Effectiveness of Herbs and Spices as Natural Antioxidants in Preserving Tuna (Thunnus sp.) and Nile Tilapia (Oreochromis niloticus): Enhancing Microbial Safety and Shelf Life

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DOI:

10.29303/jppipa.v11i11.12802

Published:

2025-11-25

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Abstract

Fish is a protein-rich and highly nutritious food. Natural herbs and spices contain antioxidant and antibacterial compounds that may enhance microbiological safety and extend shelf life. This study aimed to evaluate natural herb and spice-based processing methods for preserving tuna (Thunnus sp.) and nile tilapia (Oreochromis niloticus), along with the effectiveness of specific spices in reducing oxidation and microbiological contamination. Two marination approaches were applied, using both uncooked and cooked spice mixtures. Three recipes were prepared, recipe 1: garlic, pepper, salt, turmeric, coriander, lemongrass, recipe 2: garlic, pepper, salt, rosemary, oregano, thyme, lemon and recipe 3: garlic, pepper, salt, coriander, lemongrass, chili pepper, curry leaves, asam sunti. Unmarinated fish served as the control. All samples were vacuum-packed in retort pouches and stored at 1-4 °C for 1 h, 3, 5, and 7 days. Microbial analysis included total aerobic bacteria, Escherichia coli, Coliforms, Salmonella spp., Shigella spp., yeast and mold. Marination with herbs and spices significantly reduced microbial contamination compared to the control. The combined use of herbs and spices effectively improved microbial safety and extended the shelf life of tuna and nile tilapia. These findings support the application of herbs and spices as natural preservation agents in fish products, offering a safer and more sustainable alternative in fish preservation.

Keywords:

Antioxidant, Herbs, Nile, Preservation, Spices, Tuna

References

Abdalla, T., Al-Rumaithi, H., Osaili, T. M., Hasan, F., Obaid, R. S., Abushelaibi, A., & Ayyash, M. M. (2022). Prevalence, Antibiotic-Resistance, and Growth Profile of Vibrio spp. Isolated From Fish and Shellfish in Subtropical-Arid Area. Frontiers in Microbiology, 13(April). https://doi.org/10.3389/fmicb.2022.861547

Akinjogunla, O. J., Inyang, C. U., & Akinjogunla, V. F. (2011). Bacterial species associated with anatomical parts of fresh and smoked Bonga fish (Ethmalosa fimbriata): Prevalence and susceptibility to cephalosporins. Research Journal of Microbiology, 6(1), 87–97. https://doi.org/10.3923/jm.2011.87.97

Akpoghelie, P. O., Edo, G. I., Kasar, K. A., Zainulabdeen, K., Yousif, E., Mohammed, A. A., Jikah, A. N., Ezekiel, G. O., Owheruo, J. O., Ugbune, U., Umar, H., Ekokotu, H. A., Oghroro, E. E. A., Onyibe, P. N., Ekpekpo, L. D., Isoje, E. F., & Agbo, J. J. (2024). Impact of different nitrogen sources, initial pH and varying inoculum size on the fermentation potential of Saccharomyces cerevisiae on wort obtained from sorghum substrate. Food Materials Research, 4. https://doi.org/10.48130/fmr-0024-0012

Amit, S. K., Uddin, M. M., Rahman, R., Islam, S. M. R., & Khan, M. S. (2017). A review on mechanisms and commercial aspects of food preservation and processing. Agriculture and Food Security, 6(1), 1–22. https://doi.org/10.1186/s40066-017-0130-8

Antunes, P., Campos, J., Mourão, J., Pereira, J., Novais, C., & Peixe, L. (2018). Inflow water is a major source of trout farming contamination with Salmonella and multidrug resistant bacteria. Science of the Total Environment, 642, 1163–1171. https://doi.org/10.1016/j.scitotenv.2018.06.143

Banhidi, M. (2001). pH and ORP. Metal Finish, 99(1), 593-599. https://doi.org/10.1016/S0026-0576(01)85317-4

