Correlation Analysis between Understanding Biochemistry Concepts and Chemistry Education Students' Problem-Solving Skills through Validated HOTS Instruments
DOI:
10.29303/jppipa.v12i6.14982Published:
2026-06-25Downloads
Abstract
Understanding biochemistry concepts and problem-solving skills are essential competencies for chemistry education students in facing the demands of 21st-century science learning. However, empirical evidence regarding the relationship between these competencies measured through Higher Order Thinking Skills (HOTS)-based instruments remains limited. This study aimed to analyze the correlation between students’ understanding of biochemistry concepts and their problem-solving skills using validated HOTS instruments. A quantitative correlational design was employed involving 60 Chemistry Education students who had completed the Biochemistry course. Data were collected using a HOTS-based biochemistry concept understanding test and a HOTS-based problem-solving skills test. The instruments were validated by experts and tested for reliability. Data were analyzed using descriptive statistics and Pearson correlation analysis after fulfilling normality and linearity assumptions. The results showed that the mean score of biochemistry concept understanding was 72.35 (SD = 8.42), while the mean score of problem-solving skills was 68.10 (SD = 9.15). The normality and linearity tests indicated that the data met the requirements for parametric analysis. Pearson correlation analysis revealed a positive and significant correlation between biochemistry concept understanding and problem-solving skills (r = 0.63, p < 0.05). Furthermore, the coefficient of determination (r² = 0.40) indicated that understanding biochemistry concepts contributed 40% to the variance in students’ problem-solving skills. These findings demonstrate that stronger conceptual understanding is associated with better problem-solving performance. In conclusion, understanding biochemistry concepts significantly contributes to the development of students’ problem-solving skills. Therefore, integrating HOTS-oriented learning and assessment into biochemistry instruction is recommended to enhance conceptual mastery and higher-order thinking skills among prospective chemistry teachers.
Keywords:
Biochemistry education Chemistry teacher education Conceptual understanding Higher-order thinking skills Problem-solving skillsReferences
Abdullah, A. H., Abidin, N. L. Z., & Ali, M. (2015). Analysis of students’ errors in solving Higher Order Thinking Skills (HOTS) problems for the topic of fraction. Asian Social Science, 11(21). https://doi.org/10.5539/ass.v11n21p133 DOI: https://doi.org/10.5539/ass.v11n21p133
Abdullah, A. H., Mokhtar, M., Halim, N. D. A., Ali, D. F., Tahir, L. M., & Kohar, U. H. A. (2017). Mathematics teachers’ level of knowledge and practice on the implementation of higher-order thinking skills (HOTS). Eurasia Journal of Mathematics, Science and Technology Education, 13(1). https://doi.org/10.12973/eurasia.2017.00601a DOI: https://doi.org/10.12973/eurasia.2017.00601a
Amanda, F. F. (2022). Pengembangan pembelajaran problem based learning terintegrasi pendekatan complexity science berbantuan mindmap untuk meningkatkan kemampuan berpikir kritis, kreativitas dan pemecahan masalah mahasiswa biologi. Universitas Negeri Malang.
