Characterization and Identification of Secondary Metabolite Compounds from Bacteria in Symbiont with Ascidia gemmate
DOI:
10.29303/jppipa.v9i8.3874Published:
2023-08-25Downloads
Abstract
Isolation of secondary metabolites from bacteria that have a symbiont with Ascidians has been shown to have enormous potential. This study aims to identify the types of secondary metabolites in Bacillus sp. which has a symbiont with A. gemmata. The research was carried out using laboratory experimental methods by means of phytochemical screening. After testing, the results of the characterization of bacteria that lead to the genus Bacillus and the secondary metabolites contained are alkaloid compounds
Keywords:
A. gemmate Alkaloids BacillusReferences
Abitua, P. B., Wagner, E., Navarrete, I. A., & Levine, M. (2012). Identification of a rudimentary neural crest in a non-vertebrate chordate. Nature, 492(7427), 104–107. https://doi.org/10.1038/nature11589
Amirkia, V., & Heinrich, M. (2014). Alkaloids as drug leads - A predictive structural and biodiversity-based analysis. Phytochemistry Letters, 10, xlviii–53. https://doi.org/10.1016/j.phytol.2014.06.015
Asolkar, R. N., Kirkland, T. N., Jensen, P. R., & Fenical, W. (2010). Arenimycin, an antibiotic effective against rifampin- and methicillin-resistant Staphylococcus aureus from the marine actinomycete Salinispora arenicola. Journal of Antibiotics, 63(1), 37–39. https://doi.org/10.1038/ja.2009.114
Bara, R. A., Kandou, G. D., Ola, A. R. B., & Posangi, J. (2015). Analisis Senyawa Antibiotik dari Jamur Simbion yang Terdapat dalam Ascidia Didemnum mole di Sekitar Perairan Bunaken-Sulawesi Utara. Jurnal LPPM Bidang Sains Dan Teknologi, 2(2), 28–35. Retrieved from https://ejournal.unsrat.ac.id/index.php/lppmsains/article/view/10688
Chen, L., Wang, X., Fu, C., & Wang, G. (2019). Phylogenetic Analysis and Screening of Antimicrobial and Antiproliferative Activities of Culturable Bacteria Associated with the Ascidian Styela clava from the Yellow Sea , China. Hindawi Biomed Research International, 2019, 1–14. https://doi.org/10.1155/2019/7851251
Debnath, B., Singh, W. S., Das, M., Goswami, S., Singh, M. K., Maiti, D., & Manna, K. (2018). Role of plant alkaloids on human health: A review of biological activities. Materials Today Chemistry, 9, 56–72. https://doi.org/10.1016/j.mtchem.2018.05.001
Furr, M., & Mahlberg, P. G. (1981). Histochemical analyses of laticifers and glandular trichomes in cannabis sativa. Journal of Natural Products, 44(2), 153–159. https://doi.org/10.1021/np50014a002
Goldstein, D., & Mayer, S. C. (1958). New Specific Test For Callcium. Analytica Chimica Acta, 19, 437–439. https://doi.org/10.1016/S0003-2670(00)88191-X
Hellio, C., Trepos, R., Aguila-Ramirez, N. R., & Hernandez-Guerrero, J. C. (2015). Protocol for Asessing Antifouling Activities of Macroalgal Extracts. Natural Products From Marine Algae: Methods and Protocols, 1308, 421–435. https://doi.org/10.1007/978-1-4939-2684-8
Hotta, K., Dauga, D., & Manni, L. (2020). The ontology of the anatomy and development of the solitary ascidian Ciona: the swimming larva and its metamorphosis. Scientific Reports, 10(1), 1–16. https://doi.org/10.1038/s41598-020-73544-9
Lichman, B. R. (2021). The scaffold-forming steps of plant alkaloid biosynthesis. Natural Product Reports, 38(1), 103–129. https://doi.org/10.1039/d0np00031k
Maciver, S. K., Evans, J., Borg, J. A., Ramos-Esplá, A. A., & Schembri, P. J. (2017). Status of the “Mangrove tunicate†Ecteinascidia turbinata (Ascidiacea: Perophoridae) in the Mediterranean Sea. Journal of the Marine Biological Association of the United Kingdom, 97(2), 369–376. https://doi.org/10.1017/S0025315416000473
Marliana, D. S., & Suyono, S. V. (2005). Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol. Biofarmasi, 3(1), 26–31. Retrieved from https://www.academia.edu/download/37761182/F030106.pdf
Mogea, R. (2015). Identifikasi Dan Uji Potensi Senyawa Antimikroba Dari Bakteri Simbion Ascidian. Universitas Brawijaya.
