LC-HRMS Analysis of Abelmoschus Manihot Medik from Palu of Central Sulawesi

Authors

Rafni Mahyudin Paliwang , Viani Anggi , Rezky Yanuarty , Yasinta Rakanita

DOI:

10.29303/jppipa.v10i9.8948

Published:

2024-09-25

Issue:

Vol. 10 No. 9 (2024): September

Keywords:

Abelmoschus, LC-HRMS, Palu City

Research Articles

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Paliwang, R. M., Anggi, V., Yanuarty, R., & Rakanita, Y. (2024). LC-HRMS Analysis of Abelmoschus Manihot Medik from Palu of Central Sulawesi. Jurnal Penelitian Pendidikan IPA, 10(9), 6862–6868. https://doi.org/10.29303/jppipa.v10i9.8948
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Abstract

Abelmoschus manihot (L.) Medik is one of the traditional medicinal plants from Palu City, Central of Sulawesi with the Malvaceae plant family and commonly is a traditional medicinal plants recognized fot its therapeutic potential, particularly in antioxidant, anti-inflammatory and wound healing applications. This research aims to identify and characterize the chemical compounds present in Abelmoschus manihot (L.) Medik plant from Palu City, Central of Sulawesi using  liquid chromatography high resolution mass spectrometry (LC-HRMS). This research was collected fresh plant, designed using ethanol extraction from Abelmoschus manihot (L.) Medik and analyzed using mass spectroscopy (LC-HRMS) with Processing Software – MZMine Ver. 3.9.0. The results of this study  provide new insights into the chemical composition of Abelmoschus manihot (L.) Medik and confirm its potential as a source of bioactive compounds that could be futher explored for pharmaceutical development, namely that it contains the main compound Quercetin with a mass of 303.04, Gossypetin; Myricetin with a mass of 319.04, Quercetin 3-O-alpha-L-rhamnoside with a mass of 449.10 Isoquercetin; Hyperoside; Hyperin; Hirsutrin with a mass of 465.10, Gossypol with a mass of 519.20 and rutin with a mass of 291.08.

References

Abdel-Razek, M. A. M., Abdelwahab, M. F., Abdelmohsen, U. R., & Hamed, A. N. E. (2023). A Review: Pharmacological Activity and Phytochemical Profile of Abelmoschus esculentus (2010-2022). RSC Advances, 13(22), 15280–15294. https://doi.org/10.1039/d3ra01367g

Agraharam, G., Girigoswami, A., & Girigoswami, K. (2022). Myricetin: a Multifunctional Flavonol in Biomedicine. Current Pharmacology Reports, 8(1), 48–61. https://doi.org/10.1007/s40495-021-00269-2

Anand David, A. V., Arulmoli, R., & Parasuraman, S. (2016). Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacognosy Reviews, 10(20), 84–89. https://doi.org/10.4103/0973-7847.194044

Anggi, V. (2021). Total Flavonoid, Alkaloid and Tannin on Leaves and Stems of Abelmoschus Manihot L. Medik From Palu of Central Sulawesi. Journal of Bio Innovation, 10(1), 109–114. https://doi.org/10.46344/jbino.2021.v010i01.08

Anggi, V., & Adikusuma, W. (2019). Total antioxidant and in-vitro cytotoxic of abelmoschus manihot (L.) medik from palu of central sulawesi and doxorubicin on 4t1 cells line and vero cells. Research Journal of Pharmacy and Technology, 12(11), 5472–5476. https://doi.org/10.5958/0974-360X.2019.00949.1

Anggi, V., & Masyita, A. A. (2022). Combination Effects of Abelmoschus manihot (L.) Medik of N-Hexane extracts and Doxorubicin in Breast cancer 4T1 Cells Line. Research Journal of Pharmacy and Technology, 15(2), 639–642. https://doi.org/10.52711/0974-360X.2022.00105