Anwar, Y. A. S., Junaidi, E., & Al Idrus, S. W. (2013). Analisis kesulitan belajar Biokimia mahasiswa dalam upaya pengembangan perangkat pembelajaran berbasis daur belajar Johnston. PAEDAGORIA Jurnal Kajian Penelitian dan Pengembangan Kependidikan, 8(2), 71-74. https://doi.org/10.31764/paedagoria.v5i1.47
Arofiq, A. (2024). Pengembangan Lembar Kerja Siswa Berbasis Masalah Untuk Peningkatkan Kemampuan Metakognitif Dan Kompetensi Ekologis Siswa. Edubiologica: Jurnal Penelitian Ilmu Dan Pendidikan Biologi, 8(2). https://doi.org/10.25134/edubiologica.v8i2.10225 DOI: https://doi.org/10.25134/edubiologica.v8i2.10225
Aryani, I. (2024). Analisis Kesalahan Siswa Dalam Menyelesaikan Soal Matematika Melalui Higher Order Thinking Skill (HOTS). Jurnal Serambi Ilmu, 20(2). https://doi.org/10.32672/jsi.v20i2.1088 DOI: https://doi.org/10.32672/jsi.v20i2.1088
Astuti, R., Setianingsih, G. M., & Rahayu, S. (2021). Efektifitas Praktikum biokimia berbasis laboratory virtual di masa pandemi COVID-19: Studi komparasi praktikum luring dan daring untuk meningkatkan pemahaman konsep materi protein pada mahasiswa. Jurnal inovasi pendidikan dan sains, 2(1), 13-18. Retrieved from https://shorturl.at/FlaCP DOI: https://doi.org/10.51673/jips.v2i1.498
Asyifa, S. (2025). Strategi Bioproduksi Berkelanjutan Dari Limbah Pertanian: Integrasi Konsep Circular Economy Untuk Minimasi Emisi Dan Nilai Tambah Tinggi. Jurnal Teknologi Kimia Unimal, 14(1). https://doi.org/10.29103/jtku.v14i1.21667 DOI: https://doi.org/10.29103/jtku.v14i1.21667
Bretz, S. L. (2019). Evidence for the importance of learning progressions in biochemistry education. Biochemistry and Molecular Biology Education, 47(2), 109–112. https://doi.org/10.1021/acs.jchemed.8b00874 DOI: https://doi.org/10.1021/acs.jchemed.8b00874
Fadillah, M. A., Tanjung, Y. I., Usmeldi, U., Lufri, L., Festiyed, F., & Mawardi, M. (2025). Review Metodologi Penelitian Dalam Pengembangan Model Inquiry Untuk Pendidikan. Jurnal Muara Pendidikan, 10(1). https://doi.org/10.52060/mp.v10i1.2755 DOI: https://doi.org/10.52060/mp.v10i1.2755
Fibriani, S., Agustien, I. D., Sawitri, W. D., & Sugiharto, B. (2019). Transformasi Genetik Dan Ekspresi Mutan Sucrose Phosphate Synthase Pada Tanaman Tomat. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 6(1). https://doi.org/10.29122/jbbi.v6i1.3341 DOI: https://doi.org/10.29122/jbbi.v6i1.3341
Himmah, W. I. (2019). Analisis Soal Penilaian Akhir Semester Mata Pelajaran Matematika Berdasarkan Level Berpikir. Journal of Medives : Journal of Mathematics Education IKIP Veteran Semarang, 3(1). https://doi.org/10.31331/medivesveteran.v3i1.698 DOI: https://doi.org/10.31331/medivesveteran.v3i1.698
Izzati, N., & Febrian, F. (2021). Kemampuan Mahasiswa Calon Guru Dalam Mengembangkan Instrumen Tes Hasil Belajar Kategori Higher Order Thinking Skill. Jurnal Gantang, 6(1). https://doi.org/10.31629/jg.v6i1.2672 DOI: https://doi.org/10.31629/jg.v6i1.2672
Jelmila, S. N., Mutiya Rahmi, R., Suharni, Helmizar, R., & Liana, N. (2026). Status Vitamin D dan Hubungannya dengan Kadar Kolesterol Total Mahasiswa Fakultas Kedokteran Universitas Baiturrahmah. Scientific Journal, 5(1). https://doi.org/10.56260/sciena.v5i1.321 DOI: https://doi.org/10.56260/sciena.v5i1.321
Jim, E. L. (2014). Metabolisme Lipoprotein. Jurnal Biomedik (JBM), 5(3). https://doi.org/10.35790/jbm.5.3.2013.4335 DOI: https://doi.org/10.35790/jbm.5.3.2013.4335
