Isolation and Inhibitory Activity Testing of Alpha-Glucosidase Enzyme from Endophytic Bacteria in Kumis Kucing (Orthosiphon aristatus (Blume) Miq.) Leaves
DOI:
10.29303/jppipa.v10i10.9264Published:
2024-10-25Issue:
Vol. 10 No. 10 (2024): OctoberKeywords:
α-Glucosidase enzyme, Endophytic bacteria, LC-MS, Orthosiphon aristatus (Blume) MiqResearch Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
Orthosiphon aristatus (Blume) Miq., commonly known as kumis kucing, is frequently used in traditional Indonesian medicine to treat diabetes, hypertension, rheumatism, gout, and as a diuretic. However, large-scale production of medicinal products from natural sources requires significant raw material and land, creating a need for alternative methods. This study explores the potential of isolating endophytic bacteria from kumis kucing leaves to serve as α-glucosidase enzyme inhibitors, offering a more sustainable approach. Eight bacterial endophytes were isolated and tested using bioautography assays and IC50 measurements to assess their inhibitory activity. Four isolates—KK 1, KK 3, KK 4, and KK 5—demonstrated significant α-glucosidase inhibition, with IC50 values of 41.35 µg/mL, 57.04 µg/mL, 56.70 µg/mL, and 164.16 µg/mL, respectively. Molecular testing identified KK 1 as Priestia aryabhattai, while KK 3 and KK 5 were identified as Priestia megaterium. These findings suggest that endophytic bacteria isolated from Orthosiphon aristatus leaves have the potential to be developed as natural sources of α-glucosidase inhibitors, which are beneficial for diabetes management.
References
Amalia, A., Efendi, K., & Novianti, A. (2024). Curcumin Transethosome Gel: Anti-Inflammatory Activity Test in Carragenan-Induced Sprague Dawley Rat. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 1-9. https://doi.org/10.24071/jpsc.005618
Anjum, N., & Chandra, R. (2015). Endophytic Bacteria: Optimization of Isolation Procedure from Various Medicinal Plants and Their Preliminary Characterization. Asian Journal of Pharmaceutical and Clinical Research, 8(4), 233-238. Retrieved from https://www.researchgate.net/publication/282240458
Antara, Y., Razak, A., Umar, I., Gusman, M., & Efendi, N. (2023). Analysis of the Effect of Providing MNBs Organic Liquid Fertilizer on the Growth of Pueraria Javanica Plants in Coal Mining Areas. Jurnal Penelitian Pendidikan IPA, 9(11), 10317–10329. https://doi.org/10.29303/jppipa.v9i11.5548
Arifin, A., Djide, N., & Muhtar, M. (2024). Antibacterial Activity Test of Noni Leaves (Morinda citrifolia L.) Ethanol Extract Ointment Against Staphylococcus aureus Bacteria. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 10–16. https://doi.org/10.24071/jpsc.
Ariyanti, D., Sauriasari, R., & Yunir, E. (2022). Evaluation of Current Practice of Antibiotic Use and Clinical Outcomes of Community-Acquired Pneumonia Patients with Type 2 Diabetes Mellitus in Indonesia. Indonesian Journal of Pharmacy, 33(4), 583–591. https://doi.org/10.22146/ijp.3572
Atikana, A., Sukmarini, L., Warsito, M. F., Untari, F., Murniasih, T., Rahmawati, S. I., Qodria, L., Siwi, O. R., Ratnakomala, S., & Prasetyoputri, A. (2023). Bioactivity Profiles of Actinobacterium Strain BTA 1-131 (InaCC A1205) Isolated from Indonesian Sponge Melophlus sarassinorum. Indonesian Journal of Pharmacy, 34(2), 280–290 https://doi.org
/10.22146/ijp.6455
Danimayostu, A. A., Martien, R., Lukitaningsih, E., & Danarti, R. (2023). Vitamin D3 and the Molecular Pathway of Skin Aging. Indonesian Journal of Pharmacy, 34(3) 357–371. https://doi.org/10.
