The Effect of Papaya Leaf Infusion on Nile Tilapia Infected with Aeromonas hydrophila

Authors

Dian Puspitasari , Jeki , Intan Zahar

DOI:

10.29303/jppipa.v10i5.6852

Published:

2024-05-25

Issue:

Vol. 10 No. 5 (2024): May

Keywords:

Disease, Nile tilapia, Phytochemicals, Weight gain

Research Articles

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Puspitasari, D., Jeki, & Zahar, I. (2024). The Effect of Papaya Leaf Infusion on Nile Tilapia Infected with Aeromonas hydrophila. Jurnal Penelitian Pendidikan IPA, 10(5), 2416–2422. https://doi.org/10.29303/jppipa.v10i5.6852

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Abstract

This study aimed to determine the effectiveness of papaya leaf infusion against the weight gain of Nile tilapia infected with Aeromonas hydrophila and identify the active compounds contained therein.This study used a complete randomized design. The treatment consists of 5 treatments: treatment K (control), treatment A (25 ppt), treatment B (50 ppt), treatment C (75 ppt), and treatment D (100 ppt). This research includes several stages, the first being to infect tilapia with A. hydophila. The second stage is to mix papaya leaf infusion into the feed and observe behaviour, clinical symptoms, weight gain, water quality parameters, and phytochemical analysis. Results showed that treatment A showed the highest value for weight gain. Active compounds in papaya leaf infusion are alkaloids, flavonoids, terpenoids/steroids, glycosides, saponins, and tannins. Papaya leaf infusion increased the weight gain of Nile tilapia infected with A. hydrophila.

References

Abd El-Hack, M. E., El-Saadony, M. T., Nader, M. M., Salem, H. M., El-Tahan, A. M., Soliman, S. M., & Khafaga, A. F. (2022). Effect of environmental factors on growth performance of Nile tilapia (Oreochromis niloticus). In International Journal of Biometeorology (Vol. 66, Issue 11, pp. 2183–2194). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/s00484-022-02347-6

Adinew, G. M., Taka, E., Mendonca, P., Messeha, S. S., & Soliman, K. F. A. (2021). The anticancer effects of flavonoids through miRNAs modulations in triple-negative breast cancer. Nutrients, 13(4), 1–37. https://doi.org/10.3390/nu13041212

Ahmed, S. M., Hordofa, B., Meressa, B. H., & Tamiru, M. (2023). Population structure and genetic diversity of Nile tilapia (Oreochromis niloticus) using microsatellite markers from selected water bodies in southwest Ethiopia. Veterinary Medicine and Science, 9(5), 2095–2106. https://doi.org/10.1002/vms3.1212

Amer, S. A., El-Araby, D. A., Tartor, H., Farahat, M., Goda, N. I. A., Farag, M. F. M., Fahmy, E. M., Hassan, A. M., Abo El-Maati, M. F., & Osman, A. (2022). Long-Term Feeding with Curcumin Affects the Growth, Antioxidant Capacity, Immune Status, Tissue Histoarchitecture, Immune Expression of Proinflammatory Cytokines, and Apoptosis Indicators in Nile Tilapia, Oreochromis niloticus. Antioxidants, 11(5). https://doi.org/10.3390/antiox11050937

Candra, B., Nasmia, & Tobigo, D. T. (2017). Pengaruh Pemberian Enzim Papain Pada Pakan Buatan Terhadap Pertumbuhan dan Kelangsungan Hidup Udang Kaki Putih (Penaeus vannamei). J. Agrisains, 18(2), 77–83. Retrieved from http://jurnal.untad.ac.id/jurnal/index.php/AGRISAINS/article/view/16910/12091

Chotimah, I. H., Triatmoko, B., & Nugraha, A. S. (2020a). Skrining Fitokimia dan Uji Aktivitas Antibakteri Tumbuhan Jelutong Pipit (Kibatalia arborea (Blume) G.Don) terhadap Escherichia coli. Pharmaceutical Journal of Indonesia, 17(02), 493–500. https://doi.org/10.30595/pharmacy.v17i2.4806

Devmurari, V. V., Patel, P. P., Jadeja, R. A., Bhadaniya, C. P., Aghara, P. P., Patel, A. S., Tala, S. D., Savant, M. M., Ladva, K. D., & Nariya, P. B. (2021). Steroid and Fatty Acid Contents from the Leaves of Carica Papaya. Folia Medica, 63(3), 422–428. https://doi.org/10.3897/folmed.63.e55300

DirJen POM. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat (1st ed.). Departemen Kesehatan RI. Retrieved from https://www.academia.edu/10368669/Parameter_Standar_Umum_Ekstrak_Tumbuhan_Obat

Effendie, M. I. (1979). Metoda Biologi Perikanan. Yayasan Dewi Sri.

