Growth Performance and Survival of Nile Tilapia (Oreochromis niloticus) with the Addition Combination of Tenebrio Molitor L. and Zingiber Officinale R. Flour
DOI:
10.29303/jppipa.v12i10.14955Published:
2026-10-14Downloads
Abstract
The combination of Tenebrio molitor flour and ginger Zingiber Officinale contains bioactive compounds that will synergistically enhance the growth performance and health of Nile tilapia Oreochromis Niloticus. The aim of the research is to analyse feed efficiency, weight gain and fish weight, specific growth rate of fish, and fish survival with the addition of a combination of tenebrio flour and ginger with an optimal composition using a randomised block design. including control, namely (100% commercial feed), Treatment 1 (70% commercial feed + 25% T. molitor flour + 0.5% ginger flour), treatment 2 (40% commercial feed + 50% T. molitor flour + 1.0% ginger flour), and treatment 3 (10% commercial feed + 75% T. molitor flour + 1.5% ginger flour). The parameters observed were feed efficiency, absolute length and weight gain, specific growth rate (SGR), and survival. The results showed that treatment with the composition (40% commercial feed + 50% T. molitor flour+ 10% ginger flour) gave the best results in feed efficiency (65.77%), length gain (5.00 cm), weight gain (8.75 grams), SGR (2.99%), and survival (95.83%). This research has resulted in a new innovation to enhance growth and maintain fish health, thereby ensuring food security and the successful development of the fish farming industry.
Keywords:
Growth performance Oreochromis niloticus Tenebrio molitor Zingiber officinaleReferences
Abozaid, H., Elnadi, A. S. M., Omer, H. A. A., El-Nomeary, Y. A. A., Aboelhassan, D. M., Awad, E., Abbas, W. T., Ebadah, I. M. A., & Moawad, S. S. (2024). Effect of Replacing Dietary Soybean Meal with Galleria mellonella Larvae Powder on Growth Performance of the Nile tilapia (Oreochromis niloticus). Egypt. J. Aquat. Biol Fish., 28(5), 123–148. https://doi.org/10.21608/ejabf.2024.377638 DOI: https://doi.org/10.21608/ejabf.2024.377638
Ahmadifar, E., Sheikhzadeh, N., Roshanaei, K., Dargahi, N., & Faggio, C. (2019). Can Dietary Ginger (Zingiber officinale) Alter Biochemical and Immunological Parameters and Gene Expression Related to Growth, Immunity and Antioxidant System in Zebrafish (Danio rerio)? Aquaculture, 507, 341–348. https://doi.org/10.1016/j.aquaculture.2019.04.049 DOI: https://doi.org/10.1016/j.aquaculture.2019.04.049
Ahmed, I., Jan, K., Fatma, S., & Dawood, M. A. O. (2021). Muscle Proximate Composition of Various Food Fish Species and Their Nutritional Significance: A Review. Animal Physiology and Animal Nutrition, 106(3), 690–719. https://doi.org/10.1111/jpn.13711 DOI: https://doi.org/10.1111/jpn.13711
Ashry, A. M., Habiba, M. M., El-Zayat, A. M., Badreldeen, A. H., Younis, N. A., Ahmed, H. A., El-Dakroury, M. F., Ali, M. A. M., & Dawood, M. A. O. (2024). Effects of Ginger (Zingiber officinale) on the Growth Performance, Digestive Enzyme Activity, Antioxidative Response, and Antibacterial Capacity of Striped Catfish (Pangasianodon hypophthalmus) Reared in Outdoor Conditions. Aquaculture Reports. https://doi.org/10.1016/j.aqrep.2023.101760 DOI: https://doi.org/10.1016/j.aqrep.2023.101760
