Nanogel Formulation of Butterfly Pea Flower Extract (Clitoria ternatea L.) Against Propionibacterium acnes Bacteria Inhibitory Test
DOI:
10.29303/jppipa.v12i6.15211Published:
2026-06-25Downloads
Abstract
The chemical components of butterfly pea flowers (Clitoria ternatea L.) have a strong bacterial inhibition zone. The aim of this research is to make nanogels using the active ingredient ethanol extract of butterfly pea flowers and demonstrate the antibacterial activity of nanogels against Propionibacterium acnes bacteria. Because nanogels are more stable, translucent, and have small particle sizes making them efficient in releasing active chemicals and increasing bioavailability, nanogels are used in tropical medicine. Maceration is the extraction technique used. Nanogel of butterfly pea flower ethanol extract (BPFEE) was added to the nanogel formulation at concentrations of F1 (5%), F2 (10%), and F3 (15%). Transmission testing is part of the ideal formula; Other tests for characterization include phytochemical, organoleptic, particle size, pH, adhesion, spreadability and viscosity tests. Next, antibacterial activity was tested against Propionibacterium acnes using the well method. The research results showed that the % transmittance was 84-88%, the organoleptic results were in the form of a gel, color and distinctive aroma, particle size 114.9 – 283.9 mm, pH 5.6 – 6, adhesive power 1.36 – 2.1 seconds, spreadability 4.8-5.6 cm, viscosity 4071 – 3043.75 mPa.S. Telang flower ethanol extract nanogel can inhibit the growth of P. acnes bacteria, namely 8.2-11.7 mm.
Keywords:
Antibacterial activity Butterfly pea flower NanogelReferences
Achrifa, S. I., & Suyatno. (2024). Phytochemical Screening and Nanoherbs Synthesis of Ethanol Extract of the Butterfly Pea Flower (Clitoria ternatea L.) with its Characterization. Jurnal Pijar MIPA, 19(1). https://doi.org/10.29303/jpm.v19i1.6125 DOI: https://doi.org/10.29303/jpm.v19i1.6125
Amalia, A., & Sari, I. (2017). Antibacterial Activity of Ethyl Acetate Extract of Sembung Leaves (Blumea balsamifera (L.) DC.) Against the Growth of Methicillin-Resistant Staphylococcus aureus (MRSA) Bacteria. Prosiding Seminar Nasional BIOTIK (Biologi, Teknik dan Kependidikan, 5(1). https://doi.org/10.22373/pbio.v5i1.2160
Amalia, T. R., Maulidya, V., & Sastyarina, Y. (2024). Characterization and Effect of Chitosan Composition on the Size Stability of Dayak Onion Extract Nanoparticles (Eleutherine americana Merr.) Using the Ionic Gelation Method. Jurnal Mandala Pharmacon Indonesia, 10(1), 68–73. https://doi.org/10.35311/jmpi.v10i1.487 DOI: https://doi.org/10.35311/jmpi.v10i1.487
Ariyani, L. W., & Wulandari. (2020). Formulation of Olive Oil Nanogel Preparation as Antiacne. Jurnal Ilmiah Cendekia Eksakta, 5(2), 92–100. https://doi.org/10.3194/ce.v5i2.3737
Ayu Martini, N. K., Ayu Ekawati, N. G., & Timur Ina, P. (2020). The Effect of Temperature and Drying Time on the Characteristics of Butterfly Pea Tea (Clitoria ternatea L.). Jurnal Ilmu dan Teknologi Pangan (ITEPA), 9(3), 327. https://doi.org/10.24843/itepa.2020.v09.i03.p09 DOI: https://doi.org/10.24843/itepa.2020.v09.i03.p09
Bia, C., & Minarsih, T. (2025). The Effect of Nanotechnology in the Formulation of Butterfly Pea Flower (Clitoria Ternatea) Extract Cream on the Antibacterial Activity of Acne-Causing Propionibacterium acnes. Journal of Science and Technology Research for Pharmacy, 5(1). Retrieved from https://journal.unnes.ac.id/journals/JSTRP DOI: https://doi.org/10.15294/jstrp.v5i1.27310
