hydrophila infection revealed the pathogenicity of A. hydrophila to American eels (Anguilla rostrata). Fish and Shellfish Immunology, 148. https://doi.org/10.1016/j.fsi.2024.109504

Guo, Y., Chen, H., Tang, Y., Zhang, H., Jiang, B., Chen, S., Mao, L., Chen, C., Zhong, W., Wen, S., Mai, Y., & Cai, J. (2025). The effects of Lactobacillus plantarum and oxolinic acid on growth, immune-related genes, and gut bacterial community structures in juvenile Japanese eel (Anguilla Japonica). Aquaculture, 594. https://doi.org/10.1016/j.aquaculture.2024.741382

Haenen, O. L. M., Lehmann, J., Engelsma, M. Y., Stürenberg, F. J., Roozenburg, I., Kerkhoff, S., & Breteler, J. K. (2010). The health status of European silver eels, Anguilla anguilla, in the Dutch River Rhine Watershed and Lake IJsselmeer. Aquaculture, 309(1–4), 15–24. https://doi.org/10.1016/j.aquaculture.2010.08.026

Harun, H., Abdan, M., Suprihadi, S., Saputra, F., Kharunnisak, K., Samsuli, S., & Sulni, R. (2023). Application of herbal probiotics in feed on growth and blood profile of elver eels (Anguilla bicolor McClelland, 1844). Depik, 12(2), 152–158. https://doi.org/10.13170/depik.12.2.29105

Hatakeyama, R., Sudo, R., Higuchi, M., Satomi, M., Yatabe, T., Takasaki, R., Imaizumi, H., & Kazeto, Y. (2023). Individual variation in post-metamorphic changes in feeding incidence, digestive organ tissues and enzyme gene expression in Japanese eel Anguilla japonica glass eels. Aquaculture, 576. https://doi.org/10.1016/j.aquaculture.2023.739890

He, W., Wu, L., Li, S., & Guo, S. (2021). Transcriptome RNA-seq revealed lncRNAs activated by Edwardsiella anguillarum post the immunization of OmpA protecting European eel (Anguilla anguilla) from being infected. Fish and Shellfish Immunology, 118, 51–65. https://doi.org/10.1016/j.fsi.2021.08.027

Holden, C. V., Kyser, T. K., Longstaffe, F. J., Wang, Y., & Casselman, J. M. (2022). Oxygen isotope fractionation of otoliths formed across the maximum thermal range for somatic growth of the American eel Anguilla rostrata. Chemical Geology, 591. https://doi.org/10.1016/j.chemgeo.2021.120706

Inayah, Z. N., Musa, M., & Arfiati, D. (2023). Growth of Vannamei Shrimp (Litopenaeus vannamei) in Intensive Cultivation Systems. Jurnal Penelitian Pendidikan IPA, 9(10), 8821–8829. https://doi.org/10.29303/jppipa.v9i10.4278

Jamaluddin, Yuliet, Musnina, W. O. S., Yuyun, Y., Shaldan, S., Malasugi, S., Arta, P. A., Putri, G. N., Maryani, A., Haq, M. F., Lakahoro, S., Firman, N. A. P., Al-Amri, S. B., Amelia, S., Hasanuddin, A., Serdiati, N., & Nilawati, J. (2024). The Effect of Adsorbents on the Quality of Refined Eel Fish (Anguilla marmorata [Q.] Gaimard) Oil as a Raw Material for Pharmaceutical Preparations. Jurnal Penelitian Pendidikan IPA, 10(12), 10759–10792. https://doi.org/10.29303/jppipa.v10i12.9806

Kim, H. (2019). Synergistic effects of dietary two probiotics with two prebiotics to develop the synbiotics in Japanese eel, Anguilla japonica.

Kurniaji, A., Putri Renitasari, D., Saridu, S. A., Anton, A., & Yunarty, Y. (2023). The Effect of Different Probiotic Sources on Vannamei Shrimp (Litopenaeus vannamei) Cultivation with Biofloc System. Journal of Aquaculture and Fish Health, 12(3), 405–420. https://doi.org/10.20473/jafh.v12i3.37996

Kusumawaty, D., Augustine, S. M. N., Aryani, A., Effendi, Y., Emran, T. Bin, & Tallei, T. E. (2023). Configuration of gut bacterial community profile and their potential functionality in the digestive tract of the wild and cultivated Indonesian shortfin elver-phase eels (Anguilla bicolor bicolor McClelland, 1844). Biotech, 13(5). https://doi.org/10.1007/s13205-023-03561-8

Kusumawaty, D., Surtikanti, H. K., Hernawati, & Tallei, T. E. (2020). Data on community structure and diversity of the intestinal bacteria in elver and fingerling stages of wild Indonesian shortfin eel (Anguilla bicolor bicolor). Data in Brief, 29. https://doi.org/10.1016/j.dib.2020.105299

