Vol. 12 No. 5 (2026): In Progress
Open Access
Peer Reviewed

The Influence of Stub Length and Texture of Pruning Wound Surface on the Wound Occlusion (Case Study on Tree Healt at the Mataram University Campus)

Authors

Suripto , Lalu Muhammad Aby Dujana , Rachmawati Noviana Rahayu , Supardiono

DOI:

10.29303/jppipa.v12i5.14668

Published:

2026-05-25

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Abstract

Improper tree pruning, such as leaving long stubs and rough pruning wounds often leads to tree health problems in the form of poor wound occulision.  This study aimed to determine the effect of stub length and pruning wound texture on wound occlusion. Stub length was measured in cm, wound texture was scored as 0 if rough and 1 if smooth, and wound occlusion was measured as the % of wound surface area covered. The influence of stub length and wound texture was tested using regression analysis. The results showed that on short stubs (4-12 cm), smooth wounds produced good to very good occlusion, while rough wounds produced poor occlusion. As the stub length increases to 14–35 cm (medium stub), smooth wounds still produced rather to good occlusion. On long stubs (40-100 cm), maximum occlusion was only rather good  and predominantly poor. In very long stubs (>100 cm), occlusion was consistently poor to very poor, regardless of wound texture. Stub length and wound texture significantly influence wound occlusion with the model Y = 57.7283 -0.44X1 +13.03X2. To achieve good occlusion (>65%), the remaining stub length should be <14 cm with a smooth pruning wound.

Keywords:

Occlusion Pruning wound Stub Tree

References

Anwar, N., & Isda, M. N. (2021). Respons Pembentukan Kalus Daun Pegagan (Centella asiatica (L.) Urb.) dengan Penambahan Naphtalene Acetic Acid dan Benzyl Amino Purin Secara In Vitro. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 5(2), 136–142. https://doi.org/10.24002/biota.v5i3.3232

Avifah, S. N., Wulandari, A. S., Roostika, I., & Fauzan, Y. S. A. (2026). Callus Induction of Javanese Endemic Tectona grandis f. abludens. Jurnal Penelitian Pendidikan IPA, 12(1), 387–396. https://doi.org/10.29303/jppipa.v12i1.13142

Carluccio, G., Benigno, A., Panzavolta, T., Vergine, M., De Bellis, L., Luvisi, A., & Moricca, S. (2025). Understanding Oak Decline in Europe: Ecological Factors, Symptoms, Causative Agents, and Management Strategies. Plant Disease, 109(9), 1805–1823. https://doi.org/10.1094/PDIS-11-24-2401-FE

Clatterbuck, W. K. (2006). Tree Wounds: Response of Trees and What You Can Do. Department of Agriculture and County Governments Cooperating. Retrieved from https://utia.tennessee.edu/publications/wp-content/uploads/sites/269/2023/10/SP683.pdf

Cobb, R., & Metz, M. (2017). Tree Diseases as a Cause and Consequence of Interacting Forest Disturbances. Forests, 8(5), 147. https://doi.org/10.3390/f8050147

Das, A. J., Stephenson, N. L., & Davis, K. P. (2016). Why do trees die? Characterizing the drivers of background tree mortality. Ecology, 97(10), 2616–2627. https://doi.org/10.1002/ecy.1497

Ekawati, Y., Anggraeni, A., Dyah Prawestri, A., & Nurtjahya, E. (2022). Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro. AGROSAINSTEK: Jurnal Ilmu Dan Teknologi Pertanian, 6(2), 28–37. https://doi.org/10.33019/agrosainstek.v6i2.316

Ezzati, S., & Nasseri, M. H. (2025). Long-term prediction of wound closure in residual damaged trees using Markov chain analysis. Biosystems Engineering, 254, 104141. https://doi.org/10.1016/j.biosystemseng.2025.104141

Fazriati, E., Purnama, A., Algifari, G. A., Muslimah, I. S. A., Sumanto, F. H. P., Kirana, K. H., & Susanto, K. (2022). Cross-Section Resistivity Detection of Tree (Swietenia Magahoni and Gmelina) Using ERT Method. Jurnal Penelitian Pendidikan IPA, 8(1), 39–44. https://doi.org/10.29303/jppipa.v8i1.965