Brum, A., Pereira, S. A., Owatari, M. S., Chagas, E. C., Chaves, F. C. M., Mourin˜o, J. L. P., & Martins, M. L. (2017). Effect of Dietary Essential Oils of Clove Basil and Ginger on Nile tilapia (Oreochromis niloticus) Following Challenge with Streptococcus agalactiae. Aquaculture, 468, 235–243. https://doi.org/10.1016/j.aquaculture.2016.10.020. DOI: https://doi.org/10.1016/j.aquaculture.2016.10.020
Eberle, S., Schaden, L. M., Tintner, J., Stauffer, C., & Schebeck, M. (2022). Effect of Temperature and Photoperiod on Development, Survival, and Growth Rate of Mealworms, Tenebrio Molitor. Insects, 13(4), 321. https://doi.org/10.3390/insects13040321 DOI: https://doi.org/10.3390/insects13040321
Eggink, K. M., Pedersen, P. B., Lund, I., & Dalsgaard, J. (2022). Chitin Digestibility and Intestinal Exochitinase Activity in Nile Tilapia and Rainbow Trout Fed Different Black Soldier Fly Larvae Meal Size Fractions. Aquaculture Research, 53(16), 5536-5546. https://doi.org/10.1111/are.16035 DOI: https://doi.org/10.1111/are.16035
El-Hack, M. E., El-Saadony, M. T., Nader, M. M., Salem, H. M., El-Tahan, A. M., Soliman, S. M., & Khafaga, A. F. (2022). Effect of Environmental Factors on Growth Performance of Nile Tilapia (Oreochromis niloticus). International Journal of Biometeorology, 66(11), 2183-2194. https://doi.org/10.1007/s00484-022-02347-6 DOI: https://doi.org/10.1007/s00484-022-02347-6
Francissca, N. E., & Muhsoni, F. F. (2021). Laju Pertumbuhan dan Kelangsungan Hidup Ikan Nila (Oreochromis niloticus) pada Salinitas yang Berbeda. Juvenil: Jurnal Ilmiah Kelautan dan Perikanan, 2(3). Retrieved from https://garuda.kemdiktisaintek.go.id/documents/detail/2289402
Gabriel, N. N. (2019). Review on the Progress in the Role of Herbal Extracts in Tilapia Culture. Cogent Food Agric., 5(1), 1619651. https://doi.org/10.1080/23311932.2019.1619651. DOI: https://doi.org/10.1080/23311932.2019.1619651
Hua, K. A. (2021). Meta-Analysis of the Effects of Replacing Fish Meals with Insect Meals on Growth Performance of Fish. Aquaculture, 530, 732-737. https://doi.org/10.1016/j.aquaculture.2020.735732 DOI: https://doi.org/10.1016/j.aquaculture.2020.735732
Indriati, D. A., Subandiyono, S., & Windarto, S. (2023). Effect of Monosodium Glutamate in Artificial Food on Feed Utilization Efficiency, Growth, and Survival of Fingerling Tilapia (Oreochromis niloticus). Aquacultura Indonesiana, 24(2), 76-87. http://doi.org/10.21534/ai.v24i2.25 DOI: https://doi.org/10.21534/ai.v24i2.25
Kanani, H. G., Nobahar, Z., Kakoolaki, S., & Jafarian, H. (2014). Effect of Ginger- and Garlic-Supplemented Diet on Growth Performance, Some Hematological Parameters and Immune Responses in Juvenile Huso huso. Fish Physiol. Biochem., 40(2), 481–490. https://doi.org/10.1007/s10695-013-9859-6 DOI: https://doi.org/10.1007/s10695-013-9859-6
Karlsen, O., Amlund, H., Berg, A., & Olsen, R. E. (2015). The Effect of Dietary Chitin on Growth and Nutrient Digestibility in Farmed Atlantic Cod, Atlantic Salmon and Atlantic Halibut. Aquaculture Research, 48(1), 123-133. https://doi.org/10.1111/are.12867 DOI: https://doi.org/10.1111/are.12867
Kim, H. S., Lee, K. W., Jung, H. S., Kim, J., Yun, A., Cho, S. H., & Kwon, M. (2018). Effects of Dietary Inclusion of Yacon, Ginger and Blueberry on Growth, Body Composition and Challenge Test of Juvenile Rockfish (Sebastes schlegeli) Against Edwardsiella tarda. Aquac. Nutr., 24(3), 1048–1055. https://doi.org/10.1111/anu.12643 DOI: https://doi.org/10.1111/anu.12643