Cahyaningsih, E., Yuda, P. E. S. K., & Santoso, P. (2019). Phytochemical Screening and Antioxidant Activity Test of Ethanol Extract of Butterfly Pea Flower (Clitoria Ternatea L.) Using UV-Vis Spectrophotometry Method. Jurnal Ilmiah Medicamento, 5(1). https://doi.org/10.36733/medicamento.v5i1.851 DOI: https://doi.org/10.36733/medicamento.v5i1.851
Chandra, D., Sinaga, T. R., Manik, T. R. A., & Aruan, T. K. (2021). Nanocream Formulation of Katuk Leaf Extract (Sauropus androgynus L.Merr.) as a Skin Moisturizer. TEKESNOS, 3(1), 374–385. Retrieved from https://e-journal.sari-mutiara.ac.id/index.php/tekesnos/article/view/4141/2781
Crendhuty, F. D., Sriwidodo, S., & Wardhana, Y. W. (2020). Chitosan Biopolymer-Based Drug Delivery System as a Film Forming System. Majalah Farmasetika, 6(1). https://doi.org/10.24198/mfarmasetika.v6i1.27457 DOI: https://doi.org/10.24198/mfarmasetika.v6i1.27457
Dewanti, A. A., Andriani, D., & Utami, N. (2023). Preparation Nanoparticles of Ethanol Extract of Telang Flower (Clitoria ternatea L.) with Variation Concentration of Chitosan and Tripolyphosphate as Antioxidant Candidates. Indonesian Journal of Pharmacy and Natural Product, 6(01), 39-44. https://doi.org/10.35473/ijpnp.v6i01.2128 DOI: https://doi.org/10.35473/ijpnp.v6i01.2128
Dewi, N. P. (2020). Quantitative Test of Standard Metabolites of Ethanol Extract of Awar-Awar Leaves (Ficus Septica Burm. F) Using Chromatography Method. Acta Holistica Pharmaciana, 2(1), 16-24.
Emelda., Safitri, E. A., & Fatmawati, A. (2021). Inhibitory Activity of Ethanolic Extract of Ulva Lactuca Against Staphylococcus Aureus Bacteria. Pharmaceutical Journal of Indonesia, 7(1), 43–48. https://doi.org/10.21776/ub.pji.2021.007.01.7 DOI: https://doi.org/10.21776/ub.pji.2021.007.01.7
Farhan, M. (2023). Formulation and Physical Quality Testing of Butterfly Pea Flower Extract Gel Preparation (Clitoria ternatea L.) as a Hand Antiseptic. Jurnal Penelitian Farmasi & Herbal, 5(2), 1-12. https://doi.org/10.36656/jpfh.v5i2.1000 DOI: https://doi.org/10.36656/jpfh.v5i2.1000
Habibie, D. R., & Aldo, D. (2019). Expert System for Identification of Acne Types Using Certainty Factor Method. Journal of Information Technology and Computer Science, 4(3), 79. https://doi.org/10.31328/jointecs.v4i3.1055 DOI: https://doi.org/10.31328/jointecs.v4i3.1055
Hakim, R. I., Wilson, W., & Darmawati, S. (2019). Uji Aktivitas Antibakteri Ekstrak Ethanol Daun Kayu Putih (Melaleuca leucadendron L.) Terhadap Pertumbuhan Methicillin Resistant Staphylococcus aureus (MRSA). Prosiding Seminar Nasional Mahasiswa Unimus 2, 109-115. Retrieved from https://prosiding.unimus.ac.id/index.php/mahasiswa/article/view/448
Hamka, Z., Noena, R. A. N., & Azmin, R. A. P. (2022). Pengaruh Metode Maserasi Bertingkat Terhadap Nilai Rendemen dan Profil Kramotografi Lapis Tipis (KLT) Ekstrak Daun Kemangi (Ocimum basilicum L.). Jurnal Kesehatan Yamasi Makassar, 6(1), 154–162. Retrieved from https://jurnal.yamasi.ac.id/index.php/Jurkes/article/view/213