Fehér, A. (2019). Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? Frontiers in Plant Science, 10, 1–11. https://doi.org/10.3389/fpls.2019.00536

Feng, M., Zhang, A., Nguyen, V., Bisht, A., Almqvist, C., De Veylder, L., Carlsbecker, A., & Melnyk, C. W. (2024). A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing. Nature Plants, 10(1), 53–65. https://doi.org/10.1038/s41477-023-01568-w

Gauthier, N. W., Fountain, W. E., & Missun, T. (2015). Tree wounds: Invitations to wood decay fungi. Plant Pathology Fact Sheet, 7. Retrieved from https://plantpathology.ca.uky.edu/files/ppfs-or-w-01.pdf

Giannino, F., Hay Mele, B., De Micco, V., Toraldo, G., Mazzoleni, S., & Carteni, F. (2019). An Individual Based Model of Wound Closure in Plant Stems. IEEE Access, 7, 65821–65827. https://doi.org/10.1109/ACCESS.2019.2915575

Großmann, J., Pyttel, P., Bauhus, J., Lecigne, B., & Messier, C. (2020). The benefits of tree wounds: Microhabitat development in urban trees as affected by intensive tree maintenance. Urban Forestry & Urban Greening, 55, 126817. https://doi.org/10.1016/j.ufug.2020.126817

Hariyati, M., Bachtiar, I., & Sedijani, P. (2016). Induksi Kalus Tanaman Krisan (Chrysanthemum morifolium) Dengan Pemberian Benzil Amino Purin (BAP) Dan Dichlorofenoksi Acetil Acid (2,4 D). Jurnal Penelitian Pendidikan IPA, 2(1), 89–9. https://doi.org/10.29303/jppipa.v2i1.37

Hartman, B. J., & Eshenaur, B. (2025). Wounds and Wood Decay of Trees. Plant Pathology Fact Sheet. Retrieved from https://www.uaex.uada.edu/environment-nature/forestry/health/PPFS-OR-W-1.pdf

Hecht, U., Kohnle, U., Nill, M., Grüner, J., & Metzler, B. (2015). Bark wounds caused by felling are more susceptible to discoloration and decay than wounds caused by extraction in European beech. Annals of Forest Science, 72(6), 731–740. https://doi.org/10.1007/s13595-014-0432-y

Ikeuchi, M., Iwase, A., Rymen, B., Lambolez, A., Kojima, M., Takebayashi, Y., Heyman, J., Watanabe, S., Seo, M., De Veylder, L., Sakakibara, H., & Sugimoto, K. (2017). Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes. Plant Physiology, 175(3), 1158–1174. https://doi.org/10.1104/pp.17.01035

Ikeuchi, M., Ogawa, Y., Iwase, A., & Sugimoto, K. (2016). Plant regeneration: cellular origins and molecular mechanisms. Development, 143(9), 1442–1451. https://doi.org/10.1242/dev.134668

Ikeuchi, M., Sugimoto, K., & Iwase, A. (2013). Plant Callus: Mechanisms of Induction and Repression. The Plant Cell, 25(9), 3159–3173. https://doi.org/10.1105/tpc.113.116053

Iriani, Y. F., Prayantini, D. C., Nahampun, H. N., Mastuti, R., & Harijati, N. (2025). The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.). Jurnal Penelitian Pendidikan IPA, 11(8), 38–45. https://doi.org/10.29303/jppipa.v11i8.11884

Iwase, A., Kondo, Y., Laohavisit, A., Takebayashi, A., Ikeuchi, M., Matsuoka, K., Asahina, M., Mitsuda, N., Shirasu, K., Fukuda, H., & Sugimoto, K. (2021). WIND transcription factors orchestrate wound‐induced callus formation, vascular reconnection and defense response in Arabidopsis. New Phytologist, 232(2), 734–752. https://doi.org/10.1111/nph.17594

Kann, A. P., & Srivastava, M. (2025). A dynamic repertoire of wound closure strategies precedes whole-body regeneration. Retrieved from https://www.biorxiv.org/content/10.1101/2025.02.03.636261v2.full