Karitas, C., & Suwartono, C. (2023). e-LIT: Pengembangan Instrumen Penilaian Literasi Digital bagi Generasi Digital-Native. Jurnal Penelitian Dan Pengukuran Psikologi: JPPP, 12(2). https://doi.org/10.21009/jppp.122.08 DOI: https://doi.org/10.21009/JPPP.122.08
Khalidah, A. R. (2022). Struktur Biomolekul dan Mekanisme Aksi Vaksin Sars-Cov-2 Efektif dalam Melawan Covid-19. Jurnal Ilmu Medis Indonesia, 2(1). https://doi.org/10.35912/jimi.v2i1.1006 DOI: https://doi.org/10.35912/jimi.v2i1.1006
Muchtar, Z., Gusfa, M., Dibyanti, R. E., Sutiani, A., & Sinaga, M. (2024). Pengembangan Instrumen Evaluasi untuk Mengukur Keterampilan Berpikir Tingkat Tinggi pada Materi Laju Reaksi. JIIP - Jurnal Ilmiah Ilmu Pendidikan, 7(8). https://doi.org/10.54371/jiip.v7i8.4901 DOI: https://doi.org/10.54371/jiip.v7i8.4901
Muhammad, N., M Nur, Y. I., & Widarta, F. O. (2022). Implementasi Flipped Classroom Terhadap Pemahaman Konsep Dan Motivasi Belajar Mahasiswa Pada Materi Metabolisme Lipid. Jurnal Jeumpa, 9(1). https://doi.org/10.33059/jj.v9i1.5414 DOI: https://doi.org/10.33059/jj.v9i1.5414
Novitasari, R. (2017). Proses respirasi seluler pada tumbuhan. In Prosiding Seminar Nasional Pendidikan Biologi (Vol. 1, No. 1, pp. 89-96). Retrieved from https://www.studocu.id/id/document/universitas-papua/biologi-kehutanan/proses-respirasi-seluler-pada-tumbuhan/47397802
Nurjayadi, M., Saputra, I., Putri, G. I., Declan, J. L., Krisdawati, I., Juliansyah, D. A., & Hairishah, N. (2023). Diseminasi Pembelajaran Struktur Dan Fungsi Biomolekul Protein Bagi Guru Wilayah Mgmp-1 Jakarta Timur. Sarwahita, 20(01). https://doi.org/10.21009/sarwahita.201.3
Nurjayadi, M., Saputra, I., Putri, G. I., Declan, J. L., Krisdawati, I., Juliansyah, D. A., & Hairishah, N. (2023). Struktur Dan Fungsi Biomolekul Protein. Sarwahita, 20(01). https://doi.org/10.21009/sarwahita.201.3 DOI: https://doi.org/10.21009/sarwahita.201.3
Pangestuti, A. F. N., Ana, S. U., Syahra, E. F. A., & Sari, S. N. (2024). Analisis Distribusi Soal Higher Order Thinking Skills (HOTS) dan Lower Order Thinking Skills (LOTS) dalam Buku Ajar Bahasa Indonesia. Indonesian Journal of Action Research, 3(1), 25-31. https://doi.org/10.14421/ijar.2024.31-03 DOI: https://doi.org/10.14421/ijar.2024.31-03
Prihatini, I., & Dewi, R. K. (2021). Kandungan Enzim Papain pada Pepaya (Carica papaya L) Terhadap Metabolisme Tubuh. Jurnal Tadris IPA Indonesia, 1(3). https://doi.org/10.21154/jtii.v1i3.312 DOI: https://doi.org/10.21154/jtii.v1i3.312
Qudsiah, J. R., Fahrurrozi, & Hayati, N. (2023). Pengembangan instrumen tes kategori higher order thinking skills (HOTS) pada mata pelajaran matematika kelas XI SMA. NOTASI: Jurnal Pendidikan Matematika, 1(1). https://doi.org/10.70115/notasi.v1i1.48 DOI: https://doi.org/10.70115/notasi.v1i1.48
Rahmawati, N. D., & Faizah, S. (2023). Analisis Instrumen Penilaian Berbasis Ethnicmath Hots Pada Matakuliah Matematika Dasar. Prosiding Seminar Nasional Sains, Teknologi, Ekonomi, Pendidikan Dan Keagamaan, 7(December). Retrieved from https://shorturl.at/qa8or
Sari, A. F., Eka Putri, L. S., Hariwibowo, D. R., Riliansyah, A., Sugoro, I., Mujiyanto, A., & Hamada, F. R. (2023). Isolasi dan Identifikasi Mikroorganisme Dari Produk Ekoenzim WOP FST 1310. Jurnal Penelitian Sains, 25(3). https://doi.org/10.56064/jps.v25i3.876 DOI: https://doi.org/10.56064/jps.v25i3.876
Setiadi, R. (2013). Pengantar Biokimia. In Biokimia. Universitas Terbuka.