Bieżanowska-Kopeć, R., & Piatkowska, E. (2022). Total Polyphenols and Antioxidant Properties of Selected Fresh and Dried Herbs and Spices. Applied Sciences (Switzerland), 12(10). https://doi.org/10.3390/app12104876

BPOM. (2012). Badan Standarisasi Nasional. In Badan Pengawas Obat Dan Makanan Republik Indonesia. https://standarpangan.pom.go.id/dokumen/pedoman/Buku_Pedoman_PJAS_tentang_Cemaran.pdf

Byrd, K. A., Thilsted, S. H., & Fiorella, K. J. (2021). Fish nutrient composition: A review of global data from poorly assessed inland and marine species. In Public Health Nutrition (Vol. 24, Issue 3, pp. 476–486). Cambridge University Press. https://doi.org/10.1017/S1368980020003857

Chamorro, F., Cassani, L., Garcia-Oliveira, P., Barral-Martinez, M., Jorge, A. O. S., Pereira, A. G., Otero, P., Fraga-Corral, M., P. P. Oliveira, M. B., & Prieto, M. A. (2024). Health benefits of bluefin tuna consumption: (Thunnus thynnus) as a case study. In Frontiers in Nutrition (Vol. 11). Frontiers Media SA. https://doi.org/10.3389/fnut.2024.1340121

Darwish, W. S., Mohamed, A. S. E., & Abdallah, K. M. E. (2023). Mould Contamination of Fish and Fish Products with a Special Reference to its Public Health Significance: A Review. Journal of Advanced Veterinary Research, 13(8), 1726–1729. Retrieved from https://www.advetresearch.com/index.php/AVR/article/view/1487

Dib, A. L., Agabou, A., Chahed, A., Kurekci, C., Moreno, E., Espigares, M., & Espigares, E. (2018). Isolation, molecular characterization and antimicrobial resistance of enterobacteriaceae isolated from fish and seafood. Food Control, 88, 54–60. https://doi.org/10.1016/j.foodcont.2018.01.005

Dixon, W. R., Watts, E. G., King, J. A., Fu, X., & Wicker, L. (2020). Shelf-Stable Sustainable Shrimp Thermally Processed With Reciprocal Agitation. Frontiers in Sustainable Food Systems, 4(October), 1–12. https://doi.org/10.3389/fsufs.2020.569790

Eden, R. (2014). Enterobacteriaceae, Coliforms and E.Coli: Classical and Modern Methods for Detection and Enumeration. In Encyclopedia of Food Microbiology: Second Edition (Second Edi, Vol. 1). Elsevier. https://doi.org/10.1016/B978-0-12-384730-0.00097-5

Faheem, F., Liu, Z. W., Rabail, R., Haq, I. U., Gul, M., Bryła, M., Roszko, M., Kieliszek, M., Din, A., & Aadil, R. M. (2022). Uncovering the Industrial Potentials of Lemongrass Essential Oil as a Food Preservative: A Review. Antioxidants, 11(4), 1–21. https://doi.org/10.3390/antiox11040720

Fitri, N., Chan, S. X. Y., Che Lah, N. H., Jam, F. A., Misnan, N. M., Kamal, N., Sarian, M. N., Mohd Lazaldin, M. A., Low, C. F., Hamezah, H. S., Rohani, E. R., Mediani, A., & Abas, F. (2022). A Comprehensive Review on the Processing of Dried Fish and the Associated Chemical and Nutritional Changes. In Foods (Vol. 11, Issue 19). MDPI. https://doi.org/10.3390/foods11192938

Ghaly, A. E., Dave, D., Budge, S., & Brooks, M. S. (2010). Fish Spoilage Mechanisms and Preservation Techniques: Review. American Journal of Applied Sciences, 7(7), 859–877. https://doi.org/10.3844/ajassp.2010.859.877

