Acute Toxicity Test of Lancing Leaf Extract (Solanum Mauritianum Scop.) as Well as Determination of Ld50 Values and Histopathology in Male Mice (Wistar Rat)
DOI:
10.29303/jppipa.v11i6.10776Published:
2025-06-25Issue:
Vol. 11 No. 6 (2025): JuneKeywords:
Acute toxicity, Lancing leaf extract, Ld50 valuesResearch Articles
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Abstract
The purpose of this study is to find out about the acute toxicity test and analysis of its activity on lancing leaves. This research method is an experimental method including sampling and sample processing, extract making, acute toxicity tests, research on toxic symptoms in animals, organ harvesting, organ weighing, organ histopathology examination and data analysis with Statistical Program Service Solution (SPSS). The research was conducted at the Integrated Development and Research Laboratory of Andalas University from September 2024 to December 2024. The tools used in this study are Rotary evaporator (Buchi R-210 Rotavapor), separation funnel, oven (Memmert), furnace, analytical scale (SF-400), decigator, mortar and pestle, porcelain cruce, Moisture Analyzer, Hot plate, Uv-Visible lamp (Camag), dark bottle, funnel, infusion bottle (500 ml and 100 ml), glass beaker (Pyrex), drip plate, rack and test tube, measuring cup (Pyrex), measuring flask, spatels, stirring rods, droppers, ointment pots, object glass, KLT chambers, capillary pipes, syringes, sondes, animal cages, animal feeding and drinking places. The animals that will be used in this study are healthy male white rats aged 2-3 months with a body weight of 200-300 g and have never been used for experiments as many as 25 animalsAn LD50 value was obtained with a mild toxic value (>2000 mg/KgBB – 5000 mg/KgBB) against the acute toxicity test of lancing leaf extract (Solanum Mauritianum Scop.) in male rats. There was no significant difference between groups (p>0.05) in the body and BOR of male rats compared to the control group after administration of lancing leaf extract (Solanum Mauritianum Scop.).
References
Aththorick, T. A., & Berutu, L. (2018). Ethnobotanical study and phytochemical screening of medicinal plants on Karonese people from North Sumatra, Indonesia. Journal of Physics: Conference Series, 1116(5), 052008. https://doi.org/10.1088/1742-6596/1116/5/052008
Aydin, A., Aktay, G., & Yesilada, E. (2016). A guidance manual for the toxicity assessment of traditional herbal medicines. Natural Product Communications, 11(11), 1763–1773. https://doi.org/10.1177/1934578x1601101131
Ballesteros-Ramírez, R., Lasso, P., Urueña, C., Saturno, J., & Fiorentino, S. (2024). Assessment of Acute and Chronic Toxicity in Wistar Rats (Rattus norvegicus) and New Zealand Rabbits (Oryctolagus cuniculus) of an Enriched Polyphenol Extract Obtained from Caesalpinia spinosa. Journal o f Toxicology, 2024, 1–10. https://doi.org/10.1155/2024/3769933
Dewi, P. R. P., Hairrudin, & Normasari, R. (2016). Pengaruh Stres Fisik terhadap Kadar Kreatinin Serum Tikus Wistar Jantan (Rattus norvegicus) (The Effect of Physical Stress on Serum Creatinine of Male Rattus norvegicus). Jurnal Pustaka Kesehatan, 4(2), 218–221. Retrieved from Retrieved fromhttps://jpk.jurnal.unej.ac.id/index.php/JPK/article/view/3005
El Barky, A., Hussein, S. A., Alm-Eldeen, A.-E., Hafez, A., & Mohamed, T. (2017). REVIEW Diabetes management saponins and their potential role in diabetes mellitus. Diabetes Manag, 7(1), 148–158. Retrieved from https://www.openaccessjournals.com/articles/saponins-and-their-potential-role-in-diabetes-mellitus.html
Eseberri, I., Trepiana, J., Léniz, A., Gómez-García, I., Carr-Ugarte, H., González, M., & Portillo, M. P. (2022). Variability in the Beneficial Effects of Phenolic Compounds: A Review. Nutrients, 14(9), 1925. https://doi.org/10.3390/nu14091925
Hoda, M., Hemaiswarya, S., & Doble, M. (2019). Mechanisms of Action of Phenolic Phytochemicals in Diabetes Management. In Role of Phenolic Phytochemicals in Diabetes Management. https://doi.org/10.1007/978-981-13-8997-9_4
Hussain, A., Ali, A. A., Ayaz, S., & Akram, M. (2021). Hepatoprotective effects of various medicinal plants : A systematic review Hepatoprotective effects of various medicinal plants : A systematic review. Journal of Pharmacognosy and Phytochemistry, 10(March), 109–121. Retrieved from https://www.phytojournal.com/archives/2021.v10.i3.14076/hepatoprotective-effects-of-various-medicinal-plants-a-systematic-review
Jodynis-liebert, J., & Kujawska, M. (2020). Biphasic dose-response induced by phytochemicals: Experimental evidence. Journal of Clinical Medicine, 9(3), 718. https://doi.org/10.3390/jcm9030718
Kellum, J. A., Romagnani, P., Ashuntantang, G., Ronco, C., Zarbock, A., & Anders, H. J. (2021). Acute kidney injury. Nature Reviews Disease Primers, 7(1), 52. https://doi.org/10.1038/s41572-021-00284-z
Kemenkes. (2017). Farmakope Herbal Edisi II. Direktorat Jenderal Kefarmasian dan Alat Kesehatan.