Sinta, U. A., Roebyanto, G., & Nuraini, N. L. S. (2022). Analisis Kesulitan Guru dalam Menyusun Soal Evaluasi Berbasis Hots Pada Pembelajaran Matematika di SDN Torongrejo 2. Jurnal Pembelajaran, Bimbingan, Dan Pengelolaan Pendidikan, 2(1). https://doi.org/10.17977/um065v2i12022p45-53 DOI: https://doi.org/10.17977/um065v2i12022p45-53
Suhartono, I. M. T. (2017). Protein-serial biokimia mudah dan menggugah. Gramedia Widiasarana Indonesia.
Sukowati, I., & Ekawati, R. (2024). Pengembangan Instrumen Asesmen Literasi Muatan IPAS Berbasis HOTS Fase B Kelas 4 Sekolah Dasar. JIIP - Jurnal Ilmiah Ilmu Pendidikan, 7(2). https://doi.org/10.54371/jiip.v7i2.3843 DOI: https://doi.org/10.54371/jiip.v7i2.3843
Sulistiany, H., & Darmawan, H. (2020). Pengembangan Modul Praktikum Biokimia Untuk Meningkatkan Keterampilan Proses Sains Mahasiswa Pendidikan Biologi. Bioma : Jurnal Ilmiah Biologi, 9(2). https://doi.org/10.26877/bioma.v9i2.7057 DOI: https://doi.org/10.26877/bioma.v9i2.7057
Sutedjo, A. (2013). Implementasi dan Evaluasi Pembelajaran E-Learning Pada Mata Kuliah Geografi Transportasi dan Komunikasi Mahasiswa yang Memprogram di Semester Gasal Tahun Akademik 2012/2013. Jurnal Geografi, 11(21). Retrieved from https://garuda.kemdiktisaintek.go.id/documents/detail/140568
Syafryadin, Harahap, A., Haryani, & Astrid, A. (2021). Boosting classroom interaction based on higher order thinking skills (Hots) in english learning for beginners. International Journal of Language Education, 5(1). https://doi.org/10.26858/IJOLE.V5I1.15211 DOI: https://doi.org/10.26858/ijole.v5i1.15211
Venkatraman, S., Benli, F., Wei, Y., & Wahr, F. (2022). Smart Classroom Teaching Strategy to Enhance Higher Order Thinking Skills (HOTS)—An Agile Approach for Education 4.0. Future Internet, 14(9). https://doi.org/10.3390/fi14090255 DOI: https://doi.org/10.3390/fi14090255
Wardhana, D. E. C., & Rofi'i, A. (2022). Students' perspective and problems in implementing higher order thinking skill (HOTS) in speaking for presentation class. Journal of Language and Linguistic Studies, 18(1), 477-487. Retrieved from https://eric.ed.gov/?id=EJ1325854
Wijayanti, T., & Lestari, P. B. (2018). Modifikasi Materi Praktikum (Karbohidrat-Enzim) Untuk Meningkatkan Pemahaman Konsep Biokimia Secara Komprehensif dan Efisien. Paradigma: Jurnal Filsafat, Sains, Teknologi, Dan Sosial Budaya, 23(2). https://doi.org/10.33503/paradigma.v23i2.406 DOI: https://doi.org/10.33503/paradigma.v23i2.406
Wijayanti, T., & Lestari, P. B. (2018). Modifkasi Materi Praktikum (Karbohidrat-Enzim) Untuk Meningkatkan Pemahaman Konsep Biokimia Secara Komprehensif dan Efsien. Paradigma: Jurnal Filsafat, Sains, Teknologi, Dan Sosial Budaya, 22(2). https://doi.org/10.33503/paradigma.v22i2.491 DOI: https://doi.org/10.33503/paradigma.v22i2.491
Yanuarti, E., Karolina, A., & Sari, D. P. (2020). Peran Pemerintah Dalam Mencegah Tindakan Radikalisme Melalui Pendidikan Multikultural. POTENSIA: Jurnal Kependidikan Islam, 5(2). https://doi.org/10.24014/potensia.v5i2.7499 DOI: https://doi.org/10.24014/potensia.v5i2.7499
Zulfayani, Z., Ariawan, R., Nufus, H., Nurdin, E., & Fitriani, D. (2024). Pengembangan Instrumen Kemampuan Literasi Numerasi Berbasis HOTS. Jurnal Cendekia : Jurnal Pendidikan Matematika, 8(1). https://doi.org/10.31004/cendekia.v8i1.2796 DOI: https://doi.org/10.31004/cendekia.v8i1.2796
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