Assessment of Vocational High School Tissue Culture Laboratory Readiness for Learning and Entrepreneurship: A Descriptive Study of Infrastructure and Management Standards
DOI:
10.29303/jppipa.v12i5.11395Published:
2026-05-31Downloads
Abstract
This research evaluates the readiness of the tissue culture laboratory at SMK Negeri 1 Pandak using an observation instrument of laboratory preparedness. This study focused on the laboratory infrasturucture and management system as the primary object of observation in seven aspects. The instrument's validation was conducted by two Expert of Tissue Culture experts to yield Aiken's V validity coefficients for the seven aspects of laboratory readiness observation instrument ranging from 0.585 to 0.661, indicating that the instrument is valid. Observations of the tissue culture laboratory's readiness also revealed an average total readiness percentage of 76.72%, with the infrastructure (84.61%), facilities (85.84%), and administration (85.71%) aspects categorized as "Very Ready". The occupational health and safety (70%), learning process support (80%), and entrepreneurship development (68.42%) aspects are in the "Ready" category. The laboratory dynamics aspect (62.5%) showed a "Less Ready" category, thus requiring special attention. This research confirms the validity of the instrument and provides an overview of the tissue culture laboratory's readiness, and it can be used to enhance the quality and effectiveness of tissue culture laboratories in vocational agriculture schools. In addition, this result contributes as a strategic evaluation model in the management of vocational education laboratories and provides concrete recommendations for the development of entrepreneurship-based science education through the optimization of facilities and standardized management systems
Keywords:
Availability Laboratory Plant tissue cultureReferences
Abidin, A. U., & Ramadhan, I. (2019). Penerapan Job Safety Analysis, Pengetahuan Keselamatan dan Kesehatan Kerja Terhadap Kejadian Kecelakaan Kerja di Laboratorium Perguruan Tinggi. Jurnal Berkala Kesehatan, 5(2), 76. https://doi.org/10.20527/jbk.v5i2.7827 DOI: https://doi.org/10.20527/jbk.v5i2.7827
Acquier, A., Carbone, V., & Massé, D. (2019). How to create value (s) in the sharing economy: Business models, scalability, and sustainability. Technology innovation management review, 9(2), 5-22. http://doi.org/10.22215/timreview/1215 DOI: https://doi.org/10.22215/timreview/1215
Ahmed, S. H. (2024). Enhancing Emergency Evacuation Routes Through Integration of Safety and Adaptive Characteristics in The Architectural Design of Educational Buildings. International Design Journal, 14(2), 429–438. https://doi.org/10.21608/idj.2023.253101.1104 DOI: https://doi.org/10.21608/idj.2023.253101.1104
Aiken, L. R. (1985). Three Coefficients for Analyzing the Reliability and Validity of Ratings. Educational and Psychological Measurement, 45(1), 131–142. https://doi.org/10.1177/0013164485451012 DOI: https://doi.org/10.1177/0013164485451012
Akase, S., & Kpera, T. (2024). Ensuring Accuracy, Ensuring Life; a Crucial Role of Quality Management Systems in Medical Laboratories. ScienceOpen Preprints. https://doi.org/10.14293/PR2199.001258.v1 DOI: https://doi.org/10.14293/PR2199.001258.v1
Alemneh, C., Munye, T., Mohammed, N., Shasho, G., Gizaw, E., & Siraj, Z. (2025). Improving Students' Engagement Level in Laboratory Activities a Case Study of Second‐Year Biology Department Students in the 2025 GC Academic Year, Science. Journal of Education, 13(4), 117-125. https://doi.org/10.11648/j.sjedu.20251304.11 DOI: https://doi.org/10.11648/j.sjedu.20251304.11
Alič, Š., Naglič, T., Tušek-Žnidarič, M., Peterka, M., Ravnikar, M., & Dreo, T. (2017). Putative new species of the genus Dickeya as major soft rot pathogens in Phalaenopsis orchid production. Plant Pathology, 66(8), 1357–1368. https://doi.org/10.1111/ppa.12677 DOI: https://doi.org/10.1111/ppa.12677
