Isolation and Identification of Acid-Forming Bacteria in Arabica Coffee Fruit (Coffea arabica) During Wet Fermentation
DOI:
10.29303/jppipa.v10i8.7335Published:
2024-08-31Issue:
Vol. 10 No. 8 (2024): AugustKeywords:
Acetic acid bacteria, arabica coffee, fermentation, lactic acid bacteriaResearch Articles
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Abstract
The distinctive flavor and aroma possessed by Arabica coffee are an attraction for coffee enthusiasts. Post-harvest processing of coffee such as coffee fruit fermentation also contributes to the formation of the flavor and aroma of Arabica coffee. In this study, spontaneous fermentation was conducted by adding distilled water, with treatments on fermentation days 4 and 7 aimed at detecting acid-forming bacteria that played a role in the fermentation process. The bacterial isolates were characterized through macroscopic and microscopic observations, physiological, and biochemical tests. Identification of bacterial isolates was performed using the profile matching method based on Bergey’s Manual of Systematic Bacteriology (Holt et al., 1994) and Axellson (2004) until the genus level. The identified bacterial genera were Asaia, Gluconobacter, Neoasaia, Saccharibacter, and Lactobacillus.
References
Abubakar, Y., Widayat, H. P., Muzaifa, M., & Mega, F. A. (2020). Isolasi Dan Identifikasi Bakteri Asam Asetat Dari Fermentasi Kakao Aceh. Jurnal Teknologi Pertanian Andalas, 24(1), 23. https://doi.org/10.25077/jtpa.24.1.23-28.2020
Axelsson, L. (2004). Lactic Acid Bacteria: Microbiological and Functional Aspect (Third edit). New York: Marcel Dekker, Inc.
Bansal, S., Singh, A., Mangal, M., & Sharma, S. K. (2013). Isolation and characterization of lactic acid bacteria from fermented foods. Vegetos, 26(2), 325–330. https://doi.org/10.5958/j.2229-4473.26.2.092
Cassimiro, D. M. de J., Batista, N. N., Fonseca, H. C., Naves, J. A. O., Dias, D. R., & Schwan, R. F. (2022). Coinoculation of lactic acid bacteria and yeasts increases the quality of wet fermented Arabica coffee. International Journal of Food Microbiology, 369, 109627. https://doi.org/10.1016/j.ijfoodmicro.2022.109627
Chandra, L. Y. K., Linggarweni, B. I., & Novida, S. (2023). Analisis Pendapatan Usaha Kopi Bubuk Arabika di Desa Sajang Kecamatan Sembalun Kabupaten Lombok Timur. Jurnal Ekonomi Dan Bisnis, 3(2), 148–155. https://doi.org/10.56145/jurnalekonomidanbisnis.v3i2.71
de Carvalho Neto, D. P., de Melo Pereira, G. V., de Carvalho, J. C., Soccol, V. T., & Soccol, C. R. (2018). High-Throughput rRNA Gene Sequencing Reveals High and Complex Bacterial Diversity Associated with Brazilian Coffee Beans Fermentation. Food Technology and Biotechnology, 56(1), 90–95. https://doi.org/10.17113/ftb.56.01.18.5441
Evangelista, S. R., Miguel, M. G. da C. P., Silva, C. F., Pinheiro, A. C. M., & Schwan, R. F. (2015). Microbiological diversity associated with the spontaneous wet method of coffee fermentation. International Journal of Food Microbiology, 210, 102–112. https://doi.org/10.1016/j.ijfoodmicro.2015.06.008
Finanda, A., Hadari Nawawi, J. H., & Barat, K. (2021). Isolasi Dan Karakterisasi Genus Bakteri Asam Laktat Dari Fermentasi Daging Buah Pisang Kepok (Musa paradisiaca L.). Protobiont, 10(2), 37–41. https://doi.org/10.26418/protobiont.v10i2
Gebreselassie, N., Abay, F., & Beyene, F. (2016). Biochemical and molecular identification and characterization of lactic acid bacteria and yeasts isolated from Ethiopian naturally fermented buttermilk. Journal of Food Science and Technology, 53(1), 184–196. https://doi.org/10.1007/s13197-015-2049-z
Gomes, R. J., Borges, M. de F., Rosa, M. de F., Castro-Gómez, R. J. H., & Spinosa, W. A. (2018). Acetic Acid Bacteria in the Food Industry: Systematics, Characteristics and Applications. Food Technology and Biotechnology, 56(2), 129–151. https://doi.org/10.17113/ftb.56.02.18.5593
Gordon, J. M., & Polle, J. E. W. (2007). Ultrahigh bioproductivity from algae. Applied Microbiology and Biotechnology, 76(5), 969–975. https://doi.org/10.1007/s00253-007-1102-x
Gualliman, J. M., & Albert, M. (2011). Molecular Wine Microbiology Chapter 9: Acetid Acid Bacteria. Academi Press.