/ijp.4929
Faharuddin, A., Said, J., Altim, M. Z., Hamson, Z., & Aisah, S. (2023). Microstructure Characteristics of Chicken Feather Panels as an Alternative Wall Material. Jurnal Penelitian Pendidikan IPA, 9(12), 12063–12072. https://doi.org/10.29303/jppipa.v9i
6317
Faridah, I. N., Perwitasari, D. A., Maer, K., Octapermatasari, R., & Novitasari, L. (2022). Traditional Medicine and Its Impact on Patient Outcomes in Type 2 Diabetes Mellitus Therapy. Indonesian Journal of Pharmacy, 33(4), 621–629. https://doi.org/10.22146/ijp.2541
Grechana, O., Serbin, A., Rudnik, A., & Saliy, O. (2023). Five Flavonoids from Lucerne (Medicago sativa L.) Varieties. Indonesian Journal of Pharmacy, 34(2), 253–260. https://doi.org/10.22146/ijp.3671
Hadi, P., Rachmawatie, S. J., & Masnur, M. (2023). Comparison of Chemical and Biological Control Techniques to Stem Rot Fusarium spp. On Fig Seedlings (Ficus carica L.) as an Effort to Substitute Agrochemical Inputs in Environmentally Friendly Control. Jurnal Penelitian Pendidikan IPA, 9(3), 1209–1216. https://doi.org/10.29303/jppipa.v9i3.
Handayani, D., Hafiza, H., Rustini, R., Putra, P. P., & Syafni, N. (2023). Isolation of Endophytic Fungi with Antimicrobial Activity from Medicinal Plant Rhodomyrtus tomentosa (Aiton) Hassk. Journal of Applied Pharmaceutical Science, 13(9), 190–196. https://doi.org/10.7324/JAPS.2023.143365
Harimurti, S., Hidayaturahmah, R., Arsito, P. N., Febriansah, R., & Widada, H. (2024). Synthesis and Characterization of Ethanolic Extract of Red Betel Leaf as an Antiseptic Gel. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 32–43. https://doi.org/10.
/jpsc.006555
Hati, A. K., Yasin, N. M., Kristina, S. A., & Lazuardi, L. (2023). Educational Curriculum to Improve Clinical Outcomes in Diabetes Mellitus Patients: A Systematic Review. Indonesian Journal of Pharmacy, 34(3), 324–338. https://doi.org/10.22146/ijp.4758
Hawari, F. L., Almadea, T., Hananingsih, K., Baikuni, A., Malik, A., Arifianti, A. E., Ramadon, D., & Tjampakasari, C. R. (2023). Development of Bacterial Cocktail of Strains Staphylococcus hominis, Staphylococcus warneri, Bacillus subtilis, and Micrococcus luteus as Active Ingredients for Skin Care Formula. Indonesian Journal of Pharmacy, 34(2), 236–244. https://doi.org/10.22146/ijp.4174
Hawari, H., Suwardji, S., & Idris, H. (2021). The Role of Biochar and Combination of Inorganic Fertilizers and Biological Fertilizers in Increasing Yield and Levels of Brix Sorghum (Sorghum bicolor (L.) Moench) in Dry Land. Jurnal Penelitian Pendidikan IPA, 7(3), 437–442. https://doi.org/10.29303/
jppipa.v7i3.729
Hidayah, R., Harahap, S. K., Lubis, R. K., Junita, R., Sari, L. N., & Khairul, K. (2023). Monitoring the Biological Aspects of Banded Archer Fish (Toxotes jaculatrix Pallas, 1767) in Bilah River, Labuhanbatu Regency, Indonesia. Jurnal Penelitian Pendidikan IPA, 9(2), 676–680. https://doi.org/10.29303/
jppipa.v9i2.2321
Hwang, W. L., Jagadeesh, K. A., Guo, J. A. et al. (2022). Single-Nucleus and Spatial Transcriptome Profiling of Pancreatic Cancer Identifies Multicellular Dynamics Associated with Neoadjuvant Treatment. Nat Genet, 54, 1178–1191. https://doi.org/10.1038/s41588-022-01134-8
Ikhsan, A. N., Rohman, A., Mustafidah, M., Martien, R., & Lestari, L. A. (2023). The Implementation of Fourier Transform Infrared Spectroscopy Combined with Chemometrics for the Authentication of Patin (Pangasius micronema) Fish Oil Emulsion. Indonesian Journal of Pharmacy, 34(2), 174-181. https://doi.org/10.22146/ijp.3773
