Antioxidant Activity of the Combination of Morinda citrifolia and Alpinia galanga in Juice and Instant Beverage Powder Using the ABTS Method
DOI:
10.29303/jppipa.v12i1.11197Published:
2026-01-25Downloads
Abstract
A combination of Morinda citrifolia and Alpinia galanga is available as a traditional herbal product in two dosage forms, namely juice and instant beverage powder, known as kudu-laos jamu, which potentially exhibits an antioxidant effect. The research novelty of this study lies in the comparison of the antioxidant activity between two dosage forms using the ABTS method. Six formulations were evaluated, including single-plant and combined formulations in both juice (F1–F3) and powder (F4–F6) forms. The antioxidant activity was determined quantitatively by the IC₅₀ value. The results showed that all formulations exhibited antioxidant activity; however, their IC₅₀ values were higher than that of ascorbic acid (< 50 µg/mL), indicating very weak activity (> 200 µg/mL). Statistical analysis showed a significant difference between dosage forms; the IC₅₀ value in instant beverage powder formulations was higher compared with juice formulations. This condition is attributed to dilution by sucrose as the matrix in the powder formulations and to the thermal degradation of bioactive compounds during the processing stage. Therefore, further formulation optimization is required to preserve antioxidant potential in future studies.
Keywords:
ABTS Alpinia galanga Antioxidant Kudu-laos Morinda citrifoliaReferences
Adriani, A., & Pritasari, W. (2024). Literature Review: Jenis dan Manfaat Jamu di Indonesia. Biology and Education Journal, 4(1), 69–79. https://doi.org/10.25299/baej.2024.16976
Andajani, K., Pratiwi, Y., Roekhan, M., R., A., Prastio, B., Maulidina, A., & Marzuki. (2023). Exploring Terminology of The Beauty Jamu and The Beauty Metaphor of East Java Women-Indonesia: An Eco-linguistics Study. Cogent Arts and Humanities, 10(2), 1–19. https://doi.org/10.1080/23311983.2023.2268387 DOI: https://doi.org/10.1080/23311983.2023.2268387
Arfa, N. A. M., & Ghannam, H. A. (2022). Utilization of Rhizome (Alpinia galanga L.) in Improvement of Some Quality Attributes of Some Processed Meat. Food and Nutrition Sciences, 13, 761–779. https://doi.org/10.4236/fns.2022.138055 DOI: https://doi.org/10.4236/fns.2022.138055
Arjita, I. P. D., Saputra, I. P. B. A., & Sukmana, D. J. (2023). Noni (Morinda Citrifolia) Ethanol Extract Lowered Blood Glucose Levels, Increased Glutathione Peroxidase Activity, And Decreased Malondialdehyde in White Rats Model of Diabetes. Jurnal Penelitian Pendidikan IPA, 9(12), 10698–10704. https://doi.org/10.29303/jppipa.v9i12.6076 DOI: https://doi.org/10.29303/jppipa.v9i12.6076
Aziz, I. M., Alfuraydi, A. A., Almarfadi, O. M., Aboul-Soud, M. A. M., Alshememry, A. K., Alsaleh, A. N., & Almajhdi, F. N. (2024). Phytochemical Analysis, Antioxidant, Anticancer, and Antibacterial Potential of Alpinia galanga (L.) Rhizome. Heliyon, 10(17), 1–16. https://doi.org/10.1016/j.heliyon.2024.e37196 DOI: https://doi.org/10.1016/j.heliyon.2024.e37196
Banjarnahor, S. D. S., & Artanti, N. (2014). Antioxidant properties of flavonoids. Medical Journal of Indonesia, 23(4), 239–244. https://doi.org/10.13181/mji.v23i4.1015 DOI: https://doi.org/10.13181/mji.v23i4.1015