El-Garawani, I. M., Khallaf, E. A., Alne-na-ei, A. A., Elgendy, R. G., Sobhy, H. M., Khairallah, A., Hathout, H. M. R., Malhat, F., & Nofal, A. E. (2022). The Effect of Neonicotinoids Exposure on Oreochromis niloticus Histopathological Alterations and Genotoxicity. Bulletin of Environmental Contamination and Toxicology, 109(6), 1001–1009. https://doi.org/10.1007/s00128-022-03611-6

FAO. (2016). The State of World Fisheries and Aquaculture 2016 : Contributing to Food Security and Nutrition for All. FAO. Retrieved from https://www.fao.org/family-farming/detail/en/c/465805/

FAO. (2020). The State of World Fisheries and Aquaculture 2020. In INFORM (Vol. 32, Issue 6). American Oil Chemists Society. https://doi.org/10.4060/ca9229en

Gerrine, G., Prajitno, A., Fadjar, M., & Kenitasari, R. E. (2023). Effects of Keji Beling (Strobilanthes crispus) Crude Leaves Extract Against Aeromonas hydrophila Bacterial Infection in Vitro. Jurnal Penelitian Pendidikan IPA, 9(5), 3849–3855. https://doi.org/10.29303/jppipa.v9i5.3502

Hamid, N. K. A., Somdare, P. O., Md Harashid, K. A., Othman, N. A., Kari, Z. A., Wei, L. S., & Dawood, M. A. O. (2022). Effect of papaya (Carica papaya) leaf extract as dietary growth promoter supplement in red hybrid tilapia (Oreochromis mossambicus × Oreochromis niloticus) diet. Saudi Journal of Biological Sciences, 29(5), 3911–3917. https://doi.org/10.1016/j.sjbs.2022.03.004

Hariono, M., Julianus, J., Djunarko, I., Hidayat, I., Adelya, L., Indayani, F., Auw, Z., Namba, G., & Hariyono, P. (2021). The future of carica papaya leaf extract as an herbal medicine product. Molecules, 26(22). https://doi.org/10.3390/molecules26226922

Ihsan, M., & Mahsul, A. (2018). Optimalisasi Tingkat Pertumbuhan (Growth Rate) dan Mortalitas (Survival Rate) Ikan Nila (Oreochromis Niloticus) dengan Enzim Papain Kasar. Biota, 9(2), 190–199. https://doi.org/10.20414/jb.v9i2.47

Indriani, A. D., Prayitno, S. B., & Sarjito. (2014). Penggunaan Ekstrak Jahe Merah (Zingiber officinale var. Rubrum) Sebagai Alternatif Pengobatan Ikan NIla (Oreochromis niloticus) yang Diinfeksi Bakteri Aeromonas hydrophila. Journal of Aquaculture Management and Technology, 3(3), 58–65. Retrieved from https://ejournal3.undip.ac.id/index.php/jamt/article/view/5802

Kerigano, N. K., Chibsa, T. R., Molla, Y. G., Mohammed, A. A., Tamiru, M., Bulto, A. O., Wodaj, T. K., Gebreweld, D. S., & Abdi, A. K. (2023). Phenotypic, molecular detection and antibiogram analysis of Aeromonas Hydrophila from Oreochromis Niloticus (Nile Tilapia) and Ready-To- eat fish products in selected Rift Valley lakes of Ethiopia. BMC Veterinary Research, 19(1). https://doi.org/10.1186/s12917-023-03684-3

