Antidiabetic Activity of Ethanol-Water Fraction of Longjack Root (Eurycoma longifolia Jack.) in Streptozotocin-Nicotinamide Induced Rats

Authors

Wiwik Hartika , Michelle Terrence , Ikhsan Samir , Ruqiah Ganda Putri Panjaitan

DOI:

10.29303/jppipa.v10i2.4759

Published:

2024-02-25

Issue:

Vol. 10 No. 2 (2024): February

Keywords:

Longjack root, Blood glucose level, Diabetes mellitus, MDA levels, Pancreatic histopathology

Research Articles

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Hartika, W., Terrence, M., Samir, I., & Panjaitan, R. G. P. (2024). Antidiabetic Activity of Ethanol-Water Fraction of Longjack Root (Eurycoma longifolia Jack.) in Streptozotocin-Nicotinamide Induced Rats. Jurnal Penelitian Pendidikan IPA, 10(2), 776–783. https://doi.org/10.29303/jppipa.v10i2.4759
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Abstract

This study aims to determine the decrease in glucose levels, MDA levels, and histopathological appearance of the pancreatic organs of Wistar white rats after administration of the ethanol-water fraction of longjack root. This study used a completely randomized design with four treatment groups and six replications. The treatment consisted of normal control, negative control (Na-CMC 0.5% 5 ml/200 g body weight), positive control (glibenclamide 0.09 mg/200 g body weight), and treatment of the ethanol-water fraction of longjack root with a dose of 500 mg/kg body weight. The results showed that the ethanol-water fraction of longjack root at a dose of 500 mg/kg body weight had antidiabetic activity comparable to that of glibenclamide at a dose of 0.09/200 g body weight, namely blood glucose levels and MDA levels in white rats of 123.55 mg/dl and 2.83 nmol/ml. The results of histopathological analysis of the pancreas of white rats showed that administration of the ethanol-water fraction of longjack root could repair damage to the islets of Langerhans due to the administration of streptozotocin-nicotinamide.

References

Arief, H., & Widodo, M. A. (2018). Peranan Stress Oksidatif pada Proses Penyembuhan Luka. Jurnal Ilmu Kedokteran Wijaya Kusuma, 5(2), 22-29. http://dx.doi.org/10.30742/jikw.v5i2.338

Dompeipen, E. J., & Simanjuntak. (2015)Aktivitas Antidiabetes dan Antioksidan Kapang Endofit dari Tanaman Mahoni (Swietenia macrophylla King.). Biopropal Industri, 6(1), 7-17. http://dx.doi.org/10.36974/jbi.v6i1

Fatimah, R. N. (2015). Diabetes Mellitus Tipe 2. Jurnal Majority, 4(5), 93-101. Retrieved from https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/615/619

Firdaus, Rimbawan, Maliyati, S. A., & Roosita, K. (2016). Model Tikus Diabetes yang Diinduksi Streptozotocin-Sukrosa untuk Pendekatan Penelitian Diabetes Mellitus Gestational. Jurnal MKMI, 12(1), 29-34. https://doi.org/10.30597/mkmi.v12i1.550

Fransisca, Kalangi, G. E., Saptasari, D.C., & Hendra, P. (2018). The Effect of Pasak Bumi Roots Towards Blood Glucose Level in Glucose Loaded-Mice. Jurnal Farmasi Sains dan Komunitas, 15(1), 1-6. https://doi.org/10.24071/jpsc.00965

Gashemi, A., Khalifi, S., & Jedi, S. (2014). Streptozotocin-Nicotinamide-Induced Rat Model of Type 2 Diabetes (Review). Acta Physiologica Hungarica, 101(4), 408-420. https://doi.org/10.1556/APhysiol.101.2014.4.2

Gregory, M. M., Sung, M. W., Yang, Z., Innes, L. M., Kandasamy, B., David, L., Yoshioka, C., & Shyng, S. L. (2019). Mechanism of pharmacy chaperoning in a mammalian KATP channel revealed by cryo-EM. Journal Elife, 1(1), 1-26. https://doi.org/10.7554/eLife.46417

Hazni, R., Gustiawan, R., Zulfian, Lestari, S. M. P., Arania, R., & Sudiadnyani, N. P. (2021). Penyuluhan Diabetes Mellitus di Puskesmas Rawat Inap Sukaraja Bandar Lampung. Jurnal Kreativitas Pengabidan Kepada Masyarakat 4(1), 181-187. http://doi.org/10.33024/jkpm.v4i1.3728

Hidayati, S., Zuhud, E. A. M., Adiyaksa, I. K., & Manar, P. A. (2021). Review: Etnotaksonomi dan Bioteknologi Tumbuhan Pasak Bumi (Eurycoma longifolia Jack.). J of Natural Resources and Environmental Management 11(2), 177-188. http://dx.doi.org/10.29244/jpsl.11.2.177-188