Karunarathne, S. I., Spokevicius, A. V, Bossinger, G., & Golz, J. F. (2024). Trees need closure too: Wound-induced secondary vascular tissue regeneration. Plant Science, 339, 111950. https://doi.org/10.1016/j.plantsci.2023.111950

Kleczewski, N. M., & Bonello, P. (2009). Pruning and Care of Tree Wounds. The Ohio State University. Retrieved from https://ohioline.osu.edu/factsheet/HYG-3311-09

Lailani, Z. I., & Kuswandi, P. C. (2023). The effect of adding BAP on the induction of callus in porang plants in vitro. The Journal of Biological Studies, 9(1), 45–55. Retrieved from https://journal.student.uny.ac.id/kingdom/article/download/18481/17680

Lee, K., & Seo, P. J. (2018). Dynamic Epigenetic Changes during Plant Regeneration. Trends in Plant Science, 23(3), 235–247. https://doi.org/10.1016/j.tplants.2017.11.009

Lund, A., Wiström, B., Östberg, J., Andersson, A.-M., Gredvall, H., & Levinsson, A. (2026). Wound Occlusion Following Stem Injuries: A Comparative Analysis of Plastic Wrapped and Unwrapped Trees. Arboriculture & Urban Forestry, 014, jauf.2026.014. https://doi.org/10.48044/jauf.2026.014

Min, H., Kim, B., & Cha, B. (2016). Development of Wound-treatment Formulation Using Plant Growth Regulators for Wound Healing of Some Tree Species. The Korean Journal of Pesticide Science, 20(2), 83–92. https://doi.org/10.7585/kjps.2016.20.2.83

Neely, D. (1979). Tree Wounds and Wound Closure. Arboriculture & Urban Forestry, 5(6), 135–140. https://doi.org/10.48044/jauf.1979.033

Nooryazdan, N., Jourgholami, M., Picchio, R., Venanzi, R., & Lo Monaco, A. (2025). Long-Term Assessment of Wound Healing in Damaged Residual Trees Under Continuous Cover Forestry in the Hyrcanian Broad-Leaved Forests. Sustainability, 17(20), 9319. https://doi.org/10.3390/su17209319

O’Hara, K. L. (2007). Pruning wounds and occlusion: A long-standing conundrum in forestry. Journal of Forestry, 105(3), 131–138. https://doi.org/10.1093/jof/105.3.131

Ow, L. F., Ghosh, S., & Sim, E. K. (2013). Mechanical injury and occlusion: An urban, tropical perspective. Urban Forestry & Urban Greening, 12(2), 255–261. https://doi.org/10.1016/j.ufug.2013.02.004

Pouzoulet, J., Scudiero, E., Schiavon, M., & Rolshausen, P. E. (2017). Xylem Vessel Diameter Affects the Compartmentalization of the Vascular Pathogen Phaeomoniella chlamydospora in Grapevine. Frontiers in Plant Science, 8, 1442. https://doi.org/10.3389/fpls.2017.01442

Purcell, L. (2020). Tree wounds and healing. Purdue University, Forestry and Natural Resources. Retrieved from https://www.purdue.edu/fnr/extension/tree-wounds-and-healing/

Romeiro, D., Santos, C. M., Bucci, L. A., & Longui, E. L. (2021). As Anatomical Features Of The Xylem Could Influence Wound Healing Process In Trees? Revista Do Instituto Florestal, 33(2), 119–138. https://doi.org/10.24278/2178-5031.202133201

Safitri, S. K., Siregar, L. A. M., & Lubis, K. (2017). Callus induction of roselle (Hibiscus sabdariffa Linn.) on the explants type and different concentration of auxin. Jurnal Agroekoteknologi, 5(3), 593– 598. https://doi.org/10.32734/ja.v5i3.2222

Sari, M., & Isda, M. N. (2021). The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro. Jurnal Biologi UNAND, 9(1), 8–17. https://doi.org/10.25077/jbioua.9.1.8-17.2021

Sekar, A. A., Restiani, R., & Prasetyaningsih, A. (2023). Induksi Kalus Dari Eksplan Nodus Stelecocharpus burahol (Blume) Hook. f & Thomson sebagai upaya konservasi in vitro. BIOTIKA Jurnal Ilmiah Biologi, 21(1), 27–35. https://doi.org/10.24198/biotika.v21i1.42869