Haile, M., & Kang, W. H. (2019). The Role of Microbes in Coffee Fermentation and Their Impact on Coffee Quality. Journal of Food Quality, 2019, 1–6. https://doi.org/10.1155/2019/4836709
Halil, A., & Sahlan. (2023). Commodity Marketing Supply Chain Analysis Arabic Coffee. Jurnal Penelitian Pendidikan IPA, 9(7), 5111–5116. https://doi.org/10.29303/jppipa.v9i7.3985
Holt, G. J., Noel, R. K., Peter, H. A. S., James, Ts., & Stanley, Tw. (1994). Bergey’s Manual of Determinative Bacteriology (Ninth). Waverly Company.
Jojima, Y., Mihara, Y., Suzuki, S., Yokozeki, K., Yamanaka, S., & Fudou, R. (2004). Saccharibacter floricola gen. nov., sp. nov., a novel osmophilic acetic acid bacterium isolated from pollen. International Journal of Systematic and Evolutionary Microbiology, 54(6), 2263–2267. https://doi.org/10.1099/ijs.0.02911-0
Kadere, T. T., Miyamoto, T., Oniang’O, R. K., Kutima, P. M., & Njoroge, S. M. (2008). Isolation and identification of the genera Acetobacter and Gluconobacter in coconut toddy (mnazi). African Journal of Biotechnology, 7(16), 2963–2971. https://doi.org/10.5897/AJB08.390
Khatoon, H., Chavan, D. D., Anokhe, A., & Kalia, V. (2022). Catalase Test: A Biochemical Protocol for Bacterial Identification. AgriCos E-Newsletter, 03(1), 53–55. Retrieved from https://www.researchgate.net/publication/357604311_Catalase_Test_A_Biochemical_Protocol_for_Bacterial_Identification
Kosasi, C., Lolo, W. A., & Sudewi, S. (2019). Isolasi dan Uji Aktivitas Antibakteri dari Bakteri Yang Berasosiasi dengan Alga Turbinaria ornata (Turner) J. Agardh Serta Identifikasi Secara Biokimia. PHARMACON, 8(2), 351. https://doi.org/10.35799/pha.8.2019.29301
Lee, B.-H., Huang, C.-H., Liu, T.-Y., Liou, J.-S., Hou, C.-Y., & Hsu, W.-H. (2023). Microbial Diversity of Anaerobic-Fermented Coffee and Potential for Inhibiting Ochratoxin-Produced Aspergillus niger. Foods, 12(15), 2967. https://doi.org/10.3390/foods12152967
Ley, D. J., Gossele, F., & Swings, J. (1989). Genus Acetobacter In Bergey’s Manual of Systematic Bacteriology.
