Anatomical and Physiological Responses of M5 Mutant Red Rice G16 from Gamma Irradiation to Drought Stress Condition in Vegetative Phase

Authors

Ria Ayu Chandraningsih , A.A.K. Sudharmawan , Ni Wayan Sri Suliartini , M. Taufik Fauzi , Uyek Malik Yakop

DOI:

10.29303/jossed.v5i2.9082

Published:

2024-10-31

Issue:

Vol. 5 No. 2 (2024): October

Keywords:

Drought stress, Mutant, Anatomy, Physiology, Vegetative

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Chandraningsih, R. A., Sudharmawan, A., Suliartini, N. W. S., Fauzi, M. T., & Yakop, U. M. (2024). Anatomical and Physiological Responses of M5 Mutant Red Rice G16 from Gamma Irradiation to Drought Stress Condition in Vegetative Phase. Journal of Science and Science Education, 5(2), 121–128. https://doi.org/10.29303/jossed.v5i2.9082

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Abstract

Food security and sustainability are facing challenges due to the increase in population and the large amount of dry land that has not been optimized for utilization. Rice cultivated in paddy fields is a problem that must be considered. The use of local varieties should be further studied because it is one of the efforts to increase plant productivity against drought stress. This study aims to assess the anatomical and physiological responses of M5 mutant G16 red rice from gamma irradiation to drought stress in the vegetative phase. The method used was experimental method with field experiments. The experiment was conducted from November 2023 to July 2024 at the Greenhouse of the Faculty of Agriculture, University of Mataram. The experimental design used was Faktorial Completely Randomized Design (CRD) with two factors, the first factor is drought stress consisting of 33% Field Capacity (K1), 66% Field Capacity (K2), 100% Field Capacity (K3) and the second factor is genotype consisting of Inpago Unram (P1), MD200-G13-18-16 (P2), MD300-G20-16-9-5 (P3), MD200-G24-17-10 (P4), MD300-G27-8-3-5 (P5). The result of this study show that MD200-G24-17-10 (P4) was the most responsive genotype to drought stress conditions in the vegetative phase. This was indicated by a decrease in stomatal density and chlorophyll content, as well as an increase in xylem diameter and stele diameter.

References

Awanis, S., Purwanto, E., & Rahayu, M. (2022). The potential of Central Java local black rice and red rice as drought tolerant cultivars. Web of Conferences IConARD, 1-8. https://doi.org/10.1051/e3sconf/202236104007

Hariyadi, Hidayati, N., Rosawanti, P., Susilo, D. E. H., & Arfianto, F. (2023). Hubungan Tinggi Tanaman, Nisbah Pucuk Akar, Diamete Batang terhadap Berat Buah Cabai di Tanah Gambut. Daun, 10(2), 260-269. https://doi.org/10.33084/daun.v10i2.6218

Hidayati, N., Hendrati, R. L., Triani, A., & Sudjino (2017). Pengaruh Kekeringan Terhadap Pertumbuhan dan Perkembangan Tanaman Nyamplung (Callophylum inophyllum L.) dan Johar (Cassia florida Vah L.) dari Provenan yang Berbeda. Jurnal Pemuliaan Tanaman Hutan. 11(2): 99-111.

Hikmat, M., Hati, D. P., Pratamaningsih, & M. M., Sukarman. (2022). Kajian Lahan Kering Berproduktivitas Tinggi di Nusa Tenggara untuk Pengembangan Pertanian. Jurnal Sumberdaya Lahan, 16(2), 119-133. http://dx.doi.org/10.21082/jsdl.v16n2.2022.119-133

Karlova, R., Boer, D., Hayes, S., & Testerink, C. (2021). Root Plasticity Under Abbiotic Stress. Plant Physiologi, 187(3), 1057-1070. https://doi.org/10.1093/plphys/kiab392

Kim, Y., Chung, Y. S., Lee, E., Tripathi, P., Heo, S., & Kim, K. H. (2020). Root Response to Drought Stress in Rice (Oryza sativa L.). International Journal Molecular Science, 21(4), 1513. https://doi.org/10.3390/ijms21041513

Maisura, Chozin, M. A., Lubis, I., Junaedi, A., Ehara, H. (2015). Laju Asimilasi Bersih dan Laju Tumbuh Relatif Varietas Padi Toleran Kekeringan pada Sistem Sawah. Agrium, 12(1), 10-15. https://doi.org/10.29103/agrium.v12i1.376

Meihana, M. & Lakitan, B. (2022). Dampak Cekaman Muka Air Tanah terhadap Morfologis, Anatomis, dan Fisiologis Tanaman Buncis (Phaseolus vulgaris L.) di Fase Vegetatif. Agroqua. 20(2), 280-291.