Kusdarwati, R., Rozi, D Dinda, N., & Nurjanah, I. (2018). Antimicrobial resistance prevalence of Aeromonas hydrophila isolates from motile Aeromonas septicemia disease. IOP Conference Series: Earth and Environmental Science, 137(1). https://doi.org/10.1088/1755-1315/137/1/012076

Levia, K., Waspodo, S., & Astriana, B. H. (2021). Uji Efektivitas Anti Bakteri Ekstrak Biji Pepaya (Carica papaya L.) Terhadap Kelangsungan Hidup Ikan Nila (Oreochromisniloticus) Pasca Infeksi Aeromonas hydrophila. Jurnal Perikanan Unram, 11(2), 195–208. https://doi.org/10.29303/jp.v11i2.255

Ma, S., Dong, Y., Wang, N., Liu, J., Lu, C., & Liu, Y. (2020). Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system. Veterinary Research, 51(1). https://doi.org/10.1186/s13567-020-00794-w

Murwantoko, Rozi, Istiqomah, I., & Nitimulyo, K. H. (2013). Isolasi, Karakterisasi, dan Patogenitas Bakteri Penyebab Penyakit Pada Gurami (Osphronemus goramy) di Kabupaten Bantul. Jurnal Perikanan (Journal of Fisheries Sciences) All Right Reserved, 2, 83–90. https://doi.org/10.22146/jfs.9102

Ngamkala, S., Satchasataporn, K., Setthawongsin, C., & Raksajit, W. (2020). Histopathological study and intestinal mucous cell responses against Aeromonas hydrophila in Nile tilapia administered with Lactobacillus rhamnosus GG. Veterinary World, 13(5), 967–974. https://doi.org/10.14202/vetworld.2020.967-974

Nivelle, R., Gennotte, V., Kalala, E. J. K., Ngoc, N. B., Muller, M., Mélard, C., & Rougeot, C. (2019). Temperature preference of Nile tilapia (Oreochromis niloticus) juveniles induces spontaneous sex reversal. PLoS ONE, 14(2). https://doi.org/10.1371/journal.pone.0212504

Qu, B., Zhao, H., Chen, Y., & Yu, X. (2022). Effects of low-light stress on aquacultural water quality and disease resistance in Nile tilapia. PLoS ONE, 17(5 May). https://doi.org/10.1371/journal.pone.0268114

Rachmawati, D., & Prihanto, A. A. (2019). Effect of Papain Enzym Supplementation on Growth Performance and Nutrient Utilization of Catfish (Pangasius hypopthalmus). Malays. Appl. Biol, 48(5), 1–10. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/1581

Rifaath, M. (2022). Effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro. Bioinformation, 18(3), 289–292. https://doi.org/10.6026/97320630018289

Rozi, R., C. Prijantono, R., Sudarno, & Kusdarwati, R. (2022). Pengaruh Asap Cair Tempurung Kelapa (Cocos nucifera) terhadap Hematologi Ikan Nila (Oreochromis niloticus) yang Diuji Tantang Bakteri Aeromonas hydrophila. Journal of Aquaculture Science, 7(1). https://doi.org/10.31093/joas.v7i1.236

Rozi, Rahayu, K., Daruti, D. N., & Stella, M. S. P. (2018). Study on characterization, pathogenicity and histopathology of disease caused by Aeromonas hydrophila in gourami (Osphronemus gouramy). IOP Conference Series: Earth and Environmental Science, 137(1). https://doi.org/10.1088/1755-1315/137/1/012003

Safitri, D., Roanisca, O., & Mahardika, R. G. (2021). Potensi Ekstrak Daun Senduduk (Melastoma malabathricum Linn.) Sebagai Antibakteri terhadap Pseudomonas aeruginosa dan Staphylococcus aureus. Chimica et Natura Acta, 9(2), 74–80. https://doi.org/10.24198/cna.v9.n2.34582

Salam, N. I., Sambu, A. H., & Heriawan, E. (2020). Optimasi Penggunaan Enzim Papain Pada Pakan Keong Terhadap Kelangsungan Hidup dan Pertumbuhan Ikan Patin (Pangasius sp.). OCTOPUS : Jurnal Ilmu Perikanan, 9(2), 66–71. https://doi.org/10.26618/octopus.v9i2.7068