Lisdiyanti, P., Kawasaki, H., Widyastuti, Y., Saono, S., Seki, T., Yamada, Y., Uchimura, T., & Komagata, K. (2002). Kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria. International Journal of Systematic and Evolutionary Microbiology, 52(3), 813–818. https://doi.org/10.1099/00207713-52-3-813
Mahatmanto, T., Sunarharum, W. B., Putri, F. A., Susanto, C. A., Davian, A. O., & Murdiyatmo, U. (n.d.). The microbiology of arabica and robusta coffee cherries: a comparative study of indigenous bacteria with presumptive impact on coffee quality. FEMS Microbiology Letters, 370, 1–8. https://doi.org/10.1093/femsle/fnad024
Malimas, T., Yukphan, P., Takahashi, M., Kaneyasu, M., Potacharoen, W., Tanasupawat, S., Nakagawa, Y., Tanticharoen, M., & Yamada, Y. (2007). Gluconobacter kondonii sp. nov., an acetic acid bacterium in the α-Proteobacteria. The Journal of General and Applied Microbiology, 53(5), 301–307. https://doi.org/10.2323/jgam.53.301
Martati, E. (2007). Identifikasi Mikrobia Pada Fermentasi Biji Kopi Arabika (Coffea arabica Linn). Jurnal Agroteknologi, 1(2), 112–122. Retrieved from https://jurnal.unej.ac.id/index.php/JAGT/article/view/21461
Moreira, I. M. da V., De Figueiredo Vilela, L., Da Cruz Pedroso Miguel, M., Santos, C., Lima, N., & Freitas Schwan, R. (2017). Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor. Molecules, 22(5), 766. https://doi.org/10.3390/molecules22050766
Muzaifa, M., Patria, A., Abubakar, A., Rahmi, F., Hasni, D., & Sulaiman, I. (2016). Kopi Luwak: Produksi, Mutu dan Permasalahannya. Syiah Kuala University Press. https://doi.org/10.52574/syiahkualauniversitypress.336
Nurhasanah, A. K., & Faturrahman, F. (2019). Komunitas Bakteri pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus). Jurnal Sains Teknologi & Lingkungan, 5(1), 1–9. https://doi.org/10.29303/jstl.v5i1.100
Permana, A. H., Yuliana, E., & Nurhalisa, I. A. (2021). Isolasi dan Karakterisasi Morfologi Mikroorganisme pada Proses Fermentasi Kombucha Berbahan Baku Teh Hijau (Camellia sinensis). WARTA AKAB, 45(2), 60–65. https://doi.org/10.55075/wa.v45i2.38
Purwanto, E. H., Frediansyah, A., Fitrianto, N., Palindung, L. S., Marwati, T., Santoso, T. J., Susilawati, L., Khodija, S., Rachmawati, F., & Wibowo, N. A. (2024). Partial purification and pectinase activity of lactic acid bacteria and pectinolytic bacteria consortium. IOP Conference Series: Earth and Environmental Science, 1377(1), 012048. https://doi.org/10.1088/1755-1315/1377/1/012048
Rahayu, H. M., & Setiadi, A. E. (2023). Isolation and Characterization of Indigenous Lactic Acid Bacteria from Pakatikng Rape, Dayak’s Traditional Fermented Food. Jurnal Penelitian Pendidikan IPA, 9(2), 920–925. https://doi.org/10.29303/jppipa.v9i2.2801
Rahmadani, S. Y., Periadnadi, P., & Nurmiati, N. (2020). Isolasi Dan Karakterisasi Isolat Bakteri Indigenous Pemfermentasi Pulp Tiga Varietas Kakao (Theobroma cacao L.) (Isolation and Characterizations of Indigenous Fermenting Bacteria from Pulp of Three Cocoa Varieties (Theobroma cacao, L.)). Biopropal Industri, 11(1), 49. https://doi.org/10.36974/jbi.v11i1.5685
Ribeiro, E., Rocha, T. de S., & Prudencio, S. H. (2021). Potential of green and roasted coffee beans and spent coffee grounds to provide bioactive peptides. Food Chemistry, 348, 129061. https://doi.org/10.1016/j.foodchem.2021.129061
Rocha, R. A. R., Cruz, M. A. D. da, Silva, L. C. F., Costa, G. X. R., Amaral, L. R., Bertarini, P. L. L., Gomes, M. S., & Santos, L. D. (2024). Evaluation of Arabica Coffee Fermentation Using Machine Learning. Foods, 13(3), 454. https://doi.org/10.3390/foods13030454
Saputri, M., Lioe, H. N., & Wijaya, C. H. (2020). Pemetaan Karakteristik Kimia Biji Kopi Arabika Gayo Dan Robusta Gayo. Jurnal Teknologi Dan Industri Pangan, 31(1), 76–85. https://doi.org/10.6066/jtip.2020.31.1.76