Jacinda, A. A., Sriyati, S., & Solihat, R. (2024). Integration of Local Potential of Way Kambas National Park in Developing HOTS-Based Assessment Content in Biological Conservation Courses. Jurnal Penelitian Pendidikan IPA, 10(8), 4623–4633. https://doi.org/
29303/jppipa.v10i8.8364
Juliani, J., Yuliana, N. D., Budijanto, S., Wijaya, C. H., & Khatib, A. (2016). α-Glucosidase Inhibitor and Antioxidant Compounds from Orthosiphon stamineus Benth Using FTIR Based Metabolomics. Jurnal Teknologi dan Industri Pangan, 27(1), 17-30. https://doi.org/10.6066/jtip.2016.27.1.17
Julianus, J., Gunawan-Puteri, M. D. P. T., Wiwengku, R. T. P., Setiawati, A., & Hendra, P. (2023). In Vitro and In Vivo Anti Hyperglycemic Evaluation of Sterculia quadrifida Bark through The Inhibition of Alpha Glucosidase. Jurnal Farmasi Sains dan Komunitas (JFSK), 20(1), 15-21. https://doi.org/10.
/jpsc.005153
Le, C., Nguyen, T. M. H., Ngo, T. K. C., & Tran, T. T. N. (2023). Evaluation of Factors Associated with Beliefs about Antidiabetic Medicine in Outpatients with Type 2 Diabetes at Hue University Hospital. Indonesian Journal of Pharmacy, 34(4), 675-685. https://doi.org/10.22146/ijp.6871
Madonna, M., Sumardjo, S., Amanah, S., & Anwas, E. O. M. (2022). Mobilization of Cyber Extension Participants to Build Household Food Security. Jurnal Penelitian Pendidikan IPA, 8(SpecialIssue), 67–75. https://doi.org/10.29303/jppipa.v8iSpecia
lIssue.2479
Mahata, L. E., Ali, H., Murni, A. W., & Alimuddin, T. (2023). Basil Extract (Ocimum basilicum L) Exhibits Antidiabetic and Hepatoprotective Effects via SIRT1 and Peroxisome Proliferator-Activated Receptors (PPARγ) on Gestational Diabetes Mellitus Rats Model. Indonesian Journal of Pharmacy, 34(1), 36-44. https://doi.org/10.22146/
ijp.4285
Mahyuni, E. L., & Harahap, U. (2024). The Formulation of Effervescent Granules with Oxalis dehradunensis Raizada Ethanol Extract as an Antioxidant. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 44–50. https://doi.org/10.24071/jpsc.
Meriyani, H., Sanjaya, D. A., Juanita, R. A., & Ernawati, D. K. (2023). A Narrative Review of Staphylococcus hominis Resistance Pattern: Multidrug-and Possible Extensively Drug-Resistance. Indonesian Journal of Pharmacy, 34(3), 339–356. https://doi.org/10.22146/ijp.5429
Mubarak, A., Rahayu, E. S., & Retnoningsih, A. (2023). Diversity of Edible Fruit Species in Gunung Leuser National Park Area, Aceh Tamiang. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 372–378. https://doi.org/10.29303/jppipa.v9iSpecialIssue.6164
Najim, H. D., Mohammed, M. M., & Rahmah, A. M. (2024). Impact of Dapagliflozin as Add-on Therapy on Glycemic Status and Quality of Life in Type 2 Diabetic Patients. Indonesian Journal of Pharmacy, 35(1), 154–161. https://doi.org/10.22146/ijp.8501
Narasukma, E., Cahyani, I. M., Retnaningsih, C., Ananingsih, V. K., Prahasiwi, M. S., Nugraheni, B., & Sari, A. D. N. I. (2021). The Effect of Aqueous Fraction of Clinacanthusnutans (Burm. F.) Extract Microcapsules on Rat Blood Glucose Level. Indonesian Journal of Pharmacy, 32(1), 126–131. https://doi.org/10.22146/ijp.1230