Le Moigne, L., Soulier, P., Peyraud-Waitzenegger, M., & Peyraud, C. (1986). Cutaneous and gill O2 uptake in the European eel (Anguilla anguilla L.) in relation to ambient PO2, 10-400 Torr. Respiration Physiology, 66, 341–354. https://doi.org/10.1016/0034-5687(86)90085-X

Lee, J. S., Damte, D., Lee, S. J., Hossain, M. A., Belew, S., Kim, J. Y., Rhee, M. H., Kim, J. C., & Park, S. C. (2015). Evaluation and characterization of a novel probiotic Lactobacillus pentosus PL11 isolated from Japanese eel (Anguilla japonica) for its use in aquaculture. Aquaculture Nutrition, 21(4), 444–456. https://doi.org/10.1111/anu.12176

Lee, S., Katya, K., Hamidoghli, A., Hong, J., Kim, D. J., & Bai, S. C. (2018). Synergistic effects of dietary supplementation of Bacillus subtilis WB60 and mannanoligosaccharide (MOS) on growth performance, immunity and disease resistance in Japanese eel, Anguilla japonica. Fish and Shellfish Immunology, 83, 283–291. https://doi.org/10.1016/j.fsi.2018.09.031

Lestari, P., Suprapto, D., & Mahasri, G. (2020). Isolation and identification of bacteria in gastrointestinal of eel (Anguilla bicolor) that has potential as probiotic. IOP Conference Series: Earth and Environmental Science, 441(1). https://doi.org/10.1088/1755-1315/441/1/012148

Lin, Y. J., & Tzeng, W. N. (2018). Modelling the bycatch of Anguilla marmorata using a generalized depletion model with an example from the Taiwanese glass eel fisheries for Anguilla japonica. Fisheries Research, 208, 210–218. https://doi.org/10.1016/j.fishres.2018.07.022

Lin, Y. T., Hung, Y. C., Chen, L. H., Lee, K. T., & Han, Y. S. (2024). Effects of adding Bacillus subtilis natto NTU-18 in paste feed on growth, intestinal morphology, gastrointestinal microbiota diversity, immunity, and disease resistance of Anguilla japonica glass eels. Fish and Shellfish Immunology, 149. https://doi.org/10.1016/j.fsi.2024.109556

Lin, Y. T., Pan, Y. F., & Han, Y. S. (2023). Effects of adding spermidine carbon quantum dots in feed on growth, intestinal morphology, immunity and disease resistance of Anguilla japonica and Anguilla marmorata. Aquaculture Reports, 33. https://doi.org/10.1016/j.aqrep.2023.101847

Luo, M., Guan, R., Li, Z., & Jin, H. (2013). The effects of water temperature on the survival, feeding, and growth of the juveniles of Anguilla marmorata and A. bicolor pacifica. Aquaculture, 400–401, 61–64. https://doi.org/10.1016/j.aquaculture.2013.03.003

Mandele, M. M., Amin, M., & Mahasri, G. (2024). Correlation Between Phytoplankton and Bacteria in Pond Water with the Productivity of Vaname Shrimp Ponds (Penaeus vannamei) Cultivated with an Intensive System. Jurnal Penelitian Pendidikan IPA, 10(12), 10133–10141. https://doi.org/10.29303/jppipa.v10i12.9575

Mordenti, O., Di Biase, A., Casalini, A., Emmanuele, P., Melotti, P., & Roncarati, A. (2016). Growth performances and natural diet of European eel (Anguilla anguilla L.) reared in muddy and sandy ponds. Aquatic Living Resources, 29(1). https://doi.org/10.1051/alr/2016011

Park, Y., Kim, H., Won, S., Hamidoghli, A., Hasan, M. T., Kong, I. S., & Bai, S. C. (2020). Effects of two dietary probiotics (Bacillus subtilis or licheniformis) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica. Aquaculture Nutrition, 26(2), 316–327. https://doi.org/10.1111/anu.12993

Peristiwati, Sanjaya, Y., & Trinaya, C. (2019). Isolation and identification of potential culturable probiotics bacteria from intestine of Anguilla bicolor. Journal of Physics: Conference Series, 1280(2). https://doi.org/10.1088/1742-6596/1280/2/022005

Politis, S. N., Benini, E., Miest, J. J., Engrola, S., Sørensen, S. R., Syropoulou, E., Butts, I. A. E., & Tomkiewicz, J. (2023). First Assessment of Prebiotics, Probiotics, and Synbiotics Affecting Survival, Growth, and Gene Expression of European Eel (Anguilla anguilla) Larvae. Aquaculture Research, 2023. https://doi.org/10.1155/2023/1260967