Alifatunnisa, N. A., Royani, A., Noviliani, Y., Nazhifah, S. S. Z., & Muliyah, E. (2024). Analisis Pengelolaan Limbah Praktikum Biologi di Madrasah Aliyah X, Tangerang Selatan. INSOLOGI: Jurnal Sains Dan Teknologi, 3(4), 412–419. https://doi.org/10.55123/insologi.v3i4.3902 DOI: https://doi.org/10.55123/insologi.v3i4.3902
Alpha, H. E., Ibeh, N. J., Ochekwu, E. B., & Amakoromo, T. E. (2025). A Review on Optimizing Laboratory Layouts for Enhanced Workflow and Safety. Researchers Journal of Science and Technology, 5(4), 73–91. Retrieved from https://rejost.com.ng
Álvarez-Chávez, C. R., Marín, L. S., Perez-Gamez, K., Portell, M., Velazquez, L., & Munoz-Osuna, F. (2019). Assessing College Students’ Risk Perceptions of Hazards in Chemistry Laboratories. Journal of Chemical Education, 96(10), 2120–2131. https://doi.org/10.1021/acs.jchemed.8b00891 DOI: https://doi.org/10.1021/acs.jchemed.8b00891
Amalatus, R. B., Alifha, R. N., Ningsih, I. S., Hartono, A., & Ikbal, M. (2021). Analisis Studi Kelayakan Laboratorium Ilmu Pengetahuan Alam di SMPN 2 Tempurejo. VEKTOR: Jurnal Pendidikan IPA, 2(1), 49–55. https://doi.org/10.35719/vektor.v2i1.25 DOI: https://doi.org/10.35719/vektor.v2i1.25
Amaliah., Muarif, I., Sariwardani, A., & Rusdi, M. (2026). The influence of teaching competence, learning motivation, and learning environment on students’ academic achievement. Journal Management & Economics Review (JUMPER), 3(10. 1), 1-14. https://doi.org/10.59971/jumper.v3i10.%201.980
Andriani, D., & Heriansyah, P. (2021). Identifikasi Jamur Kontaminan pada Berbagai Eksplan Kultur Jaringan Anggrek Alam (Bromheadia finlaysoniana (Lind.) Miq. Agro Bali : Agricultural Journal, 4(2), 192–199. https://doi.org/10.37637/ab.v4i2.723 DOI: https://doi.org/10.37637/ab.v4i2.723
Anggereni, S., Suhardiman, S., & Amaliah, R. (2021). Analisis Ketersediaan Peralatan, Bahan Ajar, Administrasi Laboratorium, Keterlaksanaan Kegiatan Praktikum di Laboratorium Fisika. Jurnal Ilmiah Pendidikan Fisika, 5(3), 414. https://doi.org/10.20527/jipf.v5i3.3925 DOI: https://doi.org/10.20527/jipf.v5i3.3925
Anis, M. (2021). Impact of plant tissue culture on advances in plant biology. The Journal of the Indian Botanical Society, 101(04), 254–268. https://doi.org/10.5958/2455-7218.2021.00032.2
Araoye, T., & Egoigwe, S. V. (2024). Integrating advanced control strategies for autonomous agricultural robotics into nigerian educational reforms: challenges and opportunities. International Journal of Studies in Education, 20(2), 138–147. Retrieved from https://ijose.unn.edu.ng/wp-content/uploads/sites/224/2024/07/Paper-20.2.14.pdf
Azahra, P. S., & Ernayunita. (2023). Upaya meminimalkan abnormalitas pada klon kelapa sawit. WARTA Pusat Penelitian Kelapa Sawit, 28(1), 55–62. https://doi.org/https://doi.org/10.22302/iopri.war.warta.v28i1.102 DOI: https://doi.org/10.22302/iopri.war.warta.v28i1.102
Bai, M., Liu, Y., Qi, M., Roy, N., Shu, C.-M., Khan, F., & Zhao, D. (2022). Current status, challenges, and future directions of university laboratory safety in China. Journal of Loss Prevention in the Process Industries, 74, 104671. https://doi.org/https://doi.org/10.1016/j.jlp.2021.104671 DOI: https://doi.org/10.1016/j.jlp.2021.104671
Basri, A. H., Wismoyo, E. A., & Firmansyah, R. (2020). Perancangan ulang interior rumah sakit di Cipacing, Sumedang Jawa Barat. Proceedings of Art & Design. Retrieved from https://ijose.unn.edu.ng/wp-content/uploads/sites/224/2024/07/Paper-20.2.14.pdf
Bednarek, P. T., & Orłowska, R. (2020). Plant tissue culture environment as a switch-key of (epi)genetic changes. Plant Cell, Tissue and Organ Culture (pp. 245–257). Springer. https://doi.org/10.1007/s11240-019-01724-1 DOI: https://doi.org/10.1007/s11240-019-01724-1
Beyl, C. A. (2018). Getting started with tissue culture—media preparation, sterile technique, and laboratory equipment. In Plant tissue culture concepts and laboratory exercises (pp. 21–38). Routledge.
