Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method

Authors

Dewa Ayu Puspaning Kumaradewi , Windah Anugrah Subaidah , Yayuk Andayani , Ali Al-Mokaram

DOI:

10.29303/jppipa.v7i2.675

Published:

2021-04-22

Issue:

Vol. 7 No. 2 (2021): April

Keywords:

Antidesma bunius L, Spreng, Phytochemical Screening, Antioxidants

Research Articles

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How to Cite

Kumaradewi, D. A. P., Subaidah, W. A., Andayani, Y., & Al-Mokaram, A. (2021). Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method. Jurnal Penelitian Pendidikan IPA, 7(2), 275–280. https://doi.org/10.29303/jppipa.v7i2.675
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Abstract

Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidant

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Author Biographies

Dewa Ayu Puspaning Kumaradewi, Pharmacy Department, Faculty of Medicine, Mataram University, Mataram

Windah Anugrah Subaidah, Pharmacy Department, Faculty of Medicine, Mataram University, Mataram

Yayuk Andayani, SCOPUS ID : 57195057388, Universitas Mataram, Magister of Science Education Program

SCOPUS ID: 57195057388

Ali Al-Mokaram, Department of Chemistry, College of Science, Mustansiriyah University, 10052, Baghdad

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