Arsyad, R., Amin, A., & Waris, R. (2023). Teknik pembuatan dan nilai rendamen simplisia dan ekstrak etanol biji bagore (caesalpinia crista l.) Asal polewali mandar. Makassar Natural Product Journal, 1(3), 2023–2138. https://journal.farmasi.umi.ac.id/index.php/mnpj

Ay, M., Charli, A., Jin, H., Anantharam, V., Kanthasamy, A., & Kanthasamy, A. G. (2016). Quercetin. Nutraceuticals: Efficacy, Safety and Toxicity, January 2019, 447–452. https://doi.org/10.1016/B978-0-12-802147-7.00032-2

Bro, R., & Smilde, A. K. (2014). Principal component analysis. Analytical Methods, 6(9), 2812–2831. https://doi.org/10.1039/c3ay41907j

Chang, C. C., Houng, J. Y., Peng, W. H., Yeh, T. W., Wang, Y. Y., Chen, Y. L., Chang, T. H., Hung, W. C., & Yu, T. H. (2022). Effects of Abelmoschus manihot Flower Extract on Enhancing Sexual Arousal and Reproductive Performance in Zebrafish. Molecules, 27(7), 1–15. https://doi.org/10.3390/molecules27072218

Chaudhary, P., Janmeda, P., Docea, A. O., Yeskaliyeva, B., Abdull Razis, A. F., Modu, B., Calina, D., & Sharifi-Rad, J. (2023). Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Frontiers in Chemistry, 11(May), 1–24. https://doi.org/10.3389/fchem.2023.1158198

Chumbhale, D. S., & Khyade, M. S. (2022). Pharmacognostic Evaluation and Development of Quality Control Parameters for Root of Abelmoschus manihot (L.) Medik. Pharmacognosy Research, 15(1), 101–111. https://doi.org/10.5530/097484900263

Della Vedova, L., Ferrario, G., Gado, F., Altomare, A., Carini, M., Morazzoni, P., Aldini, G., & Baron, G. (2022). Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Profiling of Commercial Enocianina and Evaluation of Their Antioxidant and Anti-Inflammatory Activity. Antioxidants, 11(6). https://doi.org/10.3390/antiox11061187

Dwiyanti, R. D., Thuraidah, A., & Nurlailah, N. (2023). Phytochemical Analysis by LC-HRMS and Antibacterial Activity Of the Ethanol Extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe). Medical Laboratory Technology Journal, 9(1), 93–100. https://doi.org/10.31964/mltj.v9i1.506

Gao, Y., Liang, Z., Lv, N., Shan, J., Zhou, H., Zhang, J., & Shi, L. (2022). Exploring the total flavones of Abelmoschus manihot against IAV-induced lung inflammation by network pharmacology. BMC Complementary Medicine and Therapies, 22(1), 1–15. https://doi.org/10.1186/s12906-022-03509-0

Gurav, A. V. M. and R. V. (2016). Phytochemical and Nutritional Studies in the Genus Abelmoschus Medik. Intech, 1, 1–13. https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics

Han, H., Xu, B., Amin, A., Li, H., Yu, X., Gong, M., & Zhang, L. (2019). Quercetin‑3‑O‑α‑L‑rhamnopyranoside derived from the leaves of lindera aggregata (Sims) kosterm. Evokes the autophagy‑induced nuclear factor erythroid 2‑related factor 2 antioxidant pathway in human umbilical vein endothelial cells. International Journal of Molecular Medicine, 43(1), 461–474. https://doi.org/10.3892/ijmm.2018.3976

Hou, J., Qian, J., Li, Z., Gong, A., Zhong, S., Qiao, L., Qian, S., Zhang, Y., Dou, R., Li, R., Yang, Y., & Gu, C. (2020). Bioactive compounds from abelmoschus manihot l. Alleviate the progression of multiple myeloma in mouse model and improve bone marrow microenvironment. OncoTargets and Therapy, 13, 959–973. https://doi.org/10.2147/OTT.S235944