Husan, F., Suyatna, F. D., Arozal, W., Purwaningsih, & E. H. 2019. Model Hewan Coba pada Penelitian Diabetes. Pharmaceutical Sciences and Research 6(3), 131-141. https://doi.org/10.7454/psr.v6i3.4531

IDF Diabetes Atlas. (2021) International Diabetes Federation (IDF), 10th Edition. Brussels: International Diabetes Federation. Retrieved from https://diabetesatlas.org/

Iryani, I., Iswendi, I., & Katrina, I. T. (2017). Uji Aktivitas Antidiabetes Mellitus Senyawa Metabolit Sekunder Fraksi Air dari Beras Ketan Hitam (Oryza satival. Var glutinosa) pada Mencit Putih. Eksakta, 18(1), 54-60. https://doi.org/10.24036/eksakta/vol18-iss01/17

Jurnalis, Y. D., Sayoeti, Y., & Elfitrimelly, E. (2014). Peran Antioksidan pada non Alcoholic Fatty Liver Disease (NAFLD). Jurnal Kesehatan Andalas, 3(1), 15-20. https://doi.org/10.25077/jka.v3i1.18

Khairani, Yuniarti, E., & Sumarmin, R. (2018). Pengaruh Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) terhadap Histopatologi Pankreas Mencit (Mus musculus L. Swiss Webster) yang Diinduksi Sukrosa. Eksakta, 19(1), 100-112. https://doi.org/10.24036/eksakta/vol19-iss1/123

Khajinou, T., & Jiraungkoorskul, W. (2016). Review The Ergogenic Effect of Long Jack (Eurycoma longifolia). Pharmacognosy Rev 10(20), 139-141. http://doi.org/10.4103/0973-7847.194041

Liem, S., Yuliet, & Khumaidi, A. (2015). Uji Aktivitas Antidiabetes Kombinasi Glibenklamid Dan Ekstrak Daun Salam (Syzygium Polyanthum Wight.) Terhadap Mencit (Mus Musculus) Yang Diinduksi Aloksan. Galenika Journal of Pharmacy, 1(1), 42-47. https://doi.org/10.22487/j24428744.2015.v1.i1.4831

Miniawy, H. M. F. E. L., Ahmed, K.A., Ibrahem, E. M., Sabry, D., Tahany, A., Ismail, & Amer, E. (2017). Camel’s Wharton Jelly Mesenchymal Stem Cell is a Novel Tool for Regeneration of Induced Diabetes Mellitus. Journal of Translational Science, 3(5), 1-6. http://doi.org/10.15761/JTS.1000196

Mohamed, A. N., Vejayan, J., & Yusoff, M. M. (2015). Review on Eurycoma longifolia Pharmacological and Phytochemical Properties. Journal of Applied Sciences, 15(6), 1-14. http://doi.org./10.3923/jas.2015.831.844.

Nuralifah, Fitrawan, L. O. M., Parawansah, & Trisetya M. (2022). Histopatologi Organ Pankreas Tikus DM tipe 2 yang diberi Ekstrak Daun Gedi Merah (Abelmoscus manihot L. Medik). Journal Syifa Sci and Clinical Research, 4(1), 141-151. https://doi.org/10.37311/jsscr.v4i1.13566

Oktavia, F., Erianto, M., & (2020). Pola Penderita Karsinoma Pankreas di RSUD Dr. H. Abdul Moeloek. Jurnal Ilmiah Kesehtan Sandi Husada, 9(2), 741-747. https://doi.org/10.35816/jiskh.v12i2.393

Panjaitan, R. G. P., & Astuti, A. (2021). Antidiabetic Activity of The Leaf Extract of Eurycoma longifolia Jack. in Streptozotocin-Nicotinamide Induced Diabetic Model. Pharmacogn Journal, 13(6), 1582-1588. http://doi.org/10.5530/pj.2021.13.203.

Panjaitan, R. G. P., Handharyani, E., Zakiah Z, Manalu W. 2013. Hepatoprotective Activity of Eurycoma longifolia Jack. Roots. Indian J of Traditional Knowledge, 12(2), 225-230. Retrieved from https://nopr.niscpr.res.in/handle/123456789/16865

Panjaitan, R. G. P., & Novitasari. (2021). Anti-diabetic Activity of the Red Dragon Fruit Peel (Hylocereus polyrhizus) in Ethanol Extract Againts Diabetic Rats. Pharmacognosy Journal, 13(5), 1079-1085. http://doi.org/10.5530/pj.2021.13.140