Morita, M., & Schmidt, E. W. (2018). Parallel lives of symbionts and hosts: Chemical mutualism in marine animals. Natural Product Reports, 35(4), 357–378. https://doi.org/10.1039/c7np00053g
Muthmainnah, B. (2017). Skrining Fitokimia Senyawa Metabolit Sekunder dari Ekstrak Etanol Buah Delima (Punica granatum L.) Dengan Metode Uji Warna. Media Farmasi Poltekkes Makassar, XIII(2), 1–6. https://doi.org/10.32382/mf.v13i2.880
Newman, D. J. (2016). Developing natural product drugs: Supply problems and how they have been overcome. Pharmacology and Therapeutics, 162, 1–9. https://doi.org/10.1016/j.pharmthera.2015.12.002
Noer, Z., Manik, P. J., & Sihotang, S. (2023). Rat Taro Extract (Typhonium flagelliforme (Lodd.)) as A Biofungicide Against the Cause of Leaf Fall (Pestalotiopsis sp.) on Rubber Plants (Hevea brasiliensis). Jurnal Penelitian Pendidikan IPA, 9(6), 4699–4704. https://doi.org/10.29303/jppipa.v9i6.3903
Olguin-Uribe, G., Abou-Mansour, E., Boulander, A., Débard, H., Francisco, C., & Combaut, G. (1997). 6-bromoindole-3-carbaldehyde, from an Acinetobacter Sp. Bacterium associated with the ascidian Stomozoa murrayi. Journal of Chemical Ecology, 23(11), 2507–2521. https://doi.org/10.1023/B:JOEC.0000006663.28348.03
Opa, S., Bara, R., Gerung, G., Rompas, R., Lintang, R., & Sumilat, D. (2018). Uji aktivitas antibakteri fraksi n-heksana, metanol dan air dari ascidian Lissoclinum sp. Jurnal Pesisir Dan Laut Tropis, 6(1), 69. https://doi.org/10.35800/jplt.6.1.2018.20566
Raal, A., Meos, A., Hinrikus, T., Heinämäki, J., RomÄne, E., GudienÄ—, V., JakÅ¡tas, V., Koshovyi, O., Kovaleva, A., Fursenco, C., Chiru, T., & Nguyen, H. T. (2020). Dragendorff’s reagent: Historical perspectives and current status of a versatile reagent introduced over 150 years ago at the University of Dorpat, Tartu, Estonia. Pharmazie, 75(7), 299–306. https://doi.org/10.1691/ph.2020.0438
Rinehart, K. L., Gloer, J. B., Hughes, R. G., Renis, H. E., Patrick McGovren, J., Swynenberg, E. B., Stringfellow, D. A., Kuentzel, S. L., & Li, L. H. (1981). Didemnins: Antiviral and antitumor depsipeptides from a Caribbean tunicate. Science, 212(4497), 933–935. https://doi.org/10.1126/science.7233187
Rinehart, K. L., Holt, T. G., Fregeau, N. L., Stroh, J. G., Keifer, P. A., Sun, F., Li, L. H., & Martin, D. G. (1990). Ecteinascidins 729, 743, 745, 759A, 759B, and 770: Potent Antitumor Agents from the Caribbean Tunicate Ecteinascidia turbinata. Journal of Organic Chemistry, 55(15), 4512–4515. https://doi.org/10.1021/jo00302a007
Sahuma, E. P., Sumilat, D. A., Warow, V., Losung, F., Angkouw, E. D., & Kalesaran, O. (2021). Potensi Bioaktivitas Anti Jamur dan Anti-UV dari Isolat Jamur Simbion Pada Ascidia Eudistoma sp. Jurnal Pesisir Dan Laut Tropis, 9(3), 74. https://doi.org/10.35800/jplt.9.3.2021.36661
Saidi, N., Ginting, B., Murniana, & Mustanir. (2018). Analisis Metabolit Sekunder (Pertama). Syiah Kuala University Press Darussalam. Retrieved from https://www.google.co.id/books/edition/Analisis_Metabolis_Sekunder/sJHPDwAAQBAJ?hl=en&gbpv=1&dq=uji+fitokimia&printsec=frontcover
Saifudin, A. (2014). Senyawa Alam Metabolit Sekunder (1st ed.). Deepublish publisher.