Imran, M., Saeed, F., Hussain, G., Imran, A., Mehmood, Z., Gondal, T. A., El-Ghorab, A., Ahmad, I., Pezzani, R., Arshad, M. U., Bacha, U., Shariarti, M. A., Rauf, A., Muhammad, N., Shah, Z. A., Zengin, G., & Islam, S. (2021). Myricetin: A comprehensive review on its biological potentials. Food Science and Nutrition, 9(10), 5854–5868. https://doi.org/10.1002/fsn3.2513

Indrawati, E., & Setijorini, L. E. (2024). Prospects of Gedi Plant (Abelmoschus manihot L.) as a Functional Food and Herbal Medicine. E3S Web of Conferences, 483. https://doi.org/10.1051/e3sconf/202448302004

Kale, S., Kirdat, P., Kale, S., & Dandge, P. (2022). Phytochemical Screening With Lc-Hrms Profiling and in Vitro Biological Activities of Argyreia Cuneata (L.) and Argyreia Setosa (L.). Asian Journal of Pharmaceutical and Clinical Research, 15(10), 72–78. https://doi.org/10.22159/ajpcr.2022.v15i10.45502

Li, J., Zhang, J., & Wang, M. (2016). Extraction of flavonoids from the flowers of abelmoschus manihot (l.) medic by modified supercritical co2 extraction and determination of antioxidant and anti-adipogenic activity. Molecules, 21(810), 1–14. https://doi.org/10.3390/molecules21070810

Liao, J. C., Li, C. Y., Teng, F. M., Jian-Chen, Yu, J. Y., Ju, W. Z., & Zou, J. D. (2022). Integrated analysis of comprehensive metabolomics and network pharmacology to reveal the mechanisms of abelmoschus manihot (L.) medik. in the treatment of cisplatin-induced chronic kidney disease. Frontiers in Pharmacology, 13(November), 1–17. https://doi.org/10.3389/fphar.2022.1064498

Lima, G. S., Lima, N. M., Roque, J. V., de Aguiar, D. V. A., Oliveira, J. V. A., dos Santos, G. F., Chaves, A. R., & Vaz, B. G. (2022). LC-HRMS/MS-Based Metabolomics Approaches Applied to the Detection of Antifungal Compounds and a Metabolic Dynamic Assessment of Orchidaceae. Molecules, 27(22). https://doi.org/10.3390/molecules27227937

Luan, F., Wu, Q., Yang, Y., Lv, H., Liu, D., Gan, Z., & Zeng, N. (2020). Traditional Uses, Chemical Constituents, Biological Properties, Clinical Settings, and Toxicities of Abelmoschus manihot L.: A Comprehensive Review. Frontiers in Pharmacology, 11(August). https://doi.org/10.3389/fphar.2020.01068

Mehrbod, P., Abdalla, M. A., Fotouhi, F., Heidarzadeh, M., Aro, A. O., Eloff, J. N., McGaw, L. J., & Fasina, F. O. (2018). Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus. BMC Complementary and Alternative Medicine, 18(1), 1–10. https://doi.org/10.1186/s12906-018-2246-1

Nunez, N., Saurina, J., & Núñez, O. (2024). Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Fingerprinting and Chemometrics for Coffee Classification and Authentication. Molecules, 29(1), 1–23. https://doi.org/10.3390/molecules29010232

Patala, R., & Anggi, V. (2022). Pharmacophore Modeling and Molecular Docking of Flavonoid Derivatives in Abelmoschus manihot Against Human Estrogen Receptor Alpha of Breast Cancer. Sciences of Pharmacy, 1(2), 1–9. https://doi.org/10.58920/sciphar01020001

Petrova, N. V., Chernonosov, A. A., Koval, V. V., Andreeva, V. Y., Erst, A. S., Kuznetsov, A. A., Kulikovskiy, M. S., Wang, W., Yu, S. X., & Kostikova, V. A. (2023). LC–HRMS for the Identification of Quercetin and Its Derivatives in Spiraea hypericifolia (Rosaceae) and Anatomical Features of Its Leaves. Plants, 12(2), 1–10. https://doi.org/10.3390/plants12020381