Dewi, E. S., & Sari, D. A. (2024). Phytochemical Tests and Antioxidant Activity Tests from Ethanol Extract of Fig Leaves (Ficus carica Linn.) Green Yordan. Food Science and Technology Journal, 7(2), 151–160. https://doi.org/10.25139/fst.vi.8656
Farshchi, A., Guo, M., Gardy, J., Zhang, X., Hassanpour, A., & Naderi, M. (2025). The Effect of Surface Chemistry on The Caking Behaviour of Sucrose Crystals. Journal of Food Engineering, 389, 112386. https://doi.org/10.1016/j.jfoodeng.2024.112386 DOI: https://doi.org/10.1016/j.jfoodeng.2024.112386
Fortin, G. A., Asnia, K. K. P., Ramadhani, A. S., & Maherawati, M. (2021). Review: Minuman Fungsional Serbuk Instan Kaya Antioksidan dari Bahan Nabati. Agrointek: Jurnal Teknologi Industri Pertanian, 15(4), 993–1000. https://doi.org/10.21107/agrointek.v15i4.8977 DOI: https://doi.org/10.21107/agrointek.v15i4.8977
Gulcin, İ. (2020). Antioxidants and Antioxidant Methods: An Updated Overview. Archives of Toxicology, 94(3), 651–715. https://doi.org/10.1007/s00204-020-02689-3 DOI: https://doi.org/10.1007/s00204-020-02689-3
Hou, S., Ma, D., Wu, S., Hui, Q., & Hao, Z. (2025). Morinda citrifolia L.: A Comprehensive Review on Phytochemistry, Pharmacological Effects, and Antioxidant Potential. Antioxidants, 14(3). https://doi.org/10.3390/antiox14030295 DOI: https://doi.org/10.3390/antiox14030295
Ilyasov, I. R., Beloborodov, V. L., Selivanova, I. A., & Terekhov, R. P. (2020). ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways. International Journal of Molecular Sciences, 21, 1131. https://doi.org/10.3390/ijms21031131 DOI: https://doi.org/10.3390/ijms21031131
Kamadia, V. V, Yoon, Y., Schilling, M. W., & Marshall, D. L. (2006). Relationships between Odorant Concentration and Aroma Intensity. Journal of Food Science, 71(3), 193– 197. https://doi.org/10.1111/j.1365-2621.2006.tb15640.x DOI: https://doi.org/10.1111/j.1365-2621.2006.tb15640.x
Kurniati, D., Arifin, H. R., Ciptaningtyas, D., & Windarningsih, F. (2019). Kajian Pengaruh Pemanasan terhadap Aktivitas Antioksidan Buah Mengkudu (Morinda Citrifolia) sebagai Alternatif Sumber Pangan Fungsional Study of Heating Effect on Antioxidant Activity of Noni Fruit (Morinda citrifolia) as an Alternative of Functional Food. Jurnal Teknologi Pangan, 3(1), 20–25. https://doi.org/10.14710/jtp.2019.22562 DOI: https://doi.org/10.14710/jtp.2019.22562
Laksmitawati, D. R., Pratami, D. K., Widowati, W., Kusuma, H. S. W., Wijayanti, C. R., & Rizal, R. (2022). The Potency of Alpinia galanga as Natural Antioxidant. Majalah Obat Tradisional, 27(3), 165–171. https://doi.org/10.22146/mot.72450 DOI: https://doi.org/10.22146/mot.72450
Mawardi, Y. S. A., Pramono, Y. B., & Setiani, B. E. (2016). Kadar Air, Tanin, Warna dan Aroma Off-Flavour Minuman Fungsional Daun Sirsak (Annona Muricata) dengan Berbagai Konsentrasi Jahe (Zingiber Officinale. Jurnal Aplikasi Teknologi Pangan, 5(3), 94–98. https://doi.org/10.17728/jatp.179 DOI: https://doi.org/10.17728/jatp.179
Meilawati, L., Ernawati, T., Triana Dewi, R., Megawati, M., & Sukirno, S. (2021). Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice. Indonesian Journal of Applied Chemistry, 23(2), 55–62. https://doi.org/10.14203/inajac.v23i2.480 DOI: https://doi.org/10.14203/inajac.v23i2.480
Munteanu, I. G., & Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences, 22(7), 1–30. https://doi.org/10.3390/ijms22073380 DOI: https://doi.org/10.3390/ijms22073380
