Antioxidant Effectiveness and Phenolic Content of Saga Seed (Adenanthera pavonina) Extracts In Vitro Using DPPH and ABTS Methods
DOI:
10.29303/jppipa.v12i3.14293Published:
2026-03-25Downloads
Abstract
Oxidative stress plays a crucial role in the development of various degenerative diseases, highlighting the need for safe and effective natural antioxidants. This study aimed to evaluate the antioxidant activity of saga seed (Adenanthera pavonina) extracts using DPPH and ABTS assays and to examine their relationship with total phenolic content (TPC). An in vitro experimental study with a quantitative approach was conducted. The extraction process was performed using maceration with 95% methanol and 95% ethanol as solvents. Antioxidant activity was determined based on IC₅₀ values, while TPC was analyzed using the Folin–Ciocalteu method and expressed as mg gallic acid equivalents (GAE)/g extract. The results demonstrated that the methanolic extract exhibited stronger antioxidant activity (IC₅₀ DPPH = 86.43 ppm; IC₅₀ ABTS = 54.07 ppm) compared to the ethanolic extract (IC₅₀ DPPH = 99.11 ppm; IC₅₀ ABTS = 72.41 ppm). The methanolic extract also showed a higher TPC value (319.90 mg GAE/g) than the ethanolic extract (194.33 mg GAE/g).
Keywords:
ABTS Antioxidants DPPH method Phenolic content Saga seedsReferences
Asma, S. T., Acaroz, U., Imre, K., Morar, A., Shah, S. R. A., Hussain, S. Z., Arslan-Acaroz, D., Demirbas, H., Hajrulai-Musliu, Z., Istanbullugil, F. R., Soleimanzadeh, A., Morozov, D., Zhu, K., Herman, V., Ayad, A., Athanassiou, C., & Ince, S. (2022). Natural Products/Bioactive Compounds as a Source of Anticancer Drugs. Cancers, 14(24), 6203. https://doi.org/10.3390/cancers14246203 DOI: https://doi.org/10.3390/cancers14246203
Domínguez-Arca, V., Hellweg, T., & Antelo, L. T. (2025). Harnessing Thalassochemicals: Marine Saponins as Bioactive Agents in Nutraceuticals and Food Technologies. Marine Drugs, 23(6), 227. https://doi.org/10.3390/md23060227 DOI: https://doi.org/10.3390/md23060227
Dwitanti, N., Batubara, R., Julianti, E., Hanum, L., & Syahputri, W. (2020). Saga (Adenanthera pavonina Linn) Seeds Milk As An Alternative Source Of Protein From Tree Species. Journal of Sylva Indonesiana, 3(02), 78–86. https://doi.org/10.32734/jsi.v3i02.4283 DOI: https://doi.org/10.32734/jsi.v3i02.4283
EPIC. (2023). Air Quality Life Index: 2023 Annual Update. Energy Policy Institute at the University OfChicago.
García-Cervantes, A. M., Prates, J. A. M., & Guil-Guerrero, J. L. (2025). Overview of Primary and Secondary Metabolites of Rugulopteryx okamurae Seaweed: Assessing Bioactivity, Scalability, and Molecular Mechanisms. Marine Drugs, 23(9), 351. https://doi.org/10.3390/md23090351 DOI: https://doi.org/10.3390/md23090351
Kurniawati, I. F., & Sutoyo, S. (2021). Review artikel: potensi bunga tanaman sukun (artocarpus altilis [park. I] fosberg) sebagai bahan antioksidan alami. Unesa Journal of Chemistry, 10(1), 1–11. https://doi.org/10.26740/ujc.v10n1.p1-11 DOI: https://doi.org/10.26740/ujc.v10n1.p1-11
Muflihah, Y. M., Gollavelli, G., & Ling, Y.-C. (2021). Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants, 10(10), 1530. https://doi.org/10.3390/antiox10101530 DOI: https://doi.org/10.3390/antiox10101530
Mujahid, M., Siddiqui, H. H., Hussain, A., & Hussain, M. S. (2013). Hepatoprotective effects of Adenanthera pavonina (Linn.) against anti-tubercular drugs-induced hepatotoxicity in rats. Pharmacognosy Journal, 5(6), 286–290. https://doi.org/10.1016/j.phcgj.2013.08.003 DOI: https://doi.org/10.1016/j.phcgj.2013.08.003