Prakosa, A. G., Ratnawati, R., & Prabawati, R. K. (2017). Pengaruh Antosianin Ungu (Ipomoea batatas L.) Kultivar Gunung Kawi terhadap Ekspresi Caspase-3 pada Jaringan Otak Tikus Model DM Tipe 2. Majalah Kesehatan, 4(2), 52-58. https://doi.org/10.21776/ub.majalahkesehatan.2017.004.02.1

Puspitasari, S., & Syauqy, A. (2015). Pengaruh Pemberian Pisang Kepok (Musa paradisiaca Forma Typical) terhadap Kadar Malondialdehid (MDA) Tikus Sprague Dawley Pra-Sindrom Metabolik. Journal of Nutrition College, 4(2), 314-322. https://doi.org/10.14710/jnc.v4i4.10101

Putri, E. P., Gofur, A., & Lestari, S. R. (2017). Pengaruh Variasi Campuran Ekstrak Tempe Kedelai Hitam dan Ubi Jalar Ungu terhadap Aktivitas Lipid Perozidation dan Reactive Oxygen Species (ROS) Total pada Tikus Model Diabetes Mellitus Tipe 2. Jurnal Ilmu Hayati, 1(2), 76-85. http://dx.doi.org/10.17977/um061v1i22017p76-85

Rantung, J., Yetti K., & Herawati, T. (2015). Hubungan Self-Care dengan Kualitas Hidup Pasien Diabetes Mellitus (DM) di Persatuan Diabetes Indonesia (PERSADIA) Cabang Cimahi. Jurnal Skolastik Keperawatan, 1(1), 38-51. https://doi.org/10.35974/jsk.v1i01.17

Solfaine, R., Muniroh, L., & Mubarokah, W. W. (2019). Efek Ekstrak Daun Tithonia diversifolia terhadap Penurunan Konsentrasi Adiponektin pada Tikus Diabetik yang Diinduksi oleh Streptozotocin. Jurnal Sains Veteriner, 37(2), 143-150. https://doi.org/10.22146/jsv.46837

Suastuti, N. G.A M. D.A., Dewi, I. G. A. K. S. P., & Ariati, N. K. (2015). Pemberian Ekstrak Daun Sirsak (Annona muricuta) untuk Memperbaiki Kerusakan Sel Beta Pankreas Melalui Kadar Glukosa Darah, Advanced Glycation, and Product, dan 8-hidroksi-2-dioksiguanosin pada Tikus Wistar Hiperglikemia. Jurnal Kimia 9(2), 289-295. https://doi.org/10.24843/JCHEM.2015.v09.i02.p21

Suryani, N., Endang, T., & Aulanni’am. (2014). Pengaruh Ekstrak Metanol Biji Mahoni terhadap Peningkatan Kadar Insulin, Penurunan Ekspresi TNF-α dan Perbaikan Jaringan Pankreas Tikus Diabetes. Jurnal Kedokteran Brawijaya, 27(3), 137-145. https://doi.org/10.21776/ub.jkb.2013.027.03.3

Verma, S., Gupta, M., Popli, H., & Aggarwali, G. (2018). Diabetes Mellitus Treatment Using Herbal Drugs. International Journal of Phytomedicine, 10(1), 1-10. http://doi.org/10.5138/09750185.2181

Walean, M., Melpin. R., Rondonuwu. M., & Pinontoan, K. F. (2020). Perbaikan Histopatologi Pankreas Tikus Hiperglikemia setelah Pemberian Ekstrak Etanol Kulit Batang Pakoba (Syzygium luzonense (Merr.) Merr.). Majalah Ilmiah Biologi Biosfera: A Scientific Journal, 37(1), 43-48. http://doi.org/10.20884/1mib.2020.37.1.1210

Wiendarlina, I. Y., Rahminiwati, M., & Gumelar, F. T. (2018). Aktivitas Hepatoprotektor Ekstrak Air Herba Pegagan Daun Kecil (Centella asiatica L. Urb.) terhadap Tikus Putih Jantan Sprague Dawley L. yang Diinduksi dengan Parasetamol. Fitofarmaka. Jurnal Ilmiah Farmasi, 8(1), 13-24. http://doi.org/10.33751/jf.v8i1.1167

Zakaria, A., Yahya, Z., & Nurmayunita, H. (2019). Pengaruh Pemberian The Daun Tin terhadap Kadar Gula Darah pada Penderita Diabetes Mellitus. Jurnal Ilmu Kesehatan, 7(2), 357-365. https://doi.org/10.32831/jik.v7i2.215

Author Biographies

Wiwik Hartika, Tanjungpura University

Michelle Terrence, Tanjungpura University

Ikhsan Samir, Tanjungpura University

Ruqiah Ganda Putri Panjaitan, Tanjungpura University

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Copyright (c) 2024 Wiwik Hartika, Michelle Terrence, Ikhsan Samir, Ruqiah Ganda Putri Panjaitan

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