Bhojwani, S. S., & Dantu, P. K. (2013). Plant tissue culture: an introductory text. Springer. DOI: https://doi.org/10.1007/978-81-322-1026-9
Bidabadi, S. S., & Jain, S. M. (2020). Cellular, molecular, and physiological aspects of in vitro plant regeneration. MDPI, 9(6), 702. https://doi.org/10.3390/plants9060702 DOI: https://doi.org/10.3390/plants9060702
Camilleri, M. A., Troise, C., Strazzullo, S., & Bresciani, S. (2023). Creating shared value through open innovation approaches: Opportunities and challenges for corporate sustainability. Business Strategy and the Environment, 32(7), 4485–4502. https://doi.org/10.1002/bse.3377 DOI: https://doi.org/10.1002/bse.3377
Cholifah, A., Widjanarko, D., & Anis, S. (2025). Development of a Virtual Tissue Culture Laboratory to Improve the Cognitive Abilities of Vocational School Students Studying Plant Agribusiness. Journal of Vocational and Career Education, 10(2), 145–150. https://doi.org/https://doi.org/10.15294/jvce.v10i2.32412 DOI: https://doi.org/10.15294/jvce.v10i2.32412
De Filippis, L. F. (2014). Crop improvement through tissue culture. In Improvement of Crops in the Era of Climatic Changes, Volume 1 (pp. 289–346). Springer New York. https://doi.org/10.1007/978-1-4614-8830-9_12 DOI: https://doi.org/10.1007/978-1-4614-8830-9_12
Desai, S., Hatfield, L. A., Hicks, A. L., Chernew, M. E., & Mehrotra, A. (2016). Association between availability of a price transparency tool and outpatient spending. Jama, 315(17), 1874–1881. https://doi.org/https://doi.org/10.1001/jama.2016.4288 DOI: https://doi.org/10.1001/jama.2016.4288
Dhanakoses, K., Povatong, P., Wongphyat, W., & Wiriyakraikul, C. (2023). Building adaptations and laboratory safety concerns: A case study of high-rise academic laboratories in a Thai University. Nakhara: Journal of Environmental Design and Planning, 22(2), 312–312. https://doi.org/10.54028/NJ202322312 DOI: https://doi.org/10.54028/NJ202322312
El-Sherif, N. A. (2019). Impact of plant tissue culture on agricultural sustainability. In Handbook of Environmental Chemistry (pp. 93–107). Springer Verlag. https://doi.org/10.1007/698_2017_160 DOI: https://doi.org/10.1007/698_2017_160
Fayaz, H., Kumar, A., Kousar, F., Sharma, S., & Kumar, S. (2020). Application of total quality management to ensure food quality in food industry. Journal of Animal Research, 10(3), 329–338. https://doi.org/10.30954/2277-940x.03.2020.1 DOI: https://doi.org/10.30954/2277-940X.03.2020.1
Gailea, S. (2023). Evaluasi dan optimalisasi penggunaan laboratorium IPA di MTSN 1 Kepulauan Sula. Juanga : Jurnal Agama Dan Ilmu Pengetahuan, 9(2), 133–144. Retrieved from http://e-jurnal.staibabussalamsula.ac.id/index.php/JUANGA/article/view/151
Gisev, N., Bell, J. S., & Chen, T. F. (2013). Interrater agreement and interrater reliability: Key concepts, approaches, and applications. Research in Social and Administrative Pharmacy, 9(3), 330–338. https://doi.org/10.1016/j.sapharm.2012.04.004 DOI: https://doi.org/10.1016/j.sapharm.2012.04.004
Gusmanto, R. (2023). Manajemen pengelolaan alat dan bahan di laboratorium Fakultas Tarbiyah dan Ilmu Keguruan (FTIK) IAIN Kerinci. Leader: Jurnal Manajemen Pendidikan Islam, 1(2), 112–121. https://doi.org/10.32939/ljmpi.v1i2.3084 DOI: https://doi.org/10.32939/ljmpi.v1i2.3084
Halek, E., & Naimnule, L. (2022). Sosialisasi pemanfaatan alat dan bahan laboratorium sebagai sarana penunjang pembelajaran biologi bagi siswa SMA Negeri 2 Kefamenanu. Jurnal Pengabdian Sains Dan Humaniora, 1(2), 72–77. https://doi.org/https://doi.org/10.32938/jpsh.1.2.2022.72-77 DOI: https://doi.org/10.32938/jpsh.1.2.2022.72-77
Harahap, F., Insani, H., Diningrat, D. S., Nasution, N. E. A., Poerwanto, R., & Hasibuan, R. F. M. (2020). Needs assessment of teaching book development based on plants multiplication research in plant tissue culture course. In 2nd Educational Sciences International Conference (ESIC 2019) (pp. 17–21). Atlantis Press. DOI: https://doi.org/10.2991/assehr.k.200417.005
Hasrawati, Masriany, Hafsan, & Nur, F. (2022). Pemberian ekstrak lada hitam (Piper nigrum L.) untuk menekan laju pertumbuhan kontaminan pada kultur in vitro tanaman kentang (Solanum tuberosum). Filogeni: Jurnal Mahasiswa Biologi, 2(1), 15–20. https://doi.org/10.24252/filogeni.v2i1.28630 DOI: https://doi.org/10.24252/filogeni.v2i1.28630