Golden, C. D., Koehn, J. Z., Shepon, A., Passarelli, S., Free, C. M., Viana, D. F., Matthey, H., Eurich, J. G., Gephart, J. A., Fluet-Chouinard, E., Nyboer, E. A., Lynch, A. J., Kjellevold, M., Bromage, S., Charlebois, P., Barange, M., Vannuccini, S., Cao, L., Kleisner, K. M., … Thilsted, S. H. (2021). Aquatic foods to nourish nations. Nature, 598(7880), 315–320. https://doi.org/10.1038/s41586-021-03917-1

Gómez, I., Janardhanan, R., Ibañez, F. C., & Beriain, M. J. (2020). The effects of processing and preservation technologies on meat quality: Sensory and nutritional aspects. In Foods (Vol. 9, Issue 10). MDPI AG. https://doi.org/10.3390/foods9101416

Indio, V., Savini, F., Gardini, F., Barbieri, F., Prandini, L., Mekonnen, Y. T., Tomasello, F., Giacometti, F., Seguino, A., Serraino, A., & De Cesare, A. (2024). Microbiological safety of dry-cured fish from the raw material to the end of processing. International Journal of Food Microbiology, 415(February). https://doi.org/10.1016/j.ijfoodmicro.2024.110641

Jiménez-Ruíz, E. I., Ocaño-Higuera, V. M., Valdez-Hurtado, S., Cruz-Guzmán, J. A., Otero-León, C. B., Ruíz-Cruz, S., Garzón-García, A. M., Barrales-Cureño, H. J., Canizales-Rodríguez, D. F., Pérez-Martínez, C. J., & Sumaya-Martínez, M. T. (2023). Quality Improvement and Shelf-Life Extension of Iced Nile Tilapia Fillets Using Natural Garlic Extract. Fishes, 8(6), 1–15. https://doi.org/10.3390/fishes8060325

Jirna, I. N., Sudarmanto, I. G., Kurniawan, S. B., Ratih, G. A. M., & Rasyid, B. (2020). The potential of traditional balinese spices against the growth of Salmonella sp in vitro. Jurnal Teknologi Laboratorium, 9(1), 121–127. https://doi.org/10.29238/teknolabjournal.v9i1.200

Juglal, S., Govinden, R., & Odhav, B. (2002). Spice oils for the control of co-occurring mycotoxin-producing fungi. Journal of Food Protection, 65(4), 683–687. https://doi.org/10.4315/0362-028X-65.4.683

Kačániová, M., Čmiková, N., Vukovic, N. L., Verešová, A., Bianchi, A., Garzoli, S., Ben Saad, R., Ben Hsouna, A., Ban, Z., & Vukic, M. D. (2024). Citrus limon Essential Oil: Chemical Composition and Selected Biological Properties Focusing on the Antimicrobial (In Vitro, In Situ), Antibiofilm, Insecticidal Activity and Preservative Effect against Salmonella enterica Inoculated in Carrot. Plants, 13(4). https://doi.org/10.3390/plants13040524

Kurek, M., Pišonić, P., Ščetar, M., Janči, T., Čanak, I., Vidaček Filipec, S., Benbettaieb, N., Debeaufort, F., & Galić, K. (2024). Edible Coatings for Fish Preservation: Literature Data on Storage Temperature, Product Requirements, Antioxidant Activity, and Coating Performance—A Review. In Antioxidants (Vol. 13, Issue 11). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/antiox13111417

Lambrechts, K., & Rip, D. (2024). Listeria monocytogenes in the seafood industry: Exploring contamination sources, outbreaks, antibiotic susceptibility and genetic diversity. In MicrobiologyOpen (Vol. 13, Issue 5). John Wiley and Sons Inc. https://doi.org/10.1002/mbo3.70003

Liu, S., Fan, W., Zhong, S., Ma, C., Li, P., Zhou, K., Peng, Z., & Zhu, M. (2010). Quality evaluation of tray-packed tilapia fillets stored at 0°C based on sensory, microbiological, biochemical and physical attributes. African Journal of Biotechnology, 9(5), 692–701. https://doi.org/10.5897/ajb09.1369