Jain, R., Singh, R., Badhwar, R., Gupta, T., & Popli, H. (2024). Development and Optimization of Clitoria Teratea Synthesized Silver Nanoparticles and its Application to Nanogel Systems for Wound Healing. Drug Development and Industrial Pharmacy, 50(3), 181-191. https://doi.org/10.1080/03639045.2024.2308043 DOI: https://doi.org/10.1080/03639045.2024.2308043
Jawa La, E. O., & Sawiji, R. T. (2024). Formulation and Activity Test of Anti-Acne Gel Preparation from Ethanol Extract of Butterfly Pea Flower (Clitoria ternatea L.). PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 21(1), 20–25. https://doi.org/10.30595/pharmacy.v21i1.15680 DOI: https://doi.org/10.30595/pharmacy.v21i1.15680
Juliantoni, Y., Hajrin, W., & Subaidah, W. A. (2020). Nanoparticle Formula Optimization of Juwet Seeds Extract (Syzygium cumini) using Simplex Lattice Design Method. Jurnal Biologi Tropis, 20(3), 416–422. https://doi.org/10.29303/jbt.v20i3.2124 DOI: https://doi.org/10.29303/jbt.v20i3.2124
Kementerian Kesehatan RI. (2021). Guidelines for the Use of Antibiotics. Departemen Kesehatan RI
Kindangen, O. C., Yamlean, P. V. Y., & Wewengkang, D. S. (2018). Formulasi Gel Antijerawat Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) dan Uji Aktivitasnya Terhadap Bakteri Staphylococcus aureus Secara In Vitro. PHARMACON, 7(3), 238–293. https://doi.org/10.35799/pha.7.2018.20505
Kurniasari, D., & Atun, S. (2017). Preparation and Characterization of Nanoparticles of Ethanol Extract of Temu Kunci (Boesenbergia Pandurata) in Various Chitosan Composition Variations. Jurnal Sains Dasar, 6(1), 31. https://doi.org/10.21831/jsd.v6i1.13610 DOI: https://doi.org/10.21831/jsd.v6i1.13610
Larasati, D., & Putri, F. M. S. (2023). Phytochemical Screening and Determination of Flavonoid Content of Ethanol Extract of Banana Peel Waste (Musa acuminata Colla). Mandala Pharmacon Indonesia Journal, 9(1), 125-131. https://doi.org/10.35311/jmpi.v9i1.330 DOI: https://doi.org/10.35311/jmpi.v9i1.330
Li, C., Tang, W., Chen, S., He, J., Li, X., Zhu, X., Li, H., & Peng, Y. (2022). Phytochemical Properties and In Vitro Biological Activities of Phenolic Compounds from Flower of Clitoria ternatea L. Molecules, 27(19). https://doi.org/10.3390/molecules27196336 DOI: https://doi.org/10.3390/molecules27196336
Magani, A. K., Tallei, T. E., & Kolondam, B. J. (2020). Antibacterial Test of Chitosan Nanoparticles on the Growth of Staphylococcus aureus and Escherichia coli Bacteria. Jurnal Bios Logos, 10(1), 7. https://doi.org/10.35799/jbl.10.1.2020.27978 DOI: https://doi.org/10.35799/jbl.10.1.2020.27652
Maqbool, H., Visnuvinayagam, S., Zynudheen, A. A., Safeena, M. P., & Kumar, S. (2020). Antibacterial Activity of Beetroot Peel and Whole Radish Extract by Modified Well Diffusion Assay. Int. J. Curr. Microbiol. App. Sci, 9(1), 1222-1231. https://doi.org/10.20546/ijcmas.2020.901.135 DOI: https://doi.org/10.20546/ijcmas.2020.901.135
Ma'ruf, M., Bachri, M. S., & Nurani, L. H. (2023). Phytochemical Screening Analysis and Determination of Total Flavonoids and Total Phenolics Content of Ethanol Extract of Sungkai Leaf (Penorema canescens Jack) from Samarinda City. Mandala Pharmacon Indonesia Journal, 9(2), 262-272. https://doi.org/10.35311/jmpi.v9i2.360 DOI: https://doi.org/10.35311/jmpi.v9i2.360
Meilina, N. E., & Hasanah, A. N. (2018). Article Review: Antibacterial Activity of Mangosteen (Garnicia Mangostana L.) Peel Extract Against Acne-Causing Bacteria. Farmaka Suplemen, 16(2), 322–328. https://doi.org/10.24198/jf.v16i2.17550