Ikram, M., & Kenayathulla, H. B. (2023, March). Education quality and student satisfaction nexus using instructional material, support, classroom facilities, equipment and growth: Higher education perspective of Pakistan. In Frontiers in Education (Vol. 8, p. 1140971). Frontiers Media SA. https://doi.org/10.3389/feduc.2023.1140971 DOI: https://doi.org/10.3389/feduc.2023.1140971
Indriastuti, I., Herlina, L., & Widiyaningrum, P. (2013). Kesiapan laboratorium biologi dalam menunjang kegiatan praktikum SMA Negeri di Kabupaten Brebes. Journal of Biology, 2(2), 50–229. https://doi.org/https://doi.org/10.15294/jbe.v2i2.2834
Isma, T. W., Tasrif, E., Huda, Y., & Syah, N. (2021). Analisis Konten Modul Pelajaran Mikrokontroller Terhadap Keterampilan Berpikir Kritis dan Kreativitas Siswa Sekolah Menengah Kejuruan. Edukatif : Jurnal Ilmu Pendidikan, 4(1), 582–589. https://doi.org/10.31004/edukatif.v4i1.1891 DOI: https://doi.org/10.31004/edukatif.v4i1.1891
Ismail, R., & Prihatini, E. (2023). Tata Kelola Bahan Kimia di Laboratorium dengan Sistem Dokumen dan Perangkat Lunak Chemical Inventory Management System (CIMS) Berbasis Excel. Indonesian Journal of Laboratory, 3, 135–144. https://doi.org/https://doi.org/10.22146/ijl.v0i3.82022 DOI: https://doi.org/10.22146/ijl.v0i3.82022
Ismarau Tajuddin, N. I., Abas, U.-H., Aziz, K. A., Haizan Nor, R. N., Izni, N. A., Sudin, M. N., … & Noor, N. M. (2025). Content Validity Assessment Using Aiken’s V: Knowledge Integration Model for Blockchain in Higher Learning Institutions. (IJACSA) International Journal of Advanced Computer Science & Applications, 16(6). https://doi.org/10.14569/ijacsa.2025.0160659 DOI: https://doi.org/10.14569/IJACSA.2025.0160659
Kustiani, E. (2020). Kultur Jaringan: Teori & Praktek. UNIK press.
Laila, N. N. (2021). Manajemen Laboratorium dalam Aspek Keselamatan dan Kesehatan Kerja pada Laboratorium Pendidikan. Penerbit NEM.
Lewis, P. (2023). Innovation, technician skills, and vocational education and training: connecting innovation systems and vocational education and training. Journal of Vocational Education and Training, 77(2), 1–28. https://doi.org/10.1080/13636820.2023.2215749 DOI: https://doi.org/10.1080/13636820.2023.2215749
Li, M., Gao, Q., & Yu, T. (2023). Kappa statistic considerations in evaluating inter-rater reliability between two raters: which, when and context matters. BMC Cancer, 23(1), 799. https://doi.org/10.1186/s12885-023-11325-z DOI: https://doi.org/10.1186/s12885-023-11325-z
Lowe, D., Newcombe, P., & Stumpers, B. (2013). Evaluation of the use of remote laboratories for secondary school science education. Research in Science Education, 43(3), 1197–1219. https://doi.org/10.1007/s11165-012-9304-3 DOI: https://doi.org/10.1007/s11165-012-9304-3
Lutfhianti, S. N., Nurlaeli, A., & Ma’shum, S. (2024). Analisis efektivitas manajemen laboratorium sains dalam memperkuat keterampilan siswa di MAN 21 Jakarta. Indonesian Research Journal on Education, 4(2), 144–150. https://doi.org/https://doi.org/10.31004/irje.v4i2.505 DOI: https://doi.org/10.31004/irje.v4i2.505
Lv, M., Zhang, H., Georgescu, P., Li, T., & Zhang, B. (2022). Improving education for innovation and entrepreneurship in Chinese technical universities: A quest for building a sustainable framework. Sustainability, 14(2), 595. https://doi.org/10.3390/su14020595 DOI: https://doi.org/10.3390/su14020595
Makmur, & Mulyaningrum, S. R. H. (2018). Evaluasi performa bibit rumput laut Gracilaria verrucosa hasil kultur jaringan di Kabupaten Luwu, Sulawesi Selatan. Media Akuakultur, 13(1), 1–9. https://doi.org/http://dx.doi.org/10.15578/ma.13.1.2018.1-9 DOI: https://doi.org/10.15578/ma.13.1.2018.1-9
Marcolan, J. A., Marques, J. L. B., & Marino-Neto, J. (2022). Assessing the quality of behavioral data obtained by human observers using Cohen’s Kappa and accessory metrics: development of the algorithms and an open-source library. In Latin American Conference on Biomedical Engineering (pp. 413–423). Cham: Springer Nature Switzerland. https://doi.org/https://doi.org/10.1007/978-3-031-49410-9_43 DOI: https://doi.org/10.1007/978-3-031-49410-9_43
Muhammad, A. (1992). Penelitian Pendidikan dan Strategi. Bandung: Rineka Cipta.