Nadjamuddin, M., Auliah, N., Mulyadin, T., Z., & Andriawan, R. (2023). Test of Antioxidant Effectiveness of Oyster Mushroom (Pleurotus ostreatus) Ethyl Acetate Fraction Cream Formula using FRAP (Ferric Reducing Antioxidant Power) and ABTS (2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid Methods). Jurnal Penelitian Dan Pendidikan IPA, 9(12), 10985–10990. https://doi.org/10.29303/jppipa.v9i12.6118 DOI: https://doi.org/10.29303/jppipa.v9i12.6118
Nursamsiar, K., Marwati, K., N., & Nur, S. (2024). Antioxidant Activities of Ethyl Acetate Extract of Kesambi (Schleichera oleosa L.) with DPPH Method. Indonesian Journal of Pharmaceutical Science and Technology, 11(1), 1–8. https://doi.org/10.24198/ijpst.v11i1.38871 DOI: https://doi.org/10.24198/ijpst.v11i1.38871
Nursamsiar, M., Sami, F. J., Nur, S., Kasmawati, H., & Asnawi, A. (2025). Effect of Solvent Polarity on Antioxidant and Antidiabetic Activity of Kesambi Leaf Extract (Schleichera oleosa L. Jurnal Penelitian Dan Pendidikan IPA, 11(1), 932–937. https://doi.org/10.29303/jppipa.v11i1.9439
Permanasari, D., Sari, A. E., & Aslam, M. (2021). Pengaruh Konsentrasi Gula terhadap Aktivitas Antioksidan pada Minuman Bir Pletok. Aceh Nutrition Journal, 6(1), 9–14. https://doi.org/10.30867/action.v6i1.321 DOI: https://doi.org/10.30867/action.v6i1.321
Rahmawati, I., Hapisah, N., S., N., Sari, E., Rahman, W. A., & Aprilea, N. A. (2024). Evaluation of Aloe Vera (Aloe barbadensis) Instant Powder on Bone and Tooth Development in Pregnant Women. Jurnal Penelitian Dan Pendidikan IPA, 10(12), 11211–11218. https://doi.org/10.29303/jppipa.v10i12.9536 DOI: https://doi.org/10.29303/jppipa.v10i12.9536
Rajivgandhi, G., Saravanan, K., Ramachandran, G., Li, J. L., Yin, L., Quero, F., Alharbi, N. S., Kadaikunnan, S., Khaled, J. M., Manoharan, N., & Li, W. J. (2020). Enhanced Anti-cancer Activity of Chitosan Loaded Morinda citrifolia Essential Oil Against A549 Human Lung Cancer Cells. International Journal of Biological Macromolecules, 164, 4010–4021. https://doi.org/10.1016/j.ijbiomac.2020.08.169 DOI: https://doi.org/10.1016/j.ijbiomac.2020.08.169
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (19999). Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine, 9(100), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3 DOI: https://doi.org/10.1016/S0891-5849(98)00315-3
Sahoo, S., Singh, S., Sahoo, A., Sahoo, B. C., Jena, S., Kar, B., & Nayak, S. (2020). Molecular and Phytochemical Stability of Long Term Micropropagated Greater Galanga (Alpinia galanga. Revealed Suitable for Industrial Applications. Industrial Crops and Products, 148. https://doi.org/10.1016/j.indcrop.2020.112274 DOI: https://doi.org/10.1016/j.indcrop.2020.112274
Samarasinghe, H. G. A. S., Illeperuma, D. C. K., & Gunathilake, K. D. P. P. (2023). An Assessment of The Bioactive Compounds and The Antioxidant, Anti-Inflammatory, and Antidiabetic Potential of Hydro-Methanolic Extracts Derived from Fresh Noni (Morinda citrifolia L.) Fruits Growing in Sri Lanka. Biology and Life Sciences Forum, 26(12), 1–4. https://doi.org/10.3390/Foods2023-15095 DOI: https://doi.org/10.3390/Foods2023-15095