Lopes, S. M., da Silva, D. C., & Tondo, E. C. (2022). Bactericidal effect of marinades on meats against different pathogens: a review. In Critical Reviews in Food Science and Nutrition (Vol. 62, Issue 27, pp. 7650–7658). Taylor and Francis Ltd. https://doi.org/10.1080/10408398.2021.1916734

Mafe, A. N., Edo, G. I., Makia, R. S., Joshua, O. A., Akpoghelie, P. O., Gaaz, T. S., Jikah, A. N., Yousif, E., Isoje, E. F., Igbuku, U. A., Ahmed, D. S., Essaghah, A. E. A., & Umar, H. (2024). A review on food spoilage mechanisms, food borne diseases and commercial aspects of food preservation and processing. Food Chemistry Advances, 5(November), 100852. https://doi.org/10.1016/j.focha.2024.100852

Magalhães, D., Vilas-Boas, A. A., Teixeira, P., & Pintado, M. (2023). Functional Ingredients and Additives from Lemon by-Products and Their Applications in Food Preservation: A Review. In Foods (Vol. 12, Issue 5). MDPI. https://doi.org/10.3390/foods12051095

Mapeka, T., Sandasi, M., Ncube, E., Viljoen, A., & van Vuuren, S. (2024). Enhancing the antimicrobial efficacy of common herbs and spices through an optimized polyherbal approach. South African Journal of Botany, 164, 91–99. https://doi.org/10.1016/j.sajb.2023.11.030

Misrahanum, M., Ayuningrum, N., & Helwati, H. (2022). Uji Fitokimia Dan Aktivitas Asam Sunti (Averrhoa bilimbi L.) Sebagai Antimikroba. Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi Dan Kesehatan, 7(1), 155–164. https://doi.org/10.36387/jiis.v7i1.854

Mulyati, S., Raharjo, M., & Nurjazuli. (2024). Factors Associated with the Presence of E.coli in Food: Literature Review. Media Publikasi Promosi Kesehatan Indonesia, 7(7), 1804–1810. https://doi.org/10.56338/mppki.v7i7.5325

Novak Babič, M., Gostinčar, C., & Gunde-Cimerman, N. (2020). Microorganisms populating the water-related indoor biome. Applied Microbiology and Biotechnology, 104(15), 6443–6462. https://doi.org/10.1007/s00253-020-10719-4

Odo, E. O., Ikwuegbu, J. A., Obeagu, E. I., Chibueze, S. A., & Ochiaka, R. E. (2023). Analysis of the antibacterial effects of turmeric on particular bacteria. Medicine (United States), 102(48), E36492. https://doi.org/10.1097/MD.0000000000036492

Olivas-Méndez, P., Chávez-Martínez, A., Santellano-Estrada, E., Asorey, L. G., Sánchez-Vega, R., Rentería-Monterrubio, A. L., Chávez-Flores, D., Tirado-Gallegos, J. M., & Méndez-Zamora, G. (2022). Antioxidant and Antimicrobial Activity of Rosemary (Rosmarinus officinalis) and Garlic (Allium sativum) Essential Oils and Chipotle Pepper Oleoresin (Capsicum annum) on Beef Hamburgers. Foods, 11(14). https://doi.org/10.3390/foods11142018

Oluchi, J., Amarachi, G., Chinyere, C., Chukwuma, C., Charles, O., Onyedikachi, V., Angus, N., Ebisomu, C., & Iheanyi, O. (2025). Antimicrobial Activity of Curry Leaf (Murraya keonigii) Extracts: An In-Vitro Study. In Covenant Journal Of Physical And Life Sciences (Vol. 13, Issue 1). Retrieved from https://ssrn.com/abstract=5209097