Mukherjee, S., Malik, P., & Mukherjee, T. K. (2023). Establishment of a cell culture laboratory. In Practical Approach to Mammalian Cell and Organ Culture (pp. 43–82). Singapore: Springer Nature Singapore. DOI: https://doi.org/10.1007/978-981-19-1731-8_2-1
Mulyaningrum, S. R. H., Daud, R., & Badraeni. (2014). Propagasi vegetatif rumput laut Gracilaria sp. melalui kultur jaringan. Jurnal Riset Akuakultur, 9(2), 203–214. https://doi.org/http://dx.doi.org/10.15578/jra.9.2.2014.203-214 DOI: https://doi.org/10.15578/jra.9.2.2014.203-214
Nadillah, S., Nuraeni, S., & Oktorida, R. (2022). Pentingnya memahami bahaya bahan kimia serta hubungannya dengan kesehatan dan keselamatan kerja di laboratorium. Jurnal Analis Laboratorium Medik, 7(1), 15–22. https://doi.org/10.51544/jalm.v7i1.2430 DOI: https://doi.org/10.51544/jalm.v7i1.2430
Nasir, M., & Mayasri, A. (2023). Pelatihan standardisasi pengelolaan laboratorium bagi pengelola laboratorium IPA di Kota Banda Aceh. Abdimasku: Jurnal Pengabdian Masyarakat, 6(3), 1058–1063. https://doi.org/https://doi.org/10.62411/ja.v6i3.1336 DOI: https://doi.org/10.62411/ja.v6i3.1336
Navlekar, A. S., Theobald, E. J., Griffith, K., & Limeri, L. B. (2025). Strategically creating maximally heterogeneous lab groups did not improve group performance in an introductory biology lab class. Plos one, 20(5), e0323799. https://doi.org/10.1371/journal.pone.0323799 DOI: https://doi.org/10.1371/journal.pone.0323799
Nazri, E., Setiowati, R. D., Ernayunita, Rahmadi, H. Y., & Yenni, Y. (2022). The role of ortet sources, fronds and incubation to callus production of oil palm (Elaeis guineensis Jacq.). Jurnal Penelitian Kelapa Sawit, 30(3), 153–160. https://doi.org/https://doi.org/10.22302/iopri.jur.jpks.v30i3.186 DOI: https://doi.org/10.22302/iopri.jur.jpks.v30i3.186
Nggolaon, D., Silahooy, S., & Jaya, G. W. (2024). Edukasi Peralatan Pengukuran dengan Metode Praktikum di Sma Negeri 27 Maluku Tengah. PaKMas: Jurnal Pengabdian Kepada Masyarakat, 4(2), 331–337. https://doi.org/10.54259/pakmas.v4i2.3048 DOI: https://doi.org/10.54259/pakmas.v4i2.3048
Novitasari, A., Nurjanah, S., & Solviana, M. D. (2024). Analisis Manajemen Laboratorium Biologi di Sekolah Menengah Atas di Kota Bandar Lampung. LENSA(Lentera Sains): Jurnal Pendidikan IPA, 14(2), 69–74. https://doi.org/10.24929/lensa.v14i2.469
Nuevo, E. B. T. (2024). Adequacy, utilization of laboratory equipment and performance of junior high school science teachers. International Journal for Multidisciplinary Research, 6(3), 1-32. https://doi.org/10.36948/ijfmr.2024.v06i03.21166 DOI: https://doi.org/10.36948/ijfmr.2024.v06i03.21166
Nuriyanti, L., Prayitno, S., Tyaningsih, R. Y., & Sarjana, K. (2022). Pengembangan Media Pembelajaran Matematika Berbasis Powtoon Pada Materi Statistika. Jurnal Ilmiah Profesi Pendidikan, 7(3), 1462–1471. https://doi.org/10.29303/jipp.v7i3b.808 DOI: https://doi.org/10.29303/jipp.v7i3b.808
Nurjanah, S., Istiyono, E., Widihastuti, W., Iqbal, M., & Kamal, S. (2023). The application of Aiken’s V method for evaluating the content validity of instruments that measure the implementation of formative assessments. Journal of Research and Educational Research Evaluation, 12(2), 125–133. https://doi.org/10.15294/jere.v12i2.76451
Oratmangun, K. M., Pandiangana, D., & Kandou, F. E. (2017). Deskripsi Jenis-Jenis Kontaminan Dari Kultur Kalus Catharanthus Roseus (L.) G. Donnaman. Jurnal MIPA Unsrat Online, 6(1), 47–52. https://doi.org/10.35799/jm.6.1.2017.16154 DOI: https://doi.org/10.35799/jm.6.1.2017.16154
Osaiyuwu, R. (2025). The Impact Of Inadequate Laboratory Facility On Students' Academic Performance In Integrated Science In Federal College Of Education (Technical), Omoku, Rivers State. International Journal of Innovative Social & Science Education Research, 13(4), 297-310. https://doi.org/10.5281/zenodo.18033356
Pamies, D., Leist, M., Coecke, S., Bowe, G., Allen, D. G., Gstraunthaler, G., … Stacey, G. (2022). Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0). Altex, 39(1), 30–70. https://doi.org/10.14573/altex.2111011 DOI: https://doi.org/10.14573/altex.2111011