Lai, W., Yang, S., Lin, X., Zhang, X., Huang, Y., Zhou, J., Fu, C., Li, R., & Zhang, Z. (2022). Zingiber officinale: A Systematic Review of Botany, Phytochemistry and Pharmacology of Gut Microbiota-Related Gastrointestinal Benefits. Am. J. Chin. Med., 50(04), 1007–1042. https://doi.org/10.1142/S0192415×22500410 DOI: https://doi.org/10.1142/S0192415X22500410
Lampová, B., Doskočil, I., Kulma, M., Kurečka, M., & Kouřimská, L. (2024). Culinary Treatments Impact the Digestibility and Protein Quality of Edible Insects: A Case Study with Tenebrio molitor and Gryllus assimilis. Front Nutr., 11. Retrieved from https://pubmed.ncbi.nlm.nih.gov/38883861/ DOI: https://doi.org/10.3389/fnut.2024.1399827
Lin, S-M., Zhou, X-M., Zhou, Y-L., Kuang, W-M., Chen, Y-J., Luo, L., & Dai, F-Y. (2020). Intestinal Morphology, Immunity and Microbiota Response to Dietary Fibers in Largemouth Bass, Micropterus salmoide. Fish Shellfish Immunology, 103, 135–142. https://doi.org/10.1016/j.fsi.2020.04.070 DOI: https://doi.org/10.1016/j.fsi.2020.04.070
Mariod, A. A. (2020). Chapter 20 - Nutrient Composition of Mealworm (Tenebrio molitor). In: Mariod A A. African Edible Insects as Alternative Source of Food, Oil, Protein and Bioactive Components. Gewerbestrasse: Springer Nature Swiss. http://dx.doi.org/10.1007/978-3-030-32952-5_20 DOI: https://doi.org/10.1007/978-3-030-32952-5_20
Mengistu, S. B., Mulder, H. A., Bastiaansen, J. W. M., Benzie, J. A. H., Khaw, H. L., & Trinh, T. Q. (2022). Komen H. Fluctuations in Growth Are Heritable and a Potential Indicator of Resilience in Nile tilapia (Oreochromis niloticus). Aquaculture, 560, 738481. https://doi.org/10.1016/j.aquaculture.2022.738481 DOI: https://doi.org/10.1016/j.aquaculture.2022.738481
Mohan, K., Ganesan, A. R., Muralisankar, T., Jayakumar, R., Sathish-Kumar, P., & Uthayakumar, V. (2020). Recent Insights into the Extraction, Characterization, and Bioactivities of Chitin and Chitosan from Insects. Trends Food Sci Technol., 105, 17–42. Retrieved from: https://pubmed.ncbi.nlm .nih.gov/32901176/ DOI: https://doi.org/10.1016/j.tifs.2020.08.016
Moruzzo, R., Riccioli, F., Diaz, S. E., Secci, C., Poli, G., & Mancini, S. (2021). Mealworm (Tenebrio molitor): Potential and Challenges to Promote Circular Economy. Animals: An Open Access Journal from MDPI, 11(9), 2568. https://doi.org/10.3390/ani11092568 DOI: https://doi.org/10.3390/ani11092568
Munguti, J. M., Nairuti, R., Iteba, J. O., Obiero, K. O., Kyule, D., Opiyo, M. A., Abwao, J., Kirimi, J. G., Outa, N., Muthoka, M., Githukia, C. M., & Ogello, E. O. (2022). Nile tilapia (Oreochromis niloticus Linnaeus, 1758) Culture in Kenya: Emerging Production Technologies and Socio-Economic Impacts on Local Livelihoods. Aquaculture, Fish and Fisheries, 2(4), 256. https://doi.org/10.1002/aff2.58 DOI: https://doi.org/10.1002/aff2.58
Ng, W. K., Liew, F. L., Ang, F. L., & Wong, K. W. (2022). Potential of Mealworm (Tenebrio molitor) as an Alternative Protein Source in Practical Diets for African Catfish, Clarias gariepinus. Aquaculture Research, 32, 273-280. https://doi.org/10.1046/j.1355-557x.2001.00024.x DOI: https://doi.org/10.1046/j.1355-557x.2001.00024.x
