Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method
DOI:
10.29303/jppipa.v7i2.675Published:
2021-04-22Downloads
Abstract
Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidantKeywords:
Antidesma bunius L Spreng Phytochemical Screening AntioxidantsReferences
Amarowicz, R. (2007). Tanin : The New Natural Antioxidants? European Journal of Lipid Science and Technology, 109(6), 549–551. doi: https://doi.org/10.1002/ejlt.200700145
Departemen Kesehatan Republik Indonesia. (1977). Materia Medika Indonesia Jilid I-IV. Jakarta: Departemen Kesehatan Republik Indonesia. [Indonesian]
Elya, B., Basah, K., Mun'im, A., Yuliastuti, W., Bangun, A., & Septiana, E. K. (2012). Screening of α-glucosidase inhibitory activity from some plants of Apocynaceae, Clusiaceae, Euphorbiaceae, and Rubiaceae. Journal of biomedicine & biotechnology, 2012, 281078. doi: https://doi.org/10.1155/2012/281078
Habibi, A. I., Firmansyah, A., & Setyawati, S. M. (2018). Skrining Fitokimia Ekstrak n-Heksan Korteks Batang Salam (Syzygium polyanthum). Indonesian Journal of Chemical Science, 7(1), 1–4. Retrieved from: https://journal.unnes.ac.id/sju/index.php/ijcs/article/view/23370 [Indonesian]
Handayani, V., Ahmad, A., & Sudir, M. (2016). Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH. Pharmaceutical Sciences and Research (PSR), 1(2), 86-93. doi: 10.7454/psr.v1i2.3321. [Indonesian]
Harborne, J.B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: ITB. [Indonesian]
Hardiningtyas, S. D., Purwaningsih, S., & Handharyani, E. (2014). Aktivitas Antioksidan dan Efek Hepatoprotektif Daun Bakau Api-Api Putih. Jurnal Pengolahan Hasil Perikanan Indonesia, 17(1), 80–91. doi: https://doi.org/10.17844/jphpi.v17i1.8140 [Indonesian]
Indarto. (2015). Uji kualitatif dan kuantitatif golongan senyawa organik Dari kulit dan kayu batang tumbuhan Artocarpus dadah Miq. Jurnal Ilmiah Pendidikan Fisika Al-Biruni, 4(1), 75–84. doi: https://doi.org/10.24042/jpifalbiruni.v4i1.82 [Indonesian]
Islam, S., Munira, S., Zaman, S., Ferdowsy Koly, S., Sukorno, F. I., Ahammed, S., & Hridoy, R. azmir. (2018). Estimation of phytochemical, antioxidant screening profile and thrombolytic activities of methanolic extract of Antidesma bunius L. leaf. Horticulture International Journal, 2(6), 358–363. doi: https://doi.org/10.15406/hij.2018.02.00077
Kurniati, R.I. (2013). Kurniati, ruth indah. 2013. Uji Aktivitas Antioksidan Fraksi Etanol Daun Buas-Buas (Premna cordifolia Linn.) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 3(1), 1–13. Retrieved from: https://jurnal.untan.ac.id/index.php/jmfarmasi/article/view/3820 [Indonesian]
Lung, J. K. S., & Destiani, D. P. (2017). Uji Aktivitas Antioksidan Vitamin A, C, E dengan Metode DPPH. Farmaka, 15(1), 53–62. [Indonesian]
Marliana, E. (2012). Aktivitas Antioksidan Ekstrak Etanol Daun Andong (Cordyline fruticosa L) A. Cheval. Mulawarman Scientifie, 11(1). [Indonesian]
Maulida, W., Fadraersada, J., & Rijai, L. (2016). Isolasi Senyawa Antioksidan Dari Daun Pila-Pila (Mallotus paniculatus). Proceeding of Mulawarman Pharmaceuticals Conferences, 4(1), 384–390. [Indonesian]
Mustarichie, R., Musfiroh, I., & Levita, J. (2011). Metode Penelitian Tanaman Obat. Bandung: Widya Padjajaran. [Indonesian]
Nugrahani, R., Andayani, Y., & Hakim, A. (2016). Skrining Fitokimia dari Ekstrak Buah Buncis (Phaseolus Vulgaris L.) dalam Sediaan Serbuk. Jurnal Penelitian Pendidikan Ipa, 2(1), 35–42. doi: https://doi.org/10.29303/jppipa.v2i1.38 [Indonesian]
Onuekwusi, E. C., Akanya, H. O., & Evans, E. C. (2014). Phytochemical Constituents of Seeds of Ripe and Unripe Blighia Sapida (K. Koenig) and Physicochemical Properties of the Seed Oil. International Journal of Pharmaceutical Science Invention, 3(9), 31–40.