Morris-Schaffer, K., & McCoy, M. J. (2021). A Review of the LD50and Its Current Role in Hazard Communication. ACS Chemical Health and Safety, 28(1), 25–33. https://doi.org/10.1021/acs.chas.0c00096
Mustapa, M. A. (2018). Uji Toksisitas Akut Yang Diukur Dengan Penentuan Ld50 Ekstrak Etanol Bunga Cengkeh (Syzygium Aromaticum L.) Terhadap Mencit (Mus Musculus) Menggunakan Metode Thompson-Weil. Frontiers: Jurnal Sains Dan Teknologi, 1(April), 105–117. https://doi.org/10.36412/frontiers/001035e1/april201801.10
Nofita, N., Ulfa, A. M., & Muslim, D. M. (2020). Analisis sgot dan sgpt pada tikus jantan yang di induksi parasetamol untuk menetapkan aktivitas ekstrak buah delima (punica granatum l.) Sebagai hepatoprotektif. Jurnal Farmasi Malahayati, 3(1), 12–22. https://doi.org/10.33024/jfm.v3i1.3018
Oktafiano, H., Kadri, H., & Pertiwi, D. (2016). Perbedaan Kadar Glukosa Darah Antara Tikus Putih (Rattus Novergicus) yang Mendapat Asupan Susu Sapi dan Susu Kambing Segar. Jurnal Kesehatan Andalas, 5(3), 671–674. https://doi.org/10.25077/jka.v5i3.597
Perdanawati, A. L., Ratnaningtyas, N. I., & Hernayanti, H. (2022). Potensi Ekstrak Etil Asetat Coprinus Comatus Terhadap Kadar Ureum Dan Kreatinin Pada Tikus Putih Model Diabetes. BioEksakta : Jurnal Ilmiah Biologi Unsoed, 3(3), 132. https://doi.org/10.20884/1.bioe.2021.3.3.4239
Putranto, A. M. H. (2014). Examination of the Sungkai’s Young Leaf Extract (Peronema Canescens) As An Antipiretic, Immunity, Antiplasmodium And Teratogenity In Mice (Mus.muculus). International Journal of Science and Engineering, 7(1), 30–34. https://doi.org/10.12777/ijse.7.1.30-34
Sasmito, W. A., Wijayanti, A. D., Fitriana, I., & Sari, P. W. (2017). Pengujian Toksisitas Akut Obat Herbal Pada Mencit Berdasarkan Organization for Economic Co-operation and Development (OECD). Jurnal Sain Veteriner, 33(2). https://doi.org/10.22146/jsv.17924
Smakosz, A., Matkowski, A., & Nawrot-Hadzik, I. (2024). Phytochemistry and Biological Activities of Agrostemma Genus—A Review. Plants, 13(12), 1673. https://doi.org/10.3390/plants13121673
Syahroni, A., Februyani, N., & Zuhriyah, A. (2023). Uji Toksisitas Akut Teh Herbal Antipiretik Kombinasi Tanaman Sereh Dan Kemangi Pada Mencit. Indonesian Journal of Health Science, 3(2a), 409–415. https://doi.org/10.54957/ijhs.v3i2a.539
Author Biographies
Afifah Ikhwan, Universitas Prima Indonesia
Erny Tandanu, Universitas Prima Indonesia
Master of Biomedicine, Faculty of Medicine, Universitas Prima Indonesia
Ali Napiah Nasution, Universitas Prima Indonesia
Refi Ikhtiari, Universitas Prima Indonesia
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