Fingerprinting Compound Profile of Tammate (Lannea coromandelica (Hout) Merr.) Bark Extract Using Chemometric and FTIR Analysis
DOI:
10.29303/jppipa.v12i5.14579Published:
2026-05-25Downloads
Abstract
Lannea coromandelica is a traditional medicinal plant rich in alkaloids, flavonoids, saponins, and tannins with reported antioxidant, antimicrobial, anti-inflammatory, antidiabetic, and antirheumatic activities. Previous studies have primarily emphasized phytochemical screening and pharmacological evaluation, whereas metabolite fingerprint characterization using spectroscopic and chemometric approaches remains limited. This study aimed to characterize the metabolite fingerprint profile of L. coromandelica stem bark extracts using Fourier Transform Infrared (FTIR) spectroscopy and chemometric analysis. Twelve extracts were prepared using maceration, Reflux and sonication with distilled water, ethanol, n-hexane, and ethyl acetate to obtain metabolites with different polarities. FTIR spectroscopy was employed due to its rapid, non-destructive, and reproducible detection of functional groups, while chemometric analysis enhanced extract discrimination and pattern recognition. FTIR spectra revealed O–H, N–H, C–H, C=C, C=O, and C–C functional groups associated with phenolic, aromatic, and alkaloidal compounds related to antioxidant and anti-inflammatory activities. Principal Component Analysis (PCA) showed clear clustering patterns influenced by extraction method and solvent polarity, while Cluster Analysis (CA) demonstrated similarity values of 35.54–99.82% among extracts. These findings confirm that FTIR combined with chemometrics is effective for metabolite fingerprint characterization and quality standardization of L. coromandelica extracts.
Keywords:
Chemometric Fingerprinting compound FTIR Lannea coromandelica (Houtt.) MerrReferences
Agung, T. S., Zuhri, B. S. S., & Auvaria, S. W. (2024). Hylopoly-Bag: Environmentally Friendly Bioplastic Innovation Made from Dragon Fruit (Hylocereus polarizes) Peel Waste to Support Golden Indonesia 2045. Jurnal Penelitian Pendidikan IPA, 10(10). https://doi.org/10.29303/jppipa.v10i10.7703
Ananda, K. R., Muthmainah, & Hikmayani, N. H. (2025). Kelakai (Stenochlaena palustris (Burm.) Bedd) Extract from Kalimantan: Determination of Total Phenol and Alkaloid Content Potential as Nutraceuticals. Jurnal Penelitian Pendidikan IPA, 11(1). https://doi.org/10.29303/jppipa.v11i1.9786
AR, Muh. A., Hikma, N., Yasir, B., Asril Burhan, A., & Khairuddin, K. (2023). Optimization of Extraction Lannea coromandelica for Antioxidant Activity using Analytical Factorial Design Approach. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 9(2). https://doi.org/10.22487/j24428744.2023.v9.i2.16106
Cao, Z., Wang, Z., Shang, Z., & Zhao, J. (2017). Classification and identification of Rhodobryum roseum limpr. And its adulterants based on fourier-transform infrared spectroscopy (FTIR) and chemometrics. PLoS ONE, 12(2). https://doi.org/10.1371/journal.pone.0172359
Chaves, J. O., de Souza, M. C., da Silva, L. C., Lachos-Perez, D., Torres-Mayanga, P. C., Machado, A. P. da F., Forster-Carneiro, T., Vázquez-Espinosa, M., González-de-Peredo, A. V., Barbero, G. F., & Rostagno, M. A. (2020). Extraction of Flavonoids From Natural Sources Using Modern Techniques. In Frontiers in Chemistry (Vol. 8). https://doi.org/10.3389/fchem.2020.507887
Dhea, L. A., Kurniawan, A., Ulfa, S. M., & Karimah, K. (2023). Correlation of Microplastic Size Distribution and Water Quality Parameters in the Upstream Brantas River. Jurnal Penelitian Pendidikan IPA, 9(2). https://doi.org/10.29303/jppipa.v9i2.2777
Dmitrienko, S. G., Apyari, V. V., Tolmacheva, V. V., Gorbunova, M. V., Furletov, A. A., & Zolotov, Y. A. (2024). Methods for the Extraction of Organic Compounds from Solid Samples: 1. Solvent Extraction. Review of Reviews. Journal of Analytical Chemistry, 79(8). https://doi.org/10.1134/S1061934824700382
Farnsworth, W. R. (2016). Chemometrics and Intelligent Laboratory Systems Award. In Chemometrics and Intelligent Laboratory Systems, 157. https://doi.org/10.1016/j.chemolab.2016.08.009