Nastiti, C. M. R. R., Michelina, E., Wijayanti, F. R., & Gani, M. R. (2024). Evaluation of Diabetic Wound Healing Activity of Novel Quercetin Topical Preparations. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 51-59. https://doi.org/10.24071/jpsc.007288
Nguyen, N. N. T., Vo, D. L., Le, T. T. Y., & Nguyen, T. T. D. (2024). Optimization of Spray Drying Process of Mangiferin Extract from Mango Leaf (Mangifera indica L.) and Encapsulation for Diabetes Treatment. Indonesian Journal of Pharmacy, 35(2), 259-271 https://doi.org/10.22146/ijp.8267
Ningrum, V. D. A., Yuantari, R., Kurniawan, I. N., & Wahyuni, D. (2023). The Effect of Education Models on Changed Misconceptions about Long-Term Medication Inducing Renal Impairment. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 20(1), 29–39. https://doi.org/10.24071/jpsc.003888
Nostro, A., Germano, M. P., Angelo, V. D., Marino, A., & Cannatelli, M.A. (2000) Extraction Methods and Bioautography for Evaluation of Medicinal Plant Antimicrobial Activity. Letters in Applied Microbiology, 30, 379-384. https://doi.org/10.1046
/j.1472-765x.2000.00731.x
Nova, M. L., & Virginia, D. M. (2023). Why Does the Poor Glycemic Control Among Type 2 Diabetes Mellitus Patients Remain High in Southeast Asia? Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 20(2), 201–209. https://doi.org/10.24071/jpsc.006214
Nurjanah, A., Herlina, K., & Ertikanto, C. (2023). Development of Natural Disaster Mitigation LKPD Based on PJBL-STEM Assisted by Micro: Bit to Increase Self Awareness and Creative Problem Solving. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 506–513. https://doi.org/10.29303
/jppipa.v9iSpecialIssue.6124
Octriany, Y., & Ratnawulan, R. (2023). Effect E. coli Bacteria Concentration as Self Healing on Compressive Strength and Hydrophobic Properties on Micro Cracks of Concrete. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 989–997. 10.29303/jppipa.v9iSpecialIssue.5911
Orbayinah, S., Widada, H., Anindita, N. S., & Mohdar, A. F. (2024). Isolation and Protein Profile of Chicken, Pork and Processed Products Nugget with Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Method. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 21(1), 60–67. https://doi.org/10.24071/jpsc.007099
Panduwiguna, I., Sauriasari, R., Sartika, R. A. D., & Riyadina, W. (2023). A Diabetes-Specific Questionnaires Validated in Indonesia: A Systematic Review. Indonesian Journal of Pharmacy, 34(4), 541-554. https://doi.org/10.22146/ijp.6225
Paujiah, E., Zulfahmi, I., Affan, J. M., Fina, M., & Nafis, B. (2024). Composition, Conservation Status, and Market Value of Fish Landed at the Labuhan Haji Fishing Port, Aceh, Indonesia. Jurnal Penelitian Pendidikan IPA, 10(7), 4158–4171. https://doi.org/
29303/jppipa.v10i7.8333
Prasetyastuti, P., & Ghozali, D. S. (2021). The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice. Indonesian Journal of Pharmacy, 32(2), 193–200. https://doi.org/10.22146/ijp.1354
Pratiwi, R. K., Mahmudi, M., Faqih, A. R., & Arfiati, D. (2023). Dynamics of Water Quality for Vannamei Shrimp Cultivation in Intensive Ponds in Coastal Areas. Jurnal Penelitian Pendidikan IPA, 9(10), 8656–8664. https://doi.org/10.29303/jppipa.v9i10.4322
Putri, V. S., Ikhsan, A. N., Martien, R., & Adhyatmika, A. (2023). Validation of Uv-Vis Spectrophotometric Method to Determine Drug Release of Quercetin Loaded-Nanoemulsion. Indonesian Journal of Pharmacy, 34(2), 272–279. https://doi.org/10.