Nabila, F. S., Radhityaningtyas, D., Yurisna, V. C., Listyaningrum, F., & Aini, N. (2022). Potential of Butterfly Pea Flowers (Clitoria ternatea L.) as Antibacterials in Food Products. Jitipari, 7(1). Retrieved from http://ejurnal.unisri.ac.id/index.php/jtpr/index
Pertiwi, F. D., Rezaldi, F., & Puspitasari, R. (2022). Antibacterial Activity Test of Ethanol Extract of Butterfly Pea Flower (Clitoria ternatea L.) Against Staphylococcus epidermidis Bacteria. Biosaintropis (Bioscience-Tropic), 7(2), 57–68. https://doi.org/10.33474/e-jbst.v7i2.471 DOI: https://doi.org/10.33474/e-jbst.v7i2.471
Pradana, T. B., Nugroho, A. E., & Martien, R. (2023). Systematic Review: Nanoparticles from Ingredients in Indonesian Traditional Medicines. Majalah Farmaseutika, 19. https://doi.org/10.22146/farmaseutik.v19i4.91236 DOI: https://doi.org/10.22146/farmaseutik.v19i4.91236
Pratiwi, R. H. (2017). Pathogenic Bacterial Defense Mechanisms Against Antibiotics. Jurnal Pro Life, 4. https://doi.org/10.33541/pro-life.v4i3.479
Purwandari, V., Sianipar, A. Y., Cinthya, Y., Silalahi, E., & Nasution, D. J. (2020). Antibacterial Effectiveness Test of Nano Gel Active Ingredient Cinnamon Extract (Cinnamomum burmannii) Against Staphylococcus Aureus. Farmanesia, 7(2), 92–99.2 https://doi.org/10.51544/jf.v7i2.2776 DOI: https://doi.org/10.51544/jf.v7i2.2776
Putri, B. R., Ulfa, A. M., & Marcellia, S. (2022). Formulation and Activity Test of Anti-Acne Cream Preparations of Ethanol Extract of Butterfly Pea Flower (Clitoria ternatea) Against 10 Propionibacterium acnes. Jurnal Ilmu Kedokteran dan Kesehatan, 8(4). https://doi.org/10.33024/jikk.v8i4.5262 DOI: https://doi.org/10.33024/jikk.v8i4.5262
Tandi, J., Mariani, N. M. I., & Setiawati, N. P. (2020). The Potential of Ethanol Extract of African Leaves (Gymnanthemum Amygdalinum (Delile) sch. bip, ex walp) on Reducing Blood Glucose Levels and Pancreatic Histopathology of Male White Rats (Rattus Norvegicus) Induced by Streptocotocin and High-Fat Feed. Majalah Farmasetika., 4(1), 66–77. https://doi.org/10.24198/mfarmasetika.v4i0.25861 DOI: https://doi.org/10.24198/mfarmasetika.v4i0.25861
Vinay, C. H. (2019). Development and Characterization of Nanoparticles Based Gels from Extraction of Different Fruit Peels. Rajiv Gandhi University of Health Sciences.
Wardani, H. N. (2020). The Potential of Soursop Leaf Extract in Treating Acne-Prone Facial Skin. Jurnal Penelitian Perawat Profesional, 2(4), 563–570. Retrieved from http://jurnal.globalhealthsciencegroup.com/index.php/JPPP DOI: https://doi.org/10.37287/jppp.v2i4.218
Windy, Y. M., Dilla, K. N., Claudia, J., Noval, N., & Hakim, A. R. (2022). Characterization and Formulation of Nanoparticles Extract of Bundung Plant (Actinoscirpus grossus) with Variations in Concentration of Chitosan and Na-TPP Bases Using the Ionic Gelation Method. Jurnal Surya Medika, 8(3), 25–29. https://doi.org/10.33084/jsm.v8i3.4495 DOI: https://doi.org/10.33084/jsm.v8i3.4495
Yulandasari, V., Hardiansah, Y., Husen, L. M. S., Mastuty, A., & Azhari, A. P. (2023). Education on the use Of Blu-Pea Flowers as a Herbal Plant in Mertak Tombok Village, Central Lombok. Community Development Journal : Jurnal Pengabdian Masyarakat, 4(6), 12453–12457. https://doi.org/10.31004/cdj.v4i6.22472
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