Mudhor, M. A., Dewanti, P., Handoyo, T., & Ratnasari, T. (2022). Pengaruh Cekaman Kekeringan terhadap Pertumbuhan dan Produksi Tanaman Padi Hitam Varietas Jeliteng. Jurnal Agrikultura, 33(3), 247–256. https://doi.org/10.24198/agrikultura.v33i3.40361

Mustikarini, E. D., Ardiarini, N. R., Basuki, N., & Kuswanto (2018). Strategi Seleksi Toleransi Kekeringan pada Galur Mutan Beras Merah. Jurnal Agrivita Ilmu Pertanian, 39(1), 91-99. http://dx.doi.org/10.17503/agrivita.v39i1.648c

Nio, S. A., Pirade, M., & Ludong, D. P. M. (2019). Leaf Chlorophyll Content in North Sulawesi (Indonesia) Local Rice Cultivars Subjected to Polyethylene Glycol (PEG) 8000-Induced Water Deficit at the Vegetative Phase. Biodiversitas, 20(9), 2462-2467. 10.13057/biodiv/d200905

Patmi, Y. S., Pitoyo, A., Solichatun, & Sutarno (2020). Effect of Drought Stress on Morphological, anatomical, physiological characteristics of Cempo Ireng Cultivar Mutant Rice (Oryza sativa L.) Strain 51 Irradiated by Gamma-Ray. IOP Science: Journal of Physics, 1436, 1-7.

Phoura, Y., Kamoshita, A., Norisada, M., & Deshmukh, V. (2020). Eco-Physiological Evaluastion of Stele Transversal Area 1 for Rice Root Anatomy and Shoot Growth. Plant Production Science, 23(2), 202-210. https://doi.org/10.1080/1343943X.2020.1727754

Prastyo, K. A. & Laily, A. N. (2015). Uji Konsentrasi Klorofil Daun Temu Mangga (Curcuma mangga Val.) Temulawak (Curcuma xanthorrhiza), dan Temu Hitam (Curcuma aeruginosa) dengan Tipe Kertas Saring yang Berbeda Menggunakan Spektrofotometer. Seminar Nasional Konservasi dan Pemanfaatan Sumber Daya Alam 2015.

Purwanto, E., Parjanto, & Pratama R. A. J. (2021). Study on Growth and Yield of Black Rice M4 Generation Induced by 100 Gamma-Ray for Drought Resistance. IOP Conf. Series: Earth and Environmental Science, 824, 1-8.

Putri, F. W., Suedy, S. W. A., & Darmanti, S. (2017). Pengaruh Pupuk Nanosilika Terhadap Jumlah Stomata, Kandungan Klorofil dan Pertumbuhan Padi Hitam (Oryza sativa L. cv. japonica). Buletin Anatomi dan Fisiologi, 2(1), 72-79. https://doi.org/10.14710/baf.2.1.2017.72-79

Rofidah, N. I., Yulianah, I., & Respatijarti (2018). Korelasi antara Komponen Hasil dengan Hasil pada Populasi F6 Tanaman Cabai Merah Besar (Capsicum annuum L.). Jurnal Produksi Tanaman, 6(2), 230-235.

Salsabila, N., Nandariyah, Yuniastuti, E., Pujiasmanto, B., & Sutarno. (2021). IOP Conf. Series: Earth and Environmental Science, 905, 1-9.

Salsadilla, P. & Hariyono, K. (2022). Respon Pertumbuhan Tiga Varietas Padi Gogo (Oryza sativa L.) pada Berbagai Kondisi Cekaman Kekeringan. Berkalaa Ilmiah Pertanian, 5(1), 45-51. https://doi.org/10.19184/bip.v5i1.29356

Sandar, M. M., Ruangsiri, M., Chutteang, C., Arunyanark, A., Toojinda, T., & Siangliw, J. L. (2022). Root Characterization of Myanmar Upland and Lowland Rice in Relation to Agronomic and Physicological Traits under Drought Stress Condition. Agronomy, 12, 1-19. https://doi.org/10.3390/agronomy12051230

Saputri, D. A., Zakiah, Z., & Turnip, M. (2022). Karakteristik Struktur Anatomi Akar, Batang, dan Daun pada Caladium bicolor cv. Candidum, Caladium bicolor cv. Frieda Hemple, dan Caladium bicolor cv. Postman Joyner. Protobiont, 11(2), 65-70. https://dx.doi.org/10.26418/protobiont.v11i2.63022

Sujinah & Jamil, A. (2016). Mekanisme Respon Tanaman Padi terhadap Cekaman Kekeringan dan Varietas Toleran. Iptek Tanaman Pangan, 11(1), 1-7.