Saleh, A., Elkenany, R., & Younis, G. (2021). Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes. Biocontrol Science, 26(3), 167–176. https://doi.org/10.4265/bio.26.167

Sharma, A., Sharma, R., Sharma, M., Kumar, M., Barbhai, M. D., Lorenzo, J. M., Sharma, S., Samota, M. K., Atanassova, M., Caruso, G., Naushad, M., Radha, Chandran, D., Prakash, P., Hasan, M., Rais, N., Dey, A., Mahato, D. K., Dhumal, S., & Mekhemar, M. (2022). Carica papaya L. Leaves: Deciphering Its Antioxidant Bioactives, Biological Activities, Innovative Products, and Safety Aspects. In Oxidative Medicine and Cellular Longevit. Hindawi Limited. https://doi.org/10.1155/2022/2451733

Shen, G. P., Ding, Z. N., Dai, T., Feng, J. H., Dong, J. Y., Xia, F., Xu, J. J., & Ye, J. D. (2021). Effect of dietary taurine supplementation on metabolome variation in plasma of Nile tilapia. Animal, 15(3). https://doi.org/10.1016/j.animal.2020.100167

Sherif, A. H., & Kassab, A. S. (2023). Multidrug-resistant Aeromonas bacteria prevalence in Nile tilapia broodstock. BMC Microbiology, 23(1). https://doi.org/10.1186/s12866-023-02827-8

SNI 7550. (2009). Produksi ikan nila (Oreochromis niloticus Bleeker) kelas pembesaran di kolam air tenang. Badan Standardisasi Nasional.

Sychrová, A., Škovranová, G., Čulenová, M., & Bittner Fialová, S. (2022). Prenylated Flavonoids in Topical Infections and Wound Healing. Molecules, 27(14). MDPI. https://doi.org/10.3390/molecules27144491

Tacias-Pascacio, V. G., Castañeda-Valbuena, D., Morellon-Sterling, R., Tavano, O., Berenguer-Murcia, Á., Vela-Gutiérrez, G., Rather, I. A., & Fernandez-Lafuente, R. (2021). Bioactive peptides from fisheries residues: A review of use of papain in proteolysis reactions. International Journal of Biological Macromolecules, 184, 415–428. https://doi.org/10.1016/j.ijbiomac.2021.06.076

Winda, F. R., Suparno, S., & Prasetyo, Z. K. (2023). Antibacterial Activity of Cinnamomum burmannii Extract Against Escherichia coli. Jurnal Penelitian Pendidikan IPA, 9(11), 9162–9170. https://doi.org/10.29303/jppipa.v9i11.4045

Youssef, I. M. I., Saleh, E. S. E., Tawfeek, S. S., Abdel-Fadeel, A. A. A., Abdel-Razik, A. R. H., & Abdel-Daim, A. S. A. (2023). Effect of Spirulina platensis on growth, hematological, biochemical, and immunological parameters of Nile tilapia (Oreochromis niloticus). Tropical Animal Health and Production, 55(4). https://doi.org/10.1007/s11250-023-03690-5

Zaher, H. A., Nofal, M. I., Hendam, B. M., Elshaer, M. M., Alothaim, A. S., & Eraqi, M. M. (2021). Prevalence and antibiogram of vibrio parahaemolyticus and aeromonas hydrophila in the flesh of nile tilapia, with special reference to their virulence genes detected using multiplex pcr technique. Antibiotics, 10(6). https://doi.org/10.3390/antibiotics10060654

Zaragozá, C., Villaescusa, L., Monserrat, J., Zaragozá, F., & Álvarez‐Mon, M. (2020). Potential therapeutic anti‐inflammatory and immunomodulatory effects of dihydroflavones, flavones, and flavonols. Molecules, 25(4), 1–13. https://doi.org/10.3390/molecules25041017

Zhou, H., Gai, C., Ye, G., An, J., Liu, K., Xu, L., & Cao, H. (2019). Aeromonas hydrophila, an emerging causative agent of freshwater-farmed whiteleg shrimp litopenaeus vannamei. Microorganisms, 7(10). https://doi.org/10.3390/microorganisms7100450

Author Biographies

Dian Puspitasari, Universitas Asahan

Jeki, Universitas Asahan

Intan Zahar, Universitas Asahan

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