Owoeye, T. F., Akinlabu, K. D., & Ajani, O. O. (2023). Proximate composition, phytochemical screening and mineral content studies of leaves extract of Adenanthera pavonina. Arab Journal of Basic and Applied Sciences, 30(1), 317–328. https://doi.org/10.1080/25765299.2023.2215437 DOI: https://doi.org/10.1080/25765299.2023.2215437
Putri, J. Y., Nastiti, K., & Hidayah, N. (2023). Pengaruh Pelarut Etanol 70% Dan Metanol Terhadap Kadar Flavonoid Total Ekstrak Daun Sirsak (Annona muricata Linn). Journal Pharmaceutical Care and Sciences, 3(2), 20–29. https://doi.org/10.33859/jpcs.v3i2.235 DOI: https://doi.org/10.33859/jpcs.v3i2.235
Rahmawati, D., Lestari, D., Agita, M., Salsabila, A. P., Lestari, Y. F., Salsabila, F., & Alfin, E. (2026). Pengamatan Tumbuhan Di Kebun Raya Cibinong. Jurnal Abdimas Indonesia, 6(1), 191–199. https://doi.org/10.34697/jai.v6i1.3040
Ramadhani, M. A., & Novema, A. P. (2022). Aktivitas antibakteri ekstrak kasar dan terpurifikasi daun cengkeh (Syzygium aromaticum) terhadap Escherichia coli dan Staphylococcus aureus. Borobudur Pharmacy Review, 2(1), 8–14. https://doi.org/10.31603/bphr.v2i1.6934 DOI: https://doi.org/10.31603/bphr.v2i1.6934
Ramzan, M. M. (2025). Phytochemical And Physicochemical Investigation Of Miraculous Oil From Seeds Of Adenanthera Pavonina L. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 236–249. https://doi.org/10.53555/hd1yze96 DOI: https://doi.org/10.53555/hd1yze96
Sari, A. A., Wulandari, F., & Adiningsih, M. U. (2025). Antioxidant Activity, Total Phenolic Content, and Total Flavonoid Content of Methanol Extract of Vernonia elaeagnifolia Leaves Using ABTS and DPPH Assays. Pharmacon: Jurnal Farmasi Indonesia, 144–150. https://doi.org/10.23917/pharmacon.v22i2.13498 DOI: https://doi.org/10.23917/pharmacon.v22i2.13498
Sundarasamy, A., Senniappan, T. S., & Thangaraj, S. (2022). An updated Review on Phytochemical and Pharmacological Studies of Adenanthera pavonina Linn. Current Trends in Biotechnology and Pharmacy, 16, 153–162. https://doi.org/10.5530/ctbp.2022.2s.41
Susilowati, A., Ahmad, A. G., Rangkuti, A. B., Harahap, M. M., Sujarwo, & Ginting, I. M. (2021). The health status of saga tree (Adenanthera pavonina) in campus area of Universitas Sumatera Utara (USU). IOP Conference Series: Earth and Environmental Science, 782(4), 042029. https://doi.org/10.1088/1755-1315/782/4/042029 DOI: https://doi.org/10.1088/1755-1315/782/4/042029
Widiasriani, I. A. P., Udayani, N. N. W., Triansyah, G. A. P., Dewi, N. P. E. M. K., Wulandari, N. L. W. E., & Prabandari, A. A. S. S. (2024). Artikel Review: Peran Antioksidan Flavonoid dalam Menghambat Radikal Bebas. Journal Syifa Sciences and Clinical Research, 6(2), 188–197. https://doi.org/10.37311/jsscr.v6i2.27055 DOI: https://doi.org/10.37311/jsscr.v6i2.27055
Wijesekara, M. A., Goonerathne, L. V., Soysa, P., Perera, D. B., Jayasena, S., Jayasiri, A., & Kottahachchi, D. U. (2024). In vitro Screening of Antioxidant and Anti-inflammatory Activities of Plant Extract Adenanthera pavonina. Proceedings of International Forestry and Environment Symposium, 27. https://doi.org/10.31357/fesympo.v27.7107 DOI: https://doi.org/10.31357/fesympo.v27.7107
World Health Organization. (2021). WHO global air quality guidelines: particulate matter (PM2.5 and PM10), ozone, nitrogen dioxide, sulfur dioxide and carbon monoxide. Retrieved from https://www.who.int/publications/i/item/9789240034228
Yumita, A., Hanani, E., Agustina, A., Damayanti, F., Priani, K. N., & Fadila, S. N. (2023). Total Phenolic Content and Antioxidant Activities of Leaves and Bark Extract of Adenanthera pavonina L. Natural Product Sciences, 29(1), 24–30. https://doi.org/10.20307/nps.2023.29.1.24 DOI: https://doi.org/10.20307/nps.2023.29.1.24
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