Sardar, B. R., & Singhal, R. S. (2013). Characterization of Co-crystallized Sucrose Entrapped with Cardamom oleoresin. Journal of Food Engineering, 117(4), 521–529. https://doi.org/10.1016/j.jfoodeng.2012.12.011 DOI: https://doi.org/10.1016/j.jfoodeng.2012.12.011
Schugmann, M., & Foerst, P. (2024). Tailoring Crystallization Kinetics in Thin Sucrose Films during Convective Drying: Impact of Temperature and Humidity on Onset, Growth, and Nucleation Rate. Pharmaceutics, 16(10), 1260. https://doi.org/10.3390/pharmaceutics16101260 DOI: https://doi.org/10.3390/pharmaceutics16101260
Setiawan, V., Krisnawan, A. H., Nugroho, J. E., Wahyudi, E. S., Alang, C. A., & Simamora, M. C. (2025). Unlocking Kudu-Laos: Transforming Indonesia’s Ethnic Herbal Drink with SMEs Development Support. Amalee: Indonesian Journal of Community Research and Engagement, 6(1), 93–104. https://doi.org/10.37680/amalee.v6i1.6199 DOI: https://doi.org/10.37680/amalee.v6i1.6199
Sogandi, S., & Rabima, R. (2019). Identification of Active Compound Extracts from Noni Fruit (Morinda citrifolia L.) and Its Potential as Antioxidants. Jurnal Kimia Sains Dan Aplikasi, 22(5), 206–212. https://doi.org/10.14710/jksa.22.5.206-212 DOI: https://doi.org/10.14710/jksa.22.5.206-212
Ulfah, L., Rohmayanti, T., & Nurlaela, R. S. (2023). Karakteristik Kimia dan Sensori Minuman Serbuk Instan Daun Saga (Abrus precatorius L.) dengan Variasi Konsentrasi Sukrosa dan Maltodekstrin. Jurnal Ilmiah Pangan Halal, 5(2), 109–118. https://doi.org/10.30997/jiph.v5i2.10413 DOI: https://doi.org/10.30997/jiph.v5i2.10413
Wang, R., Wang, L., Wu, H., Zhang, L., Hu, X., Li, C., & Liu, S. (2022). Noni (Morinda citrifolia L.) Fruit Phenolic Extract Supplementation Ameliorates NAFLD by Modulating Insulin Resistance, Oxidative Stress, Inflammation, Liver Metabolism and Gut Microbiota. Food Research International, 160, 111732. https://doi.org/10.1016/j.foodres.2022.111732 DOI: https://doi.org/10.1016/j.foodres.2022.111732
Yien, Y. S., Hassan, O., & Zubairi, S. I. (2016). Deodorizing Morinda citrifolia (Mengkudu) Strong Odor Juice by β-Cyclodextrin-Organic Acids Inclusion. Jurnal Teknologi, 78(10), 67–75. https://doi.org/10.11113/jt.v78.7151 DOI: https://doi.org/10.11113/jt.v78.7151
Yu, X., Zhao, M., Liu, F., Zeng, S., & Hu, J. (2013). Antioxidants in Volatile Maillard Reaction Products: Identification and Interaction. LWT-Food Science and Technology, 53, 22–28. https://doi.org/10.1016/j.lwt.2013.01.024 DOI: https://doi.org/10.1016/j.lwt.2013.01.024
Yuliastuti, D. (2022). Formulasi dan Evaluasi Sediaan Serbuk Instan Kombinasi Jahe Emprit (Zingiber officinale Rosc var. amarum) dan Secang (Caesalpinia sappan L. Jurnal Jamu Kusuma, 2(2), 76–82. https://doi.org/10.37341/jurnaljamukusuma.v2i2.45 DOI: https://doi.org/10.37341/jurnaljamukusuma.v2i2.45
Yusransyah, Y., Stiani, S. N., Fathiyati, F., Rachman, S. N., Halimatusyadiah, L., Endah, E., Ismiyati, R., Harpan, A., & Pertiwi, M. (2023). Pelatihan Pembuatan Jamu Instan Pada Masyarakat di Desa Kemanisan Kecamatan Curug Serang Sebagai Upaya Peningkatan Kemandirian Ekonomi Keluarga. Jurnal Abdi Masyarakat Kita, 3(1), 84–94. https://doi.org/10.33759/asta.v3i1.366 DOI: https://doi.org/10.33759/asta.v3i1.366
License
Copyright (c) 2026 Vendra Setiawan, Citra Hayu Adi Makayasa, Alfian Hendra Krisnawan, Cindy Anastasia Alang, Riyanto Budiono

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).