Parmar, B. K., Mohite, A. S., Pathan, D. I., Desai, A. S., & Wasave, S. M. (2024b). The use of herbs and spices in fish preservation at chilled temperature storage: opportunities and challenges. International Journal of Food Science and Technology, 59(10), 6758–6768. https://doi.org/10.1111/ijfs.17372

Pop, A., Muste, S., Paucean, A., Chis, S., Man, S., Salanta, L., Marc, R., Muresan, A., & Martis, G. (2019). Herbs and Spices in Terms of Food Preservation and Shelf Life. Hop and Medicinal Plants, 27(1–2), 57–65. https://doi.org/10.15835/hpm.v27i1-2.13585

Porto, Y. D., Fogaça, F. H. dos S., Andrade, A. O., da Silva, L. K. S., Lima, J. P., da Silva, J. L., Vieira, B. S., Cunha Neto, A., Figueiredo, E. E. de S., & Tassinari, W. de S. (2023). Salmonella spp. in Aquaculture: An Exploratory Analysis (Integrative Review) of Microbiological Diagnoses between 2000 and 2020. In Animals (Vol. 13, Issue 1). MDPI. https://doi.org/10.3390/ani13010027

Reis, J. O., Cavalcante, C. B., Nunes, N. B., Neto, A. C., Machado, M. A. M., Porto, Y. D., Castro, V. S., & Figueiredo, E. E. de S. (2024). Influence of Organic Matter from Native Fish on the Antimicrobial Efficacy of Sodium Hypochlorite (NaClO) in Reducing Salmonella spp. Population. Microbiology Research, 15(1), 342–353. https://doi.org/10.3390/microbiolres15010023

Reni Yuslianti, E., Susanto, A., Sutjiatmo, A. B., Widowati, W., Ayuni, V., & Hadiprasetyo, D. S. (2025). Antimicrobial and Antioxidant Activities of Black Cumin Seed (Nigella sativa) Ethanol Extract. HAYATI Journal of Biosciences, 32(5), 1273–1282. https://doi.org/10.4308/hjb.32.5.1273-1282

Romulo, A., Anjani, V. S., & Wardana, A. A. (2024). Enhancing Antimicrobial Activity of Thyme Essential Oil Through Cellulose Nano Crystals-Stabilized Pickering Emulsions. Foods, 13(22). https://doi.org/10.3390/foods13223706

Sanjee, S. Al, & Karim, M. E. (2016). Microbiological quality assessment of frozen fish and fish processing materials from Bangladesh. International Journal of Food Science, 2016. https://doi.org/10.1155/2016/8605689

Sengupta, S., Pradhan, A., Biswas, S., & Bhattacharya, M. (2024). Exploring the Antimicrobial Properties of Lemon: A Comparative Analysis of Peel, Seed, and Pulp. Microbiology Research Journal International, 34(9), 10–24. https://doi.org/10.9734/mrji/2024/v34i91477

Setyobudi, E., Murwantoko, Utami, A. M. R., & Syarifah, R. F. (2023). Anisakid nematodes from the largehead hairtail fish (Trichiurus lepturus) from the northern coast of Java, Indonesia. Biodiversitas, 24(3), 1560–1568. https://doi.org/10.13057/biodiv/d240328

Stevens, G. A., Beal, T., Mbuya, M. N. N., Luo, H., Neufeld, L. M., Addo, O. Y., Adu-Afarwuah, S., Alayón, S., Bhutta, Z., Brown, K. H., Jefferds, M. E., Engle-Stone, R., Fawzi, W., Hess, S. Y., Johnston, R., Katz, J., Krasevec, J., McDonald, C. M., Mei, Z., … Young, M. F. (2022). Micronutrient deficiencies among preschool-aged children and women of reproductive age worldwide: a pooled analysis of individual-level data from population-representative surveys. The Lancet Global Health, 10(11), e1590–e1599. https://doi.org/10.1016/S2214-109X(22)00367-9

