Effect of Solvent Polarity on Antioxidant and Antidiabetic Activity of Kesambi Leaf Extract (Schleichera oleosa L.)

Authors

Nursamsiar , Marwati , Fitriyanti J. Sami , Syamsu Nur , Henny Kasmawati , Aiyi Asnawi

DOI:

10.29303/jppipa.v11i1.9439

Published:

2025-01-31

Issue:

Vol. 11 No. 1 (2025): January

Keywords:

Antioxidant, Antidiabetic, Kesambi, α-glukosidase

Research Articles

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Nursamsiar, N., Marwati, M., Sami, F. J., Nur, S., Kasmawati, H., & Asnawi, A. (2025). Effect of Solvent Polarity on Antioxidant and Antidiabetic Activity of Kesambi Leaf Extract (Schleichera oleosa L.). Jurnal Penelitian Pendidikan IPA, 11(1), 932–937. https://doi.org/10.29303/jppipa.v11i1.9439

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Abstract

Kesambi (Schleichera Oleosa (L.) is one of the beneficial plants in Indonesia which contains fenolic and flavonoids that exhibit antioxidant properties. The presence of antioxidants in plants could be associated with antidiabetic activity of these plants. This research was conducted to determine the antioxidant and antidiabetic activities of n-hexane, ethyl acetate, acetone and methanol extracts from Kesambi leaves. Extraction was carried out by the ultrasonic assisted extraction (UAE) method. The antioxidant activity was determined quantitatively by DPPH and ABTS method, while the antidiabetic activity was evaluated by using α-glukosidase inhibition method. The IC50 values from the determination of antioxidant and antidiabetic activities were obtained by using Microplate reader at the maximum wavelength. Results of the antioxidant determination DPPH method showed that IC50 values of n-hexane, ethyl acetate, acetone and methanol extracts were 414.1993; 57.7862; 6.33 dan 13.4558 µg/mL, respectively. And for ABTS method showed 162.679; 22.6684; 10.7975; dan 146.869 µg/mL. This indicates the acetone and methanol extracts give the best results in antioxidant activity. Meanwhile, IC50 values of in vitro antidiabetic activity of n-hexane, ethyl acetate, acetone and methanol extracts were 99.3235; 272.8723; 1.6799; dan 24.4653 µg/mL, respectively. Acetone and methanol extracts of kesambi leaf give the best results in antidiabetic activity.

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Author Biographies

Nursamsiar, Almarisah Madani University

Marwati, Almarisah Madani University

Fitriyanti J. Sami, Almarisah Madani University

Syamsu Nur, Almarisah Madani University

Henny Kasmawati, Haluoleo University

Aiyi Asnawi, Universitas Bhakti Kencana

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Copyright (c) 2025 Nursamsiar, Marwati, Fitriyanti J. Sami, Syamsu Nur, Henny Kasmawati, Aiyi Asnawi

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