Sari, N. A. Y., Sumardi, Salman, Tambunan, I. J., Dachi, K., & Julianty, S. M. (2021). Fermentasi Seduhan Kopi Arabika Dengan Bakteri Lactobacillus Casei Dan Ragi Saccharomyces Cerevisiae Dan Uji Toksisitas. Jurnal Indah Sains Dan Klinis, 2(3), 7–16. https://doi.org/10.52622/jisk.v2i3.32
Sayuti, A., Erina, & Baniar, L. (2017). Isolasi Dan Identifikasi Bakteri Asam Laktat (BAL) Genus Lactobacillus dari Fese Orangutan Sumatera Di Kebun Binatang Kasang Kulim Banginang Riau. Jimvet, 01(3), 351–359. https://doi.org/10.21157/jim%20vet..v1i3.3342
Siahaan, C. O., Lister, I. N. E., Girsang, E., & Fachrial, E. (2023). Test of Antioxidants and Alpha-glucosidase Inhibitors from Lactic Acid Bacteria in Fermentation of Soy Milk (Soygurt). Jurnal Penelitian Pendidikan IPA, 9(9), 7000–7013. https://doi.org/10.29303/jppipa.v9i9.4663
Silamba, I., Salengke, S., Adiansyah, & Hasizah, A. (2024). Enhancing coffee sensory quality: unleashing synergistic effects of selected microorganism starter cultures and ohmic heating technology. IOP Conference Series: Earth and Environmental Science, 1356(1), 012034. https://doi.org/10.1088/1755-1315/1356/1/012034
Sulaiman, I., & Hasni, D. (2022). Microorganism growth profiles during fermentation of Gayo Arabica wine coffee. IOP Conference Series: Earth and Environmental Science, 951(1), 012076. https://doi.org/10.1088/1755-1315/951/1/012076
Tjahjaningsih, W., Masithah, E. D., Pramono, H., & Suciati, P. (2016). Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik
[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Pro. Jurnal Ilmiah Perikanan Dan Kelautan, 8(2), 94–108. https://doi.org/10.20473/jipk.v8i2.11182
Todhanakasem, T., Van Tai, N., Pornpukdeewattana, S., Charoenrat, T., Young, B. M., & Wattanachaisaereekul, S. (2024). The Relationship between Microbial Communities in Coffee Fermentation and Aroma with Metabolite Attributes of Finished Products. Foods, 13(15), 2332. https://doi.org/10.3390/foods13152332
Tsaaqifah, H., Fahrurrozi, F., & Meryandini, A. (2023). Selection Of Lactic Acid Bacteria as Starter Culture for Cocoa Fermentation (Theobroma Cacao L.). Jurnal Penelitian Pendidikan IPA, 9(2), 825–831. https://doi.org/10.29303/jppipa.v9i2.3045
Wang, Y., Wu, J., Lv, M., Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021). Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Frontiers in Bioengineering and Biotechnology, 9(5), 1–19. https://doi.org/10.3389/fbioe.2021.612285
Widodo. (2019). Bakteri Asam Laktat Strain Lokal. Yogyakarta: Gadjah Mada University Press.
Williams, W. S., & Cannon, R. E. (1989). Alternative Environmental Roles for Cellulose Produced by Acetobacter xylinum. Applied and Environmental Microbiology, 55(10), 2448–2452. https://doi.org/10.1128/aem.55.10.2448-2452.1989
Yamada, Y., & Yukphan, P. (2008). Genera and species in acetic acid bacteria. International Journal of Food Microbiology, 125(1), 15–24. https://doi.org/10.1016/j.ijfoodmicro.2007.11.077
Yukphan, P., Malimas, T., Potacharoen, W., Tanasupawat, S., Tanticharoen, M., & Yamada, Y. (2005). Neoasaia chiangmaiensis gen. nov., sp. nov., a novel osmotolerant acetic acid bacterium in the .ALPHA.-Proteobacteria. The Journal of General and Applied Microbiology, 51(5), 301–311. https://doi.org/10.2323/jgam.51.301
Yunita, Ismail, Y. S., & Maha, F. W. (2017). Potensi air nira aren (Arenga pinnata Merr.) sebagai sumber isolat bakteri asam asetat (BAA). Jurnal BIOLEUSER, 1(3), 134–138. Retrieved from https://jurnal.usk.ac.id/bioleuser/article/view/10496
Zulkifli, L., Jekti, D. S. D., & Bahri, S. (2018). Isolasi, Karakterisasi Dan Identifikasi Bakteri Endofit Kulit Batang Srikaya (Annona Squamosa) Dan Potensinya Sebagai Antibakteri. Jurnal Penelitian Pendidikan IPA, 4(1). https://doi.org/10.29303/jppipa.v4i1.98
Author Biographies
Qothrunnada Amira Salma, Universitas Mataram
Sarkono, University of Mataram
Aida Muspiah, Universitas Mataram
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