Sathishkumar, R., Ananthan, G., Senthil, S. L., Meivelu, M., & Arun, J. (2018). Structural characterization and anticancer activity of extracellular polysaccharides from ascidian symbiotic bacterium Bacillus thuringiensis. Carbohydrate Polymers, 190, 113–120. https://doi.org/10.1016/j.carbpol.2018.02.047
Shenkar, N., Gittenberger, A., Lambert, G., Rius, M., Rocha, R. M. da, Swalla, B. J., & Turon, X. (2017). Ascidiacea World Database. World Register of Marine Species. Retrieved from https://marinespecies.org/ascidiacea/
Socha, A. M., Garcia, D., Sheffer, R., & Rowley, D. C. (2006). Antibiotic bisanthraquinones produced by a streptomycete isolated from a cyanobacterium associated with Ecteinascidia turbinata. Journal of Natural Products, 69(7), 1070–1073. https://doi.org/10.1021/np050449b
Sumilat, D. A., Wewengkang, D. S., Rotinsulu, H., Yamazaki, H., Oda, T., Ukai, K., & Namikoshi, M. (2018). Bioactivity of extracts from ascidians collected in North Sulawesi as seeds of marine-derived drugs. Aquaculture, Aquarium, Conservation & Legislation, 11(2), 516-524. Retrieved from https://www.proquest.com/openview/f7a86ffee0bffc5ac42a4f3f8ce1baa6/1?pq-origsite=gscholar&cbl=2046424
Suteja, A., Kardhinata, H. E., & Lubis, R. (2020). Identifikasi Senyawa Metabolit Sekunder Pada Durian (Durio zibetthinus Murr). Jurnal Ilmiah Biologi UMA (JIBIOMA), 1(1), 1–6. https://doi.org/10.31289/jibioma.v1i1.138
Tsukimoto, M., Nagaoka, M., Shishido, Y., Fujimoto, J., Nishisaka, F., Matsumoto, S., Harunari, E., Imada, C., & Matsuzaki, T. (2011). Bacterial production of the tunicate-derived antitumor cyclic depsipeptide didemnin B. Journal of Natural Products, 74(11), 2329–2331. https://doi.org/10.1021/np200543z
Xu, Y., Kersten, R. D., Nam, S. J., Lu, L., Al-Suwailem, A. M., Zheng, H., Fenical, W., Dorrestein, P. C., Moore, B. S., & Qian, P. Y. (2012). Bacterial biosynthesis and maturation of the didemnin anti-cancer agents. Journal of the American Chemical Society, 134(20), 8625–8632. https://doi.org/10.1021/ja301735a
Zulkifli, L., Jekti, D. S. D., & Bahri, S. (2018). Isolasi, Karakterisasi Dan Identifikasi Bakteri Endofit Kulit Batang Srikaya (Annona Squamosa) Dan Potensinya Sebagai Antibakteri. Jurnal Penelitian Pendidikan IPA, 4(1). https://doi.org/10.29303/jppipa.v4i1.98
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