/ijp.4454
Riska, J. A. T., Syukur, A., & Zulkifli, L. (2023). Association between Mangrove Types and Some Mangrove Crab Species in West Lombok Sheet Mangrove Ecosystem. Jurnal Penelitian Pendidikan IPA, 9(7), 5610–5619. https://doi.org/10.29303/
jppipa.v9i7.4781
Rismayanti, T., Latipah, I., Jawahir, I., Sari, I. N., & Komala, I. (2024). Biological Perspective Analysis on Education Level and Motivation in the Implementation of Premarital HIV Screening Tests: A Quantitative Study with a Cross-Sectional Design. Jurnal Penelitian Pendidikan IPA, 10(7), 3949–3956. https://doi.org/10.29303/jppipa.v10i7
.8282
Riswanto, F. D. O., Windarsih, A., Putri, D. C. A., & Gani, M. R. (2023). An Integrated Authentication Analysis of Citrus aurantium L. Essential Oil Based on FTIR Spectroscopy and Chemometrics with Tuning Parameters. Indonesian Journal of Pharmacy, 34(2), 205–217. https://doi.org/10.22146/ijp.5225
Rustini, D. R. A., Putra, P. P., Andayani, R., & Dwinatrana, K. (2023). Antibacterial Activity of Endophytic Bacterial Extracts Isolated from Pineapple Peel (Ananas comosusL.). Trop J Nat Prod Res, 7(7), 3320-3324. Retrieved from https://www.tjnpr.org/index.php/home/article/view/2218/2996
Sahila, S., Suhendy, H., & Sukmawan, Y. P. (2023). Wound Healing Effectivity of Ageratum conyzoides L. Leaf Ethanolic Extract (Purple Flower Type), Centella asiatica (L.) Urban Leaf Ethanolic Extract, and Astaxanthin Combination Gel Preparation in Diabetic Animal Model. Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community), 20(1), 78–83. https://doi.org/10.24071/jpsc.003319
Sari, D. Y., & Widyasari, R. (2023). Antioxidant Activity and Irritation Potency of Face Tonic Formulation from Ethanol Fraction of Sappan Wood (Caesalpinia sappan L.). Indonesian Journal of Pharmacy, 34(2), 261–271. https://doi.org/10.
/ijp.4552
Sasongko, H., Nugroho, A. E., Nurrochmad, A., & Rohman, A. (2024). Nephroprotective Effect of Milkfish, Patin, and Snakehead Fish Oil by Suppressing Inflammation and Oxidative Stress in Diabetic Rats. Indonesian Journal of Pharmacy, 35(1), 63–73. https://doi.org/10.22146/ijp.7725
Setyowati, E., & Agustin, H. Y. (2022). The Pindang Fish Quality is Based on Physical, Chemical, and Biological Parameters. Jurnal Penelitian Pendidikan IPA, 8(6), 2764–2771. https://doi.org/10.29303/
jppipa.v8i6.2416
Sukmawati, S., Adnyana, I. M., Supraptha, D. N., & Busaifi, R. (2022). Effect of MVA Indigenous Isolates and Types of Planting Media on Growth and Result of Corn Plant on Dry Land Central Lombok. Jurnal Penelitian Pendidikan IPA, 8(SpecialIssue), 112–116. https://doi.org/10.29303
/jppipa.v8iSpecialIssue.2470
Sumarto, S., Radiati, A., & Seftiatullaeli, N. (2023). Downstreaming Research Results on Food Products as Enhancers of Immunity During the Covid-19 Pandemic through Nutrition Education. Jurnal Penelitian Pendidikan IPA, 9(2), 762–769. https://doi.org/10.29303/jppipa.v9i2.3021
Syamsuri, S., Alang, H., Yusal, M. S., Hastuti, H., & Adriani, A. (2023). Ethnoeconomics of Plants as Traditional Medicine (Ethnomedicine) and Food (Ethnoculinary) of the Bastem Indigenous Community in Luwu Regency. Jurnal Penelitian Pendidikan IPA, 9(10), 9033–9040. https://doi.org/
29303/jppipa.v9i10.4302
Author Biographies
Indah Tamara Herman Putri, Universitas Andalas
Rustini, Andalas University, Padang
Friardi Ismed, Andalas University, Padang
Valdy F Sardi, Andalas University, Padang
Nurwahidatul Arifah, Baiturrahmah University, Padang
License
Copyright (c) 2024 Indah Tamara Herman Putri, Rustini, Friardi Ismed, Valdy F Sardi, Nurwahidatul Arifah
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).