Suleman, R., Zahoor, M. A., Qarni, M. A., Saleh, I. A., Rao, W., Hussain, M., Ismail, T., Akhtar, S., Okla, M. K., Alaraidh, I. A., AbdElgayed, G., Saud, S., Hassan, S., Nawaz, T., & Fahad, S. (2025). Assessment of heavy metals and microbial loads in Nile tilapia (Oreochromis niloticus) from different farms and rivers. Scientific Reports, 15(1), 1–11. https://doi.org/10.1038/s41598-025-87152-y

Syaifurrisal, A., Suciyono, Santanumurti, M. B., Prajitno, A., Fadjar, M., Riyadi, F. M., Fauziyyah, A. I., Pariawan, A., & Abualreesh, M. H. (2024). Effect of Curry Leaf (Murraya koenigii) as Edwardsiellosis Treatment on Gourami Fish (Osphronemus goramy). Aquatic Sciences and Engineering, 39(3), 179–188. https://doi.org/10.26650/ASE20231367517

Tahiluddin, A., Marıbao, I., Amlani, M., & Sarri, J. (2022). A Review on Spoilage Microorganisms in Fresh and Processed Aquatic Food Products. Food Bulletin, 1(1), 21–36. https://doi.org/10.29329/foodb.2022.495.05

Talebi, S. M., Naser, A., & Ghorbanpour, M. (2024). Chemical composition and antimicrobial activity of the essential oils in different populations of Coriandrum sativum L. (coriander) from Iran and Iraq. Food Science and Nutrition, 12(6), 3872–3882. https://doi.org/10.1002/fsn3.4047

Tymczewska, A., Klebba, J., & Szydłowska-Czerniak, A. (2023). Antioxidant Capacity and Total Phenolic Content of Spice Extracts Obtained by Ultrasound-Assisted Extraction Using Deep Eutectic and Conventional Solvents. Applied Sciences (Switzerland), 13(12). https://doi.org/10.3390/app13126987

Utomo, A. D., Aida, S. N., Wulandari, T. N. M., Zaidan, M., Ditya, Y. C., Agustini, S., Fatah, K., Ali, M., & Gribaldi. (2025). Growth, mortality, and fish stock of dominant fish species in Pondok Reservoir, East Java, Indonesia. Egyptian Journal of Aquatic Research. https://doi.org/10.1016/j.ejar.2025.05.007

Walasek-Janusz, M., Grzegorczyk, A., Malm, A., Nurzyńska-Wierdak, R., & Zalewski, D. (2024). Chemical Composition, and Antioxidant and Antimicrobial Activity of Oregano Essential Oil. Molecules, 29(2). https://doi.org/10.3390/molecules29020435

Widhianata, H., Basunanda, P., Supriyadi, S., & Taryono, T. (2022). Transcriptional Comparison of New Hybrid Progenies and Clone-Cultivars of Tea (Camellia sinensis L.) Associated to Catechins Content. Plants, 11(15). https://doi.org/10.3390/plants11151972

Zhao, K., Wonta, K. B., Xia, J., Zhong, F., & Sharma, V. (2024). Phytochemical profiling and evaluation of antimicrobial activities of common culinary spices: Syzygium aromaticum (clove) and Piper nigrum (black pepper). Frontiers in Nutrition, 11(August), 1–9. https://doi.org/10.3389/fnut.2024.1447144

Author Biographies

Hani Widhianata, Universitas Kristen Cipta Wacana

Arinda Eka Lidiastuti, Universitas Kristen Cipta Wacana Malang

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Widhianata, H., & Lidiastuti, A. E. (2025). Effectiveness of Herbs and Spices as Natural Antioxidants in Preserving Tuna (Thunnus sp.) and Nile Tilapia (Oreochromis niloticus): Enhancing Microbial Safety and Shelf Life. Jurnal Penelitian Pendidikan IPA, 11(11), 265–278. https://doi.org/10.29303/jppipa.v11i11.12802