Noyens, I., Peer, M. V., Goossens, S., Heide, C. T., & Miert, S. V. (2024). The Nutritional Quality of Commercially Bred Yellow Meal-worm (Tenebrio molitor) Compared to European Union Nutrition Claims. Insects, 15(10). Retrieved from https://pubmed.ncbi.nlm.nih.gov/39452345/ DOI: https://doi.org/10.3390/insects15100769
Park, B. M., Lee, J., Jung, B. G., & Lee, B. J. (2022). Validation of the Anti-Inflammatory Effect of Tenebrio Molitor Larva Oil in a Colitis Mouse Model. Folia Biologica, 68(2), 50–58. Retrieved from https://pubmed.ncbi.nl m.nih.gov/36384262/ DOI: https://doi.org/10.14712/fb2022068020050
Pascon, G., Cardinaletti, G., Daniso, E., Bruni, L., Messina, M., Parisi, G., & Tulli, F. (2024). Effect of Dietary Chitin on Growth Performance, Nutrient Utilization, and Metabolic Response in Rainbow Trout (Oncorhynchus mykiss). Aquaculture Reports, 37, 102244. https://doi.org/10.1016/j.aqrep.2024.102244 DOI: https://doi.org/10.1016/j.aqrep.2024.102244
Pebiningrum, A., Kusnadi, J., & Rif'ah, H. I. (2017). Pengaruh Varietas Jahe (Zingiber officinale) dan Penambahan Madu Terhadap Aktivitas Antioksidan Minuman Fermentasi Kombucha Jahe. Journal of Food and Life Science, 1(2), 33-42. https://doi.org/10.21776/ub.jfls.2017.001.02.01
Ririhena, J. E., & Palinussa, E. M. (2021). Pertumbuhan dan Kelangsungan Hidup Ikan Nila (Oreochromis niloticus) di UPTD Budidaya Air Tawar. Jurnal Ilmiah Agribisnis dan Perikanan (Agrikan UMMU-Ternate), 14(2). DOI: https://doi.org/10.21107/juvenil.v2i3.11271
Sangsawang, A., Kovitvadhi, S., Pewhom, A., Kovitvadhi, U., Kovitvadhi, A., Wongoutong, C., Chatchaiphan, S., & Paankhao, N. (2024). Impacts of Substituting Fish Meal with Full-Fat or Defatted Black Soldier Fly (Hermetia illucens) Larvae on Growth, Quality, and Health of Nile tilapia (Oreochromis niloticus) Fingerlings. Aquac. Rep., 38, 102348. https://doi.org/10.1016/j.aqrep.2024.102348 DOI: https://doi.org/10.1016/j.aqrep.2024.102348
Sayed, A. F. M., & Fitzsimmons, K. (2022). From Africa to the world—The journey of Nile tilapia. Reviews in Aquaculture, 15, 6-21. https://doi.org/10.1111/raq.12738 DOI: https://doi.org/10.1111/raq.12738
Shafique, L., Abdel-Latif, H. M., Hassan, F. U., Alagawany, M., Naiel, M. A., Dawood, M. A., Yilmaz, S., & Liu, Q. (2021). The Feasibility of Using Yellow Mealworms (Tenebrio molitor): Towards a Sustainable Aquafeed Industry. Animals, 11(3), 811. https://doi.org/10.3390/ani11030811 DOI: https://doi.org/10.3390/ani11030811
Stastnik, O., Novotny, J., Roztocilova, A., Kouril, P., Kumbar, V., Cernik, J., Kalhotka, L., Pavlata, L., Lacina, L., & Mrkvicova, E. (2021). Safety of Mealworm Meal in Layer Diets and Their Influence on Gut Morphology. Animals, 11(5), 1439. Retrieved from https://www.mdpi.com/2076-2615/11/5/1439/html DOI: https://doi.org/10.3390/ani11051439
Zhang, J., Dong, Y., Song, K., Wang, L., Li, X., Tan, B., Lu, K., & Zhang, C. (2022). Effects of the Replacement of Dietary Fish Meal with Defatted Yellow Mealworm (Tenebrio molitor) on Juvenile Large Yellow Croakers (Larimichthys crocea) Growth and Gut Health. Animals, 12(19), 2659. https://doi.org/10.3390/ani12192659 DOI: https://doi.org/10.3390/ani12192659
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