Rafi, M., Hayati, F., Umar, A. H., Septaningsih, D. A., & Rachmatiah, T. (2023). LC-HRMS-based metabolomics to evaluate the phytochemical profile and antioxidant capacity of Cosmos caudatus with different extraction methods and solvents. Arabian Journal of Chemistry, 16(9), 105065. https://doi.org/10.1016/j.arabjc.2023.105065

Selvaraj, D., Subramanian, A., & Samuel, T. (2020). GC-MS analysis of Abelmoschus manihot (L.) Medik (Malvaceae) leaves. World Journal of Advanced Research and Reviews, 5(2), 67–79. https://doi.org/10.30574/wjarr

Shi, R., Tao, Y., Tang, H., Wu, C., Fei, J., Ge, H., Gu, H. F., & Wu, J. (2023). Abelmoschus Manihot ameliorates the levels of circulating metabolites in diabetic nephropathy by modulating gut microbiota in non-obese diabetes mice. Microbial Biotechnology, 16(4), 813–826. https://doi.org/10.1111/1751-7915.14200

Tandi, J., Roem, M., & Yuliet, Y. (2017). Efek Nefroprotektif Kombinasi Ekstrak Daun Gedi Merah dan Daun Kumis Kucing pada Tikus Induksi Etilen Glikol. Journal Of Tropical Pharmacy And Chemistry, 4(1), 27–34. https://doi.org/10.25026/jtpc.v4i1.129

Taroreh, M., Raharjo, S., Hastuti, P., & Murdiati, A. (2016). Antioxidative Activities of Various Fractions of Gedi’s Leaf Extracts (Abelmoschus Manihot L. Medik). Agriculture and Agricultural Science Procedia, 9, 271–278. https://doi.org/10.1016/j.aaspro.2016.02.112

Vona, R., Pallotta, L., Cappelletti, M., Severi, C., & Matarrese, P. (2021). The impact of oxidative stress in human pathology: Focus on gastrointestinal disorders. Antioxidants, 10(2), 1–26. https://doi.org/10.3390/antiox10020201

Winata, G. M., Hardinsyah, H., Marliyati, S. A., Rimbawan, R., & Andrianto, D. (2024). Phytochemical compounds and antioxidant capacities of Abelmoschus manihot leaf extracts using different solvents. Biodiversitas, 25(3), 942–949. https://doi.org/10.13057/biodiv/d250305

Wu, X., & Wang, F. (2023). Spectrum-Effect Relationship and Component Knock-Out or Knock- In in Total Flavones of Abelmoschus manihot. 1–17. Research Square. https://doi.org/10.21203/rs.3.rs-2905807/v1

Zhang, M., Wu, D., Xu, J., Liu, L., Jiao, W., Yu, J., & Chen, G. (2022). Suppression of NLRP3 Inflammasome by Dihydroarteannuin via the HIF‐1α and JAK3/STAT3 Signaling Pathway Contributes to Attenuation of Collagen-Induced Arthritis in Mice. Frontiers in Pharmacology, 13(April), 1–8. https://doi.org/10.3389/fphar.2022.884881

Author Biographies

Rafni Mahyudin Paliwang, College of Pharmaceutical Sciences Pelita Mas of Central of Sulawesi,

Viani Anggi, College of Pharmaceutical Sciences Pelita Mas of Central of Sulawesi,

Rezky Yanuarty, College of Pharmaceutical Sciences Pelita Mas of Central of Sulawesi,

Yasinta Rakanita, College of Pharmaceutical Sciences Pelita Mas of Central of Sulawesi,

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Copyright (c) 2024 Rafni Mahyudin Paliwang, Viani Anggi, Rezky Yanuarty, Yasinta Rakanita

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