Self, B. (2020). Tree Wounds: Should They Be Repaired? (Vol. 3533). Mississippi University State. Retrieved from http://extension.msstate.edu/sites/default/files/publications/publications/P3533_web.pdf

Sinaulan, J. S., Lengkong, E. F., & Tulung, S. (2018). The response of the formation of the plant embryonic callus of krisan kulo (Chrysanthemum morifolium) to the substance of a growth regulator of cytokines. Cocos, 10(3), 1–9. Retrieved from https://ejournal.unsrat.ac.id/v3/index.php/cocos/article/view/22203

Sinclair, W. A., & Hudler, G. W. (1988). Tree Declines: Four Concepts of Causality. Arboriculture & Urban Forestry, 14(2), 29–35. https://doi.org/10.48044/jauf.1988.009

Slater, D. (2021a). The mechanical effects of bulges developed around bark-included branch junctions of hazel (Corylus avellana L.) and other trees. Trees, 35(2), 513–526. https://doi.org/10.1007/s00468-020-02053-z

Slater, D. (2021b). Inducing bark inclusions in branch junctions of aspen ( Populus tremula L.) by bracing them with horticultural wire. Arboricultural Journal, 43(4), 199–214. https://doi.org/10.1080/03071375.2021.1903211

Stobbe, H. (2002). Developmental Stages and Fine Structure of Surface Callus Formed after Debarking of Living Lime Trees (Tilia sp.). Annals of Botany, 89(6), 773–782. https://doi.org/10.1093/aob/mcf137

Suripto, F., Virgota, B., & Rahayu, R. N. (2024). Identifikasi Kerusakan Pohon Peneduh Di kampus Universitas Mataram Kumpulan Kertas Kerja Peserta Mata Kuliah Pengantar Ilmu Lingkungan. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mataram.

Suripto, S., & Aksari, S. Y. (2021). Evaluasi Ekologis Pohon Pelindung Kampus Universitas Mataram. Jurnal Pengabdian Magister Pendidikan IPA, 3(2), 247–257. https://doi.org/10.29303/jpmpi.v3i2.565

Suripto, S., Jupri, A., Farista, B., Virgota, A., & Ahyadi, H. (2021). Ecological valuation of city parks (Case study for Mataram City). Jurnal Biologi Tropis, 21(3), 1003–1012. https://doi.org/10.29303/jbt.v21i3.3026

Suripto, Supardiono, S., Rahayu, R. N., Dujana, L. M. A., & Genggelang, F. A. (2025). Analysis of Tree Fall Hazard Risk: A Case Study at the University of Mataram Campus. Jurnal Penelitian Pendidikan IPA, 11(10), 821–930. https://doi.org/10.29303/jppipa.v11i10.13045

Walangadi, F. R., Ahmad, J., Pagalla, D. B., Kandowangko, N. Y., & Febriyanti, F. (2025). Effect of BAP and NAA on Callus Emergence Time of Dumbaya Young Leaf Explants in Vitro. Jurnal Biologi Tropis, 25(2), 1903–1911. https://doi.org/10.29303/jbt.v25i2.9045

Author Biographies

Suripto, Faculty of Mathematics and Natural Science Universitas Mataram

Author Origin : Indonesia

Lalu Muhammad Aby Dujana, Faculty of Mathematics and Natural Science Universitas Mataram

Author Origin : Indonesia

Rachmawati Noviana Rahayu, Faculty of Mathematics and Natural Science Universitas Mataram

Author Origin : Indonesia

Supardiono, Faculty of Mathematics and Natural Science Universitas Mataram

Author Origin : Indonesia

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How to Cite

Suripto, Dujana, L. M. A., Rahayu, R. N., & Supardiono. (2026). The Influence of Stub Length and Texture of Pruning Wound Surface on the Wound Occlusion (Case Study on Tree Healt at the Mataram University Campus). Jurnal Penelitian Pendidikan IPA, 12(5), 118–127. https://doi.org/10.29303/jppipa.v12i5.14668