Qosimah, D., Laminem, Mandasari, C., & Setyawati, D. (2023). Review of the Role of Probiotic and Herbal Supplements as Antibacterial, Antioxidant, and Immunomodulatory Against Aeromonas hydrophila). Jurnal Penelitian Pendidikan IPA, 9(6), 178–189. https://doi.org/10.29303/jppipa.v9i6.3621

Rajkumar, M., Pandey, P. K., Aravind, R., Vennila, A., Bharti, V., & Purushothaman, C. S. (2016). Effect of different biofloc system on water quality, biofloc composition and growth performance in Litopenaeus vannamei (Boone, 1931). Aquaculture Research, 47(11), 3432–3444. https://doi.org/10.1111/are.12792

Safir, M., Maasily, I. S., Serdiati, N., & Islamy, R. A. (2024). Growth of Bamboo Lobster (Panulirus versicolor; Latreille, 1804) Fed with Feed Made from Sosodek Fish Meal (Atherinomorus lacunosus). Jurnal Penelitian Pendidikan IPA, 10(SpecialIssue), 100–106. https://doi.org/10.29303/jppipa.v10ispecialissue.7884

Saputra, S. W., Ayuningrum, D., Sabdaningsih, A., & Redjeki, S. (2024). Growth, mortality, and exploitation rate of blue swimming crab (Portunus pelagicus Linnaeus, 1758) in Jepara Regency, Central Java, Indonesia. Iranian Journal of Fisheries Sciences, 23(6), 971–983. https://doi.org/10.22092/ijfs.2024.131965

Sato, N., Kawazoe, I., Suzuki, Y., & Aida, K. (2006). Effects of temperature on vitellogenesis in Japanese eel Anguilla japonica. Fisheries Science, 72. https://doi.org/10.1111/j.1444-2906.2006.01244.x

Scabra, A. R., Budiardi, T., & Djokosetiyanto, D. (2016). Production performance of indonesian eel Anguilla bicolor bicolor with the addition of calcium carbonate (CaCO3) in to the culture media. Jurnal Akuakultur Indonesia, 15(1), 1–7. https://doi.org/10.19027/jai.15.1.7

Sekartadji, P., Ekawati, A. W., & Fadjar, M. (2023). Growth and Body Composition of Spiny Lobster (Panulirus homarus) Reared with Short-Term Fasting. Jurnal Penelitian Pendidikan IPA, 9(6), 4614–4618. https://doi.org/10.29303/jppipa.v9i6.3620

Setyono, B. D. H., Baihaqi, L. W. Al, Marzuki, M., Atmawinata, L. M., Fitria, S., & Affandi, R. I. (2023). Microbubble Technology to Improve Growth of Catfish (Clarias sp.). Jurnal Penelitian Pendidikan IPA, 9(9), 7373–7382. https://doi.org/10.29303/jppipa.v9i9.3433

Soeprijanto, A., Guntur, & Fakhri, M. (2018). Application of Probiotic and Fermented Feed in the Nursery of Anguilla bicolor. Jurnal Perikanan Universitas Gadjah Mada, 20(1), 19–22. https://doi.org/10.22146/jfs.34207

Suryadi, M. A. F. F., Mursyid, M. H., Anwar, K., Ali, M., & Kisworo, D. (2023). Isolation of Cellulolytic Bacteria from Kalkun (Meleagris gallopavo) Gastro-Intestinal Tract as a Candidate Probiotics for Poultry. Jurnal Penelitian Pendidikan IPA, 9(5), 3981–3985. https://doi.org/10.29303/jppipa.v9i5.3739

Susilo, A., Widyastuti, E. S., Evanuarini, H., & Apriliyani, M. W. (2023). Comparison of the Quality of Fermented Sausages with the Use of Yogurt Starter and Lactobacillus plantarum (pH, aw, and Proximate values). Jurnal Penelitian Pendidikan IPA, 9(5), 2319–2324. https://doi.org/10.29303/jppipa.v9i5.2463

Taufik, M., Tuan Ismail, T. I., Manan, H., Ikhwanuddin, M., Abdul Salam, A. I., Abdul Rahim, A. I., Ishak, A. N., Kamaruzzan, A. S., Draman, A. S., & Kasan, N. A. (2023). Synergistic effects of Recirculating Aquaculture System (RAS) with combination of clear water, probiotic and biofloc technology: A review. In Aquaculture and Fisheries. KeAi Communications Co. https://doi.org/10.1016/j.aaf.2023.07.006

Taufiq-Spj, N., Hutabarat, J., Trianto, A., Wirasatriya, A., Indarjo, A., Suryono, S., Ario, R., & Pratikto, I. (2020). The use of different water volume to measure the growth and survival rates of Anguilla bicolor caught from Nusawungu riverines (Vol. 13). Retrieved from http://www.bioflux.com.ro/aacl