Sudharmawan, A. A. K., Aryana, I. G. P. M., Sudika, I. W., & Sanisah (2008). Perakitan Varietas Unggul Padi Beras Merah Tipe Ideal Melalui Perkawinan Interspesifik Padi Beras Merah Ras Cere dan Bulu Kultivar Lokal NTB. Laporan HB. 54h.

Suliartini, N. W. S., Wangiyana, W., Aryana, I. G. P. M., Sudharmawan, A. A. K. (2020). Radiosensitivity and Seedling Growth of Several Genotypes of Paddy Rice Mutants Irradiated with Gamma Rays at Different Doses. International Journal of Horticulture, Agriculture and Food science (IJHAF), 4(6), 242-247. https://dx.doi.org/10.22161/ijhaf.4.5.5

Sumadji, A. R. & Purbasari, K. (2018). Indeks Stomata, Panjang Akar, dan Tinggi Tanaman sebagai Indikator Kekurangan air pada Tanaman Padi Varietas IR64 dan Ciherang. Jurnal Ilmu Pertanian, Kehutanan, dan Agroekoteknologi, 19(2), 82-85. https://doi.org/10.33319/agtek.v19i2.7

Ujianto, L., Aryana, M., Sudika, I. W., & Sudharmawan, A. A. K. (2020). Teknik Analisis dan Rancangan Persilangan. Mataram University Press. Mataram. ISBN: 978-623-760-855-4.

Umam, R., Sudharmawan, A. A. K., & Sumarjan. (2018). Tampilan Sifat Kuantitatif Beberapa Galur F7 Padi Beras Merah (Oryza sativa L.) Hasil Silang Ganda Indica dengan Javanica. Jurnal Crop Agro, 11(1), 40-47.

Wang, B., Yang, X., Chen, L., Jiang, Y., Bu, H., Jiang, Y., Li, P., & Cao, C. (2022). Physiological Mechanism of Drought-Resistant Rice Coping with Drought Stress. Journal of Plant Growth Regulation, 41, 2638-2651. https://doi.org/10.1007/s00344-021-10456-6

Youssef, M. I. A., Elbagory, M., Elsehely, A. B., El-Gammaal, A. A., El-Denary, M. E., Elat, M. S. A., Talha, I. A., Hazman, M., Nehela, Y., Omara, A. E., & El-Kallawy, W. H. (2023). Biochemical, Anatomical, Genetic, and Yield Assessment of Seven Rice Genotypes (Oryza sativa L.) Subjected to Drought Stress. Agronomy, 13(2542), 1-22. https://doi.org/10.3390/agronomy13102542

Zampieri, E., Pesenti, M., Nocito, F. F., Sacchi, G. A., & Giampiero (2023). Rice Responses to Water Limiting Conditions: Improving Stress Management by Exploiting Genetics and Physiological Processes. Agriculture, 13(464), 1-23. https://doi.org/10.3390/agriculture13020464

Zhahirah, Z., Yenny, R. F., Roidelindho, K., & Romdhonah, Y. (2023). Analisis Korelasi Pertumbuhan dan Hasil Tanaman Tomat Cherry (Solanum lycopersicum var. cerasiforme) secara Hidroponik Sistem Wick. Prosiding Seminar Nasional Pertanian 2023, 33, 338-351.

Author Biographies

Ria Ayu Chandraningsih, University of Mataram

A.A.K. Sudharmawan, University of Mataram

Ni Wayan Sri Suliartini, University of Mataram

M. Taufik Fauzi, University of Mataram

Uyek Malik Yakop, University of Mataram

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Copyright (c) 2024 Ria Ayu Chandraningsih, A.A.K. Sudharmawan, Ni Wayan Sri Suliartini, M. Taufik Fauzi, Uyek Malik Yakop

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