Bacterial Culture Streak Method for Inventory of Enterobacteriaceae Causes Diarrhea in the Body of House Fly (Musca Domestica)

Authors

Urip Urip , Titin Emilia Nirmala Sari , Maruni Wiwin Diarti , Erlin Yustin Tatontos

DOI:

10.29303/jppipa.v9i1.2513

Published:

2023-01-31

Issue:

Vol. 9 No. 1 (2023): January

Keywords:

Bacteria, Diarrhea, Flies, Streak method

Research Articles

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Urip, U., Sari, T. E. N. ., Diarti, M. W. ., & Tatontos, E. Y. . (2023). Bacterial Culture Streak Method for Inventory of Enterobacteriaceae Causes Diarrhea in the Body of House Fly (Musca Domestica). Jurnal Penelitian Pendidikan IPA, 9(1), 472–477. https://doi.org/10.29303/jppipa.v9i1.2513
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Abstract

Diarrhea is an endemic disease in Indonesia and is a potential disease Extraordinary Events (KLB) which are often accompanied by death. Based on data on diarrhea cases at the Narmada Community Health Center in 2017 there were 1.679 cases of diarrhea from 11 villages in Narmada District. One of the causes of these cases of diarrhea is the presence of house flies as a vector carrying disease. The purpose of this study was to determine the bacterial culture of the streak method for inventory of Enterobacteriaceae of diarrhea in the body of the house fly (Musca domestica). This research is descriptive observational. The sample in this study were 30 Musca domestica which were captured in the Kramajaya village environment in Nutrienth Agar Plate (NAP) and Mac Conkey Agar (MCA) media as well as the INVIC MUTSI Biochemical Test media on bacterial colonies that needed further identification to determine species. The analysis of the results was carried out descriptively. The identification results were found in the family Enterobacteriaceae genus Enterobacter agglomerans in the body of the house fly (Musca domestica) samples taken from the landfill and the family Moraxellaceae family genus Acinetobacter baumannii taken from the kitchen and home terrace

References

BPS Lombok Barat. (2020). Kabupaten Lombok Barat Dalam Angka 2020. Lombok Barat. Retrieved from https://lombokbaratkab.bps.go.id/publication/2020/05/20/2bb5920195530daa7ddd9b31/kabupaten-lombok-barat-dalam-angka-2020.html

Chandra, B. (2007). Disease Vector. Introduction to Environmental Health, 15–33.

Darna, D., Turnip, M., & Rahmawati, R. (2018). Deteksi dan Identifikasi Bakteri Anggota Enterobacteriaceae pada Makanan Tradisional Sotong Pangkong. Jurnal Labora Medika, 2(2), 6-12. Retrieved from https://jurnal.unimus.ac.id/index.php/JLabMed/article/view/3838

Depkes RI. (1999). Keputusan Menteri Kesehatan RI Nomor: 829/Menkes/SK/VII/1999 Tentang Persyaratan Kesehatan Perumahan. Jakarta: Departemen Kesehatan RI. Retrieved from https://bahan-ajar.esaunggul.ac.id/tpl313/wp-content/uploads/sites/996/2019/11/TPL306-Pembangunan-Perumahan-Pertemuan-3.pdf

Dharmawan, A. & Layanto, N. (2018). Mekanisme Resistensi Acinetobacter baumannii terhadap Antibiotik Golongan Karbapenem. Jurnal Kedokteran Meditek, 24(68), 68. https://doi.org/10.36452/jkdoktmeditek.v24i68.1704

Jawetz, Melnick & Adelberg’s. (2007). Medical Microbiology 24th Edition. Sultan Qaboos University Medical, 7(3), 273-275. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074881/

Mardaneh, J., Dallal., Mohammad, MS. (2013). Isolation, identification and antimicrobial susceptibility of Pantoea (Enterobacter) agglomerans isolated from consumed powdered infant formula milk (PIF) in NICU ward: First report from Iran. Iranian journal of microbiology, 5(3), 263-267. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895565/

Noorhamdani. (2004). Aktivitas Hemaglutinasi Bakteri Acinetobacter Baumannii Yang Berasal Dari Spesimen Klinik Dan Lingkungan. Jurnal Kedokteran Brawijaya, 20(2), 105-109. https://doi.org/10.21776/ub.jkb.2004.020.02.10

Nudyanto, A., & Zubaidah, E. (2015). Isolasi Bakteri Asam Laktat Penghasil Eksopolisakarida Dari Kimchi. Jurnal Pangan dan Agroindustri, 3(2), 743–748. Retrieved from https://jpa.ub.ac.id/index.php/jpa/article/view/195

Pertiwi, N. T. Y., & Budayanti, N. N. S. (2018). Studi Molekuler Gen Oxa-23 Pada Isolat Bakteri Acinetobacter Baumannii Resisten Terhadap Antibiotik Karbapenem di RSUP Sanglah Denpasar. Jurnal Medika Udayana, 7(6). Retrieved from https://ojs.unud.ac.id/index.php/eum/article/view/41451

Prabowo K. (1992). Petunjuk Praktis Pengendalian Vektor dan Binatang Pengganggu. Jakarta: Departemen Kesehatan RI.

Purwoko, T. (2007). Fisiologi Mikroba. Jakarta: Bumi Aksara.

Putri, YP. (2018). Identifikasi Bakteri Pada Tubuh Lalat Rumah (Musca Domestica Linn.) di Tempat Pembuangan Akhir Sampah (TPA) dan Pasar. Jurnal Biota, 4(1), 29-35. https://doi.org/10.19109/Biota.v4i1.1626

Safitri, V., Hastutiek, P., & Arimbi. (2017). Identification of Bacteria on the Fly Exoskeleton in Some Markets in Surabaya. Journal of Parasite Science (JoPS), 1(1), 1-6. https://doi.org/10.20473/jops.v1i1.16232

Setiyorini, E. (2017). Identifikasi Salmonella sp. Pada Lalat Rumah (Musca domestica) Dan Lalat Hijau (Chrysomya megachepala) Di Pasar Legi Citra Niaga Jombang. Doctoral dissertation, STIKES Insan Cendekia Medika Jombang.

Scerpella, EG., Wanger, AR., Armitige, L., Anderlini, P., Ericsson, CD. (1995). Nosocomial Outbreak Caused by a Multiresistent Clone of: Results of Case Control and Molecular Epidemiologic Investigations. Infection Control & Hospital Epidemiology, 16(2), 92-97. https://doi.org/10.2307/30140949

Sharma, M. (2012). Multidrug Resistant Pantoea agglomerans In a Patient with Septic Arthritis- a Rare Report from India. International Journal of Microbiology Research, 4(6), 263-265. Retrieved from https://pdfs.semanticscholar.org/ecb4/f496b8716ca1f81e60db47c8579e9c0b16b2.pdf

Tatontos, EY., Agrijanti., Inayati, N. (2017). Identifikasi Bakteri Basil Gram Negatif pada lalat Rumah (Musca domestica) di Pasar Abian Tubuh. Penelitian Mandiri. Poltekes Kemenkes. Mataram.

Tham, J. (2012). Extended-spectrum beta-lactamase-producing enterobacteriaceae: epidemiology, risk factors, and duration of carriage. Lund University.

Author Biographies

Urip Urip, Department of Health Analyst, Poltekkes Kemenkes Mataram

Titin Emilia Nirmala Sari, Poltekkes Kemenkes Mataram

Maruni Wiwin Diarti, Poltekkes Kemenkes Mataram

Erlin Yustin Tatontos, Politeknik Kesehatan Mataram

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