Tesch, F.-W. (1977). The Eel (P. H. Greenwood, Ed.). Springer Netherlands. https://doi.org/10.1007/978-94-009-5761-9

Tong, R., Chen, W., Pan, L., & Zhang, K. (2020). Effects of feeding level and C/N ratio on water quality, growth performance, immune and antioxidant status of Litopenaeus vannamei in zero –water exchange bioflocs-based outdoor soil culture ponds. Fish and Shellfish Immunology, 101, 126–134. https://doi.org/10.1016/j.fsi.2020.03.051

Toro, E., Hartono, D., & Utami, M. A. F. (2024). Kajian Kualitas Air Terhadap Pertumbuhan Ikan Sidat Pada Kolam Air Mengalir. Aquacoastmarine: Journal of Aquatic and Fisheries Sciences, 3(1), 50–55. https://doi.org/10.32734/jafs.v3i1.13303

Triyatmo, B., Rustadi, & Isnansetyo, A. (2020). Effects of Probiotic Dosage on Water Quality, Total Count of Aeromonas spp. and Pseudomonas spp. in Eel Anguilla bicolor Cultivation. E3S Web of Conferences, 147. https://doi.org/10.1051/e3sconf/202014701008

Van Doan, H., Lumsangkul, C., Jaturasitha, S., Meidong, R., Hoseinifar, S. H., & Dawood, M. A. O. (2021). Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host-associated probiotic (Lactobacillus paracasei l61-27b). Aquaculture Nutrition, 27(S1), 3–12. https://doi.org/10.1111/anu.13314

Vinatea, L., Carbó, R., Andree, K. B., Gisbert, E., & Estévez, A. (2023). Rearing European Eel (Anguilla anguilla) Elvers in a Biofloc System. Animals, 13(20). https://doi.org/10.3390/ani13203234

Wang, S., He, Y., Xi, F., Liang, Y., & Zhai, S. (2023). Oligomeric Proanthocyanidins Alleviate the Detrimental Effects of Dietary Histamine on Intestinal Health of Juvenile American Eels (Anguilla rostrata). Fishes, 8(8). https://doi.org/10.3390/fishes8080413

Wang, X., Yao, Y., Ge, H., Zhang, J., Zhang, J., & Yan, Q. (2024). Isolation and identification of probiotic Bacillus subtilis AJQ03 from the intestinal tract of Anguilla japonica (Japanese eel). Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1446299

Yanti, R. Z., Muchlisin, Z. A., Nur, F. M., Irham, M., Batubara, A. S., Muhammadar, A. A., & Fadli, N. (2022). Effect Of Natural Feeds on The Growth Performance, Survival Rate And Feed Utilization Of The Tropical Shortfin Eel Anguilla Bicolor Mcclelland 1844 (Pisces: Anguillidae) Larvae. Polish Journal of Natural Sciences, 37(3), 381–390. https://doi.org/10.31648/pjns.7922

Yulia-Sugeha, H., & Suharti, S. R. (2009). Discrimination and Distribution of Two Tropical Short-Finned Eels (Anguilla bicolor bicolor and Anguilla bicolor pacifica) in the Indonesian Waters. The Nagisa Westpac Congress. Retrieved from http://hdl.handle.net/2433/144634

Zhang, L. J., Chen, Q., Yang, J. X., & Ge, J. Q. (2024). Immune responses and protective efficacy of American eel (Anguilla rostrata) immunized with a formalin-inactivated vaccine against Anguillid herpesvirus. Fish and Shellfish Immunology, 144. https://doi.org/10.1016/j.fsi.2023.109262

Zheng, C. cai, Cai, X. yi, Huang, M. meng, Mkingule, I., Sun, C., Qian, S. C., Wu, Z. ju, Han, B. nan, & Fei, H. (2019). Effect of biological additives on Japanese eel (Anguilla japonica) growth performance, digestive enzymes activity and immunology. Fish and Shellfish Immunology, 84, 704–710. https://doi.org/10.1016/j.fsi.2018.10.048

Author Biographies

Gagan Garnawansah, National Research and Innovation Agency Indonesia

Dian Permana, National Research and Innovation Agency Indonesia

Dasep Hasbullah, National Research and Innovation Agency Indonesia

Zaenal Arifin Siregar, National Research and Innovation Agency Indonesia

Dzikri Wahyudi, Karawang Marine Affairs and Fisheries Polytechnic Indonesia

Boyun Handoyo, Karawang Marine Affairs and Fisheries Polytechnic Indonesia

Indrian Rizka Amalia, National Research and Innovation Agency Indonesia

Ratna Indria Sari, National Research and Innovation Agency Indonesia

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Copyright (c) 2025 Gagan Garnawansah, Dian Permana, Dasep Hasbullah, Zaenal Arifin Siregar, Dzikri Wahyudi, Boyun Handoyo, Indrian Rizka Amalia, Ratna Indria Sari

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