Permadi, A., Sutanto, & Wardatun, S. (2015). Perbandingan Metode Ekstraksi Bertingkat Dan Tidak Bertingkat Terhadap Flavonoid Total Herba Ciplukan Secara Kolorimetri. Jurnal Online Mahasiswa (JOM) Bidang Farmasi, 1(1), 1–10. [Indonesian]
Phaniendra, A., & Babu, D. (2015). Free Radicals : Properties , Sources , Targets , and Their Implication in Various Diseases. 30(1), 11–26. doi: https://doi.org/10.1007/s12291-014-0446-0
Prayoga, dewa gede eka, Nocianitri, K. A., & Puspawati, N. N. (2019). Identifikasi Senyawa Fitokimia dan Aktivitas Antioksidan Ekstrak Kasar Daun Pepe (Gymnema reticulatum Br.) pada Berbagai Jenis Pelarut. Jurnal Ilmu Dan Teknologi Pangan, 8(2), 111–121. doi: https://doi.org/10.24843/itepa.2019.v08.i02.p01 [Indonesian]
Rabima, & Marshall. (2017). Uji Stabilitas Formulasi Sediaan Krim Antioksidan Ekstrak Etanol 70% dari Biji Melinjo (Gnetum gnemon L.). Indonesian Natural Research Pharmaceutical Journal, 2(1), 107–121. Retrieved from: http://journal.uta45jakarta.ac.id/index.php/INRPJ/article/view/834 [Indonesian]
Ramadenti, F., Sundaryono, A., & Handayani, D. (2017). Uji Fraksi Etil Asetat Daun Peronema canescens Terhadap Plasmodium berghei Pada Mus musculus. Alotrop, 1(2), 94–97. Retrieved from:https://ejournal.unib.ac.id/index.php/alotropjurnal/article/view/3508 [Indonesian]
Sangi, meiske S., Momuat, L. I., & Kumaunang, M. (2012). Uji Toksisitas dan Skrining Fitokimia Tepung Gabah Pelepah Aren (Arenga pinnata). Jurnal Ilmiah Sains, 12(2), 127–134. doi: https://doi.org/10.35799/jis.12.2.2012.716 [Indonesian]
Selamoglu, Z., Amin, K., Ugur, S., & Ozgen, O. (2018). Plant Secondary Metabolites With Antioxidant Properties And Human Health.
Sofwan, R. (2013). Bugar Selalu di Tempat Kerja. Jakarta: PT Bhuana Ilmu Populer. [Indonesian]
Susanti, N. M. P., I.N.A., B., & N.K, W. (2014). Skrining Fitokimia Ekstrak Etanol 90 % Daun Katuk (Sauropus androgynus (L.) Merr.). Jurnal Universitas Udayana, 3(1), 83–86. Retrieved from https://ojs.unud.ac.id/index.php/jfu/article/view/12035 [Indonesian]
Tandi, J., Melinda, B., Purwantari, A., & Widodo, A. (2020). Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L. Moench) dengan Metode Spektrofotometri UV-Vis. KOVALEN, 6(1), 74–80. doi: https://doi.org/10.22487/kovalen.2020.v6.i1.15044 [Indonesian]
Tjitda, P. J. P., & Nitbani, F. O. (2019). Skrining Fitokimia Ekstrak Metanol, Kloroform dan n-Heksan Daun Flamboyan (Delonix regia. Raf) Asal Kupang. Sains Dan Terapan Kimia, 13(2), 70–79. doi: http://dx.doi.org/10.20527/jstk.v13i2.5949 [Indonesian]
Wulansari, A. N. (2018). Alternatif Cantigi Ungu (Vaccinium varingiaefolium) sebagai Antioksidan Alami : Review. Farmaka, Universitas Padjadjaran, Bandung, 16(2), 419–429. doi: https://doi.org/10.24198/jf.v16i2.17574 [Indonesian]
Yuhernita, & Juniarti. (2011). Analisis Senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian yang Berpotensi sebagai Antioksidan. MAKARA SAINS, 15(1), 48–52. doi: https://doi/org/10.7454/mss.v15i1.877 [Indonesian]
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