Pavlova, M. (2019). Emerging environmental industries: impact on required skills and TVET systems. International Journal of Training Research, 17(sup1), 144–158. https://doi.org/10.1080/14480220.2019.1639276 DOI: https://doi.org/10.1080/14480220.2019.1639276
Perry, J., & Baum, J. (2018). Assessing the laboratory environment. Accessibility in the Laboratory, 1272, 25–60. https://doi.org/10.1021/bk-2018-1272.ch003 DOI: https://doi.org/10.1021/bk-2018-1272.ch003
Piccinno, F., Hischier, R., Seeger, S., & Som, C. (2016). From laboratory to industrial scale: a scale-up framework for chemical processes in life cycle assessment studies. Journal of Cleaner Production, 135, 1085–1097. https://doi.org/10.1016/j.jclepro.2016.06.164 DOI: https://doi.org/10.1016/j.jclepro.2016.06.164
Pong-Masak, P. R., Priono, B., & Insan, I. (2011). Seleksi klon bibit rumput laut, Gracilaria verrucosa. Media Akuakultur, 6(1), 1–12. http://dx.doi.org/10.15578/ma.6.1.2011.1-12 DOI: https://doi.org/10.15578/ma.6.1.2011.1-12
Prasetyo, I. (2021). Pelatihan penggunaan alat uji emisi gas buang kendaraan bagi tenaga laboran SMK se-kota dan Kabupaten Pekalongan. Abdi Masya, 1(2), 47–51. https://doi.org/10.52561/abma.v1i2.126 DOI: https://doi.org/10.52561/abma.v1i2.126
Pratiwi, A. Y., & Mulyono, R. (2023). Implementasi pola POAC dalam manajemen laboratorium di SMA Kesatuan Bangsa. Didaktik: Jurnal Ilmiah PGSD STKIP Subang, 9(1), 707–716. https://doi.org/https://doi.org/10.36989/didaktik.v9i1.723 DOI: https://doi.org/10.36989/didaktik.v9i1.723
Raharjo. (2017). Pengelolaan alat bahan dan laboratorium kimia. Jurnal Kimia Sains Dan Aplikasi, 20, 99–104. https://doi.org/https://doi.org/10.14710/jksa.20.2.99-104 DOI: https://doi.org/10.14710/jksa.20.2.99-104
Rahayu, E. S., Rahayuningsih, M., Noekent, V., & Al Muhammady, A. R. (2019). Developing entrepreneurial competencies of Biology Study Program students through a business unit on plant tissue culture training. Journal of Physics: Conference Series, 1321(3). Institute of Physics Publishing. https://doi.org/10.1088/1742-6596/1321/3/032041 DOI: https://doi.org/10.1088/1742-6596/1321/3/032041
Rahmi, H. (2023). Pelatihan Teknik Kultur Jaringan Tanaman Kawista di Kelurahan Adiarsa Barat, Kecamatan Karawang Barat. Jurnal Budiman: Pembangunan Dan Pengabdian Masyarakat Nusantara, 1(2), 28–33. https://doi.org/10.35706/budiman.v1i2.10225 DOI: https://doi.org/10.35706/budiman.v1i2.10225
Rau, G., & Shih, Y.-S. (2021). Evaluation of Cohen’s kappa and other measures of inter-rater agreement for genre analysis and other nominal data. Journal of English for Academic Purposes, 53, 101026. https://doi.org/10.1016/j.jeap.2021.101026 DOI: https://doi.org/10.1016/j.jeap.2021.101026
Rizki, M., Suryawati, E., Zulfarina, Z., & Rahmi, F. O. (2021). Analisis self efficacy sebagai dasar pengembangan sumber belajar mandiri untuk praktikum jarak jauh di LPTK. Jurnal Pendidikan Biologi, 12(2), 71–80. https://doi.org/10.17977/um052v12i2p71-80 DOI: https://doi.org/10.17977/um052v12i2p71-80
Roy, S., Mandal, B., & Das, B. K. (2025). Quality assurance in laboratory practices and equipment maintenance: Ensuring precision, reliability, and compliance. In Laboratory Techniques for Fish Disease Diagnosis (pp. 79–103). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-96-4620-3_5 DOI: https://doi.org/10.1007/978-981-96-4620-3_5
Samanhudi, Widijanto, H., & Yunus, A. (2020). Sosialisasi dan penyuluhan budidaya pisang dengan bibit hasil kultur jaringan di Desa Lempong, Kecamatan Jenawi, Kabupaten Karanganyar. PRIMA: Journal of Community Empowering and Services, 4(2), 59–63. https://doi.org/10.20961/prima.v4i2.44369 DOI: https://doi.org/10.20961/prima.v4i2.44369
Sari, E., Mei, G., Dakhi, M., Azkia, F., Bulkiah, S., Purbaya, R., & Sulaeman, N. F. (2024). Safety First: Assessing and Comparing Laboratories in Junior High Schools in Samarinda City. ScienceEdu, 7(1), 16–22.