Hssaini, L., Elfazazi, K., Razouk, R., Ouaabou, R., Hernandez, F., Hanine, H., Charafi, J., Houmanat, K., & Aboutayeb, R. (2021). Combined effect of cultivar and peel chromaticity on figs’ primary and secondary metabolites: Preliminary study using biochemical and ftir fingerprinting coupled to chemometrics. Biology, 10(7). https://doi.org/10.3390/biology10070573
Idawati, S., Hakim, A., & Andayani, Y. (2019). Pengaruh Metode Isolasi a-mangostin dari Kulit Buah Manggis (Garcinia mangostana L.) terhadap Rendemen α-mangostin. Jurnal Penelitian Pendidikan IPA, 5(2). https://doi.org/10.29303/jppipa.v5i2.167
Juariah, S., Juliansyah, R., & Wahyuni, N. (2025). Isolation and Antimicrobial Activity Test of n-Hexane of Pineapple Core Ananas comosus L. Merr. Jurnal Penelitian Pendidikan IPA, 11(3). https://doi.org/10.29303/jppipa.v11i3.10696
Khair, K., Andayani, Y., & Hakim, A. (2017). Identifikasi senyawa metabolit sekunder pada hasil fraksinasi ekstrak phaseolus vulgaris l. Dengan metode gas chromatography-mass spectroscopy (GC-MS). Jurnal Penelitian Pendidikan IPA, 3(1). https://doi.org/10.29303/jppipa.v3i1.51
Laksono, M. T., & Hayati, E. K. (2021). Analisis Sidik Jari Kromatografi Lapis Tipis Tanaman Anting-Anting (Acalypha indica L.). Alchemy, 9(2). https://doi.org/10.18860/al.v9i2.11613
Liu, X., Liu, X., Wang, J., Zang, D., Yang, Y., Chen, Q., & Guo, D. an. (2025). Machine learning and chemometric methods for high-throughput authentication of 53 Root and Rhizome Chinese Herbal using ATR-FTIR fingerprints. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1260. https://doi.org/10.1016/j.jchromb.2025.124630
Ma, Y., Zhang, Y., Kong, L., & Yang, J. (2018). Mechanoresponsive material of AIE-active 1,4-dihydropyrrolo[3,2-b]pyrrole luminophores bearing tetraphenylethylene group with rewritable data storage. Molecules, 23(12). https://doi.org/10.3390/molecules23123255
Mahmuda, S., Afendi, F. M., & Rafi, M. (2020). Kajian Statistika Data Spektrum FTIR Meniran (Phyllanthus niruri) Asal Pulau Jawa. Jurnal Statistika Teori Dan Aplikasi, 14(1). https://doi.org/10.1234/bias.v14i1.93
Mardin, S. (2025). Repellent Bioactivity of Lantana camara Leaf Extract Against Culex Mosquitoes. Jurnal Penelitian Pendidikan IPA, 11(2). https://doi.org/10.29303/jppipa.v11i2.9586
Maruzy, A., Budiarti, M., & Subositi, D. (2020). Autentikasi Centella asiatica (L.) Urb. (Pegagan) dan Adulterannya Berdasarkan Karakter Makroskopis, Mikroskopis, dan Profil Kimia. Jurnal Kefarmasian Indonesia. https://doi.org/10.22435/jki.v10i1.1830
Marwulan, M., Nursyam, H., & Kilawati, Y. (2023). Antibacterial Activity Test of Tembelekan Leaf Extract (Lantana camara linn) against Edwardsiella tarda bacteria. Jurnal Penelitian Pendidikan IPA, 9(5). https://doi.org/10.29303/jppipa.v9i5.3459
Mutmainnah, P. A., Hakim, A., & Savalas, L. R. T. (2017). Identifikasi Senyawa Turunan Hasil Fraksinasi Kayu Akar Artocarpus Odoratissimus. Jurnal Penelitian Pendidikan IPA, 3(2). https://doi.org/10.29303/jppipa.v3i2.89
Noviana, E., Indrayanto, G., & Rohman, A. (2022). Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines. Frontiers in Pharmacology, 13. https://doi.org/10.3389/fphar.2022.853023
Osman, S. O. M., Saad, A. S. I., Tadano, S., Takeda, Y., Konaka, T., Yamasaki, Y., Tahir, I. S. A., Hisashi, T., & Akashi, K. (2022). Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy. International Journal of Molecular Sciences, 23(5). https://doi.org/10.3390/ijms23052842
Patle, T. K., Shrivas, K., Jamunkar, R., Kumar, A., & Tandey, K. (2025). Screening of phenolics and flavonoids using FTIR and UV-Vis: Antioxidant activity and HPLC quantification of gallic acid and ellagic acid. Journal of Ravishankar University (PART-B), 38(1). https://doi.org/10.52228/jrub.2025-38-1-11
Putra, B., & Rahman, S. (2020). Uji aktivitas ekstrak etil asetat daun kayu jawa (lannea coromandelic (houtt.) Merr.) Terhadap penyembuhan luka bakar pada tikus putih (Rattus norvegicus). As-Syifaa Jurnal Farmasi, 12(2), 158–163. Retrieved from https://jurnal.farmasi.umi.ac.id/index.php/as-syifaa/article/view/720/pdf
Rafi, M., Nomi, A. G., Septaningsih, D. A., Heryanto, R., & Putri, S. P. (2022). Quantitative HPLC and FTIR-based Metabolomics for Clustering Centella asiatica Cultivation Ages and Evaluation of Their Radical Scavenging Activity. Sains Malaysiana, 51(6). https://doi.org/10.17576/jsm-2022-5106-15
