Pengaruh Metode Isolasi a-mangostin dari Kulit Buah Manggis (Garcinia mangostana L.) terhadap Rendemen α-mangostin

Authors

Sri Idawati , Aliefman Hakim , Yayuk Andayani

DOI:

10.29303/jppipa.v5i2.167

Published:

2019-04-18

Issue:

Vol. 5 No. 2 (2019): July

Research Articles

Downloads

How to Cite

Idawati, S., Hakim, A., & Andayani, Y. (2019). Pengaruh Metode Isolasi a-mangostin dari Kulit Buah Manggis (Garcinia mangostana L.) terhadap Rendemen α-mangostin. Jurnal Penelitian Pendidikan IPA, 5(2), 144–148. https://doi.org/10.29303/jppipa.v5i2.167
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Abstract: The purpose of this research is to know the effect of the isolation method of the yield of α-mangostin resulting from mangosteen rind. Mangosteen (Garcinia mangostana L.) is the source of xanthone secondary metabolites. Derivatives xanthone which is the main bioactive compound and becomes a major compound in the mangosteen plant, namely α-mangostin. This main compound has been isolated from ethanol extract on mangosteen rind using different extraction methods namely maceration and soxhletation. Purification was done by the recrystallization method with solvent ethanol: aquades (1:1). The Maceration extraction method produce the yields of extract and the yields of pure compounds (α-mangostin) is higher than that of soxhletation. The Maceration extraction method produces yields of extract 30,12% and yields of α-mangostin 4,27% while the soxhletation extraction method produce the yields of extract 26,34% and the yields of α-mangostin 2,13%. This yellow powder isolate has a melting point of 179,7 ºC. Purification is monitored by thin layer chromatography (TLC) and structural elucidation is carried out using FT-IR spectroscopy data.

 

Key Words: Isolation, α-mangostin, Garcinia mangostana

References

Ahmat, N., Azmin, N.F.N., Ab Ghani, N., Aris, S.R.S. 2010. Bioactive Xanthones from the Pericarp of Garcinia mangostana. Middle-East Journal of Scientific Research: 6(2), Malaysia, 123-127.

Andayani, R., Novita, R dan Verawati. 2015. Pengaruh Metode Ekstraksi terhadap Kadar Xanton Total dalam Ekstrak Kulit Buah Manggis Matang (Garcinia mangostana L.). Prosiding Seminar Nasional & Workshop “Perkembangan Terkini Sains Farmasi & Klinik 5†6-7 November 2015, Universitas Andalas, Padang.

Chae, H.-S., Oh, S.-R., Lee, H.-K., Joo, S.H., Chin, Y.-W., 2012. Mangosteen xanthones, α -and γ-mangostins, inhibit allergic mediators in bone marrow-derived mast cell. Food Chem. 134:397–400.

Ee., GCL., Daud, S., Izzaddin, SA dan Rahmare, M. 2008. Garcinia mangostana: a source of potential anti-cancer lead compounds against CEM-SS cell line. J Asian Nat Prod Res 10(5):475-479.

Hakim, A. 2016. Meningkatkan Kualitas Pembelajaran Kimia Bahan Alam Melalui Praktikum. Mataram: Arga Puji Press.

Keiser, J., Vargas, M., Winter, R., 2012. Anthelminthic properties of mangostin and mangostin diacetate. Parasitol. Int. 61:369–371.

Koh, J-J., Qiu, S., Zou, H., Lakshminarayanan, R., Li, J., Zhou, X., Tang, C., Saraswathi, P., Verma, C., Tan, D.T.H., Tan, A.L., Liu, S., Beuerman, R,W. 2013. Rapid Bactericidal Action of Alpha-Mangostin against MRSA as An Outcome of Membrane Targeting. Biohimica et Biophysica Acta.1828:834-844.

Kumar, Pradeep S.V., Puranik, B and Nandini, B.N. 2017. Evaluation of Alpha-Mangostin, Isolated and Purified from the Crude Extract of Garcinia mangostana for the Anti-Diabetic, Anti-inflamtory and Antioxidant Avtivity. Human Journals. 8(2):76-95.

Larson, R.T., Lorch, J.M., Pridgeon, J.W., Becnel, J.J., Clark, G.G., Lan, Q., 2010. The biological activity of alpha-mangostin, a larvicidal botanic mosquito sterol carrier protein-2 inhibitor. J. Med. Entomol. 47:249–257.

Manitto, P. 1992. Biosintesis Produk Alami. Penerjemah Koensoenardiyah, IKIP Surabaya.

Miryanti, A., Sapei, L., Budiono, K., Indra, S. 2011. Ekstraksi Antioksidan Dari Kulit Buah Manggis (Garcinia mangostana L.). Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Katolik Parahyangan Bandung.

Moongkarndi, P., Kosem, N., Kaslungka, S., Luanratana, O., Pongpan, N., Neungton, N. 2004. Antiproliferation, Antioxidant and Induction Of Ap(optosis By Garcinia mangostana mangosteen) on SKBR3 Human Breast Cancer Cell Line. J Ethnopharmacol. 90(1):66-161.

Mutmainnah, P.A., Hakim, A., Savalas, L.R.T. 2017. Identifikasi Senyawa Turunan Hasil Fraksinasi Kayu Akar Artocarpus Odoratissimus. Jurnal Penelitian Pendidikan IPA (JPPIPA). 3(2), Juli 2017.

Obolskiy, D., P, Ivo., S, Nisarat., H, Michael. 2009. Garcinia mangostana L: A phytochemical and Pharmacological Review. Phytoter. Res. 23 : 1047-1065.

Putri, I.P. 2015. Effectivity of Xanthone Of Mangosteen (Garcinia mangostana L.) Rind As Anticancer. J Majority. 4(1):33-38.

Quan, X., Wang, Y., Liang, Y., Tian, W., Ma, Q., Jiang, H., Zhao, Y., 2012. α -Mangostin induces apoptosis and suppresses differentiation of 3T3–L1 cells via inhibiting fatty acid synthase. PLoS One 7:33376–33379.

Sanchez-Perez, Y., Morales-Barcenas, R., Garcıa-Cuellar, C.M., Lopez-Marure, R., Calderon-Oliver, M., Pedraza-Chaverri, J., Chirino, Y.I., 2010. The α-mangostin prevention on cisplatin-induced apoptotic death in LLC-PK1 cells is associated to an inhibition of ROS production and p53 induction. Chem. Biol. Interact. 188:144–150.

Shankaranarayan, D., Gopalakhrishnan, C., kameswaran, L. 1979. Pharmacological profile of mangostin and its derivatives. Arch Internet Pharmaco Therapie. 239: 257-269.

Suksamrarn, S., Suwannapoch, N., Pakhodee, W., Thanuhiranlert, J., Ratnanukul, P., Chimnoi, N and Suksamrarn, A. 2003. Antimycro-bacterial Activity of Prenylated-xanthones From the Fruits of Garcinia mangostana. Chem Pharm Bull (Tokyo). 51(7):857-859.

Syamsudin., Farida., Widowati, D., Faizatun. 2008. Profil Distribusi dan Eliminasi Senyawa α-Mangostin setelah Pemberian Oral pada Tikus. Jurnal Sains dan Teknologi Farmasi. 13(2):53-58

Author Biographies

Sri Idawati, Politeknik Medica Farma Husada

Aliefman Hakim, Program Studi Magister Pendidikan IPA Pascasarjana Universitas Mataram

Yayuk Andayani, Program Studi Magister Pendidikan IPA Pascasarjana Universitas Mataram

License

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).