Sari, I. P., Sulistiani, E., Syaifulla, A. P., Putri, R. A., Baruku, D., Anwar, A., & Sulaeman, N. F. (2023). Analisis Ketersediaan Fasilitas dan Alat Praktikum Laboratorium Fisika Untuk SMA di Kota Samarinda. Jurnal Literasi Pendidikan Fisika (JLPF), 4(2), 88–95. https://doi.org/10.30872/jlpf.v4i2.1646 DOI: https://doi.org/10.30872/jlpf.v4i2.1646
Sarifudin, & Suhri, A. (2024). Revitalisasi Sekolah Berbasis Teaching Factory (TEFA) Sebagai Rujukan Sekolah Menengah Kejuruan Swasta di Pamekasan (Studi Kasus di SMKS Mambaul Ulum Bata-Bata Panaan Palengaan Pamekasan). Studia Religia: Jurnal Pemikiran Dan Pendidikan Islam, 8(2), 317–334. https://doi.org/10.30651/sr.v8i2.24559 DOI: https://doi.org/10.30651/sr.v8i2.24559
Sa’Roni, A. (2020). Penerapan Pertolongan Pertama Pada Kecelakaan di Tempat Kerja. HIGEIA (Journal of Public Health Research and Development), 4(1), 247–261. https://doi.org/10.15294/higeia.v4iSpecial%201/39412
Semiarti, E. (2022). Optimalisasi pemanfaatan teknik kultur jaringan tumbuhan dalam mendukung SDGs 2030 melalui sains dan entrepreneurship. In Seminar Nasional Sains dan Entrepreneurship. Retrieved from https://conference.upgris.ac.id/index.php/snse/article/download/3400/1849
Seprianto, Saraswati, H., Wahyuni, F. D., Novianti, T., Nora, A., Naroeni, A., & Handayani, P. (2022). Workshop Isolasi DNA dan Pengenalan Alat Laboratorium Bioteknologi Bagi Guru Biologi SMA/MA Se Jakarta. Jurnal Pengabdian Kepada Masyarakat, 2(3), 4503–4514. https://doi.org/10.53625/jabdi.v2i3.2780 DOI: https://doi.org/10.53625/jabdi.v2i3.2780
Sholehah, H., Siswandi, E., Azwaruddin, A., & Nurhidayatullah, N. (2022). Pembuatan sistem jaringan instalasi pengolahan air limbah laboratorium lingkungan. Community Jurnal Pengabdian Kepada Masyarakat, 1(2), 113–118. https://doi.org/10.51878/community.v1i2.855 DOI: https://doi.org/10.51878/community.v1i2.855
Sipangkar, V. V., Wijaya, I. N., & Sritamin, M. (2020). Kultur Jaringan Jeruk Keprok Tejakula (Citrus Reticulata Var. Tejakula) Menggunakan Tunas Muda dan Biji Serta Deteksi CVPD dengan Teknik Polymerase Chain Reaction (PCR). Agrotrop: Journal on Agriculture Science, 10(1), 49–58. https://doi.org/10.24843/ajoas.2020.v10.i01.p06 DOI: https://doi.org/10.24843/AJoAS.2020.v10.i01.p06
Sucahyo, A. I., Manalu, K., & Nasution, R. A. (2023). Isolasi dan Identifikasi Mikroba Penyebab Kontaminasi dari Udara di Laboratorium Kultur Jaringan Tumbuhan UIN-SU Medan. Jurnal Biologi, 1(1), 1–12. https://doi.org/10.47134/biology.v1i1.1931 DOI: https://doi.org/10.47134/biology.v1i1.1931
Sumual, S. D. M., Tuerah, P. R., Pontoh, L. F., Taroreh, O., Repi, M. F., & Mesra, R. (2023). Implikasi terbatasnya infrastruktur dalam upaya mencapai tujuan pendidikan. JUPE: Jurnal Pendidikan Mandala, 8(2), 418–424. Retrieved from http://ejournal.mandalanursa.org/index.php/JUPE/index DOI: https://doi.org/10.58258/jupe.v8i2.5261
Suslistya, V., & Mahadewi, G. (2023). Manajemen laboratorium sebagai langkah peningkatan mutu pelaksanaan praktikum ilmu pengetahuan alam. SEARCH: Science Education Research Journal, 1(2), 1–13. https://doi.org/https://doi.org/10.47945/search.v1i2.1247 DOI: https://doi.org/10.47945/search.v1i2.1247
Syakbania, D. N., & Wahyuningsih, A. S. (2017). Program keselamatan dan kesehatan kerja di laboratorium kimia. HIGEIA (Journal of Public Health Research and Development), 1, 49–57. Retrieved from http://journal.unnes.ac.id/sju/index.php/higeia