Socaciu, C., Fetea, F., Ranga, F., Bunea, A., Dulf, F., Socaci, S., & Pintea, A. (2020). Attenuated total reflectance‐fourier transform infrared spectroscopy (Atr‐ftir) coupled with chemometrics, to control the botanical authenticity and quality of cold‐pressed functional oils commercialized in romania. Applied Sciences (Switzerland), 10(23). https://doi.org/10.3390/app10238695
Syahrir, M., Azwar, Muh. A., Pabunga, C., & Yasir, B. (2025). Analisis Sidik Jari Variasi Metode Ekstraksi Kulit Batang Tanaman Tammate (Lannea coromandelica) Menggunakan Analisis Densitometri dan Kemometrik. Herbal Medicine Journal, 8(1), 13–21. Retrieved from https://hmj.jurnalsenior.com/index.php/hmj/article/view/141/113
Tampang, R., Alaydrus, S., Dewi, N. P., & Tandi, J. (2024). Determination of specific and non-specific standardization parameters for ethanol extract of purple leaves (Graptophyllum pictum (L) Griff). Jurnal Penelitian Pendidikan IPA, 10(9). https://doi.org/10.29303/jppipa.v10i9.8812
Triawan, D. A., Nurwidiyani, R., & Adfa, M. (2025). The performance of FTIR and UV-Vis spectroscopy combined with chemometrics to classify Impatiens balsamina L. leaf extracts from different solvent extraction. Vegetos. https://doi.org/10.1007/s42535-025-01246-6
Tulukcu, E., Cebi, N., & Sagdic, O. (2019). Chemical fingerprinting of seeds of some salvia species in Turkey by using GC-MS and FTIR. Foods, 8(4). https://doi.org/10.3390/foods8040118
Ulandari, A. S., & Sani, S. K. (2023). Identifikasi Senyawa Metabolit Sekunder Ekstrak Metanol Daun dan Kulit Batang Banten (Lannea coromandelica) Menggunakan GC-MS Sebagai Tanaman Obat. Jurnal Ilmu Kefarmasian, 4(1). https://doi.org/10.31764/lf.v4i1.11127
Umar, A. H., Syahruni, R., Burhan, A., Maryam, F., Amin, A., Marwati, & Masero, L. R. (2016). Determinasi dan analisis finger print tanaman murbei (Morus alba Lour) Sebagai bahan baku obat tradisional dengan metode spektroskopi FT-IR dan kemometrik. Pharmacon Jurnal Ilmiah Farmasi-UNSRAT, 5(1). https://doi.org/10.35799/pha.5.2016.11227
Wang, X., Liu, X., Wang, J., Wang, G., Zhang, Y., Lan, L., & Sun, G. (2021). Study on multiple fingerprint profiles control and quantitative analysis of multi-components by single marker method combined with chemometrics based on Yankening tablets. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 253. https://doi.org/10.1016/j.saa.2021.119554
Wang, Y. Y., Li, J. Q., Liu, H. G., & Wang, Y. Z. (2019). Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) combined with chemometrics methods for the classification of Lingzhi species. Molecules, 24(12). https://doi.org/10.3390/molecules24122210
Wong, K. C. (2015). Review of Spectrometric Identification of Organic Compounds. Journal of Chemical Education, 92(10). https://doi.org/10.1021/acs.jchemed.5b00571
Yasir, B., Azwar Ar, M., Burhan, A., Khairuddin, K., Alam, G., Irnawati, I., & Rohman, A. (2025). Chemometrics-assisted fingerprinting profiling of Mangosteen extracts from Sulawesi island using Fourier Transform Infrared (FTIR) Spectroscopy. Journal of Research in Pharmacy, 29(1). https://doi.org/10.12991/jrespharm.1628579
Yasir, B., Purwaningsih, D., Rumata, R., Wahyuddin, N., Ar, M. A., Hikmah, N., Rahman, F., & Hendrarti, W. (2021). Application Chemometrics-Assisted Fingerprinting Profiling of Extract Variation from Mangosteen (Garcinia mangostana L.) Using FTIR Method. Journal of Pharmaceutical and Medicinal Sciences, 6(2). Retrieved from https://garuda.kemdiktisaintek.go.id/documents/detail/3009943
Zahara, H., Karma, T., & Yusuf, M. (2023). Klasifikasi Daun Biduri (Calotropis Gigantea L.) Dari Lokasi Berbeda Menggunakan Spektroskopi Inframerah Dan Kemometrik. Lantanida Journal, 11(2). https://doi.org/10.22373/lj.v11i2.15096
Zulfani, E., Satria, D., Widya, N., & Rivaldo, I. (2025). Klasifikasi Tiga Varietas Daun Gambir (Uncaria Gambir Roxb.) Menggunakan Spektroskopi Inframerah dan Analisis Kemometrik. Menara Ilmu, 19(2). https://doi.org/10.31869/mi.v19i2.6658
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