Tang, Q., Yan, F., Yuan, L., Tang, Y., Chen, H., Sun, Y., ... & Song, G. (2024). Enhancing laboratory biosafety management: a comprehensive strategy from theory to practice. Frontiers in public health, 12, 1439051. https://doi.org/10.3389/fpubh.2024.1439051 DOI: https://doi.org/10.3389/fpubh.2024.1439051
Tegen, H., & Mohammed, W. (2016). The role of plant tissue culture to supply disease free planting materials of major horticultural crops in Ethiopia. Journal of Biology, Agriculture and Healthcare, 6(1), 122–129. Retrieved from www.iiste.org
Tiwari, A., Tikoo, S. K., Angadi, S. P., Kadaru, S. B., Ajanahalli, S. R., & Vasudeva Rao, M. J. (2023). Plant breeding: Its evolution and recent trends. In Market-Driven Plant Breeding for Practicing Breeders (pp. 1–32). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-19-5434-4_1 DOI: https://doi.org/10.1007/978-981-19-5434-4_1
Torres-Malca, J. R., Vera-Ponce, V. J., Zuzunaga-Montoya, F. E., Talavera, J. E., & De La Cruz-Vargas, J. A. (2022). Content validity by expert judgment of an instrument to measure knowledge, attitudes and practices about salt consumption in the peruvian population. Revista de La Facultad de Medicina Humana, 22(2), 367–373. https://doi.org/10.25176/RFMH.v22i2.4768 DOI: https://doi.org/10.25176/RFMH.v22i2.4768
Walsh, F. (2024). Strategic Partnerships in Biotechnology: Analyzing Trends in Collaborative R& D and Market Expansion. Journal of Commercial Biotechnology, 29(6), 231-240. https://10.5912/jcb2474
Wanyama, J. M. (2015). An evaluation of impact and challenges of tissue culture banana technology in Kenya. Retrieved from http://ir-library.egerton.ac.ke/handle/123456789/1287
Weiskirchen, S., Schröder, S. K., Buhl, E. M., & Weiskirchen, R. (2023). A beginner’s guide to cell culture: Practical advice for preventing needless problems. Cells, 12(5), 682. https://doi.org/10.3390/cells12050682 DOI: https://doi.org/10.3390/cells12050682
Wulandari, S., Nisa, Y. S., Taryono, Indarti, S., & Sayekti, R. R. S. (2022). Sterilisasi peralatan dan media kultur jaringan. Agrinova: Journal of Agrotechnology Innovation, 4(2), 16–19. Retrieved from https://jurnal.ugm.ac.id/Agrinova/ DOI: https://doi.org/10.22146/a.77010
Yemiş, F., & Harmancı, N. Y. (2020). Classification, uses and environmental implications of disinfectants. Pakistan Journal of Analytical and Environmental Chemistry, 21(2), 179–192. https://doi.org/10.21743/pjaec/2020.12.20 DOI: https://doi.org/10.21743/pjaec/2020.12.20
Zahara, M. (2017). A review: micropropagation of phalaenopsis sp from leaf and flower stalk explants. Jurnal Natural, 17(2), 91–95. DOI: https://doi.org/10.24815/jn.v17i2.8130
Zavira, N., & Sari, S. Y. (2023). Validitas instrumen aktivitas belajar siswa dalam pembelajaran fisika. ARZUSIN, 3(6), 737–749. https://doi.org/10.58578/arzusin.v3i6.1940 DOI: https://doi.org/10.58578/arzusin.v3i6.1940
Zhao, X., Yu, S., Wang, Y., Jiang, D., Zhang, Y., Hu, L., … Cai, X. (2023). Field performance of disease-free plants of ginger produced by tissue culture and agronomic, cytological, and molecular characterization of the morphological variants. Agronomy, 13(1), 74. https://doi.org/10.3390/agronomy13010074 DOI: https://doi.org/10.3390/agronomy13010074
Zuzarte, M., Salgueiro, L., & Canhoto, J. (2024). Plant tissue culture: industrial relevance and future directions. In Plants as Factories for Bioproduction: Recent Developments and Applications (pp 1-15). Springer. https://doi.org/10.1007/10_2024_254 DOI: https://doi.org/10.1007/10_2024_254
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